d****i
发帖数: 2346 | 1 http://www.nature.com/nmeth/journal/v10/n2/full/nmeth.2332.html
Detection of histone modifications at specific gene loci in single cells in
histological sections |
p******n
发帖数: 61 | |
X***n
发帖数: 366 | 3 brilliant method.
locus-specific probe coupled with rolling circle amplification. |
d****i
发帖数: 2346 | 4 从方法学上来说可以衍生到所有transcription factor的研究上。。。。。。。 |
d****i
发帖数: 2346 | 5
It is free............
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560316/
【在 p******n 的大作中提到】
: 能不能发个全文啊，我这里下载不了，谢谢！
|
s******y
发帖数: 28562 | 6 我怎么记得这个技术我在好几年前的ASCB 年会上就看到厂商在展示了？
in
【在 d****i 的大作中提到】
: http://www.nature.com/nmeth/journal/v10/n2/full/nmeth.2332.html
: Detection of histone modifications at specific gene loci in single cells in
: histological sections
|
z*t
发帖数: 863 | 7 cool！我觉得除了验证single genomic locus的epigenetic modification，各位大侠
还有什么其他的idea?
【在 d****i 的大作中提到】
: 从方法学上来说可以衍生到所有transcription factor的研究上。。。。。。。
|
l******u
发帖数: 936 | 8 the technology is original from Ulf Landegren lab from Uppsala University,
Sweden. Ulf is a member of the royal swedish academy of sciences, he
invented Pad Lock Probe and Proximity ligation assay. The technologies in
the paper are based on Proximity Ligation assay + RCA (rolling circle
amplification).
in
【在 d****i 的大作中提到】
: http://www.nature.com/nmeth/journal/v10/n2/full/nmeth.2332.html
: Detection of histone modifications at specific gene loci in single cells in
: histological sections
|
h***l
发帖数: 168 | 9 epiLITE from Feng Zhang is also great. |
g*********5
发帖数: 2533 | 10 That one called Duolink.
I tried once and not work.
【在 s******y 的大作中提到】
: 我怎么记得这个技术我在好几年前的ASCB 年会上就看到厂商在展示了？
:
: in
|
s******y
发帖数: 28562 | 11 Yes! That was called dual-link, which I think essentially is the same
technique as showed in this paper.
【在 g*********5 的大作中提到】
: That one called Duolink.
: I tried once and not work.
|
c****1
发帖数: 1095 | 12 这想法不错！
【在 p******n 的大作中提到】
: 能不能发个全文啊，我这里下载不了，谢谢！
|
