p****l
发帖数: 291 | |
a****d
发帖数: 1919 | 2 My two points of view:
Most organ development happened along with angiogenesis. Within developing
tissue, the multi-potent cells/progenitors are normally close to vasculature
, while those more differentiated cells are away from the vessles.The
developing tissue always secreted angiogenesis cues like VEGF, and the
growing vasculature helped to further pattern the tissue in a feedback way.
As to your question, normal organ development should be impaired in hypoxia
environment.But during normal organ development, different sub population of
cells might expose to different level of oxygen for sure. |
p****l
发帖数: 291 | 3 干细胞快速增殖分化的时候会有类似癌症的状态吗？
或者干细胞本身和癌细胞有类似处吗？
我好像看过一篇cell paper，不过现在记不清楚了
vasculature
.
hypoxia
of
【在 a****d 的大作中提到】
: My two points of view:
: Most organ development happened along with angiogenesis. Within developing
: tissue, the multi-potent cells/progenitors are normally close to vasculature
: , while those more differentiated cells are away from the vessles.The
: developing tissue always secreted angiogenesis cues like VEGF, and the
: growing vasculature helped to further pattern the tissue in a feedback way.
: As to your question, normal organ development should be impaired in hypoxia
: environment.But during normal organ development, different sub population of
: cells might expose to different level of oxygen for sure.
|
a****d
发帖数: 1919 | 4 Those are very general questions.
For stem cells/progenitors, self-renewal and proliferation are two distinct
events, although many people don't separate these two when talking about
behavior of stem cells/progenitors. If you ask about proliferation of stem
cells, it seems pretty much similar pathways also regulate tumorgenesis. To
be more specific, differentiation of stem cells/progenitors also mimics EMT,
at certain level. Angiogenesis is ongoing along with progenitors
proliferation, same as tumorgenesis.
For ur second question, people study cancer stem cells could give you a
better answer. To me, all different types of cell are similar, at certain
level. In vivo environment has much strong instructive role for cell fate
maintenance or switch.
【在 p****l 的大作中提到】
: 干细胞快速增殖分化的时候会有类似癌症的状态吗？
: 或者干细胞本身和癌细胞有类似处吗？
: 我好像看过一篇cell paper，不过现在记不清楚了
:
: vasculature
: .
: hypoxia
: of
|
w***a
发帖数: 4361 | 5 糖尿病field，有一种说法是adipose tissue在快速增殖的时候，angiogenesis跟不上
obesity的expansion，造成local hypoxia。而hypoxia引起inflammation，
然后recruit macrophage，inflammation继续加重。
这种恶性循环最后导致global insulin resistance 。
【在 p****l 的大作中提到】
: rt
|
p****l
发帖数: 291 | 6 thanks a lot, this is exactly the answer I want.
and could u please give me the papers supporting this theory? I just wan
t to know which diabetes model they used, and how they showed the
relationship between hypoxia and inflammation.
【在 w***a 的大作中提到】
: 糖尿病field，有一种说法是adipose tissue在快速增殖的时候，angiogenesis跟不上
: obesity的expansion，造成local hypoxia。而hypoxia引起inflammation，
: 然后recruit macrophage，inflammation继续加重。
: 这种恶性循环最后导致global insulin resistance 。
|
p****l
发帖数: 291 | 7 really thanks.
distinct
To
EMT,
【在 a****d 的大作中提到】
: Those are very general questions.
: For stem cells/progenitors, self-renewal and proliferation are two distinct
: events, although many people don't separate these two when talking about
: behavior of stem cells/progenitors. If you ask about proliferation of stem
: cells, it seems pretty much similar pathways also regulate tumorgenesis. To
: be more specific, differentiation of stem cells/progenitors also mimics EMT,
: at certain level. Angiogenesis is ongoing along with progenitors
: proliferation, same as tumorgenesis.
: For ur second question, people study cancer stem cells could give you a
: better answer. To me, all different types of cell are similar, at certain
|
b*****l
发帖数: 9499 | 8 能说说 differentiation 和 EMT 的相似之处么？。。。
一直觉得 stem cell 更 M，而 differentiated cells 更 E 啊。。。
distinct
To
EMT,
【在 a****d 的大作中提到】
: Those are very general questions.
: For stem cells/progenitors, self-renewal and proliferation are two distinct
: events, although many people don't separate these two when talking about
: behavior of stem cells/progenitors. If you ask about proliferation of stem
: cells, it seems pretty much similar pathways also regulate tumorgenesis. To
: be more specific, differentiation of stem cells/progenitors also mimics EMT,
: at certain level. Angiogenesis is ongoing along with progenitors
: proliferation, same as tumorgenesis.
: For ur second question, people study cancer stem cells could give you a
: better answer. To me, all different types of cell are similar, at certain
|
w***a
发帖数: 4361 | 9 这里有篇review， PMID: 19698203， 类似的paper还有很多。
不过这个理论也就是个hypothesis，在Obesity领域还没有被广大学霸广泛认可。
【在 p****l 的大作中提到】
: thanks a lot, this is exactly the answer I want.
: and could u please give me the papers supporting this theory? I just wan
: t to know which diabetes model they used, and how they showed the
: relationship between hypoxia and inflammation.
|
M*****n
发帖数: 16729 | 10 EMT? 俺觉得ＥＭＴ应该是ｄｅｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ．
【在 b*****l 的大作中提到】
: 能说说 differentiation 和 EMT 的相似之处么？。。。
: 一直觉得 stem cell 更 M，而 differentiated cells 更 E 啊。。。
:
: distinct
: To
: EMT,
|
|
|
p****l
发帖数: 291 | 11 cellular hypoxia? 啥意思？
这个abstract我看到的几个关键词和我的想法很吻合
也许做自身免疫的应该参与进来，haha
【在 w***a 的大作中提到】
: 这里有篇review， PMID: 19698203， 类似的paper还有很多。
: 不过这个理论也就是个hypothesis，在Obesity领域还没有被广大学霸广泛认可。
|
p****l
发帖数: 291 | 12 epithelial cells和mesenchymal cells的划分很明显吗？
【在 M*****n 的大作中提到】
: EMT? 俺觉得ＥＭＴ应该是ｄｅｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ．
|
p****l
发帖数: 291 | 13 其实我不很清楚大家怎么定义tumor的微环境，但是器官发育里面那些正在分化的
stromal cells就是微环境最重要的组成
器官发育的时候很多血管应该还没有完全形成，为什么在正常器官里构成是有序的，但
是癌症里不是？有推荐的文献没？
谢谢
distinct
To
EMT,
【在 a****d 的大作中提到】
: Those are very general questions.
: For stem cells/progenitors, self-renewal and proliferation are two distinct
: events, although many people don't separate these two when talking about
: behavior of stem cells/progenitors. If you ask about proliferation of stem
: cells, it seems pretty much similar pathways also regulate tumorgenesis. To
: be more specific, differentiation of stem cells/progenitors also mimics EMT,
: at certain level. Angiogenesis is ongoing along with progenitors
: proliferation, same as tumorgenesis.
: For ur second question, people study cancer stem cells could give you a
: better answer. To me, all different types of cell are similar, at certain
|
M*****n
发帖数: 16729 | 14 比如说polarity 还有epithelial marker etc.
这个所谓的EMT不好说，好像有人argue未必非的经过这个EMT阶段的。
【在 p****l 的大作中提到】
: epithelial cells和mesenchymal cells的划分很明显吗？
|
a****d
发帖数: 1919 | 15 I am not working on EMT,so don't take my words too seriously.
In adulthood, within certain organs, stem cells/progenitors proliferate and
differentiate to maintain homeostasis. It looks like what happened during
this homeostasis maintenance, are utilizing similar group of EMT related
genes.I am not saying the stem cells behave like "M",or the differentiated
cells like"E".I just thought similar groups of genes were involved in
homeostasis.
Morphin said EMT was de-differentiation, and I do agree on that.
【在 b*****l 的大作中提到】
: 能说说 differentiation 和 EMT 的相似之处么？。。。
: 一直觉得 stem cell 更 M，而 differentiated cells 更 E 啊。。。
:
: distinct
: To
: EMT,
|
b*****l
发帖数: 9499 | 16 yep, that is what i think...
【在 M*****n 的大作中提到】
: EMT? 俺觉得ＥＭＴ应该是ｄｅｄｉｆｆｅｒｅｎｔｉａｔｉｏｎ．
|
b*****l
发帖数: 9499 | 17 ok, so it's EMT-related gene, not EMT.
and
【在 a****d 的大作中提到】
: I am not working on EMT,so don't take my words too seriously.
: In adulthood, within certain organs, stem cells/progenitors proliferate and
: differentiate to maintain homeostasis. It looks like what happened during
: this homeostasis maintenance, are utilizing similar group of EMT related
: genes.I am not saying the stem cells behave like "M",or the differentiated
: cells like"E".I just thought similar groups of genes were involved in
: homeostasis.
: Morphin said EMT was de-differentiation, and I do agree on that.
:
|
w******a
发帖数: 1527 | 18 For iPS cells, blocking the p53 pathway can improve the efficiency of
transforming differentiated cells into induced pluripotent stem cells.
So some people suggested that iPS cells are just like the tumor cells.
【在 p****l 的大作中提到】
: 干细胞快速增殖分化的时候会有类似癌症的状态吗？
: 或者干细胞本身和癌细胞有类似处吗？
: 我好像看过一篇cell paper，不过现在记不清楚了
:
: vasculature
: .
: hypoxia
: of
|
w******a
发帖数: 1527 | 19 I think immune cells such macrophages also are a part of the tumor
microenvironment.
【在 p****l 的大作中提到】
: 其实我不很清楚大家怎么定义tumor的微环境，但是器官发育里面那些正在分化的
: stromal cells就是微环境最重要的组成
: 器官发育的时候很多血管应该还没有完全形成，为什么在正常器官里构成是有序的，但
: 是癌症里不是？有推荐的文献没？
: 谢谢
:
: distinct
: To
: EMT,
|
w******a
发帖数: 1527 | 20 I remembered some paper saying some type of tumor will undergo MET (
mesenchymal to epithelial transition), if I understand correctly.
And I have been wondering whether the instructive signals for EMT in tumors
are the same as that in normal development...
【在 M*****n 的大作中提到】
: 比如说polarity 还有epithelial marker etc.
: 这个所谓的EMT不好说，好像有人argue未必非的经过这个EMT阶段的。
|
|
|
b*****l
发帖数: 9499 | 21 more details pls?
tumors
【在 w******a 的大作中提到】
: I remembered some paper saying some type of tumor will undergo MET (
: mesenchymal to epithelial transition), if I understand correctly.
: And I have been wondering whether the instructive signals for EMT in tumors
: are the same as that in normal development...
|
w******a
发帖数: 1527 | 22 did you ask the MET?
epithelial reverting transition>
epithelial reverting transition in leiomyosarcoma through regulation of slug>
...
【在 b*****l 的大作中提到】
: more details pls?
:
: tumors
|
b*****l
发帖数: 9499 | 23 赞
to
slug>
【在 w******a 的大作中提到】
: did you ask the MET?
: : epithelial reverting transition>
: : epithelial reverting transition in leiomyosarcoma through regulation of slug>
: ...
|
w******a
发帖数: 1527 | 24 I didn't read the paper, sorry, but I am curious that whether this
inflammaotory response will play a positive role in this process.
I'm asking because some injury indeed will attract the immune cells to the
site but the roles of inflammaotory cells can't be simply regarded as "good"
or "bad"...
【在 w***a 的大作中提到】
: 糖尿病field，有一种说法是adipose tissue在快速增殖的时候，angiogenesis跟不上
: obesity的expansion，造成local hypoxia。而hypoxia引起inflammation，
: 然后recruit macrophage，inflammation继续加重。
: 这种恶性循环最后导致global insulin resistance 。
|
p****l
发帖数: 291 | 25 I like those bad cells.
good"
【在 w******a 的大作中提到】
: I didn't read the paper, sorry, but I am curious that whether this
: inflammaotory response will play a positive role in this process.
: I'm asking because some injury indeed will attract the immune cells to the
: site but the roles of inflammaotory cells can't be simply regarded as "good"
: or "bad"...
|
w******a
发帖数: 1527 | 26 They are not 100% bad...
【在 p****l 的大作中提到】
: I like those bad cells.
:
: good"
|
