y***i
发帖数: 36 | 1 Two PhD positions are available immediately – computation and simulation of
fuel cells
Two PhD studentship positions are available immediately at the Center of
Solid Oxide Fuel Cell and the Department of Mechanical Engineering at the
University of South Carolina. The research is in the area of computation
and simulation of fuel cells, such as computational fluid dynamics (CFD),
finite element analysis of structure (FEA), thermal-fluid computation.
The starting time: Summer or Fall of 2008
Req |
|
y***i
发帖数: 36 | 2 Two PhD positions are available immediately – computation and simulation of
fuel cells
Two PhD studentship positions are available immediately at the Center of
Solid Oxide Fuel Cell and the Department of Mechanical Engineering at the
University of South Carolina. The research is in the area of computation
and simulation of fuel cells, such as computational fluid dynamics (CFD),
finite element analysis of structure (FEA), thermal-fluid computation.
The starting time: Summer or Fall of 2008
Req |
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t****t
发帖数: 45 | 3 Job Title: Postdoctoral Position in Biomaterials, Stem Cells, and Tissue
Regeneration
Application Deadline: Open Until Filled
Job Description
Description:
The Department of Biomedical Engineering at the University of Minnesota
currently has an opening for a post-doctoral fellow to conduct studies in
the area of biomaterials and tissue regeneration. The project involves
materials engineering on the molecular level, scaffold fabrication, and stem
cells.
Qualifications:
Candidates must have a Ph.D.... 阅读全帖 |
|
t****t
发帖数: 45 | 4 Job Title: Postdoctoral Position in Biomaterials, Stem Cells, and Tissue
Regeneration
Application Deadline: Open Until Filled
Job Description
Description:
The Department of Biomedical Engineering at the University of Minnesota
currently has an opening for a post-doctoral fellow to conduct studies in
the area of biomaterials and tissue regeneration. The project involves
materials engineering on the molecular level, scaffold fabrication, and stem
cells.
Qualifications:
Candidates must have a Ph.D.... 阅读全帖 |
|
y***i
发帖数: 36 | 5 Two PhD positions are available immediately – computation and simulation of
fuel cells
Two PhD studentship positions are available immediately at the Center of
Solid Oxide Fuel Cell and the Department of Mechanical Engineering at the
University of South Carolina. The research is in the area of computation
and simulation of fuel cells, such as computational fluid dynamics (CFD),
finite element analysis of structure (FEA), thermal-fluid computation.
The starting time: Summer or Fall of 2008
Req |
|
t****t
发帖数: 45 | 6 Job Title: Postdoctoral Position in Biomaterials, Stem Cells, and Tissue
Regeneration
Application Deadline: Open Until Filled
Job Description
Description:
The Department of Biomedical Engineering at the University of Minnesota
currently has an opening for a post-doctoral fellow to conduct studies in
the area of biomaterials and tissue regeneration. The project involves
materials engineering on the molecular level, scaffold fabrication, and stem
cells.
Qualifications:
Candidates must have a Ph.D.... 阅读全帖 |
|
y***i
发帖数: 36 | 7 Two PhD positions are available immediately – computation and simulation of
fuel cells
Two PhD studentship positions are available immediately at the Center of
Solid Oxide Fuel Cell and the Department of Mechanical Engineering at the
University of South Carolina. The research is in the area of computation
and simulation of fuel cells, such as computational fluid dynamics (CFD),
finite element analysis of structure (FEA), thermal-fluid computation.
The starting time: Summer or Fall of 2008
Req |
|
t****t
发帖数: 45 | 8 Job Title: Postdoctoral Position in Biomaterials, Stem Cells, and Tissue
Regeneration
Application Deadline: Open Until Filled
Job Description
Description:
The Department of Biomedical Engineering at the University of Minnesota
currently has an opening for a post-doctoral fellow to conduct studies in
the area of biomaterials and tissue regeneration. The project involves
materials engineering on the molecular level, scaffold fabrication, and stem
cells.
Qualifications:
Candidates must have a Ph.D.... 阅读全帖 |
|
t****t
发帖数: 45 | 9 Job Title: Postdoctoral Position in Biomaterials, Stem Cells, and Tissue
Regeneration
Application Deadline: Open Until Filled
Job Description
Description:
The Department of Biomedical Engineering at the University of Minnesota
currently has an opening for a post-doctoral fellow to conduct studies in
the area of biomaterials and tissue regeneration. The project involves
materials engineering on the molecular level, scaffold fabrication, and stem
cells.
Qualifications:
Candidates must have a Ph.D.... 阅读全帖 |
|
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a******9
发帖数: 161 | 11 My understanding is touch function is integrated into LCD cell - so goes in-
cell touch. Right now most touch function is enabled on a separate touch
sensor between cover glass and LCD cell. |
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M****e
发帖数: 70 | 12 I have been reading recent publications of stem cell research
on Nature and Science. Such topic is pretty hot, even data
that could not replicate the previous published resulst was
accepted on such top class journals. refer to Science 298:361
and related publications from that issue.
The most important finding is turnover of the dogma "terminal
differentiation" that states the differentiation state for most
cells are unchangeable. However, the fact is that more and more
stem cells other than emb |
|
t**d
发帖数: 52 | 13 如果8小时还没有附着的话，细胞肯定死了
不知道你的floating cells多不多，占多大比例？如果数目很少的话western,ladder根本
无法解决问题
我目前也在做这方面的研究，我通常使用flow cytometer来检测subG1或caspase,
但也需要有一定数量的细胞，fibrobalst实际上很容易培养的，你研究中出现的现象我也
遇到过，认为很可能是trypsin处理过头了，如果附着细胞还有不少，你可以把浮游的去
掉，继续培养剩下的细胞。
请问如何鉴定这些floating的细胞仅仅是floating而没有dying，还是它们已经进入cell
， |
|
f******g
发帖数: 1003 | 14 I am working on extracting total RNA from few PFA-fixed cells (about 200
cells).
Now we have MagMAX™-96 Total RNA Isolation Kit (Cat:AM1830). This kit
is for RNA extraction from normal cells.
Did anyone have experience on it?
Thank you in advance, |
|
i***R
发帖数: 663 | 15 Yes new iGluR is selective Silencer because it is selective for K. They
took so much trouble to get the selectivity right, and it is a great
improvement.
If ChR2 is selective for Na but not for Ca or Mg it will be much better.
Ca do so much more than depolarization. If we want to ultimately
introduce the channel into the brain to stimulate some neuronal cell, it
is better it won't cause Ca overload.
TRPV1 like ChR2 is non-selective cation channel that causing pain
sensation, but long term activa... 阅读全帖 |
|
g*********1
发帖数: 459 | 16 我最近2-3年都在为Cell Research审稿。目前Cell research的审稿要求是提高了不少
。我在收到Cell Research 稿件时一般是把它定位在JBC这个档次。 |
|
n*q
发帖数: 258 | 17 Cell viabiliy: IC50 这个IC50是指什么。
SJSA-1 是衡量什么参数用的。这个数据是大好还是小好。
HCT116 是衡量什么参数用的。是大还是小比较好。
Other cell assay: EC50
p53RE-Luc，这个luciferase是衡量p53和luciferase的什么参数
Caspase 3/7，这个量是衡量什么用的。
hERG, (VC): 20% inhibition @ 6 uM这个是什么意思啊。
这个是一个p53抗癌药物的cell assay，从专利上看来的。不是学生物的，google了一
圈，也看不太明白。
求大牛给个比较简单的解释，或者给个网站，偶去好好科普一下。 |
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a****d
发帖数: 1919 | 18 http://www.nature.com/news/2010/100629/full/466017a.html
Stem-cell furore erupts
Data analysis ignites public row.
Alison Abbott
Long-rumbling hostilities between stem-cell researchers in Germany exploded
into a blazing public row last week, after Nature published a critical
reanalysis of data from a high-profile 2008 article.
The researchers behind the original work1, led by Thomas Skutella of the
University of Tübingen, reported using cells from adult human testes to
create pluripotent stem ce |
|
n********k
发帖数: 2818 | 19 layman on cancer stuff and no intent to argue but discuss...
First don't like Amy and have no knowledge about Kim before ur post...
that said, Most of the points you made here are points for discussions or
personal scientific standing, and more or less representing the challenging
for the entire cancer (or CSC) field and highly debatable...with that, your
strong opinion towards them kind of not justified...
I think we all have heard too much of pro and con concerning the various
theory regardi... 阅读全帖 |
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w**********9
发帖数: 242 | 20 俺觉得这个polyclonal origin 很山寨. 难以置信. 等大牛或教科书改了后俺跟着就是
了.
As of heterogeneity, is this you made up or you got from somewhere ? Did
you ever read Volgestin's work about tumorigenesis in spontaneous colon
cancer ?
=======================================================
3. heterogeneity
As for the heterogeneity of primary tumor, my understanding is that tumor st
arted from one cell that got original mutation and started to divide. When c
ell expanded, some of the cells accumulated other mutations. Alth... 阅读全帖 |
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r***e
发帖数: 2539 | 21 Yes, that's why I raised the question at the beginning. It seems that most o
f the cancer cell line can initiate tumor, but is there a sub-population in
these cell lines that acts like CSC?
As for what is important, in clinical? It's hard to tell. Say, cell origin o
f cancer is hot in basic field, but does this question help curing the cance
r in clinical? |
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a*****g
发帖数: 543 | 22 Jumping to a deep water hole...
I would caution the widely "credited" potential of CSC(technically here refe
ring to the tumor-inititating cells identified in immune-deficient mice) ass
ociated with relapse, drug resistance and metastasis.
Can anyone point out an original research paper with some experimental data
showing these cells have any of the three major capabilities? My impression
is sadly none...But we read it everywhere...
Second, CSCs are defined vaguely. The standards mentioned by ht... 阅读全帖 |
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m***e
发帖数: 16 | 23 想在神经细胞中表达一个蛋白，想制作一个stable cell line。
但是发现神经细胞都是原代培养的，
故想请教有什么neural cell line能持续分裂，并且可以用来做transient
transfection和
stable cell line呢？谢谢 |
|
h***3
发帖数: 26 | 24 i know this sounds extremely stupid...
I am new in biology
I did the E coli (SURE2) transformation in the cell culture hood... as i
remember I did not drop the bacteria cells out, just some LB media...
I spray the hood with 70% ethanol for many times, and turn the UV light for
10min,
is it possible for the bacteria cells to contaminate the laminar flow hood???
What should I do to de-contaminate the hood?
help me! I do not want to get this trouble...
Thanks a lot!! |
|
x******i
发帖数: 374 | 25 现在stem cell 热， neural stem cell 也热吗？
想进公司，不知机会多不多？
跟cancer and cell biology 相比呢？
请大家各抒己见，谢谢谢谢 |
|
l**********n
发帖数: 201 | 26 PLoS Biol 自己的标准是 CNS 级的，虽然很多时候也是 CNS 不中的文章转投过来。
Dev Cell 投稿也可以同时再投一个 Mol Cell 之类的 cell sister journal，也许中
奖率会高一点？至少他们的 policy 是允许怎么做的，不算 misconducts. |
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g*********d
发帖数: 233 | 27 人们都知道锻炼身体可以促进新陈代谢，有益于心血管健康，但此前科学家对运动究竟如何影响心脏
却所知甚少。美国哈佛大学医学院的研究人员日前报告说，他们首次从分子水平发现运动有益心脏健
康的机理，这一发现将有助于开发出治疗心血管疾病的新疗法。
研究人员通过小鼠实验发现，经常运动可以使小鼠体内的C/EBPβ转录因子水平显著下降，其结果会促
进小鼠心脏肌肉细胞增殖，有益于心脏生长。此外，研究人员还发现，体内C/EBPβ水平较低的小鼠对
心力衰竭具有抵抗能力。
研究人员表示，这项研究对心脏肌肉再生的潜力有了深入理解。参与研究的哈佛大学医学院教授安东
尼·罗森茨魏希表示，通过这项研究可以开发出针对那些无法运动的心脏病患者的疗法。
这项研究成果发表在新一期美国《细胞》杂志上。
C/EBPβ Controls Exercise-Induced Cardiac Growth and Protects against
Pathological Cardiac Remodeling
Cell, Volume 143, Issue 7, 1072-1083, 23 December 2010
10.1016/j... 阅读全帖 |
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s******s
发帖数: 13035 | 28 现在做的一个cell一用抗生素就碎掉了，本身又不太attach，所以
没法通过反复pbs洗来去除死细胞。我试了一个密度梯度离心histopaq，
有很多细胞被扔掉了，而且有点担心会不会弄死细胞。各位有什么高招？
我想用cell strainer把cell留下，碎片流掉。但是现在的strainer
多是40/70/100um的，有没有比较小的strainer或者mesh, 大概5－10um的？ |
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s****9
发帖数: 932 | 29 No perfect methods. It largely depends on your research purpose.
Cells carrying beads are bad for their trafficking in vivo. But magnetic beads enrichment is quick and relatively economic, as compared to sorting. For in vitro study like RNA purification or culture, beads purification is fine.
Sorting is expensive and labor-intensive (I suppose your lab do not have
your own sorter and do not sort cell frequently). But without beads binding
, cells are more healthy. |
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f**g
发帖数: 28 | 30 I transfected different oncogenes into 293 cells. I'm wondering whether
some oncogenes may affect cells and make them sensitive to freeze down. If
so, there must be some factors changed by the oncogene. Then this should a
part of function of oncogene.
What are factors associated with cell freezing? |
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l**********n
发帖数: 201 | 31 还是看人家网站上怎么说的吧？
Cosubmission
In the limited number of instances where a paper is potentially appropriate
in scope for the readership of two different Cell Press titles, the Cell
Press journals also offer authors a unique opportunity not provided by any
other journal group to submit a manuscript for joint consideration at two
journals at the same time. Authors interested in pursuing this option should
please contact the Editors of both journals for further information.
http://www.cell.com/authors |
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s**********e
发帖数: 2888 | 32 领域里面一个比较牛校的实验室，很多年的工作都是基于一个他们实验室才有的cell
line，他们没有描述这个cell line是怎么来的，只是一句话很笼统地说是从ATCC的细
胞subclone来的，引用文献是最早ATCC的那个细胞的文献。
我搜索了一下他们用的细胞的名字，所有的文献都是他们实验室的。给PI写了一封信，
问能否把cell寄给我。
但是我还是觉得，这样的工作，有什么意义？那个细胞又没有什么经济价值，到底是啥
不清不楚，发这种别人都没有办法重复的文章，有什么意义？ |
|
b******n
发帖数: 80 | 33 用什么蛋白区分hela cell和同组织内的正常cell
谢谢。
我是学电子的，顶钢盔通过。 |
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y****6
发帖数: 196 | 34 HEK293 cells in 96-well plate, DMEM with 10% FBS, cultured 2d, about 100%
confluency.
Washed once with PBS before adding fixation solution (3.7% formaldehyde/PBS).
Cells detached in fixastion solution. Is it because formaldehyde does not
work with HEK293 cells? Anyone tried methenol? What's the concentration? |
|
a****d
发帖数: 1919 | 35 I do use 45um filter to filter through the lysate before preparing the rest
procedures. I guess you can collect smaller aggregates less than certain
diameters.
The other thing is the purity of your cells. If the lysate is prepared from
heterogeneous population, where cells have different sizes, than you have to
be careful to isolate large cells and small aggregates. |
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W*******a
发帖数: 1769 | 36 你要tweak FACS才能收集aggregate，因为它default是要把所有不是single cell
event都排除掉的。此外cell aggregate形状不规则，你很难收集一个homogenous的
sample，会有很大的样本偏差。如果你规定只收集2 cell的，倒是还可能比较纯
sort? |
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w*e
发帖数: 740 | 37 lack of general interest is always an excuse to reject paper.
many papers on Nature Science Cell has no general interests but very
specific
to each field.
don't think too much of it. |
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v***a
发帖数: 1242 | 38 谢谢！MDCK代表Madin-Darby canine kidney，是一种epithelial cells。我不知道它
们作为cell line，是如何被immortalized的呢？
p53是那个可以让非cancer cells immortalized的因素吗？ |
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A******y
发帖数: 2041 | 39 There are a lot of immortalize techniques...for example the most common
human kidney cell line Hek293 is an embryonic kidney cell transformed with
adenovirus 5 DNA. Usually the immortalization is included in the cell line
information sheet.
http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/ta |
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E**********t
发帖数: 56 | 40 I chose these two papers for my journal club last year...
I didn't read them again, but in my memory, these two papers tried to
address the same question: why chemotherapy drug,tamoxifen, suppressed
breast cancer cell growth, but promotes endometrial tumorigenesis. Shang's
paper showed that tamoxifen could trans-activate PAX2 gene expression in
endometrial cancer cells, and the activation of PAX2 resulted in the
endometrial malignance.. The 2008 Nature paper showed that PAX2 is required
for tamo... 阅读全帖 |
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l******n
发帖数: 298 | 41 看见有circulation 发表文章说是用bone marrow derived mononuclear cells,还有说
是用bone marrow derived mesenchymal cells.
不知道有什么区别，请大家指教一下 |
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D***a
发帖数: 516 | 42 我有好几年没怎么看MSC的文章了，但好像这些年这领域也没什么突破性进展。我就说
几句，供参考。
直接用bone marrow MNC和分离培养MSC再注射的文章都有很多。直接用MNC的考虑大概
是骨髓中到底什么细胞是真正的能够transdifferentiation的stem cell没有公认的结
论，有人说是是在MSC群体里，有人说是是在HSC群体里。所以从应用的角度，直接用
MNC而不是分离纯化好的细胞，不会遗漏任何可能的stem cell。还有一点考虑，全骨髓
移植已经成功应用到临床有50多年了，其可能的风险和经验已经积累了很多，现在只是
将其用于新的疾病，便于被批准。而MSC之类的，需要经过体外培养，近十年才开始小
规模临床试验，风险和效果未知，推广起来挺难。当然，这并不妨碍实验研究，MNC和
MSC都可以发文章。
关于是否用辐射，辐射只能损伤快速增殖的组织，比如骨髓，肝脏，小肠粘膜等，对血
管平滑肌损伤效果未必好。很多组织特异性的损伤都有其自己的方法，比如心血管用缺
血再灌注，胰岛用STZ。我不清楚血管平滑肌是否有特异性的损伤方法，但放射损伤我
不认为它是一个很广泛的适应症。 |
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h*******o
发帖数: 4884 | 43 3-NPA does that. 3-NPA directly inhibits succinate dehydrogenase activity.
At sub-toxic doses cells are still viable, but SDH activity could be significantly inhibited.
MTT/MTS/Calcein Am/Resazurin assays are often over-used or somewhat used
incorrectly to measure cell viability/cell number.
your
seen |
|
h*******o
发帖数: 4884 | 44 As mentioned above, a lot of the cytotoxicity/cell viability assays
are based on mitochondrial function, particularly SDH activity. This renders
a lot of the assays under question when the "manipulation/treatment" have
potential impacts on mitochondrial function.
For cancer cell lines, it is less of an issue because of the
notorious "warburg" effect through which cancer cells are more glycolytic
and rely much less on mitochondrial OXPHOS.
But for primary cultures, like... 阅读全帖 |
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m**h
发帖数: 381 | 45 做了好几年的工作，virus infection and host immune system的领域，攒出来一大堆
date，故事也说圆了，老板也认为不错，觉得我得工作是目前关于这个问题最完整最有说服力的研究工作，可惜老板一直觉得新意不够。
刚投了immunity，审稿一个月后，一个reviewer说很好没有任何意见，另一个说没有新意，结果editor以”没有新意“为由给毫不留情的据了。
和老板商量了准备换个杂志投，老板还是觉得novelty是个问题，不反对试别的好杂志但他个人建议赶快投个容易接受的杂志。可是俺实在不忍心看着这么多工作好几年的心血就去投个小
杂志。所以还想试试好一点的杂志，找了找，觉得cell host and microbe 和 PloS
Biology从IF上看都还很不错的。不过不知道这两个杂志是不是都很难投啊？有知道的
大侠给说说吧？例如是不是对novelty要求很高？
另外，如果immunity据了以后，投同是cell press的cell host and microbe 杂志，
editor是不是会知道原来投immunity被据的事？那是不是会很吃亏啊？
谢... 阅读全帖 |
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