n********u
发帖数: 5 | 1 I need to pass a cell matrix with variable size (m X n) to a own defined VBA
fuction myfun(dataRange as Range). In this function the selected cell
matrix will be stored in an data array. I used to use Range.CurrentArray or
Range.Value2 to store the data into an assigned array variable successfully.
However, it requires the cells contain no formula. If the data in the cells
are obtained by a formula, everything screws up.
The code is shown below:
‘The function stores the data in selected cell mat |
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H*****e
发帖数: 120 | 3 Finally!
Amy is a star for long time. But almost like a joke although she and her
supporters fighted hard. Many years ago, she wrote a Cell review saying no
transdifferentiation. A few weeks later, there were 2 science paper did
transdifferentiation. When Cell launched Stem Cell, she was in the editor
board then she was elected for the senior post position in stem cell
association. How many AP could get this kind of treatment? I guess she
must make a big mistake in it this time.
Another ri... 阅读全帖 |
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a****d
发帖数: 1919 | 4 I don't think cancers are monoclonal, most times they are heterogeneous
population, and cancer can be derived by different cell lineages, at least
in some types of cancer.
Plus I don't think people in cancer stem cell field thought the "cancer stem
cell" have the ability to differentiate into multiple cell types. They are
more like cancer initiating cells per se.
differentiate |
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h********n
发帖数: 4079 | 5 BTW, I think the key is how to validate the cells isolated by whatever
methods. My thought is in vivo and orthotopic implantation.
1.Whether stem cell is cancer stem cell is an issue. I read so many
literature that people are using tissue stem cell marker for identify cancer
stem cells. Frankly, I reject all of them, manuscript or grant application
. |
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P*****l
发帖数: 438 | 6 http://www.nytimes.com/2010/10/15/science/15retract.html?_r=1
3 Harvard Researchers Retract a Claim on the Aging of Stem Cells
By NICHOLAS WADE
Published: October 14, 2010
Harvard researchers have retracted a far-reaching claim they made in January
that the aging of stem cells might be reversible.
The retraction was published in Thursday’s issue of Nature and is signed by
the senior author, Amy J. Wagers, and two others. They say that serious
concerns, which they did not specify, have undermined... 阅读全帖 |
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t******y
发帖数: 716 | 7 ips 基本上从原代foreskin cell里来，而原来foreskin cell很有可能就是skin stem
cell。所以，ips的过程只是扩展了skin stem cell的分化潜能，而不是改变其分裂性
能。
a
cell
normal |
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a*****g
发帖数: 543 | 8 It is interesting you mentioned the genetically similarity within cell line
s. Most of these studies would have been done using millions (if not billio
ns )of cells. The bias of sequencing or SNP would lead to identify the most
obvious low hanging fruit, such as KRAS, pten, p53 etc.
Apparently when single-cell level sequencing becomes widely available, iden
tifying subpopulations with genetic or epigenetic characteristics will be i
n a much better position.
http://www.genomeweb.com/sequencing/si... 阅读全帖 |
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h********n
发帖数: 4079 | 9 if AT2 cell is stem cell, there will be too many stem cells.
AT2 cell should be differentiated cell.
all |
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h*****G
发帖数: 113 | 10 Thank you.
Lineage reprogramming is definitely very promising. As mentioned in another
post, it has been showed that fibroblast can be transformed to neuron
with only 3 TFs.
At the same time, direct programming from ES/iPS cells to somatic cells are
few. And I was not be able to find any publications. So was wondering if
someone can help me with some papers about direct programming from ES/iPS
cells to somatic cells with TFs, not stepwise extracellular signals.
Thanks.
转换 |
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s*****g
发帖数: 7857 | 11 On your histogram:
PI+only (%#) --necrosis (dots on the Left side top)(died)
PI+/Annexin-V+ (%#)--later apoptosis (dots on the Right side top)(died)
PI-/Annexin-V+(%#) --early apoptosis (dots on the Right side bottom)(Not died)
PI-/Annexin-V- (%#)--alive & No apoptosis cells (health cells)(dots on the Left side bottom)
I use The sum of "PI+/Annexin-V+ (later apoptosis)" and "PI-/Annexin-V+(
early apoptosis)" to count Total Apoptosis (%)
You do not need do permeablize. If you permeablize, all ce... 阅读全帖 |
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f**g
发帖数: 28 | 12 Hi everyone,
I made some stably transfected cell lines with 293 cells. One cell line is
difficult to freeze down. Most cells were dead after frozen with 10% DMSO
in serum. Other cell lines are pretty normal. So anyone can give
suggestion on this problem?
Many thanks in advance. |
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a****d
发帖数: 1919 | 13 depends on the size of cell aggregates. dual cells or triple cells are fine
for cell sorting. But big cell aggregates can't pass through and may get
machine jammed. |
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s****m
发帖数: 472 | 14 Nature Cell Biology!
Cell Stem Cell is 暴发户 and 跟风灌水 |
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f*****Y
发帖数: 20 | 15 胚胎是一个比cell culture复杂的多的系统，需要很多signaling的协调才能正常的发
育。而ES cells只是胚胎的一个cell lineage的culture adaptation，是一个简单的多
的系统。比如说，cdx2 mutant embryo因为trophectoderm defect 无法存活(
embryonic lethal E3.5-5.5), 但是cdx2 ES cells 是可能的。还有nanog mutant
embryo is lethal，Nanog null ES cells 据说可以proliferate many passages. |
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A******d
发帖数: 571 | 16 I am working with a mouse liver cell line (Hepa1-6) from ATCC. There are
always clumps of cells in the flask here and there. I tried pipeting up and
down- didn't work. I even vortexed the cell suspension- cells are still
clumped.Anyone has experience of breaking up clumped cells? Thanks! |
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h********n
发帖数: 4079 | 17 1) I don't know. There is no solid data about cell phone radiation's
physiologic affect.
2) I don't know either.
I guess the simple experiment is to give cultured cell line radiation (mimic
those of cell phone) for months or longer and see any effect. I guess some
people did it but did not find anything interesting.
epi is not the only thing we can look at, but currently epi data is the data
we can have. No mechanistic research. On the other hand, for smoking,
there are solid evidence: if y... 阅读全帖 |
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A******y
发帖数: 2041 | 18 Resazurin, MTT, MTS etc. are all based on mitochondria respiration. If your
cell is attached, I will just do PI stain. You can also count the cell
directly with trypan blue etc.
However, I can tell you the reviewer is been difficult. I bet you already
count the cells and your compounds do inhibits cell growth....I haven't seen
a single compound that could lower the mitochondria function so much that
will affect the MTS assay if it is not directly reflective toward cell
numbers. |
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s**u
发帖数: 9035 | 19 The New York Stem Cell Foundation Requests Applications for: Innovator
Awards for Early Career Investigators
WEBSITE: http://iem.ucsd.edu/system/assets/53/NYSCFStem%20Cell%20Investigator%20RFA_2011.pdf
The New York Stem Cell Foundation Requests Applications for: Innovator
Awards for Early Career Investigators
NYSCF is soliciting applications from early career investigators for
awards that will cultivate research exploring the therapeutic
potential of human stem cells. The main goal of this initi... 阅读全帖 |
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c********r
发帖数: 1125 | 20
你说的没错，领域不同。很多专业杂志换了领域就每人知道
但是我强调得是“几乎没人听说过”，我们实验室将近20个人，做得是细胞／遗传／生
化。。。18个人没听说。。。这也算侧面证明cell research影响力不行吧？毕竟领域
接近啊。
而且不能完全用“新杂志”来解释，很多cell和nature子刊得新杂志因子也不是那么高
，但是在领域里影响还不错。
大家就像一个身边得例子吧，你的研究文章的重要文章那篇综述或者research文章是引
用cell research上得？
当然我不是说cell research就不好，我知道很多国内得实验室被cns screw 得文章就
发到cell research上面 |
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l**********1
发帖数: 5204 | 21 然后combine to one single PDF file?
BTW,
LZ 用斜体或异色等方式直接引用一一reviewer的问题和原话
然后一一用正体 黑色码字回复即可
范本如下:
To:
Dr. XXX Editor
nnn mmm Journal
Changes made in revised manuscript
Bla bla bla
Changes made in response to the comments by Reviewer 1:
Bla bla la
Changes made in response to the comments by Reviewer 2:
Bla bla la la
Changes made in response to the comments by Reviewer 3:
Bla bla la la la
Additional changes:
nnn mmm
Changes made to Supporting Material:
xxx zzz
----
发信人: spt009 (cytokine), 信区: Biolog... 阅读全帖 |
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l*******i
发帖数: 153 | 23 首先有一个概念要明确：目前说的ES细胞，都是in vitro culture的，与in vivo的不
一样。
ES细胞分为naive和prime两个stage
1)naive stage：ES colony在形态上看起来更“致密”，呈三维结构，对胰酶不敏感（
即胰酶消化不会诱导其凋亡）。对小鼠ES而言，naive的ES来源于inner cell mass(ICM
)。此stage的ES self-renewal更迅速，population double大概只需要12-14小时。对
于其self-renewal的维持需要LIF，stat3,BMP4等。雌性小鼠naive ES的另一个显著特
点是X chromosome reactivation,而在其他类型的细胞中有一条X chromosome是失活的
。从全能性角度来讲，naive ES是真正具有全能性的，能形成完整的个体。
2）prime stage：对小鼠而言，其来源于postimplantation epiblast,是naive stage
的下一个阶段，其形态看起来与naive ES明显不一样，呈“二维”结构，即colony... 阅读全帖 |
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j*b
发帖数: 341 | 24 这个PKM2和肿瘤代谢的关系已经知道很久了，这两篇还不是原创贴。
cantley还可以发nature，能不能解释一下？
Anticancer Res. 2003 Mar-Apr;23(2A):1155-8.
Tumor marker pyruvate kinase type tumor M2 in patients suffering from
diabetic nephropathy.
Oremek GM, Rutner F, Sapoutzis N, Sauer-Eppel H.
Source
Central Laboratory, Hospital of the Johann Wolfgang Goethe-University,
Frankfurt/Main, Germany.
Abstract
INTRODUCTION:
Compared to normal tissues all tumors investigated so far and especially
those with a metastatic potential overexpress an isoenzy... 阅读全帖 |
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j*b
发帖数: 341 | 25 这个PKM2和肿瘤代谢的关系已经知道很久了，这两篇还不是原创贴。
cantley还可以发nature，能不能解释一下？
Anticancer Res. 2003 Mar-Apr;23(2A):1155-8.
Tumor marker pyruvate kinase type tumor M2 in patients suffering from
diabetic nephropathy.
Oremek GM, Rutner F, Sapoutzis N, Sauer-Eppel H.
Source
Central Laboratory, Hospital of the Johann Wolfgang Goethe-University,
Frankfurt/Main, Germany.
Abstract
INTRODUCTION:
Compared to normal tissues all tumors investigated so far and especially
those with a metastatic potential overexpress an isoenzy... 阅读全帖 |
|
z****u
发帖数: 1007 | 26 我觉得在identify Pax7 + satellite cell 之后，之前的很多myoblast cell culture
protocol可能需要re-consider了。比如上面那个protocol,neonatal mice limb
muscle.分离出来的很有可能是混杂了satellite cell以及committed myoblast cell的
mixture，而到底是哪种细胞长起来就很难说了。
adult的myoblast cell culture好像没有吧。 我没有仔细查过文献，只是大概的尝试
过几次，觉得从adult muscle fiber分离出来的细胞体外很难长。 |
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l********7
发帖数: 175 | 27 Relapesed cancer cells do not express cd19 in one patient. Cd19 car-t cell
works better than other antigen Car-t cells, especially some solid tumor
targeting Car-t cells. Now it is a ho topic link in cell based therapy. |
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d*****s
发帖数: 647 | 28 我们学校做中期考核，全校所有高层次人才（物理+化学+工科+生科+医科等）都要汇报
工作，说到cell，非生物专业的人才很多不熟悉这个杂志，他们充其量认为cell是生物
学领域的顶级期刊，而nature和Sceince是全领域的顶尖期刊，比cell高一档。
后来仔细想想，他们非生物人士说的也对，毕竟nature和Science是多领域杂志，cell
也就和Nature有关生物医学的子刊（20以上的）一个档次吧！
把cell抬到nature和Science等同的地步，有点降低NS的档次！ |
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c**********0
发帖数: 7 | 29 cell biology, stem cells, iPS cells, regenerative medicine, developmental
biology, tissue engineering, cardiovascular regeneration, pulmonary
hypertension, cardiac cells and endothelial cell differentiation等相关领域都
可以, 长期有效
请联系c**********[email protected]
十分感谢!!! |
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i*****i
发帖数: 154 | 30 commercial的competent cell太贵了，想自己做一些。
把买来的competent cell 摇起来一些，然后做competent cell，但是效率似乎非常的
低，不知道版上有没有人有过类似的经验。我之前一直自己做competent cell （不同
菌株），效率非常非常的高。
是不是要传代几次啊？ |
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t****w
发帖数: 130 | 31 Transient amplifying cell是普遍存在的，比如Hans研究得很清楚的small intestine
，+4位置以上的一般被认为是TA细胞。
如果multipotent的标准都不要了，hierarchy的标准也不要了，那已经没有探讨stem
cell的意义了。就像在liver和pancreas里边一样，每个cell都可以是stem cell， all
cells are born equal！ ———— 这显然歪曲了学科的本质。
hierachical
lineage
mouse |
|
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s**********e
发帖数: 140 | 33 1.stem cell会衰老吗？
2.stem cell会随着所属生命体年龄的增大而衰老吗？
培养皿中的stem cell分裂为两个daughter cells,
其中一个daughter cell会比另一个更衰老吗？(大肠杆菌就有这种现象) |
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t******k
发帖数: 599 | 34 We, the editors of Cell, published an Editorial Expression of Concern http://dx.doi.org/10.1016/j.cell.2016.03.038 earlier this year regarding issues raised about Figures 2F, 2H, and 3G of the above article. Dr. Yao-Yun Liang, who performed the experiments in question, claimed to have manipulated his experiments to achieve pre-determined results. The corresponding author, Dr. Xin-Hua Feng, refuted the claims of falsification. In the intervening time, two independent labs have carried out exper... 阅读全帖 |
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发帖数: 1 | 35 最近genotype一个cancer cell line（neuroblastoma）的microsatellite （tandem
repeat）位点
大概三个月前测的是这个位点是homozygous有6个repeat
然后最近重新解冻另外一个stock，start cell culture，测量同样的位点，居然成了
heterozygous，一半仍然6个repeat，另外一半成了10个repeat；反复做了很多遍，换
了所有的reagent生怕是contamination，但结果还是一样。
问了lab technitian，这个neuroblastoma在我们实验室都是一样的来源，他也怀疑这
个repeat是不是dynamics的
三个月前的cell line的passage不记得了
最近这个cell line的passage大概是p10
我想问下，cancer cell line的genome microsatellite难道真的会传代传着传着就发
生了变化？如此不稳定？ |
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t****m
发帖数: 88 | 36 谢谢楼上各位的建议！如果cell systems的影响因子可能也不会比cell metabolism高
，那就还从高的开始投吧。我本来以为cell systems能上20呢…… |
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M*******C
发帖数: 183 | 37 我想做T cell 和 cancer cell co-culture, 本来想用RPMI 1640 medium（因为看到文
献好像T细胞都是用1640），但lab过去的cancer cell 都是用DMEM （corning 10-013-
CV），如果我现在用1640，就会导致实验condition改变了，跟原来的cancer cell的实
验不好比较。
但我没看到用DMEM来养T细胞的，想问问我能不能用DMEM （加上IL-2之类T cell需要的
因子。。）来做我的co culture？如果不能，为什么？谢谢！ |
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m***r
发帖数: 225 | 38 The major issue is the cost. They are other alternatives, particularly
polymer based solar cells. However, their efficiency is low (~6% in the
lab, ~3% in modules).
Si solar cell is just too costly. However, their life time (20-30 yrs)
is very good. So I think Si solar cell is still the top choice for
"solar" buildings. If polymer solar cells want to compete, you can try
to make "solar" window, which can be replaced every 2-3 years (given the
cost of polymer solar cell can be significantly |
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r*****s
发帖数: 262 | 39 可以这样理解吗？
就拿smic18为例。
1.8v标准数字管 （就一种）
3.3v i/o管 （就一种）
Std Digital Cell Lib 是由1.8v标准数字管组成的
smic18 mixed signal工艺由
3.3v i/o管 + 1.8v标准数字 Std Digital Cell Lib
组成的
smic18 EEPROM工艺
3.3v i/o管 + 1.8v标准数字 Std Digital Cell Lib + EEPROM这个特殊工艺
组成的
smic18 BCD工艺
3.3v i/o管 + 1.8v标准数字 Std Digital Cell Lib + Bipolar CMOS这个特殊工艺
组成的
smic18 高压工艺
3.3v i/o管 + 1.8v标准数字 Std Digital Cell Lib + 高压管这个特殊工艺
组成的
对吗？
Lib |
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s**********e
发帖数: 140 | 40 1.stem cell会衰老吗？
2.stem cell会随着所属生命体年龄的增大而衰老吗？
培养皿中的stem cell分裂为两个daughter cells,
其中一个daughter cell会比另一个更衰老吗？(大肠杆菌就有这种现象) |
|
P*****s
发帖数: 375 | 41 Capturing the Promise of Youth
Gretchen Vogel
In 1999, researchers recognized the extraordinary potential of stem cells,
immature cells with the ability to become different kinds of tissue--and
perhaps to heal many kinds of illness
Old age may have wisdom, but it will always envy youth for its potential. The
same might be said for cells, for a young cell's most important trait is its
ability to choose among many possible fates and become, say, a neuron passing
electrical signals or a blood cell |
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s**********e
发帖数: 140 | 42 1.stem cell会衰老吗？
2.stem cell会随着所属生命体年龄的增大而衰老吗？
培养皿中的stem cell分裂为两个daughter cells,
其中一个daughter cell会比另一个更衰老吗？(大肠杆菌就有这种现象) |
|
s******h
发帖数: 23 | 43 http://www.bbc.com/news/health-26516458
A Japanese scientist behind a seemingly groundbreaking stem cell study says
the findings should be withdrawn amid doubts over its quality.
It was reported in January that dipping cells in acid could cheaply and
quickly convert them into stem cells.
But questions were raised about the images used in the scientific report and
other research groups have failed to reproduce the results.
Author Prof Teruhiko Wakayama said: "It is no longer clear what is right."... 阅读全帖 |
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f***y
发帖数: 4447 | 44 那位千老那么恶毒？
http://news.bioon.com/article/6689623.html
9号凌晨，国际顶级期刊《Cell》在最新一期杂志中发了一则“Editorial Note”，报
道称有两个实验室独立重复出了冯新华实验室2006年Cell文章中的作者之一梁耀云声称
通过操纵实验得到的图2F，2H和3G中的结果。这则声明的原文描述是“Those two labs
have now completed their experiments， and their data largely confirm the
central conclusions drawn from the original figures”，Cell表示基于这些重复
的实验将不再有进一步的行动（take no further action），并对这两个独立进行实验
重复的lab表示了感谢。 |
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l****z
发帖数: 29846 | 45 PepsiCo has come under fire from pro-life advocates because it has been
contracting with a research firm that uses fetal cells from babies
victimized by abortions to test and produce artificial flavor enhancers.
Now, the Obama administration is set to face more criticism because an
agency has declared that Pepsi’s use of the company and its controversial
flavor testing process constitutes “ordinary business.”
In a decision delivered February 28, the Security and Exchange Commission
ruled that Pe... 阅读全帖 |
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t*********u
发帖数: 26311 | 46 星期一，苹果宣布iPhone 5周末首发销量为500万。虽然这个数据远优于去年iPhone 4S
的首发创下的成绩，但是它却没有达到分析师预计的目标。苹果股票也因此出现了波动
，而分析师则将“销量不佳”的原因归结于iPhone 5货源供应紧张。而iPhone 5供不应
求自然也有其原因。
日前巴克莱银行分析师Ben A. Reitzes在向投资者发布报告中指出，iPhone 5供应
紧张是因为in-cell屏幕供应的限制。在报告中该分析师表示目前9月季约有1000万屏幕
，而在12月季屏幕供应量会大幅增加。
Reitzes认为第四季度in-cell屏幕供应量增加之后，苹果iPhone销量将会增加至45
00万部，与去年同比增长22%。此前的消息显示iPhone5的in-cell触摸屏由LG Display
Co.和Japan Display Inc.生产供应的，彭博社曾经向上述两家公司以及苹果公司证实，
但是他们都不予回复。
iPhone 5所使用的in-cell触摸技术有利于减小机身厚度，这也是为什么这次我们
看到iPhone 5比上一代更轻更薄的原因。 |
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b**********5
发帖数: 7881 | 48 A Blob is a shape in two-dimensional integer coordinate space where all
cells have at least one adjoining cell to the right, left, top, or bottom
that is also occupied. Given a 10x10 array of boolean values that represents
a Blob uniformly selected at random from the set of all possible Blobs that
could occupy that array, write a program that will determine the Blob
boundaries.
Optimize first for finding the correct result, second for performing a
minimum number of cell
Boolean value reads, and ... 阅读全帖 |
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b**********e
发帖数: 108 | 49 A postdoctoral position in Microfluidics for Single-Cell Analysis
The project is focused on developing new microfluidic platforms and
molecular bioassays for quantitative single-cell analysis of cancer. Their
applications to cancer pathology and clinical diagnostics will be pursued in
collaboration with KU Medical Center. A recent Ph.D. in chemistry,
biochemistry, molecular/cell biology, bioengineering or biomedical sciences
is required. The applicants should have strong interest and expertise i... 阅读全帖 |
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发帖数: 1 | 50 Cell Research is seeking two full-time, in-house scientific editors to join
its editorial team. The Scientific Editor will be trained to play major
roles in providing immediate and satisfactory supports to the Editor-in-
Chief and Deputy Editor-in-Chief in all aspects of manuscript processing,
which include but are not limited to assessing submitted papers, overseeing
the reviewing process, and commissioning research highlights and review
articles. The key qualities the journal looks for are bre... 阅读全帖 |
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