t****t
发帖数: 6806 | 1 yes i understand your question. xentar answered it, let me rephrase: for
built-in types, (a=b)=c is legitimate (from syntax point of view. it is
undefined though), so overloaded operators mimic it. why (a=b)=c is
legitimate from the beginning? because it is legitimate in C.
OP? |
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t****t
发帖数: 6806 | 2 i dunno what's your reasonable expectation, but for C users, a=b=c means a=(
b=c), and that's the usual chain assignment. of course (a=b)=c is unusual,
and shouldn't be used because it's undefined for built-in types anyway. for
mimicing built-in type's purpose, it shouldn't be used for class either. no
one said it's a good design. |
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t****t
发帖数: 6806 | 3 yes you can declare like this. then (a=b)=c will fail to compile. but some p
pl think overloaded operators should mimic built-in type, which includes (a=
b)=c. in fact, as a convention, all STL class returns (non-const) reference
to *this in assignment operator. scott meyers mentioned this in
++>. |
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f***t
发帖数: 25 | 4 来自主题: Programming版 - 请教程序 最容易的是mimic keyboard/mouse clicking.
自己写也可以。网上free的带scripts的也很多。 |
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w***z
发帖数: 1848 | 5 来自主题: Programming版 - 请教程序 有没有一些连接呀？ 这个mimic keyboard/mouse clicking是自动的吗？还是要人来激
活？ |
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N********n
发帖数: 8363 | 6 DIV hacks to mimic a pop-up modal dialog. |
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c****e
发帖数: 1453 | 7 If you just want to use streaming, Storm is more mature. Spark is still
using batch processing to mimic streaming.
I feel Storm is largely from CEP(complex event processing). While Spark is a
Scala implemented Dryad + LINQ, with RDD. |
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w**z
发帖数: 8232 | 8 http://www.wired.com/2014/11/microsoft-open-sources-net-says-wi
Satya Nadella’s rapid reinvention of Microsoft continues.
In yet another bid to make up lost ground in the long march to the future of
computing, Microsoft is now open sourcing the very foundation of .NET—the
software that millions of developers use to build and operate websites and
other large online applications—and it says this free code will eventually
run not only on computer servers that use its own Windows operating system,
b... 阅读全帖 |
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N********n
发帖数: 8363 | 9
If it's integer arrays then we can use 2 arrays to mimic hashT.
One array serves as the index. The other serves as the storage. |
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x***u
发帖数: 297 | 10 1> Spyder
Also called spyderlib. I started to use it from 2009. It mimics Matlab
interface. Some early versions have stability issue.
2> ipython notebook
My current favourite. It keeps outputs, graphs in one html page. Great for
reproducible work.
IPython parallel computing clusters is an interesting feature of the
notebook. Potentially, it may boost productivity. I have not tried yet.
3> Enthought / Canopy
Didn't use much. It has free version. If you are .edu user, you can use
professional v... 阅读全帖 |
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a*****g
发帖数: 19398 | 11 By Roland Moore-Colyer
Mon Nov 16 2015, 07:20
http://www.theinquirer.net/inquirer/feature/2434242/facebook-s-
FACEBOOK IS A COMPANY known primarily for its social feed of emotional
statuses, endless emojis, pictures of 'hols with the ladz', and, of course,
a big blue thumbs-up.
Normally associated with tech giants like IBM, Google and Apple, or some
disruptive Tech City startup, artificial intelligence (AI) is not the first
thing to spring to mind when pondering Zuckerberg's 1.5 billion-strong
s... 阅读全帖 |
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h**********c
发帖数: 4120 | 12 can it learn how human forgets |
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F****n
发帖数: 3271 | 13 geohash是给没有spatial data structure的检索系统用的
比如说你只能用一个全文本的搜索引擎，geohash可以用来mimic spatial indexing
你可以把event wx4g0ec1 用所有的prefixes(w, wx, wx4...）索引
这样对任何一级的搜索都能找到
但是如果你自己build一个搜索系统，没有必要用geohash
geohash就是一个很蹩脚的quadtree(essentially a trie), 并不efficient,
应该直接上spatial data structure.
一般来说r-tree is the best. |
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J*****n
发帖数: 48 | 14 beamer is much more flexible than prosper (I like
those navigation buttons),
of course the learning curve for beamer is steep.
prosper mimics ppt style and is easy to learn. in beamer
you can design your own style |
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A**********e
发帖数: 3102 | 15 dvipdfm mimics the behaviors of dvips, then converts meta files to pdf files
.
,
longer
to |
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h****g
发帖数: 439 | 16 在两种同一类型的肿瘤细胞转染mirna mimic，qpcr发现转染后两种细胞中的mirna量都
显著升高。
其中在一个细胞系中发现某蛋白下调，但是在另一个细胞中却不起作用。
刚刚涉入mirna不久，哪位大侠给解释解释这种情况是为啥？ |
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p*****m
发帖数: 7030 | 17 胡同的意思是啥?
我其实觉得larval还好吧 我看你们那儿出来的那个wenbiao chen搞得pancreas那套
发展发展搞个insulin resistance的model应该挺有希望的。。也不需要彻底mimic
人类疾病了 性质差不多也就好了不是 |
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s******y
发帖数: 28562 | 18 Thanks!
The reason people are interested in RGD peptide is because it mimics the
extracellular matrix and binds to the integrins, I believe.
I actually don't really care what kind of peptide it is. However I am kind
of planning to do some pilot experiments by myself and I prefer to use
something that can be handled easily. Therefore, I would like to have
something that is sovlable and cytoplasmic (which will be easy for
expression and require minimal posttranslational modification). While the
ex |
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a****d
发帖数: 1919 | 19 Those are very general questions.
For stem cells/progenitors, self-renewal and proliferation are two distinct
events, although many people don't separate these two when talking about
behavior of stem cells/progenitors. If you ask about proliferation of stem
cells, it seems pretty much similar pathways also regulate tumorgenesis. To
be more specific, differentiation of stem cells/progenitors also mimics EMT,
at certain level. Angiogenesis is ongoing along with progenitors
proliferation, same as t... 阅读全帖 |
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n********k
发帖数: 2818 | 20 I largely concur...nonetheless, I think there are still numerous interesting
stuff to dig there too...
其实对于减肥，最简单和最有效的方法就是健康的生活节奏，运动和饮食，
Very true, virtually for many diseases, not just for obesity, for heart,
brain, immuno....u name it...That's why I call those business are crappy
ones or liar business...My LD keeps telling me to switch to those research
areas---the argument is you are less evil since you would use less harmful
stuff than them so u are doing a great thing to people, sounds righ... 阅读全帖 |
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l**********n
发帖数: 240 | 21 Josef Martin Penninger
Most significant scientific contributions:
Our basic approach is to genetically manipulate and change genes in mice and
to
determine the effects of these mutations in development of the whole
organism and in
diseases. From these mutations we are trying to establish basic principles
of
development and basic mechanisms of disease pathogenesis. My laboratory
focuses on
heart diseases, autoimmune diseases and cancers, and bone diseases. On all
the listed
contributions below I ... 阅读全帖 |
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g*********d
发帖数: 233 | 22 交大招的法国Nobel奖原来是去搞伪科学的
http://www.btsmth.com/show_snapshot.php?en_name=TsinghuaCent&gid=456861
Science诺奖得主吕克·蒙塔尼访谈
French Nobelist Escapes "Intellectual Terror" to Pursue Radical Ideas in
China
Martin Enserink
PARIS—Virologist and Nobel laureate Luc Montagnier announced earlier
this month that, at age 78, he will take on the leadership of a new
research institute at Jiaotong University in Shanghai. What has shocked
many scientists, however, isn't Montagnier's departure from France but
what he pla... 阅读全帖 |
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z*****n
发帖数: 236 | 23 Please contact with the PI if you are interested. If you have any questiosns
, please reply to my post.
Thanks
Description
A Postdoctoral Fellow position is available in the laboratory of Dr. Lakshmi
Nair in the Department of Orthopaedic Surgery at UCHC in the area of
skeletal tissue regeneration. More specifically, the project involves
evaluating the biological and immunological properties of novel
biodegradable materials with appropriate chemistry and mechanical properties
for developing ske... 阅读全帖 |
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l***m
发帖数: 26 | 24 我倾向于找到p53 mimic peptide |
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p*****m
发帖数: 7030 | 25 未必
你可以做两件事，首先可以在dish里诱导分化neuron，至少现在发表的情况看，这些ne
uron是会表现出phenotype的（就是死掉了 两个例子 ALS和rett syndrome的IPS） 不
一定真
的需要多么复杂的体内环境才能表现出来。而且你也可以试图stress这些cultured neu
ron,然后只要他们死的和WT neuron不一样这个差别也可以用来screen gene/drug了
其次你还可以直接把这些iPS打到动物体内去让他们在正确的位置分化成neuron,这样可
以更好的mimic疾病环境。换句话说就是构建一个携带人源疾病组织的动物模型 这个现
在用iPS的lab很多在试。
这两个无论哪一个都比之前的animal model好得多得多。
a
and
domain |
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z*****6
发帖数: 1486 | 26 exactly...specificty is the main concern... Charge is an important reason,
and besides, aptamer is too flexible, can frequently undergo structure
change in response to target or non-preferential target..there are several
papers talking about these issues.
Pegaptanib is a successful example of aptamer used as a drug...
I doubt the specificty of aptamer for a long time. However, in nature, the
special DNA structures, like ribosome RNA, hairpin structures, cloverleaf
structure...can act as specific... 阅读全帖 |
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p*****m
发帖数: 7030 | 27 他作apoptosis pathway的蛋白结构还是很重要地把 解决了apoptosome的组装机制问题
晓东和他之前一起开那个公司做的那个smac mimic还是从smac-IAP的晶体结构出发设
计出来的 那公司后来倒是不知道做得如何就是了
作pp2a也有几篇还有意义的文章
这几年回国之后只知道抓膜蛋白解结构了 我作为外行还没看出内在的科学逻辑是什么
也许有 也许就是为了赶紧发些大paper站稳脚跟把 我反正不知道 |
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f*********e
发帖数: 24 | 28 Relation between MeCP2 and Rett syndrome
1999-10 Nat Gen Huda Y. Zoghbi Rett syndrome is caused by mutations
in X-linked MECP2, encoding methyl-CpG-binding protein 2
Mouse models: whole-body knockout, brain-specific, brain-region-specific,
knock-in,..., over-expression, rescue, ...
2001-03 Nat Gen Adrian Bird A mouse Mecp2-null mutation causes
neurological symptoms that mimic Rett syndrome
2001-03 Nat Gen Rudolf Jaenisch Deficiency of methyl-CpG bindi... 阅读全帖 |
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h********n
发帖数: 4079 | 29 1) I don't know. There is no solid data about cell phone radiation's
physiologic affect.
2) I don't know either.
I guess the simple experiment is to give cultured cell line radiation (mimic
those of cell phone) for months or longer and see any effect. I guess some
people did it but did not find anything interesting.
epi is not the only thing we can look at, but currently epi data is the data
we can have. No mechanistic research. On the other hand, for smoking,
there are solid evidence: if y... 阅读全帖 |
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D******9
发帖数: 2665 | 30 no you don't have to. actually mouse and human breast structures are very
different. you can not mimic human breast environment in mouse. |
|
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w******w
发帖数: 32 | 32 大家从哪里看到实验室不错了，难道是因为赏了一口饭吃？
这个博后的工资，小硕毕业随便找个工作也能拿到，餐馆洗碗工也不会比这个少。
我辛辛苦苦周末加班没有休息日，熬更守夜看文献自己想idea，那还不是想做出个结果
，发篇文章，谋个前程？遇到难题担的只有自己，轮到出成果的时候，就找个不相关的
人来捡现成，把我顶开。这样子大家居然觉得是正常的？那什么是正常的科学伦理观？
还有的人说压两年，不管压多久，如果没有横插一杠这样的事情，我根本不会抱怨。如
果外面有同样的课题组发了，完全独立的，那损失是我的也是老板的，我没有怨言；但
是现在的状况是，我已经做完，另一个人是典型的喜欢抢发，但老板让它在我就自己的
工作做过无数次lab meeting的情况下来重复。做原创性的工作多难，mimic 一下有多
简单，大家应该都知道。老板不作为，损失就是我自己的。
我没有指望老板来写或者来帮忙发，但是发表的时候总得有他的同意啊；我也参加了些
meeting。但是这些都没有用。我纠结的是为什么他即使不能一碗水端平，是不是也要
保证我这个主要投入的工作没有如此的恶性竞争。 |
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z*t
发帖数: 863 | 33 Beutler得奖，Medzhitov会不会有意见？
http://www.sciencedirect.com/science/article/pii/S1074761309002
Ruslan Medzhitov
This year marks the 20th anniversary of a publication (Janeway, 1989) that
in many ways revolutionized our understanding of the immune system. As part
of the proceedings of the Cold Spring Harbor Symposium on Immune Recognition
, the paper authored by the late Charles A. Janeway, Jr. was not a standard
peer-reviewed publication. In fact, Charlie used to refer to it as “the
best paper I've ... 阅读全帖 |
|
|
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M**a
发帖数: 4816 | 36 26 January, 2012 Volume 1, Issue 1EditorialMore than the Sum of Its Parts p1
Boyana Konforti
Full Text | PDF (62 kb)
More than the Sum of Its Parts
Boyana Konforti
Editor, Cell Reports
Welcome to the first issue of Cell Reports, a new open-access journal that
covers all of biology with a focus on high-quality short papers. There are,
of course, other open-access journals—in fact, quite a number have launched
just in recent years (and there will almost certainly be more to follow)—
though few hav... 阅读全帖 |
|
M**a
发帖数: 4816 | 37 26 January, 2012 Volume 1, Issue 1EditorialMore than the Sum of Its Parts p1
Boyana Konforti
Full Text | PDF (62 kb)
More than the Sum of Its Parts
Boyana Konforti
Editor, Cell Reports
Welcome to the first issue of Cell Reports, a new open-access journal that
covers all of biology with a focus on high-quality short papers. There are,
of course, other open-access journals—in fact, quite a number have launched
just in recent years (and there will almost certainly be more to follow)—
though few hav... 阅读全帖 |
|
a*****x
发帖数: 901 | 38 可以做一些简单的caspase activity或者cell calcium之类简单的assays，如果有变化
也就提供了部门机制。同时可以从另一个方向看这个蛋白敲除是否mimic某种疾病表型
，然后这种疾病中是否有很多这个疾病的突变。 总之要么弄一点phenotype （in vivo
level的），要么弄一点mechanism，这样投CNS希望大一些，reviewers喜欢问这些问
题。 |
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a*****x
发帖数: 901 | 39 Just my two cents, not in the field...
1) pulse chase (fluore or isotope) or CHX treatment or SNAP tag with or
without over-expression of E (or knock-down)
2) Phospho versus dephospho mimic mutation; If the sites are not clear, try
to look for phospho change by over-expression of E (or knock-down)
3) specific inhibitors |
|
b*****n
发帖数: 1841 | 40 果然
大快人心！
RETRACTED: Enhanced Polyubiquitination of Shank3 and NMDA Receptor in a
Mouse Model of Autism
M Ali Bangash1, Joo Min Park1, 6, Tatiana Melnikova2, 6, Dehua Wang4, 6, Soo
Kyeong Jeon1, Deidre Lee2, Sbaa Syeda2, Juno Kim1, Mehreen Kouser3, Joshua
Schwartz1, Yiyuan Cui4, Xia Zhao4, Haley E. Speed3, Sara E. Kee3, Jian Cheng
Tu1, Jia-Hua Hu1, Ronald S. Petralia5, David J. Linden1, Craig M. Powell3,
Alena Savonenko2, Bo Xiao1, 4 and Paul F. Worley1, ,
1 Department of Neuroscience, Johns Hop... 阅读全帖 |
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l****e
发帖数: 157 | 41 for in vivo or in vitro studies? |
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f*****Y
发帖数: 20 | 42 What is the major difference of using such a mutant in in vivo or in vitro
studies?
I hope it can be used for both.
Thank you both for the reply. |
|
b******y
发帖数: 627 | 43 If the phosphorylation event is for disruption of an otherwise interactions,
YtoE works. However, if the phosphorylation event is to recruit a SH2 or
PTB containing binding partner, most likely this substitution will not work.
Of course, there are other scenarios this should or should not work.
good luck. |
|
l**********1
发帖数: 5204 | 44 Here you go
Deramaudt TB et al. (2011)
FAK phosphorylation at Tyr-925 regulates cross-talk between focal adhesion
turnover and cell protrusion.
Mol Biol Cell. 22: 964-75.
Abstract
Cell migration is a highly complex process that requires the coordinated
formation of membrane protrusion and focal adhesions (FAs). Focal adhesion
kinase (FAK), a major signaling component of FAs, is involved in the
disassembly process of FAs through phosphorylation and dephosphorylation of
its tyrosine residues, but ... 阅读全帖 |
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s******y
发帖数: 28562 | 45 http://news.sciencemag.org/scienceinsider/2012/08/amgen-pulls-c
Amgen 公司试图开发的单抗药物宣布失败。该药品是抗 type 1 insulin-like growth
factor receptor，在细胞和动物模型中有理想的效果。而且，在动物
和细胞模型里面过表达IGF-1会导致癌细胞行为并能被该抗体抑制。
但是在phase III clinical trial中得到的数据表明该药物对病人身上的
多种癌症都没有疗效。
其他类似的单抗药物目前看来效果也不理想，研究者们认为大部分癌症（尤其是固体瘤
）的机理比较复杂，用单一靶点可能是很难抑制癌症的。而且在初期研究中使用的癌症
模型也不能被视为是真正的癌症例子。
另外几个公司目前在试图把 IGF-1R 和 insulin receptor一起阻断，看看能不能得到
更好的效果。
Amgen Pulls Cancer Drug
by Jennifer Couzin-Frankel on 10 August 2012, 3:50 PM | 2 Comments
Share on... 阅读全帖 |
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l**********1
发帖数: 5204 | 46 laodaya mimic id came already,
escape now, martix. |
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s******y
发帖数: 28562 | 47 有啊。
Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):11259-63.
Signal-induced degradation of I kappa B alpha requires site-specific
ubiquitination.
Scherer DC, Brockman JA, Chen Z, Maniatis T, Ballard DW.
Source
The inhibitor protein I kappa B alpha controls the nuclear import of the
transcription factor NF-kappa B. The inhibitory activity of I kappa B alpha
is regulated from the cytoplasmic compartment by signal-induced proteolysis.
Previous studies have shown that signal-dependent phosphorylation ... 阅读全帖 |
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c********b
发帖数: 363 | 48 应该不会，immidazole是histidine的mimic而已，而不是象高盐高detergent那样。
exchange |
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t*d
发帖数: 1290 | 49 IMHO, to find novel drug delivering methods might be better ways to conquer
cancer comparing to studying the mechanisms of cancer initiation and
progression.
Nature 491, S58–S60 (22 November 2012)
Nanotechnology: Carrying drugs
When Joseph DeSimone makes nanomedicines, he compares himself to a baker. He
mixes drugs with different chemical 'batters', puts them in tiny moulds,
cures them and then turns them out. He can mould almost any shape: discs,
cubes, long sticks, roughened doughn... 阅读全帖 |
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h****y
发帖数: 77 | 50 By GINA KOLATA
For decades, mice have been the species of choice in the study of human
diseases. But now, researchers report evidence that the mouse model has been
totally misleading for at least three major killers — sepsis, burns and
trauma. As a result, years and billions of dollars have been wasted
following false leads, they say.
The study’s findings do not mean that mice are useless models for all human
diseases. But, its authors said, they do raise troubling questions about
diseases like ... 阅读全帖 |
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