m***c
发帖数: 177 | 1 Epigenetics - the fundamental phenomenon of biology
Epigenetics - research tools, models and ...
Epigenetics - influence on future biological and medical research, drug
industry and opportunities
Epigenetics - speak out whatever you know |
g*****y
发帖数: 6325 | 2 a big guy in this field came to our university to talk a few months ago, I
was very inspired! |
m***c
发帖数: 177 | 3 Thanks, but who was it? Bird? |
z*t
发帖数: 863 | 4 i think you're really talking about sth big...hard to begin with,can we
divide it into several part in each topic?
【在 m***c 的大作中提到】
: Epigenetics - the fundamental phenomenon of biology
: Epigenetics - research tools, models and ...
: Epigenetics - influence on future biological and medical research, drug
: industry and opportunities
: Epigenetics - speak out whatever you know
|
z*t
发帖数: 863 | 5 try to“抛砖引玉”
For tools
The most fashionable things nowadays are tons of IP and related high
throughput sequencing ChIP/Me-IP/hm-IP, the application of hybridization
chips are not as popular as several years. But take DNA methylation(the only
staff I'm familar with:)) as example, the Me-IP can highly enrich promoter
region with dense CpG, the hybridization chips developed by Andy Feinberg
are more unbiased. But as everyone knows, the best way is whole genome
bisulfite sequncing, which is expensive and reqiure much input smaple DNA.
When we talk about high throughput sequencing, we are amazed by its power
but forget it usually take more input sample, which confine its usage, but
the PacBio seems get a way around this issue, they can do single molecule
squencing under controled condition, but in our work expirence with PacBio,
this method's accuracy seems to be worse than illumina & their measurement
of modification is based on infered statistics. Taken its short cycle time,
tons of replicate can erase the error and special chemical/biochemical
method can be used to prove the measurment of modification, so I think they
get pretty bright future...
【在 m***c 的大作中提到】
: Epigenetics - the fundamental phenomenon of biology
: Epigenetics - research tools, models and ...
: Epigenetics - influence on future biological and medical research, drug
: industry and opportunities
: Epigenetics - speak out whatever you know
|
s******s
发帖数: 13035 | 6 说到PacBio，当年被他们fancy住了。现在一看，理论再先进，东西
造不好还是不行啊，只能偏向一些小众应用了。
only
promoter
【在 z*t 的大作中提到】
: try to“抛砖引玉”
: For tools
: The most fashionable things nowadays are tons of IP and related high
: throughput sequencing ChIP/Me-IP/hm-IP, the application of hybridization
: chips are not as popular as several years. But take DNA methylation(the only
: staff I'm familar with:)) as example, the Me-IP can highly enrich promoter
: region with dense CpG, the hybridization chips developed by Andy Feinberg
: are more unbiased. But as everyone knows, the best way is whole genome
: bisulfite sequncing, which is expensive and reqiure much input smaple DNA.
: When we talk about high throughput sequencing, we are amazed by its power
|
z*t
发帖数: 863 | 7 要我说东西是有潜力的，需要做的事是很多很多的。。。
【在 s******s 的大作中提到】
: 说到PacBio，当年被他们fancy住了。现在一看，理论再先进，东西
: 造不好还是不行啊，只能偏向一些小众应用了。
:
: only
: promoter
|
m***c
发帖数: 177 | 8 I think you are right. That's why I prefer an open discussion.
【在 z*t 的大作中提到】
: i think you're really talking about sth big...hard to begin with,can we
: divide it into several part in each topic?
|
z*t
发帖数: 863 | 9 tons of excting staff going on:)Someone who's in it may just don't want to
reveal their secret CNS story,haha.
【在 m***c 的大作中提到】
: I think you are right. That's why I prefer an open discussion.
|
z*t
发帖数: 863 | 10 seems you're at mskcc from your id, why don't tell us story going on in New
York, like Dinsaw Partel/ Dave Allis/ Emily Berstein's work
【在 m***c 的大作中提到】
: I think you are right. That's why I prefer an open discussion.
|
|
|
g*****y
发帖数: 6325 | 11 Randy Jirtle
Director of the Epigenetics and Imprinting Laboratory
Duke University
【在 m***c 的大作中提到】
: Thanks, but who was it? Bird?
|
m***c
发帖数: 177 | 12 Well, it's not about the time for me to summarize yet.
New
【在 z*t 的大作中提到】
: seems you're at mskcc from your id, why don't tell us story going on in New
: York, like Dinsaw Partel/ Dave Allis/ Emily Berstein's work
|
z*t
发帖数: 863 | 13 The guy is actually the first to demostrate environment affected mestable
epiallele existed in mammals, the postdoc who work with him is at Baylor now
, he told me about he's not quite in favor of transgenerational epigenetic
inheretance in epidemiological study, he's going to study the early time(
pregnacy/neonatal) will affect the risk of disease by affact the epiallele.
For transgenerational epigenetic inheretance, Yang Shi seems conduct project
with worms,and the recent paper by Oliver Rando gain much public attention,
but has many scientific caveates in the paper.
【在 g*****y 的大作中提到】
: Randy Jirtle
: Director of the Epigenetics and Imprinting Laboratory
: Duke University
|
m***c
发帖数: 177 | 14 Is that it? We expect more! |
z*t
发帖数: 863 | 15 man, that's my little knowledge...just tiny staff, we expect some guys who's
in big bull's lab can give us some filtered information,haha
【在 m***c 的大作中提到】
: Is that it? We expect more!
|
w***m
发帖数: 10498 | 16 when you are talking about Epi, what do you really refer to, DNA
modification or histone modification or something else? |
z*t
发帖数: 863 | 17 let us go to DNA methylation first:)
【在 w***m 的大作中提到】
: when you are talking about Epi, what do you really refer to, DNA
: modification or histone modification or something else?
|
t******e
发帖数: 37 | 18 It is a very hot field, Zymo Research just launched genome scale methylation
profiling platform using Nex-Gen sequencing
【在 z*t 的大作中提到】
: let us go to DNA methylation first:)
|
z*t
发帖数: 863 | 19 效果咋样？花销大不？
我觉得现在急于bisulfite的甲基化测定虽是最solid的办法，但总归是针对群体的，而
即使被普遍认为homogenous的系统如cell line也会存在一定程度的heterogenety，（
比如细胞周期，细胞对特定药物的敏感度）,即使epigenetics研究的就是population
heterogenety但现有手段几乎不可能得到pure homogenous sample，可能最终还是要看
single cell level的epigenetic state
methylation
【在 t******e 的大作中提到】
: It is a very hot field, Zymo Research just launched genome scale methylation
: profiling platform using Nex-Gen sequencing
|
m***c
发帖数: 177 | 20 En, good point. I am working on a mathematical algorithm to solve this type
of problem.
Right, single base resolution is the advantage of bisulfite sequencing. So
many mysteries in this field, that's why 'fifth base' is so tough. |
