i****o
发帖数: 40 | 1 考虑到是cDNA 又是cancer cells 这有可能是RNA editing的结果 可以看一下是否A/G
change较多 |
i****o
发帖数: 40 | 2 and RNA editing can be present in some cells but not others even in the same
cancer cell line
G
【在 i****o 的大作中提到】
: 考虑到是cDNA 又是cancer cells 这有可能是RNA editing的结果 可以看一下是否A/G
: change较多
|
y******8
发帖数: 1764 | 3 If he or she can rule out other technical possibilities, this would be a
possible and exciting explanation. |
i****o
发帖数: 40 | 4 是的，而且多数Rna editing 非常subtle 要亲自看测序的raw signal才好
【在 y******8 的大作中提到】
: If he or she can rule out other technical possibilities, this would be a
: possible and exciting explanation.
|
y******8
发帖数: 1764 | 5 I would sequence the genomic dna first. If negative, I would do short PCR
and MS analysis for subtle signal of change.
【在 i****o 的大作中提到】
: 是的，而且多数Rna editing 非常subtle 要亲自看测序的raw signal才好
|
z**********8
发帖数: 766 | 6 Hi iFanso,
Thank you very much for your kind reply!
I was told by another people who has same idea with you. I checked the
mutation sites and found only a few harboring A to G mutations. I will
repeat several samples and cell lines again, and do two RT reactions for
getting cDNA, then see what will happen.
Really appreciate your kind information!
Thanks a lot!
G
【在 i****o 的大作中提到】
: 考虑到是cDNA 又是cancer cells 这有可能是RNA editing的结果 可以看一下是否A/G
: change较多
|
n********k
发帖数: 2818 | 7 I could well be wrong...but i still recommend you do some basic homework
like many suggest in the other thread and possibly beyond that before you
jump other hoops or get too excited about it...
Besides, even if there is RNA editing, so what? You need to 1) have a
concrete understanding of your experimental systems to know what to expect(
expected) and to watch for (unexpected), 2) and to have a big picture of
your project, to know: what to chase down(meaningful and feasibility)...
otherwise, this kind of random stuff might get you by a day but certainly
not by a career...just my two cents and good luck...
【在 z**********8 的大作中提到】
: Hi iFanso,
: Thank you very much for your kind reply!
: I was told by another people who has same idea with you. I checked the
: mutation sites and found only a few harboring A to G mutations. I will
: repeat several samples and cell lines again, and do two RT reactions for
: getting cDNA, then see what will happen.
: Really appreciate your kind information!
: Thanks a lot!
:
: G
|
i****o
发帖数: 40 | 8 you are very welcome! btw: yuem0428, neverthink 还有其他id的意见也都是很中肯
的，不过就我个人而言，我喜欢这种 get excited 的感觉，毕竟这是不断的 re-
search 过程中唯一的乐趣阿，没有这种心态也很难坚持下去的。：）
【在 z**********8 的大作中提到】
: Hi iFanso,
: Thank you very much for your kind reply!
: I was told by another people who has same idea with you. I checked the
: mutation sites and found only a few harboring A to G mutations. I will
: repeat several samples and cell lines again, and do two RT reactions for
: getting cDNA, then see what will happen.
: Really appreciate your kind information!
: Thanks a lot!
:
: G
|
