z****q
发帖数: 18 | 1 做RNA-seq有3’bias，这种结果可以被接受吗 | d********n
发帖数: 15 | 2 You probably used polydT to reverse transcrible them into cDNA, then
performed fragmentation. this gives huge bias on 3/utr. not reliable for
expression level, even worse for analysis of exon junctions.But, it'll be
very good for pcpa (premature cleavage and poly adenylation) analysis. | l********e
发帖数: 415 | 3 看有多严重
很多建library的方法都会有3'-bias. 轻微的bias是完全没关系的.
【在 z****q 的大作中提到】
: 做RNA-seq有3’bias，这种结果可以被接受吗
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