s******s
发帖数: 13035 | 1 不是imprinting, x-inactivation一类的？我就不知道了 |
|
k*****n
发帖数: 417 | 2 Their top5 highly cited research papers:
Wei Gu:
Title: Negative control of p53 by Sir2 alpha promotes cell survival under
stress
Times Cited: 822 (from Web of Science)
Title: Deacetylation of p53 modulates its effect on cell growth and
apoptosis
Times Cited: 331 (from Web of Science)
Title: Deubiquitination of p53 by HAUSP is an important pathway for p53
stabilization
Times Cited: 319 (from Web of Science)
Title: Mono-versus polyubiquitination: Differential control of p53 fate by
Mdm2
Times Ci... 阅读全帖 |
|
d*****r
发帖数: 2583 | 3 http://www.hhmi.org/news/popups/isrf_pop.html
2011 International Student Research Fellows
Institution Student Advisor Discipline
Boston University Wei Guo
China Barbara Shinn-Cunningham Systems and computational
neuroscience
Brandeis University Marcus Long
United Kingdom Lizbeth Hedstrom Protein degradation using chemical
biology
California Institute of Technology Andreas Hoenselaar
Germany Thanos Siapas Spatial mapping in the hippocampus using
virtual
reality... 阅读全帖 |
|
c****l
发帖数: 1086 | 4 Lots of membrane amino acid transporters has been identified!! You can
easily find a review paper on this like the following one.
Amino Acid Transport Across Mammalian Intestinal and Renal Epithelia
http://physrev.physiology.org/content/88/1/249.short
The transport of amino acids in kidney and intestine is critical for the
supply of amino acids to all tissues and the homeostasis of plasma amino
acid levels. This is illustrated by a number of inherited disorders
affecting amino acid transport in ... 阅读全帖 |
|
c****l
发帖数: 1086 | 5 Lots of membrane amino acid transporters has been identified!! You can
easily find a review paper on this like the following one.
Amino Acid Transport Across Mammalian Intestinal and Renal Epithelia
http://physrev.physiology.org/content/88/1/249.short
The transport of amino acids in kidney and intestine is critical for the
supply of amino acids to all tissues and the homeostasis of plasma amino
acid levels. This is illustrated by a number of inherited disorders
affecting amino acid transport in ... 阅读全帖 |
|
a********k
发帖数: 2273 | 6 花了一个小时，深深的鄙视一下自己的无聊行径！！
125 蔡亮 男 1980年11月 复旦大学 生命
科学 2007年12月毕业于[美国]北卡大学 [美国]加州大学旧金山分校 博士后
Cai L, Mostov K. Polarity is destiny. Cell. 2009 Nov 13;139(4):660-2. PubMed
PMID: 19914162; PubMed Central PMCID: PMC2900917.
Cai L, Makhov AM, Schafer DA, Bear JE. Coronin 1B antagonizes cortactin and
remodels Arp2/3-containing actin branches in lamellipodia. Cell. 2008 Sep
5;134(5):828-42. PubMed PMID: 18775315; PubMed Central PMCID: PMC2570342.
Cai L, Makhov AM, Bear JE. F-actin... 阅读全帖 |
|
z*t
发帖数: 863 | 7 Beutler得奖，Medzhitov会不会有意见？
http://www.sciencedirect.com/science/article/pii/S1074761309002
Ruslan Medzhitov
This year marks the 20th anniversary of a publication (Janeway, 1989) that
in many ways revolutionized our understanding of the immune system. As part
of the proceedings of the Cold Spring Harbor Symposium on Immune Recognition
, the paper authored by the late Charles A. Janeway, Jr. was not a standard
peer-reviewed publication. In fact, Charlie used to refer to it as “the
best paper I've ... 阅读全帖 |
|
j******2
发帖数: 544 | 8 昨晚离开实验室前配了media，用了3.5支FBS，并在实验室白板上写明了，需要再
aliquot。由于配media取用FBS的时候正好被T看到，我当时心就抽了一下，有不详的预
感。T是负责aliquot FBS的人。晚上收到T的邮件，发给实验室所有人，如下：
People,
We seriously need to work on communicating more effectively on things that
need thawing time and aliquoting, like the FBS. It just so happened that I
looked yesterday before I left the lab at the FBS aliquots and we had 5. I
took out FBS to thaw this morning and right now at 6pm we are down to 1
aliquot. We are going through medium like crazy, which means ... 阅读全帖 |
|
O******e
发帖数: 4845 | 9 我已经跟他们谈到$148/500mL了，Characterized, US Origin, Heat Inactivated。
是不是还是太贵？ |
|
b**********8
发帖数: 349 | 10 最近开始做这个实验，大概流程如下，RSB+0.5% NP-40 分离nuclei，然后用NEB 公司
的 DNase I （浓度分别为0,8,16,32,64,128 Units/ml）在37度切25min，加EDTA(
final conc 5mM)混合后75度10min终止反应，蛋白酶k 56度过夜消化，酚氯仿抽提。取
2ug 基因组DNA跑0.8% TBE 胶，理论上随着DNase I 酶量加大，应该能看到一个DNA逐
渐变碎的过程，可是我做了几次，都只看到一个很微弱的趋势，我现在有两个问题想请
教大家，
1）是否一定要在DNase I 处理后看到一个明显的基因组DNA变碎的趋势？
2）NEB网站上建议的DNase I处理完毕后需要加一定量的的EDTA，再75度10min，但是我
看别的文献里说EDTA是用来保护mRNA不受降解的，适用于做reverse transcription之
前去处RNA样品中残留的基因组DNA。我这个实验里需呀尽量除去RNA，是否可以不加
EDTA而直接做heat inactivation？
请大家帮助，谢谢 |
|
g***j
发帖数: 40861 | 11 s*****[email protected]
谢谢！
1.
Inactivation of Escherichia coli O157:H7 and Salmonella Typhimurium DT 104
on Alfalfa Seeds by Levulinic Acid and Sodium Dodecyl Sulfate
Authors: Zhao, Tong; Zhao, Ping; Doyle, Michael P.
Source: Journal of Food Protection®, Volume 73, Number 11, November
2010 , pp. 2010-2017(8)
2.
Control of Salmonella on Sprouting Mung Bean and Alfalfa Seeds by Using a
Biocontrol Preparation Based on Antagonistic Bacteria and Lytic
Bacteriophages
Authors: Ye, Jianxiong1; Kostrzyns... 阅读全帖 |
|
t*********1
发帖数: 418 | 12 有没有那位同学知道 "ChR2 variant with prolonged inactivation kinetics"?
Is ChR2(H134R)a good example?
Thanks! |
|
h******t
发帖数: 56 | 13 对了，忘记说了。我用的是RPMI+10% heat inactivated FBS+PEN/STREP和50ng/ml GM-
CSF |
|
i**y
发帖数: 71 | 14 Many enzymes lose activity upon heat and cannot refold without chaperones.
Lysozyme, alcohol dehydrogenase are among some of the commonly used models.
I measured heat inactivation of trehalase when I was in college, also very
easy. |
|
n***w
发帖数: 2405 | 15 DirectPCR lysis reagent (Viagen Biotech 102-T) and proteinase K (Viagen
Biotech 501-PK).
100ul lysis reagent + 2.5ul proteinase K, 55 degree O/N (>2hrs or more), 95
degree inactivate 15min
ready to go.
Sometimes you may need to consider DNA purification. |
|
w**********s
发帖数: 72 | 16 Thanks so much!
Biochim Biophys Acta. 2012 Jun 21. [Epub ahead of print]
Triacylglycerol-rich lipoproteins protect lipoprotein lipase from
inactivation by ANGPTL3 and ANGPTL4.
Nilsson SK, Anderson F, Ericsson M, Larsson M, Makoveichuk E, Lookene A,
Heeren J, Olivecrona G.
SourceDepartment of Medical Biosciences/Physiological Chemistry, Umeå
University, SE-901 87 Umeå, Sweden. |
|
r***y
发帖数: 25 | 17 It is a quite established field for both neuroscience and engineering
research now. The fundamental was paved by Apostolos P. Georgopoulos (U
Minnesota, http://www.neurosci.umn.edu/faculty/georgopoulos.html) in their papers published in early 1980's about population coding of movement direction in Monkey's motor cortex. Another breakthrough was made by Miguel Nicolelis (Duke U, http://www.nicolelislab.net/) in early 2000's -- manipulate robotic arms/effectors using multi-unit recording in mice a... 阅读全帖 |
|
l**********1
发帖数: 5204 | 18 Mulet X et al.
Antagonistic interactions of Pseudomonas aeruginosa antibiotic resistance
mechanisms in planktonic but not biofilm growth.
Antimicrob Agents Chemother. 55: 4560-8.
Abstract
Pseudomonas aeruginosa has an extraordinary capacity to evade the activity
of antibiotics through a complex interplay of intrinsic and mutation-driven
resistance pathways, which are, unfortunately, often additive or synergistic
, leading to multidrug (or even pandrug) resistance. However, we show that
one of th... 阅读全帖 |
|
v***a
发帖数: 1242 | 19 在做一个作用广泛的protein kinase在某相对封闭的生物系统中的作用。这个kinase在
N多biological functions中都神通广大，可以激活很多的信号通路，还可以recruit很
多的蛋白（包括其它kinase和膜蛋白）。关于这个蛋白，我现在的lab已经做了很多工
作，有好几篇paper。现在老板叫我继续做，他的想法是把这个蛋白knock down，还有
用site-directed mutagenesis搞一个constitutive activated/inactivated form。但
这个方法已经被lab做其它protien的，还有做相似kinase的用烂了。而且我想这个蛋白
本身功能广泛，肯定会牵动很多的通路，最后的变化反而不好确定，解释起来也复杂多
多。
想听听大家的想法？还有其它研究的好点子吗。谢谢～～ |
|
s**x
发帖数: 17 | 20 a new one
http://www.cell.com/abstract/S0092-8674%2812%2900941-5
Structurally Distinct Bacterial TBC-like GAPs Link Arf GTPase to Rab1
Inactivation to Counteract Host Defenses
Cell, Volume 150, Issue 5, 1029-1041, 31 August 2012
Na Dong, Yongqun Zhu, Qiuhe Lu, Liyan Hu, Yuqing Zheng, Feng Shao |
|
l*******i
发帖数: 153 | 21 首先有一个概念要明确：目前说的ES细胞，都是in vitro culture的，与in vivo的不
一样。
ES细胞分为naive和prime两个stage
1)naive stage：ES colony在形态上看起来更“致密”，呈三维结构，对胰酶不敏感（
即胰酶消化不会诱导其凋亡）。对小鼠ES而言，naive的ES来源于inner cell mass(ICM
)。此stage的ES self-renewal更迅速，population double大概只需要12-14小时。对
于其self-renewal的维持需要LIF，stat3,BMP4等。雌性小鼠naive ES的另一个显著特
点是X chromosome reactivation,而在其他类型的细胞中有一条X chromosome是失活的
。从全能性角度来讲，naive ES是真正具有全能性的，能形成完整的个体。
2）prime stage：对小鼠而言，其来源于postimplantation epiblast,是naive stage
的下一个阶段，其形态看起来与naive ES明显不一样，呈“二维”结构，即colony... 阅读全帖 |
|
l**********1
发帖数: 5204 | 22 RE LZ:
pls try
Cassette-ligation downstream 3'RACE PCR
or Tail-PCR
Protocol:
看图说话
>
Amplification of FSTs by 3’-RACE
Total RNA was isolated using hot-phenol extraction
[27], from 20 ml cultures in 50 ml Falcon tubes on a rotating
wheel. Reverse transcription used the SuperScript
III First-Strand kit from Invitrogen in a DNA Engine
PCR machine (MJ research). Primer QT was mixed with
5 μg RNA, incubated at 65 °C for 5 min then cooled to
55 °C over 100 sec, after which the annealed mixture
was kept... 阅读全帖 |
|
e**e
发帖数: 166 | 23 Targeted inactivation of transcription factors by overexpression of their
truncated forms in plants
Plant J 7: 162–172
Pil Joon Seo, Shin-Young Hong, Jae Yong Ryu, Eun-Young Jeong, Sang-Gyu Kim,
Ian T. Baldwin and Chung-Mo Park
please send to z******[email protected]
thanks a lot |
|
m******5
发帖数: 1383 | 24 1, It really depends on whether your inserted location should be activated
in undifferentiated mES cells. If not, you have to either make embryoid body
differentiation or take a leap of faith waiting for your mice.
2, CMV promoter does activate in undifferentiated mES cells, it is only
inactivated after several rounds of proliferation and differentiation.
However, CAGGS promoter is much safer, you could easily find one on Addgene
3,!!!! And, whatever kind of transfection methods you use, wheneve... 阅读全帖 |
|
b*******6
发帖数: 39 | 25 乙肝在中国大规模传播的原因可能有3点原因：
公用针头免疫疫苗以及未检测乙肝的输血，母婴传播
乙肝特性：
HBV病毒很古老，恐龙时代即存在（ref1）
在16世纪韩国人木乃伊上检测到HBV（ref2）
乙肝可以通过血液非常高效的传播，CID50：10 copy（ref3，6）
HBV病毒相当耐受加热（ref4）
乙肝是在1964年发现的，输血很早就用于临床
临床输血的血液大规模常规检查HBV之前，肯定会广泛加速HBV传播
值得一提的是，血液elisa检测并不能彻底排除HBV（HBsAg突变）
只有高灵敏度PCR检测HBV的DNA才算比较可靠的排除血液不含有HBV病毒
但是成年人输血，猜测如果血液中copy数不高的话会大部分自愈？（ref？）
但是欧美国家同样也未检测HBV输血，流行率2%左右而不是中国的9%左右
之间7%差值的流行可能因为公用针头免疫疫苗
解放后中国对卫生事业的重视，大规模对儿童免疫（时间ref？），
但是很遗憾的是，相当一段时间公用针头，公用针头免疫会造成对HBV的传播（ref？）
婴幼儿童对HBV相当易感，婴幼儿童被HBV感染后，大部分终生携带，不能自我清除
可能因此... 阅读全帖 |
|
l**********1
发帖数: 5204 | 26 Re LZ:
try Stem Cell line: H9 hESCs
cited from
Das B. et al. (2012)
HIF-2a Suppresses p53 to Enhance the Stemness and Regenerative
Potential of Human Embryonic Stem Cells.
Stem Cells. 30: 1685-95.
>
hESC Culture and Induction of Hypoxia
Due permissions were obtained to culture hESCs both at Department
of Medicine, Stanford University (Stem Cell Research
Oversight protocol #285, director: BD) and at Department of
Laboratory Medicine and Pathobiology, Hospital for Sick Children.
BGO1 and H9 hESCs... 阅读全帖 |
|
l**********1
发帖数: 5204 | 27 不用谢
Li D et al. (2011)
HSP90 Causes Aberrant Stabilization of Mutant p53 in
Functional Inactivation of Endogenous MDM2 and CHIP by human cancer cells
Mol Cancer Res. 9: 577–588.
HTTP: //www.ncbi.nlm.nih.gov/pmc/articles/PMC3097033/
plus
参看艳阳天jj/mm 的原帖下吧
昨天晚上听了Susan Lindquist 有关HSP90 的讲座后被震惊了。虽然以前这里也有人提
起过她以前的文章，但是那些文章都是一些计算分析为主的，所以我也比较不怎么当一
回事。昨天她谈了她们做的一些关于防止真菌进化出抗药性的实验，HSP90
抑制剂对于真菌没有直接杀伤力，但是综合和其他有直接杀伤力的药一起用的时候可以
非常好的防止真菌出现抗药性而复发。她最后的结论是HSP90的活性对于容忍机体的进
化有重要作用。高活性的HSP90会导致突变体能够成功存活下来。听完之后我忍不住跑... 阅读全帖 |
|
r**o
发帖数: 212 | 28 FASEB J. 2012 Aug;26(8):3140-7. doi: 10.1096/fj.11-198515. Epub 2012 Apr 24.
MicroRNA-31 targets FIH-1 to positively regulate corneal epithelial glycogen
metabolism.
Peng H, Hamanaka RB, Katsnelson J, Hao LL, Yang W, Chandel NS, Lavker RM.
Corneal epithelium relies on abundant glycogen stores as its primary energy
source. MicroRNA-31 (miR-31), a corneal epithelial-preferred miRNA,
negatively regulates factor inhibiting hypoxia-inducible factor-1 (FIH-1).
Since HIF-1α is involved in anaerobic ene... 阅读全帖 |
|
M*S
发帖数: 5 | 29 我大概年底毕业，想试试到她的实验室（Harvard medical school）找薄厚的位置。因
为对她们实验室的方向比较感兴趣（x-chromosome inactivation and lncRNAs)。不知
道有没有人了解她？她们实验室的氛围如何？谢谢 |
|
l**********1
发帖数: 5204 | 30 >发信人: MGS (Snake), 信区: Biology
标 题: Re: 打听一下Jeannie Lee实验室的情况
发信站: BBS 未名空间站 (Thu May 2 10:23:45 2013, 美东)
谢谢啊，
请问有人了解她的实验室内部有竞争么？
don't know that
but her talk Stream link:
http://www.imedseminar.com/seminars/streamed?page=2
title:
Lessons from X-Chromosome Inactivation: RNA, Chromatin, and Stem Cells
or her speech at in Boston for the
first conference on Epigenetics & Chromatin: interactions and processes
from 11-13 March 2013 by BioMed Central
http://www.epigeneticsandchromatin.com/conferenc... 阅读全帖 |
|
s******y
发帖数: 28562 | 31 我听说有些公司的lentivirus vector 久了就会被inactivated |
|
d*****i
发帖数: 3905 | 32 mRNA跟WB cell大概早了两个passag。被inactivate mRNA level不会回去？只在
protein level？ |
|
k****s
发帖数: 1209 | 33 原文在
http://www.skizit.biz/2013/07/21/ecdysone-insect-hormone-an-ind
Ecdysone Insect Hormone Switches on Disease
21 Jul 2013 | ECDYSONE
MORGELLONS:
INSECT HORMONE ECDYSONE TRIGGERS GENETIC CHANGES
TO BUILD NEW HAIR, SKIN, NAILS and CAUSE DISEASES
SkizitGesture-2013
Morgellons is a military grade entomological terror weapon being used to
torture and kill innocent civilians. The medical community has not been
informed about this disease and its complexity keeps it from being properly
identified, ... 阅读全帖 |
|
T**********t
发帖数: 1604 | 34 DNase I不会degrade RNA。你有RNA degradation是因为你的sample有RNase污染。
DNase I处理之后跑胶纯化就不用管heat inactivation了，就是麻烦点。 |
|
|
i*********0
发帖数: 915 | 36 我明天确认一下是不是female 的ESCs。
对于其他的问题：
几个问题：
3) mESC的培养条件？serum,还是2i/LIF?
serum + LIF的培养条件
1）knockdown的mESC是否仍保持未分化状态，还是开始分化了？如果未分化，那么很有
可能你发现了一个新的调节X reactivation/inactivation的因子，做深了，至少是
cell stem cell.如果细胞分化了，那么X chromosome上的genes表达下调不奇怪，有多
大新意取决于你的这个gene
如果要看Oct4等gene的表达的话，RNA 水平上看到降低，Protein水平上有20%左右的降
低。但是这些pluripotency的gene在蛋白水平还维持在一个高的状态。
2）看一下这个基因的功能，是否已有先关报道，与X chromosome reactivation有关。
是histone modifier, TF还是在X chromsome上的新lncRNA?
该gene目前的功能还不清楚。另外，我看到Xist gene在shRNA处理想样品中，在RNA水
平上有3倍数的降低。 |
|
c*********r
发帖数: 1312 | 37 One-step inactivation of chromosomal genes in Escherichia coli K-12 using
PCR products
By: Datsenko, KA; Wanner, BL
被引用了500多次的方法了。我本科就靠它，没有电转化，只用钙转，每边好像是25bp
还是50bp同源序列来着，敲除了好几个基因，得意了好一阵子呢。 |
|
x****s
发帖数: 29 | 38 I think it means the pathway is not working normally as it is supposed to be
. In normal cells, numerous cellular pathways are being precisely regulated
to ensure a normal functional output. However, in the case of cancer, the
situation of pathways is usually found quite different. For example, p53
inactivation is frequently observed in many cancer types. This molecule is
critical in DNA repair, cell cycle as well as apoptosis of host cells.
Therefore, abnormality of this molecule would cause an... 阅读全帖 |
|
r******k
发帖数: 446 | 39 小弟最近看了看糖尿病的research。 发觉糖尿病人都是insulin resistance，然后
PI2k pathway是inactivation的。 那这不是跟癌症的pi3k pathway activation正相反？
而且如果治疗糖尿病的话，restore PI3k activation那不是很容易得癌症吗？
小弟不懂 忘大神们指点一二！ 谢谢！ |
|
i******n
发帖数: 181 | 40 跟metaformin服用有相关性吗？好像听过报告metaformin 减少癌症。
小弟最近看了看糖尿病的research。 发觉糖尿病人都是insulin resistance，然后
PI2k pathway是inactivation的。 那这不是跟癌症的pi........ |
|
r******k
发帖数: 446 | 41 小弟最近看了看糖尿病的research。 发觉糖尿病人都是insulin resistance，然后
PI2k pathway是inactivation的。 那这不是跟癌症的pi3k pathway activation正相反？
而且如果治疗糖尿病的话，restore PI3k activation那不是很容易得癌症吗？
小弟不懂 忘大神们指点一二！ 谢谢！ |
|
i******n
发帖数: 181 | 42 跟metaformin服用有相关性吗？好像听过报告metaformin 减少癌症。
小弟最近看了看糖尿病的research。 发觉糖尿病人都是insulin resistance，然后
PI2k pathway是inactivation的。 那这不是跟癌症的pi........ |
|
b*******1
发帖数: 16 | 43 岗位一：吴虹课题组博士后工作人员 （2名）
主要职责：
1）独立开展科研工作，动手进行体内外实验；
2）协助PI负责实验室研究课题的设计和开展；
3）参与科研基金撰写；
4）协助培养研究生。
招聘条件：
1）品学兼优，身体健康，年龄在40岁以下；
2）近年来在国内外获得分子生物学、细胞生物学、肿瘤遗传学、药理学等相关专业博
士学位；
3）至少具备一项以下领域内的工作经验和文献发表：
a. 独立运用细胞和动物模型进行肿瘤药物的机理研究
b. 在分子生物学和细胞生物学领域有深厚的知识背景；
c. 在蛋白质分析、核酸分析和细胞生长分析等相关技术方面有丰富的经验；
d. 对治疗癌症的药物疗效和安全性评价方面有强烈的兴趣和研究背景 ；
4）有较强的团队合作精神，沟通协调能力强，动手能力强，工作细心，积极主动，能
持之以恒；
聘用待遇：
本职位将为你提供在生物学领域极具竞争力和创造性的工作机会。博士后工作人员薪酬
标准按照北京大学生命科学院和北大-清华生命科学中心相应规定及个人工作经验及能
力确定。
联系方式：
有意者请将本人应聘材料电子文档发至以下联络方式（请在应聘材料及邮件主题上注明
：姓名 ... 阅读全帖 |
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r*****m
发帖数: 3619 | 44 Results: 46
Select item 25597018
1.
Exome-wide Sequencing Shows Low Mutation Rates and Identifies Novel Mutated
Genes in Seminomas.
Cutcutache I, Suzuki Y, Tan IB, Ramgopal S, Zhang S, Ramnarayanan K, Gan A,
Lee HH, Tay ST, Ooi A, Ong CK, Bolthouse JT, Lane BR, Anema JG, Kahnoski RJ,
Tan P, Teh BT, Rozen SG.
Eur Urol. 2015 Jan 14. pii: S0302-2838(14)01396-7. doi: 10.1016/j.eururo.
2014.12.040. [Epub ahead of print]
PMID: 25597018 [PubMed - as supplied by publisher] Free Article
Related citations... 阅读全帖 |
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w***r
发帖数: 709 | 45 1. Nature. 2002 Jan 3;415(6867):45-53.
p53 mutant mice that display early ageing-associated phenotypes.
Tyner SD(1), Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu
X,
Soron G, Cooper B, Brayton C, Park SH, Thompson T, Karsenty G, Bradley A,
Donehower LA.
Author information:
(1)Cell and Molecular Biology Program, Baylor College of Medicine, Houston,
TX
77030, USA.
Comment in
Nature. 2002 Jan 3;415(6867):26-7.
The p53 tumour suppressor is activated by numerous stressors to ind... 阅读全帖 |
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m*******n
发帖数: 387 | 46 她在RW和PPP多混过
根据你给的linker：
Ma L, Chen Z, Erdjument-Bromage H, Tempst P, Pandolfi PP. Phosphorylation
and functional inactivation of TSC2 by Erk implications for tuberous
sclerosis and cancer pathogenesis. Cell 121(2):179-193, 4/2005. PMID:
15851026.
她先PPP然后在RW
不知道在PPP是学生还是博后 |
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j****x
发帖数: 1704 | 47 之前没留意过，近期突然发现，Clontech家的所有Lentiviral vector系统，包括其经
典的Tet-on/off/one lentivector，尽管宣传是最安全的第四代载体系统，但其中的
HIV-1 5'/3' LTR居然都是wildtype的而并非是被几乎所有其他商用载体所一致采用的
truncated/∆U3 LTR，作为self-inactivating (SIN) vector。这是件很奇怪的
事情，有哪位对Lentiviral vector比较熟悉的能讲讲吗？谢谢 |
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l********e
发帖数: 415 | 48 Rneasy Kits worked fine for us.
You do need to remove DNA as they can be incorporated into the sequencing
library.
However, DO NOT perform the standard on-column DNase treatment as it lead to
dramatic sample loss and often times not complete. I would just Dnase the
sample after purification and heat inactivate.
Please note that the regular Rneasy columns do not work well with RNA <
200bp. Therefore, if you want to look at small RNA, you might need a special
kit. |
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l********n
发帖数: 260 | 49 1. Zhang P, Wei Y, Wang L, Debeb BG, Yuan Y, Zhang J, Yuan J, Wang M,
Chen D, Sun Y, Woodward W, Liu Y, Dean DC, Liang H, Hu Y, Ang KK, Hung MC,
Chen J, Ma L. ATM-mediated stabilization of ZEB1 promotes DNA damage
response and radioresistance through CHK1. Nat Cell Biol. In Press.
2. Chen D, Sun Y, Yuan Y, Han Z, Zhang P, Zhang J, You MJ, Teruya-
Feldstein J, Wang M, Gupta S, Hung MC, Liang H, Ma L. miR-100 induces
epithelial-mesenchymal transition but suppresses tumorigenesis, migration
a... 阅读全帖 |
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w***a
发帖数: 210 | 50 1. Chitosan and mint mixture: A new preservative for meat and meat products
Food Chemistry
Volume 107, Issue 2, 15 March 2008, Pages 845–852
2. Tunisian Salvia officinalis L. and Schinus molle L. essential oils: Their
chemical compositions and their preservative effects against Salmonella
inoculated in minced beef meat
International Journal of Food Microbiology
Volume 125, Issue 3, 31 July 2008, Pages 242–251
3. Bacterial Spore Inhibition and Inactivation in Foods by Pressure,
Chemical Preservat... 阅读全帖 |
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