w********r
发帖数: 1431 | 1 最近没事，看了几片beta cell的paper，
发现关于beta cell stem/progenitor cell的争论还挺热闹的。
顶尖杂志上经常你来我往灌水争论这个progenitor cell到底存在不存在。
04年Melton DA组的一片nature用了Insulin的reporter mice做了genetic tracing，说
新生的beta cell都是从existing beta cell长出来的，
但08年Xiaobo Xu的Cell比较清楚的证明了progenitor cell的存在，
11年3月的Simon R. Smukler的cell stem cell和说这个细胞是存在的，而且直接说
Melton当年nature paper里面的老鼠有问题。不过看了11年CSC的文章从submit到接受
用了差不多两年，不知道是不是业内反对派的能量太大啊？
CSC配了一片previews，看上去也是各种抹稀泥，各打五十大板，
说progenitor cell是存在的，但是differentiated beta cell自己duplicate也是存在
的。
我一外行... 阅读全帖 |
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c*****t
发帖数: 1084 | 2 generally speaking progenitor cells are dervied from stem cells. the fate of
progenitor cells are more determined than stem cells and as a consequence they
can only differetiated into a few cell types. while stem cells have much
more potential to differentiate into various cells and organs. it's all about
potential. |
|
j******g
发帖数: 171 | 3 No.
Now, it's suggested to use somatic stem cells concept than adult stem cells.
As far as I know, progenitor cells are of less differentiation
potential(multipotency, just many progenies but not all), while stem cells are
of pluripotency (All of the progenies). So in this matter, PC is considered
more committed, determined, or differentiated than SC. However, most time ppl
find adult stem cells which are really progenitor cells.
One also needs to clarify precursor cells (or simply precursors), |
|
h******y
发帖数: 1374 | 4 Scientists turn human skin into blood
Scientists at McMaster University have discovered how to make human blood
from adult human skin.
The discovery could mean that people needing blood for surgery, cancer
treatment or treatment of blood conditions like anemia will be able to have
blood created from a patch of their own skin to provide transfusions.
Clinical trials could begin as soon as 2012.
The researchers have also shown that the conversion is direct. Making blood
from skin does not require ... 阅读全帖 |
|
w********r
发帖数: 1431 | 5 in the case of beta cell, I think it is more than "literally" argument ...
Existing beta cell dublication and progenitor cell differentiation are
totally two concepts. For the name of muscle satellite cell/primary myoblast
/muscle stem cell, we are also confused. So we call them "muscle progenitor
cell" in our recent paper... lol
exist
work
correct one here though), on muscle satellite/stem cells, about 6 ys ago,
one prominent stem cell biologist(I am sure that many on this board knows
him) we... 阅读全帖 |
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d*******e
发帖数: 85 | 6 一个2分多的生物学方面的杂志，与FGF2 和endothelial progenitor cells有关。
感兴趣的xdjm可以发一份简要CV和联系方式给我，我会优先推荐。 |
|
j******g
发帖数: 171 | 7 Pluripotency and Totipoteny has similar meaning if not the same, i.e.
differentiation potential of all lineages but not SC of all different cell
types. But some ppl prefer to use Totipotency for ESC.
As I mentioned before, some of the adult stem cells are NOT true stem cells,
coz they only have limited self-renewal ability and differentiation potential.
So it's probably more approtiate to call them progenitor cells. Most time the
Sci would like to blow they find xxx type of stem cells, however, |
|
j*********o
发帖数: 237 | 8 想做一个实验，需要把小鼠ES细胞诱导成neural progenitor和neuron，只知道一般使
用RA诱导，但找不到具体的protocol，版上有没有哪位做过有比较完整的protocol？能
不能分享一下，非常感谢！如有请发到d********[email protected]。多谢多谢！！ |
|
a****d
发帖数: 1919 | 9 小文章一篇，cortex development, progenitor cell相关，求杂志推荐，能发表即可
，目前考虑plos one, neuroscience, cerebral cortex这几个。熟悉的请推荐一下，
审稿速度快不拖沓最好，谢了！ |
|
|
z*t
发帖数: 863 | 11 HSC体外培养就分化了...冻起来是别想了...不过如楼上所说大概在一周之内还会有一
定数目的progenitor的，transduce之后sort cell不知道能拿多少细胞，我们lab有人
做过 1X105 HSC的western |
|
A******d
发帖数: 571 | 12 iPS cells, glycocorticoids, and/or neuronal progenitor cells
有没有以上关键字方面的专家？感兴趣的同学请把相关简历粘帖发给我站内信箱，我会
发出审稿邀请。谢谢！ |
|
w*****3
发帖数: 1582 | 13 李铁（ironman)献了25次了，我现在争取每年冬歇的时候献。献血的好处还是很多的，
锻炼造血机能，更新旧的免疫细胞，稀释血液。不好的地方是刚献完血，会有抵抗力轻
度低下， 运动能力也会降低。
献血以后，液体电解质当时就补足了，血浆蛋白需要1周，细胞需要1个月。
献血一般认为会降低心血管病的危险，这篇文章认为献血也会降低肿瘤的发生。
老的T细胞如果不被新生取代的话，容易生成tumor。
Cell competition is a tumour suppressor mechanism in the thymus
Cell competition is an emerging principle underlying selection for cellular
fitness during development and disease. Competition may be relevant for
cancer, but an experimental link between defects in competition and
tumorigenesis is elusive... 阅读全帖 |
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f******g
发帖数: 1003 | 14 老的T细胞如果不被新生取代的话，容易生成tumor。
Cell competition is a tumour suppressor mechanism in the thymus
Cell competition is an emerging principle underlying selection for cellular
fitness during development and disease. Competition may be relevant for
cancer, but an experimental link between defects in competition and
tumorigenesis is elusive. In the thymus, T lymphocytes develop from
precursors that are constantly replaced by bone-marrow-derived progenitors.
Here we show that in mice this turnover is regula... 阅读全帖 |
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a****d
发帖数: 1919 | 15 Those are very general questions.
For stem cells/progenitors, self-renewal and proliferation are two distinct
events, although many people don't separate these two when talking about
behavior of stem cells/progenitors. If you ask about proliferation of stem
cells, it seems pretty much similar pathways also regulate tumorgenesis. To
be more specific, differentiation of stem cells/progenitors also mimics EMT,
at certain level. Angiogenesis is ongoing along with progenitors
proliferation, same as t... 阅读全帖 |
|
b*******1
发帖数: 16 | 16 岗位一：吴虹课题组博士后工作人员 （2名）
主要职责：
1）独立开展科研工作，动手进行体内外实验；
2）协助PI负责实验室研究课题的设计和开展；
3）参与科研基金撰写；
4）协助培养研究生。
招聘条件：
1）品学兼优，身体健康，年龄在40岁以下；
2）近年来在国内外获得分子生物学、细胞生物学、肿瘤遗传学、药理学等相关专业博
士学位；
3）至少具备一项以下领域内的工作经验和文献发表：
a. 独立运用细胞和动物模型进行肿瘤药物的机理研究
b. 在分子生物学和细胞生物学领域有深厚的知识背景；
c. 在蛋白质分析、核酸分析和细胞生长分析等相关技术方面有丰富的经验；
d. 对治疗癌症的药物疗效和安全性评价方面有强烈的兴趣和研究背景 ；
4）有较强的团队合作精神，沟通协调能力强，动手能力强，工作细心，积极主动，能
持之以恒；
聘用待遇：
本职位将为你提供在生物学领域极具竞争力和创造性的工作机会。博士后工作人员薪酬
标准按照北京大学生命科学院和北大-清华生命科学中心相应规定及个人工作经验及能
力确定。
联系方式：
有意者请将本人应聘材料电子文档发至以下联络方式（请在应聘材料及邮件主题上注明
：姓名 ... 阅读全帖 |
|
b*******0
发帖数: 125 | 17 NATURE | ARTICLE
Hypothalamic stem cells control ageing speed partly through exosomal miRNAs
Yalin Zhang, Min Soo Kim, Baosen Jia, Jingqi Yan, Juan Pablo
Zuniga-Hertz, Cheng Han & Dongsheng Cai
Abstract
It has been proposed that the hypothalamus helps to control ageing, but the
mechanisms responsible remain unclear. Here we develop several mouse models
in which hypothalamic stem/progenitor cells that co-express Sox2 and Bmi1
are ablated, as we observed that ageing in mice start... 阅读全帖 |
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f**********g
发帖数: 2252 | 18 anyone familar?
Progenitor Cell Therapy to Present at 6th Annual New York Stem Cell Summit
on Tuesday, March 1
1 days 6 hours 27 minutes ago - EUR via Comtex
PR Newswire EuropeNeoStem, Inc. , an international biopharmaceutical company
with operations in the U.S. and China, announced today that Dr. Robert
Preti, PhD, President and Chief Scientific Officer of Progenitor Cell
Therapy ("PCT"), a wholly-owned subsidiary of NeoStem, Inc., has been asked
to present on Tuesday, March 1, 2011 at the 6th ... 阅读全帖 |
|
f**********g
发帖数: 2252 | 19 anyone familar?
Progenitor Cell Therapy to Present at 6th Annual New York Stem Cell Summit
on Tuesday, March 1
1 days 6 hours 27 minutes ago - EUR via Comtex
PR Newswire EuropeNeoStem, Inc. , an international biopharmaceutical company
with operations in the U.S. and China, announced today that Dr. Robert
Preti, PhD, President and Chief Scientific Officer of Progenitor Cell
Therapy ("PCT"), a wholly-owned subsidiary of NeoStem, Inc., has been asked
to present on Tuesday, March 1, 2011 at the 6th ... 阅读全帖 |
|
x*****a
发帖数: 972 | 20 测了一种细胞的一个surface marker，重复了很多次，都是negative，positive
control没问
题。应该就是negative的了。该marker是mature cell的，而我的细胞理论上来说应该
是该种细胞
的stem/progenitor cell。
literature research发现，从来没人报道stem/progenitor表达（或者不表达）这个
marker；
有的人induce differentiation后测，是positive的，以此反证其原来的population中
有该
stem/progenitor，而不直接测，也不解释为什么这么做。
我个人的看法，应该不太可能没人测过，可能就是negative的，但是大家都不发表这个
结果。
我打算不发表这个结果，主要是避免reviewer在这个问题上纠结。如果发表这个，我只
能claim说这
个细胞就是Negative（这算是我个人的学术观点），但是我目前又没有hard evidence。
赶毕业，没时间做更多的实验，目前的实验技术，也很难证明我的这个观点。
这个做法，有问题么？ |
|
r*****i
发帖数: 117 | 21 thank you for your suggestions.
Differentiation of myeloid progenitor into monocyte/osteoblast/osteoclast
could be influenced. We have litter evidence for it, but not enough.
We found there is more osteoclast in KO mice compared to WT ctl at the same
age. and bone density is less compared to control mice.
As for your suggestion: Meyloid progenitor 分化成monocyte/osteoblast/
osteoclast那一类细胞的分化通路受到影响了么.
How can we check it?
Here is my plan: check MPP, CMP, MEP, GMP and monocyte number for KO and
cont... 阅读全帖 |
|
e****i
发帖数: 2152 | 22 【 以下文字转载自 Military 讨论区 】
发信人: erkuai (三天不上bbs,赶得上刘少奇), 信区: Military
标 题: On the Jews and Their Lies, 1543
发信站: BBS 未名空间站 (Wed Jan 12 23:21:23 2011, 美东)
by Martin Luther (1483-1546)
Translated by Martin H. Bertram
copyright © 1971 Fortress Press & Augsburg Fortress - On the Jews and
Their Lies is from Luther’s Works Volume 47. Augsburg Fortress is the
publishing ministry of the Evangelical Lutheran Church in America.
Funded through sales revenue, Augsburg Fortress is called to provide
produ... 阅读全帖 |
|
e****i
发帖数: 2152 | 23 by Martin Luther (1483-1546)
Translated by Martin H. Bertram
copyright © 1971 Fortress Press & Augsburg Fortress - On the Jews and
Their Lies is from Luther’s Works Volume 47. Augsburg Fortress is the
publishing ministry of the Evangelical Lutheran Church in America.
Funded through sales revenue, Augsburg Fortress is called to provide
products and services that communicate the Gospel, enhance faith, and
enrich the life of the Christian community from a Lutheran perspective.
Part I
I had mad... 阅读全帖 |
|
C*I
发帖数: 4736 | 24 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 25 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 26 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 27 Published: 30 October 2013
Isolation and characterization of a bat SARS-like coronavirus that uses the
ACE2 receptor
Xing-Yi Ge, Jia-Lu Li, Xing-Lou Yang, Aleksei A. Chmura, Guangjian Zhu,
Jonathan H. Epstein, Jonna K. Mazet, Ben Hu, Wei Zhang, Cheng Peng, Yu-Ji
Zhang, Chu-Ming Luo, Bing Tan, Ning Wang, Yan Zhu, Gary Crameri, Shu-Yi
Zhang, Lin-Fa Wang, Peter Daszak & Zheng-Li Shi
Nature volume 503, pages535–538(2013)Cite this article
Abstract
The 2002–3 pandemic caused by severe acute respirator... 阅读全帖 |
|
C*I
发帖数: 4736 | 28 哪里？ 看好了，在这里！ 他们完整的分离并拥有了这个病毒！
Nature
Published: 30 October 2013
Isolation and characterization of a bat SARS-like coronavirus that uses the
ACE2 receptor
Xing-Yi Ge, Jia-Lu Li, Xing-Lou Yang, Aleksei A. Chmura, Guangjian Zhu,
Jonathan H. Epstein, Jonna K. Mazet, Ben Hu, Wei Zhang, Cheng Peng, Yu-Ji
Zhang, Chu-Ming Luo, Bing Tan, Ning Wang, Yan Zhu, Gary Crameri, Shu-Yi
Zhang, Lin-Fa Wang, Peter Daszak & Zheng-Li Shi
Nature volume 503, pages535–538(2013)Cite this article
Abstract
The 2002–3 pande... 阅读全帖 |
|
C*I
发帖数: 4736 | 29 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“
WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他... 阅读全帖 |
|
v*******n
发帖数: 8995 | 30 好像非常合适我的背景，请考虑一下我， 谢谢
ying wang
[email protected]
/* */
背景是molecular biology，cancer(ovarian cancer, tumor metastasis)/stem cell
biology
文章有molecular cell 和cancer research
1. Wang Y, Arribas-Layton M, Chen Y, Lykke-Andersen J, and Sen GL.DDX6
Orchestrates Mammalian Progenitor Functionthrough the mRNA Degradation and
Translation Pathways. Mol Cell. doi:10.1016/j.molcel.2015.08.014
2. Mistry DS, Chen Y, Wang Y, Sen GL. Transcriptional profiling of SNAI2
regulated genes in primary human kerat... 阅读全帖 |
|
s***b
发帖数: 2 | 31 对于海马区来说,有没有真正的multipotent NSCs 还存在争论,即使是Gage现在也改称
之为progenitor了.
而block掉adult neurogenesis已经有方法了,而且以后会更多,见这篇文章(Garcia et
al., 2004)
Garcia, A.D., Doan, N.B., Imura, T., Bush, T.G., and Sofroniew, M.V. (2004).
GFAP-expressing progenitors are the principal source of constitutive
neurogenesis in adult mouse forebrain. Nat Neurosci 7, 1233-1241.
目前NSCs 在成体大脑里真正的研究热点是证明脑瘤来源于成体神经干细胞, 已经大部
分脑瘤的位置是十分接近SVZ的,(Zhu et al., 2005) Zhu, Y., Guignard, F., Zhao,
D., Liu, L., Burns, D.K., Mason, R.P., Messing, A. |
|
h********n
发帖数: 4079 | 32 I am so glad to read your thought. I would like to discuss a little more.
to 1: the term of CSC is controversy, but what if I say: "In lung adenocarcinoma, there is a population of tumor cells whose potency (in vivo) is much higher than other population in the same tumor. It is important to study this population of cells. How about we call it tumor initiating cell?"
what is your opinion to my above thought?
to 4: I don't agree with you on this point. Most lung cancer model use SPC or CCSP or C... 阅读全帖 |
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r***e
发帖数: 2539 | 33 Thank everyone for the discussion!
As pineseed pointed out, too many topics were discussed here.
But my personal interests are:
1. what is the cell origin of different type of lung cancers?
2. does cell origin or mutation decide tumor types?
3. how to model different type of lung cancer?
HGTPase, it seems you are working in this field (more lung development than
lung cancer?), and I hope you can give your opinions. We can also discuss in
private mails for technique details.
1. ... 阅读全帖 |
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h********n
发帖数: 4079 | 34 Why the concept of CSC is important in basic cancer research? To me, the
key question is: does a solid tumor (such as lung, liver, etc) arise from a
differentiated epithelial cell, or it arise from a tissue specific stem cell
/progenitor cell.
tissue specific stem cell/progenitor cell ---> differentiated epithelial
cell.
CSC--->differentiated cancer cells (with different potency).
Think of the comparison between these cells, especially the cancer vs normal
cells.
As to cancer cell lines, some o... 阅读全帖 |
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s******r
发帖数: 2876 | 35 不过自从iPS发明以后，我觉得solid tumor也可能起源于分化细胞。
Why the concept of CSC is important in basic cancer research? To me, the
key question is: does a solid tumor (such as lung, liver, etc) arise from a
differentiated epithelial cell, or it arise from a tissue specific stem cell
/progenitor cell.
tissue specific stem cell/progenitor cell ---> differentiated epithelial
cell.
CSC--->differentiated cancer cells (with different potency).
Think of the comparison between these cells, especially the cancer vs normal... 阅读全帖 |
|
f********s
发帖数: 115 | 36 你说的很多我都同意。只不过现在我想定量的的来分析一种特定的 progenitor （ex.
CMP） 分化到各个lineage 的能力。我觉得在把多个progenitor 接种到同一个盘子上
有几点非常影响我做定量分析：
1.你在挑集落的时候是可以看到集落的样子的，所以也许你会不自觉的多挑或者少挑某
些集落，引入bias;
2.在挑集落的时候，大的集落容易挑出来，小的集落不容易挑，即使挑了小集落在后续
实验过程中不知道怎么就不见了 (而且大小通常意味着不同的lineage)，也会严重影响
实验者的判断。
因此，我觉得一定要接种单细胞到96孔板里，在不看孔的情况下, 随机挑选比如说20个
孔来观察，这样可以得到各种百分比，比如说百分之多少的单细胞能形成集落，百分之
多少的细胞能形成红细胞集落，多少能形成粒细胞集落……不知道关于这一点你是否同
意？
在这个基础上，我想知道半固体和全液体培养基在这个实验里的优劣。我能想到的半固
体的坏处在于，1.如果你不看孔，不知道这个克隆在哪里，你就要把全部的200ul半固
体培养基吸出来，非常不利于cytospin. 2.而且半固体培养基会有一部分粘在t... 阅读全帖 |
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y****i
发帖数: 2194 | 37 你必须弄明白为什么你的ko老鼠比wt体重轻 （上Metabolic cage)
不然你所有关于糖代谢insulin的数据都没有意义
（因为你在比较两组体重不同的老鼠，瘦老鼠当然糖代谢比胖老鼠有改善）
ITT非常不敏感，而且像我前面说的，不管是positive还是negative结果都是没法解释
的。
至于fat progenitor，我觉得跟你的问题没有什么直接的联系。一个常见的误解是因为
“瘦”是脂肪发育defect造成的，其实绝大多数情况都是energy balance的问题。除非
很罕见的情况你有lipodsytrophy phenotype （如果你影响了fat progenitor的话），
但是这样的话老鼠应该又瘦又diabetic，你这个明显不是。
你的现象必须仔细分析food, activity和energy expenditure的变化，而且这个分析必
须在你的老鼠体重开始diverge之前做，一旦体重显著不一样了，后来的分析都没有什
么意义。 |
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x*****a
发帖数: 972 | 38 CD31在macrophage也有大量表达。如果看Murine macrophage，F4/80是个比较接受的
marker，但是F4/80在有些dendritic cell或者monocyte上也有表达，所以F4/80是个不
完全纯粹的marker。
CD34是个stem cell/progenitor cell的marker，如果是非常成熟的endothelial cells
，是不太表达这个的，你染出来的可能是较为后期的endothelial progenitor cell。
其实vWF就是最好的了，既然染出来了，就可以了啊。VE那个当然也行。 |
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f******g
发帖数: 1003 | 39 IDH1(R132H) mutation increases murine haematopoietic progenitors and alters
epigenetics.
Nature, 2012
These mutants show increased numbers of early haematopoietic progenitors and
develop splenomegaly and anaemia with extramedullary haematopoiesis.
表型不是特别明显。
是不是可以人为IDH1的突变只是helper mutation呢?
condition |
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s***l
发帖数: 10 | 40 刚刚在研究这个领域的文章，看到这个guang-hui liu在cell research上出现了几次
，频率很高，就搜了一下，结果吓了一跳，尽然有14篇之多。这个肯定是中国干细胞界
的新星吧，不然，在10多分的杂志上随便灌水，这个面子可是不小呀。
1.Gametogenesis in a dish [1422-1425]
Ying Gu1, Guang-Hui Liu1,2 and Juan Carlos Izpisua Belmonte1,3
2.The dawn of angiogenesis modeling: regenerating vasculature from human
pluripotent stem cells [3-5]
Fei Yi1,*, Jing Qu1,2,*, Guang-Hui Liu1,2 and Juan Carlos Izpisua Belmonte1,
3
3.Huntington's disease: Dancing in a dish [1627-1630]
Kejing Zhang1,*, Fei Yi1,*, Guang-Hui ... 阅读全帖 |
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s********3
发帖数: 231 | 41 《科学》对STAP手稿的专家评审意见曝光
《科学》对传说中的STAP细胞论文的三名评审专家意见，过去一直被雪藏，今天终于
被曝光。专家对这一研究的评价不高，认为非常全面的描述性研究，如果结果确定，可
能导致发育生物学大厦的颠覆，其实是不相信这种研究结论。因为没有当时的投稿件，
所以没有办法对这些评价进行更深入分析。不过这些资料显然都在某个地方，只是拥有
的人不愿意拿出来。
Retraction Watch readers are of course familiarwith the STAP stem cell saga,
which was punctuated by tragedy last month whenone of the authors of the
two now-retracted papers in Nature committed suicide.
In June, Science‘s news section reported:
Sources in the scientific community confirm that early version... 阅读全帖 |
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D*a
发帖数: 6830 | 42 什么干细胞有什么干细胞没有，这也不能一概而论啊
如果胚胎没有不对称分裂，那人生出来不还是一坨肉球啊？
要不就是定义成一个干细胞分出来一个progenitor，或者一个干细胞分出来两个
progenitor而已，我觉得这其实只是文字。
不过就是体外培养的时候罢了，但是细胞系那玩意真能研究生理功能？ |
|
g**********y
发帖数: 423 | 43 我的理解是，因为器官的细胞要频繁的更新，这个过程是progenitor cell转变成分化
的细胞，所以progenitor cell最容易癌变？ |
|
发帖数: 1 | 44 读了一下这篇文章，不喜欢这种研究思路，不能够称之为科学。
该实验室主页上面所挂出来的以下一些文章中，有很多引用率非常的低。
ROCKII inhibition promotes the maturation of human pancreatic beta-like
cells. Nature Communications. 2017 Academic Article GET IT
High-Content Screening in hPSC-Neural Progenitors Identifies Drug Candidates
that Inhibit Zika Virus Infection in Fetal-like Organoids and Adult Brain.
Cell Stem Cell. 2017 Academic Article GET IT
A Modular Platform for Differentiation of Human PSCs into All Major
Ectodermal Lineages. Cell Stem Cell. ... 阅读全帖 |
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s****e
发帖数: 7018 | 45 楼主是千老
发信人: todayxhli (), 信区: Biology
标 题: 求审稿机会：干细胞/心血管疾病/分子生物/再生医学/表观遗传
关键字: 申绿；审稿机会
发信站: BBS 未名空间站 (Wed Jan 25 05:09:22 2017, 美东)
本人申绿，急求Review稿件，已有一点经验。
在美有两年博后经历，主要研究方向：
1. Molecular/cellular biology.
2. Development of molecular/ cellular therapies for heart disease.
3. Stem and progenitor cell transplantation for cardiovascular regeneration.
4. Cardiovascular tissue engineering.
5. Development of novel methods of in vivo/ex vivo cell reprogram.
6. Novel approaches to therapeutic cell regen... 阅读全帖 |
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w*****g
发帖数: 1503 | 46 这种货色， 挫则挫矣，勇，大概他的菊花很勇吧
Yanzhong Wang
Lecturer in Medical Statistics
Background:
PhD in Statistics, University of Glasgow, 2000-2003.
Bioinformatician working on cancer genomics, University of Cambridge, 20
04-2006.
Statistician working on large epidemiological studies and clinical trial
s, Robertson Centre for Biostatistics, University of Glasgow, 2007-2009.
Research Interests:
Health Informatics, epidemiological studies and clinical trials;
Statistical genomics and genetics;... 阅读全帖 |
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p*****c
发帖数: 20445 | 47 Jun Lu PhD
Assistant Professor of Genetics
General Info
Bio
Research & Publications
Biographical Info
Education & Training
Ph.D.Boston University(2003)Postdoc AssociateBroad Institute or MIT and
Harvard, Cancer Program (2004 - 2008)
Research Summary
We are interested in using genomics to understand the role of non-coding
RNAs in mammalian development and disease. Currently, we are focusing on the
following topics. 1. Non-coding RNAs in blood stem cell differentiation and
malignancies 2. MicroRNA... 阅读全帖 |
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S*********g
发帖数: 24893 | 48 Yale的ＬＵＪＵＮ
Selected Publications
Jun Lu, Shangqin Guo, Benjamin L. Ebert, Hao Zhang, Xiao Peng, Jocelyn Bosco
, Jennifer Pretz, Rita Schlanger, Judy H. Wang, Raymond H. Mak, David M.
Dombkowski, Frederic I. Preffer, David T. Scadden and Todd R. Golub.
microRNA-mediated control of of cell fate in megakaryocyte-erythrocyte
progenitors. Developmental Cell, 14(6):843-53, 2008
Zejuan Li, Jun Lu (co-first author), Miao Sun, Shuangli Mi, Hao Zhang, Roger
T. Luo, Zhijian Qian, Mary Beth Neilly, Yungui W... 阅读全帖 |
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w****y
发帖数: 2952 | 49 有啊.他只列了几篇.
Jun Lu, Shangqin Guo, Benjamin L. Ebert, Hao Zhang, Xiao Peng, Jocelyn Bosco
, Jennifer Pretz, Rita Schlanger, Judy H. Wang, Raymond H. Mak, David M.
Dombkowski, Frederic I. Preffer, David T. Scadden and Todd R. Golub.
microRNA-mediated control of of cell fate in megakaryocyte-erythrocyte
progenitors. Developmental Cell, 14(6):843-53, 2008
Zejuan Li, Jun Lu (co-first author), Miao Sun, Shuangli Mi, Hao Zhang, Roger
T. Luo, Zhijian Qian, Mary Beth Neilly, Yungui Wang, Jie Jin, Yanming ... 阅读全帖 |
|
