m****s
发帖数: 18160 | 1 【 以下文字转载自 Postdoc 讨论区 】
发信人: fsypl (mimi), 信区: Postdoc
标 题: PostDoc Position polymer membranes KY
发信站: BBS 未名空间站 (Mon Oct 22 14:41:35 2012, 美东)
Postdoctoral Scholar
The University of Kentucky, Center for Applied Energy Research (CAER) is
seeking to fill an immediate postdoctoral opening. The
successful candidate will develop polymer membrane for gas/liquid separation
. The position requires a Ph.D. in materials science,
membrane separation science, chemistry, chemical engineering, polymer or a
... 阅读全帖 |
|
f***l
发帖数: 22 | 2 http://www.caer.uky.edu/powergen/news/Membrane_PostDoc_2012.pdf
Postdoctoral Scholar
The University of Kentucky, Center for Applied Energy Research (CAER) is
seeking to fill an immediate postdoctoral opening. The
successful candidate will develop polymer membrane for gas/liquid separation
. The position requires a Ph.D. in materials science,
membrane separation science, chemistry, chemical engineering, polymer or a
related field from an accredited college or university.
Prior experience in any f... 阅读全帖 |
|
c**********a
发帖数: 6 | 3 Effective application of dendrimers in the field of ultra-low pressure
reverse osmosis membrane.
Dendrimer-based ultra-low pressure reverse osmosis membrane can effectively
retain a variety of salts, such as sodium sulfate, sodium chloride,
magnesium sulfate, retention rate reaches 92%, in particular sodium sulphate
, up to 98%. The membrane has high flux, and low operating pressure. Water
flux can reach 30-40 L • M-2 • h - 1, operating pressure was 0.
6MPa. The film has good resista... 阅读全帖 |
|
a*****t
发帖数: 81 | 4
200 mv ? is it possible ?
Normally, rest membrane potential of neurons is about minus 50 to 80 mv,
as the outside membrane be the reference potential. For some astrocytes,
the value is about minus 20 to 30 mv. I can not recall the value for muscle
cells, but it is also minus.
You are right that you can assume the potential is distributed on the
membrane(double layer). Because compared with the resistance and capacitance
of the membrane, intra- and extracellular contributions c |
|
g**********r
发帖数: 422 | 5 Development of a PDMS-grafted alumina membrane and its evaluation as solvent
resistant nanofiltration membrane"
Journal of Membrane Science
请把简历发到k******[email protected] |
|
j*******d
发帖数: 54 | 6 If you can find the book written by Hille B. or any medical physiological
book, you will find the answer. According to the Nernst Equation (Nernst
1888), Ek=RT/ZF*ln[K]o/[K]i, an increase in the extracellular K+ concentration
will cause less negative membrane potential (depolarization)! Remember at the
resting conditions, most cell membrane only permeates K+ through membrane, not
Cl-, Ca2+ or Na+. |
|
w******y
发帖数: 2504 | 7 The PVDF WB membrane was first probed with several different antibodies and
stripped between each probing, then it was stored in TBST. Yesterday, I
tried to probe for another target protein, seems like this old membrane dose
not work any more even if I probed with the antibody that worked on this
membrane. Any suggestions for this situation? Thanks. |
|
a****r
发帖数: 4965 | 8 Membrane asymmetry
1. Different composition inside and out:
It is important when thinking about membranes to keep in mind that a typical
cell membrane tends to have a different composition on one side (a.k.a.,
leaflet; say, the inside, or inner leaflet) than on the other (the outside, or
outer leaflet).
Differences tend to include different ratios or types of amphipathic
lipid-based molecules found in each leaflet, different kinds of proteins
facing in or facing out, or fixed orientations of pro |
|
n*****r
发帖数: 159 | 9 【 以下文字转载自 ChemEng 讨论区 】
发信人: njupeer (np), 信区: ChemEng
标 题: 寻找 Thin-Film Composite (TFC) Reserve Osmosis membrane 生产技术和合作伙伴
发信站: BBS 未名空间站 (Wed Jan 19 21:10:35 2011, 美东)
请问这里有研究Thin-Film Composite（TFC) Reserve Osmosis membrane 生产技术的
同胞，或者所在公司生产TFC RO membrane的吗？
我在帮一个中国公司寻找愿意转让或者合作生产TFC RO 净水膜的厂家或者研究单位。
如有相关信息，请站内联系，不甚感激！ |
|
n*****r
发帖数: 159 | 10 【 以下文字转载自 ChemEng 讨论区 】
发信人: njupeer (np), 信区: ChemEng
标 题: 寻找 Thin-Film Composite (TFC) Reserve Osmosis membrane 生产技术和合作伙伴
发信站: BBS 未名空间站 (Wed Jan 19 21:10:35 2011, 美东)
请问这里有研究Thin-Film Composite（TFC) Reserve Osmosis membrane 生产技术的
同胞，或者所在公司生产TFC RO membrane的吗？
我在帮一个中国公司寻找愿意转让或者合作生产TFC RO 净水膜的厂家或者研究单位。
如有相关信息，请站内联系，不甚感激！ |
|
k******u
发帖数: 1467 | 11 请问这个有必要加吗？basement的upgrade Foundation Membrane System
这是一个防水系统吗？不加会容易漏水吗？大概是2400多刀，新房不加，以后还可以加
吗？
Includes membrane system for entire Basement perimeter, from footing level
to finish grade. |
|
s********y
发帖数: 4047 | 12 虽然我还不到due date，但是医生希望我这个周末能生，说是害怕再等一个星期宝宝会
太大然后生不出来剖。让我星期五去她office做strip membrane。。她让我跟家里人商
量一下，我lg也是一头雾水。。strip membrane很疼吗？做完有帮助吗？还是从现在开
始我每天狂趴楼梯吃菠萝然后虐待lg呢？ |
|
n****8
发帖数: 4141 | 13 可以参考下这篇文章http://birthsen.tmdhosting930.com/?p=1661
“A while back, I took care of Annie (not her real name) during her labor.
Annie was a first-timer. She was five days past her due date, and one of my
colleagues decided to strip Annie’s membranes. Annie’s cervix was not
favorable in the least. It was only one centimeter dilated, thick, and felt
like the tip of your nose–pretty firm. It was very painful to strip the
membranes, as at one centimeter, the cervix is not open enough to admit the
... 阅读全帖 |
|
l*********1
发帖数: 940 | 14 简单说，就是OB内检时候用手指划拉一圈。。。。
Stripping the membranes is done carefully and gently in a pelvic exam, by a
care provider who sweeps a gloved finger around the cervix to separate the
amniotic membrane from the lower uterine wall. This does not equate to "
breaking the waters," though in some cases that can occur. This technique is
thought to release prostaglandins, which bring about contractions and
soften the cervix, according to the American Congress of Obstetricians and
Gynecologists. Labor may comme... 阅读全帖 |
|
l**********g
发帖数: 364 | 15 今天是预产期， 医生检查开了两指， 就给我做了strip the membrane，告诉我说如果
起作用，24小时之内就会进入labor的. 有经验的姐妹说说，到底这个strip the
membrane会多有效啊。 |
|
s******y
发帖数: 28562 | 16 I suggest you don't bother.
It is likely all the protein on the surface have been degraded due to
bacterial activity and other factors.
Well, however, if you are really, really,really desperate, you can try this:
wet the membrane in methanol for 5 minutes, then wash by PBS three times.
Then maybe you can try again.
Remember: if you want to keep a membrane for a long time, always dry it, and
then store at -20oC
and
dose |
|
c*******4
发帖数: 41 | 17 Does anyone know how to measure the concentration of protein that is bound
to lipid membrane? I can not measure it by UV/Vis because the phospholipid
greatly interferences the UV/Vis absorbance at very wide range.
I found a paper that used densitometry with a software (ImageQuant) to
determine the membrane bound protein concentration. Does anyone know how
reliable it would be. Many thanks!!! |
|
m**********2
发帖数: 57 | 18 想验证一个功能是基于beta-catenin membrane association 而不是Wnt signaling.
knockout beta-catenin 已得到类似结果,现在想disrupt beta-catenin membrane
association来验证是否有相同的结果.
现在想找个inhibitor,只是破坏beta-catenin膜结合而不影响Wnt signaling.
请问有谁知道么? |
|
f*******0
发帖数: 62 | 19 【 以下文字转载自 Faculty 讨论区 】
发信人: fishman80 (最爱鱼儿), 信区: Faculty
标 题: 请教几个用来产生 membrane proteins设备的价格
发信站: BBS 未名空间站 (Mon Feb 25 13:44:06 2013, 美东)
有幸得到一个AP position， 现需要估算个 startup package。 因为我主要是用E.
Coli express and purify membrane proteins，特向有经验前辈们请教几个主要用到
的仪器大概价格 （有个区间就行）。多谢多谢！
1. High performance liquid chromatography
2. 1L bacteria incubation shaker
3. OD spectrometer (for bacteria concentration measurement)
4. Allegra type centrifuge (for protein solution centrifuge／concentrate)
5. Bacteria ce... 阅读全帖 |
|
s*****3
发帖数: 41 | 20 Colloids Surf B Biointerfaces. 2014 Jan 1;113:176-81. doi: 10.1016/j.
colsurfb.2013.09.007. Epub 2013 Sep 12.
Membrane surface functionalization via theophylline derivative coating and
streptavidin immobilization.
http://www.sciencedirect.com/science/article/pii/S0927776513005
另外，有人用 streptavidin包被 PVDF平板来做elispot么 ？ streptavidin能很好地
coat PVDF membrane么 ？
谢谢 |
|
n*****r
发帖数: 159 | 21 请问这里有研究Thin-Film Composite（TFC) Reserve Osmosis membrane 生产技术的
同胞，或者所在公司生产TFC RO membrane的吗？
我在帮一个中国公司寻找愿意转让或者合作生产TFC RO 净水膜的厂家或者研究单位。
如有相关信息，请站内联系，不甚感激！ |
|
t*********t
发帖数: 1058 | 22 这些是MBR的主要制造商：
Asahi Kasei Group, GE Water & Process Technologies, Keppel Seghers Belgium
NV, Koch Membrane Systems, Inc., Kubota Corporation, Mitsubishi Rayon Co.,
Ltd., Norit NV, Pall Corporation, Siemens Water Technologies, Toray
Industries, Inc., and Veolia Environnement.
（最主要的生产厂商是 GE, Kubota, Siemens and Pall.)
有很多因素决定着那个厂商的mbr是最佳选择，譬如 cost, membrane life and
reliability, available area, utility consumption, wastewater discharge
quality standard, wastewater reuse 等等。
有篇文章有各个生产厂商的膜的性能参数的具体比较 |
|
发帖数: 1 | 23 是我自己顿悟出来的，说光子成亿万的弥散在大气空中，受风，温度的影响，有些地方
稀薄，有些地方浓度大。许多的薄厚之间产生许多的 semi transparent membrane
effect, 类似生理学上的半膜效应，会自体释散均匀。其实并没有实体膜，是浓度差和
电差造成的类似膜。 |
|
f*******0
发帖数: 62 | 24 有幸得到一个AP position， 现需要估算个 startup package。 因为我主要是用E.
Coli express and purify membrane proteins，特向有经验前辈们请教几个主要用到
的仪器大概价格 （有个区间就行）。多谢多谢！
1. High performance liquid chromatography
2. 1L bacteria incubation shaker
3. OD spectrometer (for bacteria concentration measurement)
4. Allegra type centrifuge (for protein solution centrifuge／concentrate)
5. Bacteria cell pelleting, harvesting centrifuge
6. ultracentrifuge
7. Benchtop microcentrifuge
8. mechanical bacterial cell breaker
9. UV absorption spectr... 阅读全帖 |
|
|
w**********n
发帖数: 257 | 26 请教各位JM，今天39W1天，检查开1cm，OB给我做了strip membrane和cervix massage
，一般这种情况，几天后宝宝可以发动啦？急着让宝宝出来。。。 |
|
|
K****n
发帖数: 756 | 28 看到membrane里会进去一些奶，奶常温下几小时就会有细菌，不洗不放心，但是每次都
拆开、装上觉得那个塑料的小盖子的搭扣很快就会坏了，大家是怎么弄的？谢谢！ |
|
h*****d
发帖数: 53 | 29 天，membrane和喇叭是肯定要每次用完都洗的...
我还在想，那根塑料的管子怎么洗，好像用了几次就会有奶星进去.... |
|
l*********1
发帖数: 940 | 30 35W时候大B超+内检，开1指，之后2次内检，每次都是 开1指+80%软化。
OB说建议做Stripping membranes。 看我们不懂啥意思，就写下来让我们回去想想，
下周去产检的时候就可以做了（38W）.
OB说做了这个有30%的可能性会在之后的24小时内生。
请问有没有JM做过这个？效果怎样啊？怕做了以后没有效果被拉去剖了咋办啊。。。
谢谢大家！ |
|
d*****1
发帖数: 566 | 31 这个催产是有概率的，做了不一定有用，我觉得有用的那些说不定也会自己发动。我同
事overdue,前天40周产检刚刚做了，今天还是来上班了，没用，约了明天催。我同事第
一个overdue 8天之后催产的，这是第二胎，已经用了各种方法：针灸，每天爬楼梯、
喝一种什么茶，天天吃菠萝，这个stripping membrane.最后的结论是：娃不想出来，
你怎么折腾也没用，只能等着催了。我不是想打击搂住，只是觉得还是顺其自然，不用
太纠结，搞得自己很痛苦。催也没什么不好，很多人都催得挺顺利的。 |
|
c******y
发帖数: 547 | 32 Ideally, stripping membrane would speed up your labor, however, it's not
that effective as I heard or at least on me. I did it at 38w and wasn't in
labor until the day before my due date. Just do it, it's not painful at all
and won't increase your risk to get a C-section. 38wks is considered full-
term already. |
|
c*****t
发帖数: 625 | 33 刚刚37w＋5d产检归来，内诊检查cervix very soft, length is about 1.5cm long。
医生说下周再来的时候要stripe the membrane，这样利于cervix dilation，because
it will increase the natural prostaglandin（a hormone dialates cervix）
release from the cervix. and because of this, this center rarely got overdue
baby.
网上的解释：
http://answers.yahoo.com/question/index?qid=20100119072236AAQua
虽然她说的都懂，可是妇产里从来没有提到过这个，想想医生是不是为了防止overdue
而有点过激呢？再说我现在还没有看到mucus plug 掉呢，是不是有点揠苗助长的意思
呢？
做过这个的姐妹能上来说说吗？搞得我现在心里都没底了。。。 |
|
w****n
发帖数: 56 | 34 有没有人做过Membrane sweep？做过后有什么反应？对催生有作用吗？多久就生了？谢
谢了！ |
|
c*********3
发帖数: 1002 | 35 这个是什么？我生以前五六天的时候最后一次产检，好像是做了这个，就是内检的时候
用手指帮忙扩张一下宫颈口，顺便伸展一下membrane.当时就出了点血，不怎么疼。
不知道是不是有催产的作用，至少医生说有用。我周一产检，开两指60%软化。周五晚
上进医院，开五指90%软化，周六早上生的。
★ 发自iPhone App: ChineseWeb 7.8 |
|
w****n
发帖数: 56 | 36 我现在开了2指半，宫颈75%软化，做了membrane sweep，不知多久能发动，现在40周了
，大家有经验吗？ |
|
w*****a
发帖数: 156 | 37 Backed with multi-million dollar funding and multiple fortune 500 companies'
s support, we are seeking a highly motivated postdoc researcher to join in
our lab, to work on the nano-membrane materials for water filtration and
treatment.
Qualifications:
1. Recent Ph.D. in Materials/Mechanical/Chemical/Environmental Engineering
or any related field.
2. Strong experience in nano-materials for water filtration/treatment
applications.
3. Strong research capability, with outstanding publication records... 阅读全帖 |
|
d*******8
发帖数: 126 | 38 在美为了绿卡，没办法，review文章太少，急求Molecular modeling polymer physics
， Nanostructured materials by design，block copolymer membrane方向。
不然要卷铺盖滚蛋了，各位同胞，帮帮忙吧 |
|
t********r
发帖数: 97 | 39 Topic：Membrane Potential, GABAA Receptor and Hepatic Tumor Stem Cells.
Journal：American Journal of Physiology - Gastrointestinal and Liver
Physiology。
如有兴趣并且研究背景相关者请尽快站内邮件联系。
Please send me the following information:
your name, email address, institute, position, research interest, and
selected relevant publications
如有合适者我会推荐，但不保证能成功。
谢谢！ |
|
s*******2
发帖数: 151 | 40 我可以。
Name: Shen, Minqian
Email: [email protected]
/* */
phd in neuroscience from Miami University, and MD from china.
最近在做关于PDMS membrane，跟我们学校的engineer合作 |
|
f******n
发帖数: 640 | 41 关键词：
ABPBI membranes, high temperature, polyelectrolyte, fuel cells
有兴趣的朋友请站内，请附上一段已推荐人口吻的介绍
谢谢了 |
|
K**4
发帖数: 1015 | 42 These days I am very confused with membrane lipids. I know some lipids are
involved in regulating cell signaling by interacting with lipid-binding
domains, such as phosphatidylserine, PtdIns(4)P, PtdIns(3)P, PtdIns(4,5)P2,
PtdIns(3,5)P2, PtdIns(3,4,5)P3. Is it correct or I put some wrong lipid here?
Also how about phosphatidylcholine and phosphatidylethanolamine? Are they
also functional in that way? Or they are just component lipid in memebrane?
Many thanks for your help. |
|
k*******c
发帖数: 142 | 43 I blocked the membrane overnight at RT. Is it still good for me to continue? |
|
h****k
发帖数: 182 | 44 请问有什么好的方法或者实验可以检测membrane bound receptor 是monomer，dimer,
还是multimer？ |
|
S****r
发帖数: 982 | 45 Cysteine-trap is a regular approach to confirm dimer of membrane proteins.
dimer
No. SDS-PAGE, reducing or non-reducing environment, depending on the linkers
you chosen.
No, SDS-PAGE |
|
f**g
发帖数: 28 | 46 As titled, is there any chemical inhibit nuclear membrane formation?
Thanks a lot. |
|
m*********n
发帖数: 158 | 47 哪个兄弟姐妹给个protocol把，主要想看一个protein 在不同得condition 下得
cytosol 和plamsic membrane enrichment,
有好得protocol或是好得kit可以推荐一下把，
谢谢 |
|
h****6
发帖数: 229 | 48 You gel was probably very low percentage.
Can you use another dry NC membrane to detach the page remains? |
|
l**********1
发帖数: 5204 | 49 同意 买买提 actin No. one faculty sunny day's 回复
BTW sunnyday 对
SMARCA2 相关的up and down stream realrive control transcription factors 熟
悉不?
SWI/SNF related, matrix associated, actin dependent regulator of chromatin,
subfamily a, member 2
----
Re:
发信人: sunnyday (飞鱼. 发胖中.飞不动了), 信区: Biology
标 题: Re: 什么是membrane ruffle?
发信站: BBS 未名空间站 (Tue Jul 3 11:46:03 2012, 美东)
就是细胞膜底下堆着高高一堆actin 把膜都拱起来了。 |
|
s******y
发帖数: 28562 | 50 Leading edge 这个词是指细胞有方向地移动的时候才会有这个概念， 所以不能
直接这么套用，但是，大部分membrane ruffling 是在leading edge 上。
这种结构在正常和病态下都可能出现，就是细胞对外界反应比较激烈的时候的表现。 |
|
