m******5
发帖数: 1383 | 1 Gontan, C., T. Guttler, et al. (2009). "Exportin 4 mediates a novel nuclear
import pathway for Sox family transcription factors." J Cell Biol 185(1): 27
-34.
SRY and other Sox-type transcription factors are important developmental
regulators with various implications in human disease. In this study, we
identified Exp4 (exportin 4) as an interaction partner of Sox2 in mouse
embryonic stem cells and neural progenitors. We show that, besides its
established function in nuclear export, Exp4 acts |
|
s******y
发帖数: 28562 | 2 Your new serum is definitely suspicious then!
Call the company and ask them to send you an old batch, or ask around for
other labs to see if they have the old batch.
Or, order serum from a different company
By the way, don't take things too granted.
"真的进入differentiation，细胞在数量上应该不会增值才对吧" is not
theoretically or practically correct
For example, if your cells differentiated from stem cell into fibroblast, or
from stem cell into some kind of progenitor cells,
the differentiated cells surely will rep |
|
f********n
发帖数: 6465 | 3 Yu, Y.-C., Bultje, R.S., Wang X., and Shi, S.-H. (2009) Specific synapses
develop preferentially among sister excitatory neurons in the neocortex.
Nature 458: 501-504.
Wang, X., Tsai, J.-W., Imai, J.H., Lin, W.-N., Valle, R.B., and Shi, S.-H. (
2009) Asymmetric centrosome inheritance maintains neural progenitors in
neocortex. Nature 461: 947-955. |
|
p*****m
发帖数: 7030 | 4 就是说起源都不清楚？我只有个很简单的印象就是glia也从neural progenitor ce
ll来 中间有七七八八的fate determination factor参与。呵呵 我基本对发育问题
的细节知道的是零。。而且好像oligodendrocyte比astrocyte的发育搞得清楚些？ |
|
p*********8
发帖数: 1 | 5 Postdoctoral Position in Neural Stem Cell and Brain Tumorigenesis
A postdoctoral position is immediately available in the Zheng Laboratory at
Cold Spring Harbor Laboratory. This newly founded lab uses a range of in
vivo and in vitro approaches to study malignant gliomagenesis and its
relations to neural stem and progenitor lineage development (for more
information, please see - Cancer Cell 2010 17: 497; Nature 2008 455: 1129;
PNAS 2010 107: 6912). We are seeking a well-qualified and motivated
in |
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x*****a
发帖数: 972 | 6 我从血液中提取了一些progenitor cells，在flasks上养了几天后，冻成好几个vial。
这两周，
化了3个，分别做了flow，看其中CD105+的比例，结果3个vial的比例分别是55%，30%，
12%左右，
差别很大。
这种情况，算正常的么？
每次的操作都一样。 |
|
|
n**8
发帖数: 221 | 8 Title: Specification of Multipotential Cardiovascular Progenitor Cells
During Embryonic Stem Cell Differentiation and Embryonic Development
Journal: Trends in Cardiovascular Medicine, Volume 17, Issue 7, October
2007, Pages 240-246
Authors: Steven J. Kattman, Eric D. Adler and Gordon M. Keller
Hi, Daxia,
Could you please help me get the fulltext of this paper?
My email : d******[email protected]
Thank you very much!! |
|
t****p
发帖数: 1504 | 9 One example I heard is that if you inject ES cells instead of satellite
cells(muscle progenitor cell) to aged muscles, they can make younger muscle
cells. iPS should be able to do similar things. |
|
a******u
发帖数: 211 | 10 Aaron Yun Chen1, Elizabeth Yan Zhang1, Wuxiang Guan1, Fang Cheng1, Steve
Kleiboeker2, Thomas M. Yankee1, and Jianming Qiu1
The small 11kDa nonstructural protein of human parvovirus B19 plays a key
role in inducing apoptosis during B19 virus infection of primary erythroid
progenitor cells. Blood, 2010, 115(5): 1070
please send the paper to e*********[email protected] |
|
r***e
发帖数: 2539 | 11 你这个说法我不大认同。光能分裂应该不行吧，还要能分化成其他类型的细胞，才能说
stemness或者progenitor。
你说的是immortalized cell吧，大多数细胞株应该是。
你看下我原帖中的文章，也是说一部分细胞是stem cell like。 |
|
p******d
发帖数: 3737 | 12 I guess these are two different concepts. CSC is to discuss if there are
some unique cells in the tumor that can self-renewal and give rise to
other tumor cells. The question you raised here is about the cell origin
of tumor, which is related with CSC, however not the same concept.Take a
look at this pape:
Crypt stem cells as the cells-of-origin of intestinal cancer.
Barker N, Ridgway RA, van Es JH, van de Wetering M, Begthel H, van den
Born M, Danenberg E, Clarke AR, Sansom OJ, Clevers H.
Some ... 阅读全帖 |
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w********r
发帖数: 1431 | 13 我觉得是炒概念。
cancer progenitor cell可能是有的，
但是非要说就是stem cell，就感觉有点奇怪了
。当
li
也有
法是
enri
capacit
resistan |
|
t****p
发帖数: 1504 | 14 说到肿瘤的heterogeneicity，我来说点外行的想法。
就是说stem cell的概念不是绝对的，孤立的。所以人们引入了全能、多能的概念，又
引入progenitor,transient amplification cell的概念，目的是把连续的事件人为划
分为可描述的几个阶段。
相似的，在我看来找绝对概念的CSC是有问题的。一个肿瘤里面不应该只有两个细胞群
体，能重新形成肿瘤的csc和不能形成肿瘤的一般细胞，而是说每个细胞能形成肿瘤的
能力有高有低而已。所以，人们设想可以通过消灭一个肿瘤细胞中的特定细胞群来消灭
肿瘤的想法是误入歧途的。 |
|
l***m
发帖数: 26 | 15 读读吧：09 nature :Asymmetric centrosome inheritance maintains neural
progenitors in the neocortex.
读完就该知道了。 |
|
c****l
发帖数: 1086 | 16 问题同样在这里，就像正常组织一样，存在Stem cell (slow cycling)--> transit
amplifying cells (fast cycling progenitors) --> differentiated cells (non-
cycling) 这样hierarchy， 肿瘤组织中同样存在， 化疗药杀死的都是transit
amplifying cells 而不是stem cells。在这种情况下cancer stem cell的理论就具有
一定的意义了。 |
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D******t
发帖数: 79 | 17 I do not know either cells you mentioned, but can every 神经纤维瘤细胞 and
黑痣细胞 divide? I did not say "能分
裂的肿瘤细胞就算肿瘤干细胞", as upstairs says stem cell differentiation is a
hierarchy process. The cells that still
can divide can be one of the transit progenitors. |
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b****r
发帖数: 17995 | 18 问题是肿瘤里一定就是Stem cell (slow cycling)--> transit : amplifying cells (
fast cycling progenitors) 这样一种模式吗。为什么不可以是这么一种模式，类似于
在体外培养耐药菌：
培养皿上种了一个细菌后，它变成一群细菌，每个个体在以同样的速度突变和繁殖，外
来因素如抗叶酸药物磺胺进来后，绝大部分细菌死了，只有偶然突变出耐药性的细菌生
存下来，并且繁殖开来成为relapse的源泉，而且relapse的细胞对原有的化疗相对耐药。
现在所谓CSC而不是single clone expansion的主要证据，如果我理解正确，是数个
clone的expansion而不是细菌常见的单个clone expansion，但是我以为这个非常好理
解，因为化疗药物对肿瘤细胞的杀伤能力基本上是远远逊于抗生素对细菌的杀灭能力的
（因为化疗药物并没有办法真正区分肿瘤细胞和正常繁殖的细胞），所以实际上只是给
肿瘤细胞一个较长周期的外界压力，因而肿瘤细胞有机会在这么长一段时间里以多种不
同的方式进化出抗肿瘤的能力。类似于如果只是以较低浓度... 阅读全帖 |
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b****r
发帖数: 17995 | 19 神经纤维瘤细胞和 黑痣细胞在刚开始成瘤的那个阶段是每个细胞都在繁殖的，但是那
个时候你同样不能把他们都称作CSC，或者transit progenitors，对吧？我只是在用一
个极端的例子反驳他
and
a |
|
Z**********g
发帖数: 222 | 20 1. 关于cell (slow cycling)--> transit : amplifying : cells--> differentiated
cell,在正常组织中研究比较深入的比如intestinal stem cells,文献参阅:
Nature. 2010 Oct 28;467(7319):1055-6.
在肿瘤组织中,证据确实比较少,但也有,参见
Cell. 2009 Sep 18;138(6):1083-95.
2. 看了你的所有帖子,感觉你的观点其实就是clonal evolution.但是我觉得你把
clonal selection和CSC绝对对立了.在肿瘤起始阶段,clonal evolution当然是起作用
的,足够的一系列突变导致某个细胞被彻底恶性转化,这个被转化的肿瘤起始细胞可以认
为是肿瘤的始祖,可能是CSC,也可能不是.如果该起始细胞是CSC,那么它就会发生self-
renewal和differentiation;在CSC self-renewal中,如果对称分裂,那么CSC就开始
expansion了,而非对称分裂,那么就会产生progenitor... 阅读全帖 |
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a****d
发帖数: 1919 | 21 Within few years, scientists should be able to convert fell fates as they
wish. |
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n********k
发帖数: 2818 | 22 the iPS hypo still not done yet...although researches along this line are
very much valuable...I think the next going to be direct F- to any without
any factors.... |
|
b*****l
发帖数: 9499 | 23 MEF 的 piPS 已经做成了吧。
they |
|
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a****d
发帖数: 1919 | 25 You are right, iPSCs hypo is still there. It's just such a crowded field now
and people are going nuts to compete for publishing.People from out lab
just found almost identical xx cell derived iPCSs results being published.
Those disease iPSCs models will continue to water on top journals for sure.
Along with that, reprogramming becomes hotter and hotter. For the next few
years, people still have to rely on TFs for reprogramming. Large small
molecular screening is on the way to replace those TFs... 阅读全帖 |
|
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D*a
发帖数: 6830 | 27 就看了个文章题目，multilineage blood progenitors不就可以治白血病么？ |
|
a****d
发帖数: 1919 | 28 My two points of view:
Most organ development happened along with angiogenesis. Within developing
tissue, the multi-potent cells/progenitors are normally close to vasculature
, while those more differentiated cells are away from the vessles.The
developing tissue always secreted angiogenesis cues like VEGF, and the
growing vasculature helped to further pattern the tissue in a feedback way.
As to your question, normal organ development should be impaired in hypoxia
environment.But during normal or... 阅读全帖 |
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a****d
发帖数: 1919 | 29 I am not working on EMT,so don't take my words too seriously.
In adulthood, within certain organs, stem cells/progenitors proliferate and
differentiate to maintain homeostasis. It looks like what happened during
this homeostasis maintenance, are utilizing similar group of EMT related
genes.I am not saying the stem cells behave like "M",or the differentiated
cells like"E".I just thought similar groups of genes were involved in
homeostasis.
Morphin said EMT was de-differentiation, and I do agree o... 阅读全帖 |
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z****g
发帖数: 3340 | 30 Pubmed Pierre Chambon with key words RA neuron. |
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o**i
发帖数: 1165 | 31 貌似这种protocol都本主都不会拿出来share |
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h******y
发帖数: 351 | 32 Proliferation of Hepatocyte Progenitor Cells Isolated From Adult Human
Livers in Serum-Free Medium
Authors: Sasaki, Kazunori; Kon, Junko; Mizuguchi, Toru; Chen, Qijie; Ooe,
Hidekazu; Oshima, Hideki; Hirata, Koichi; Mitaka, Toshihiro
Source: Cell Transplantation, Volume 17, Numbers 10-11, 2008 , pp. 1221-1230
(10)
http://openurl.ingenta.com/content/nlm?genre=article&issn=0963-
Please send it to z*****[email protected]
Thank you very much for your help! |
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m*p
发帖数: 226 | 33 Fibroblast are everywhere or in every organ, skin, heart, liver, etc.
Fibroblast are a type of mesenchymal cell, which are usually derived from
the epithelial-mesenchymal transition process. It seems fibroblast can be
derived from ectoderm, mesoderm, and endoderm. Fibroblast synthesize ECM and
collagen, and are important for organ structure (my personal two cents).
Myo-blast are myocyte progenitors. |
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c****l
发帖数: 1086 | 34 不好意思，没有暴露你的意思，也没有人肉的兴趣，
我只是本身对PDGFRA的功能，以及MSC 二者都感兴趣，这个paper刚刚出来的时候很是
兴奋了一阵，我们主要是对其在脑子里的功能感兴趣，PDGFRA在脑子里比较特异的在
oligodendrocytes progenitor cells 里表达， 在glioblastoma里有amplification，
所以我比较关注。
Philippe Soriano很牛的。有前途！ |
|
c****l
发帖数: 1086 | 35 不好意思，没有暴露你的意思，也没有人肉的兴趣，
我只是本身对PDGFRA的功能，以及MSC 二者都感兴趣，这个paper刚刚出来的时候很是
兴奋了一阵，我们主要是对其在脑子里的功能感兴趣，PDGFRA在脑子里比较特异的在
oligodendrocytes progenitor cells 里表达， 在glioblastoma里有amplification，
所以我比较关注。
Philippe Soriano很牛的。有前途！ |
|
D******t
发帖数: 79 | 36 做progenitor 分化的。在纽约洲中部。有兴趣的先站内联系，在细谈。 |
|
j*****d
发帖数: 787 | 37 个人感觉算不上太意外
饶毅都证明了基因本突变改变性倾向
动物模型太重要了，别人重复你的东西也有一个平台了，而且是sustainable的
这几天的ARVO回忆有三个特点：imaging, stem cell/progenitor cell/cell
replacement/gene replacement,最后一个就是动物疾病模型指数增加。 |
|
m******5
发帖数: 1383 | 38 I don't think it is necessary a role related to immune system: for example,
cxcl-cxcr are important for germ cell migration during development, and
sometimes cxcls are directly related to migration of progenitor cell such us
osteoblast .
But I am still feeling that I am missing a big picture…… which makes me
very uncomfortable
you |
|
c********r
发帖数: 1125 | 39 这个chemokine CXCL family factors在发育里面的作用不是什么很新的东西了，在PGC
迁移，肌肉前体细胞迁移，血管发生上面都有报道了。
我记得比较清楚的是在斑马鱼里面的工作，你可以参考:
Siekmann, A.F., Standley, C., Fogarty, K.E., Wolfe, S.A., Lawson, N.D. (2009
): Chemokine signaling guides regional patterning of the first embryonic
artery. Genes & Development, 1;23(19): 2272-7.
Vasyutina, E., Stebler, J., Brand-Saberi, B., Schulz, S., Raz, E. and
Birchmeier, C. (2005)
CXCR4 and Gab1 cooperate to control the development of migrating muscle
progenitor cells.
Genes and Devel... 阅读全帖 |
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D*a
发帖数: 6830 | 40 Fluorescence-based sorting of neural stem cells and progenitors.
Maric D, Barker JL.
Curr Protoc Neurosci. 2005 Nov;Chapter 3:Unit 3.18
多谢! |
|
s********n
发帖数: 342 | 41 想请教一个问题，
如果把免疫细胞全部干掉，再重新恢复的免疫细胞。
这个有点类似于regeneration，可是有点不清楚的就是，
这个regeneration的过程和免疫细胞原初发育的过程是不是一样，
具体就是那些stem cell以及progenitor的marker什么的是不是一样，还有就是发育的
过程是不是一样。如果有哪位大牛懂得比较多，请推荐几篇研究免疫细胞在病人
reconstitution的文章，
谢谢！
看到很多在免疫里面在人和老鼠里面完全相反的结果，
最近又看到那些所谓的stem cell的marker完全不一样，
真是不理解这些免疫的研究为什么要投入那么大的精力到老鼠里面去做？ |
|
e****p
发帖数: 354 | 42 progenitor cell 和 cancer cell 会不会有 co-translational degradation pathway |
|
s********n
发帖数: 342 | 43 觉得还是单开一个话题比较好。
想请教一个问题，
如果把免疫细胞全部干掉，再重新恢复的免疫细胞。
这个有点类似于regeneration，可是有点不清楚的就是，
这个regeneration的过程和免疫细胞原初发育的过程是不是一样，
具体就是那些stem cell以及progenitor的marker什么的是不是一样，还有就是发育的
过程是不是一样。如果有哪位大牛懂得比较多，请推荐几篇研究免疫细胞在病人
reconstitution的文章，
谢谢！
看到很多在免疫里面在人和老鼠里面完全相反的结果，
最近又看到那些所谓的stem cell的marker完全不一样，
真是不理解这些免疫的研究为什么要投入那么大的精力到老鼠里面去做？ |
|
a********k
发帖数: 2273 | 44 花了一个小时，深深的鄙视一下自己的无聊行径！！
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branches in lamellipodia. Cell. 2008 Sep
5;134(5):828-42. PubMed PMID: 18775315; PubMed Central PMCID: PMC2570342.
Cai L, Makhov AM, Bear JE. F-actin... 阅读全帖 |
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B*****e
发帖数: 1005 | 45 140后的几个也不是美国AP啊，文章比跟前几个有距离
140 黄亿华 男 1971年10月 中国科学院生物物理研究所 生命
科学 2004年02月毕业于[美国]康奈尔大学 [美国]休斯医学研究所/西南医学中
心 讲师
1: Huang Y, Smith BS, Chen LX, Baxter RH, Deisenhofer J. Insights into pilus
assembly and secretion from the structure and functional characterization of
usher PapC. Proc Natl Acad Sci U S A. 2009 May 5;106(18):7403-7. Epub 2009
Apr
20. PubMed PMID: 19380723; PubMed Central PMCID: PMC2670885.
2: Lin SC, Huang Y, Lo YC, Lu M, Wu H. Crystal structure of the BIR1 doma... 阅读全帖 |
|
m*****u
发帖数: 15526 | 46 血液细胞很多都有游走，贴壁的特性。用96孔板，液体培养基最后怎么收集分析？用半
固体培养基的重要作用是可以观察血细胞集落，有经验的根据集落形态，颜色，大小就
可以判断progenitor的分化情况。细胞集落也容易收集分析，验证 |
|
f********s
发帖数: 115 | 47 96孔板每一个孔就是200ul 培养基，最后把这200ul全部吸出来，细胞全部离心到slide
上面，然后染色就可以看到子细胞的形态。在这种情况下，不靠细胞集落的形态分析，
直接看每一个细胞的形态，根据形态可以判断子细胞的类型。
根据细胞集落的形态，颜色，大小来判断progenitor的分化对于wildtype还比较可靠，
对于mutant来说不可靠，各种不正常都可能发生。最后还是需要挑单克隆，看细胞来进
一步验证。 |
|
m*****u
发帖数: 15526 | 48 半固体培基主要目的是估计你的细胞样本中colony forming cells 的数量，功能和类
型。无论你用PBMC还是CD34,能形成colony的细胞只是其中一部分。根据colony的数量
，形态，大小，颜色可以知道血细胞红系，粒系，单核巨噬各系progenitor的分布和
colony形成能力。在液体培养中，所有分化的lineage都mix一起，和不能形成colony的细
胞也混在一起，这样这些就都无法判断。 |
|
n********k
发帖数: 2818 | 49 there were two science papers, one was on human brain stem cells using
lipids as a stem cell/proliferation marker. I thought it is very cool and
has tremendous potentials but I have no expertise on the subject whatsoever
and cannot really comment on it...
Magnetic Resonance Spectroscopy Identifies Neural Progenitor Cells in the
Live Human Brain |
|
n********k
发帖数: 2818 | 50 This is a smart, very focused and productive one I have heard from people
working with him...XD Wang's first student was even more impressive if I am not too old:) and several from HJ Song's lab also have impressive records, A and P from this board both have impressive records in pretty shoret time too...
BTW, to upbeat/Q a bit for those (including myself) who may feel depressed now)....Nothing particularly/unusually, Pretty much decent intelligence, strong motivations/working/person habits/... 阅读全帖 |
|
