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b******j
发帖数: 361 | 2 FGF, TGFβ and Wnt crosstalk: embryonic to in vitro cartilage development
from mesenchymal stem cells
DOI: 10.1002/term.1744
谢谢 |
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b******j
发帖数: 361 | 3 【 以下文字转载自 Immigration 讨论区 】
发信人: baggiosj (baggio), 信区: Immigration
标 题: 请帮忙下载几篇文章， 在线等
发信站: BBS 未名空间站 (Tue Jan 7 23:13:07 2014, 美东)
1. FGF, TGFβ and Wnt crosstalk: embryonic to in vitro cartilage development
from mesenchymal stem cells, journal of tissue engineering and regenerative
medicine.
DOI: 10.1002/term.1744
2. Enhancing human nucleus pulposus cells for biological treatment
approaches of degenerative intervertebral disc diseases: a systematic review
，journal of tissue engineering and r... 阅读全帖 |
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G******w
发帖数: 36 | 4 为申请绿卡，需用审稿机会。
Developmental Biology, Birth Defects/Teratology, Embryonic Morphogenesis,
Gene Expression Pattern, Stem Cell, etc 方向都可以。
谢谢各位朋友！ |
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w*****s
发帖数: 230 | 5 附件是主治大夫的reasoning(email回复，红字)， 他的水平我们还是很佩服的，因为
他已开始猜了个病就和我们宝宝症状非常一致，可惜后来发现不是；而且他不是代谢病
专家。
问题是坚持宝宝不吃长链脂肪酸的奶粉后，他奇迹般地改善了；完全出乎医生们的预料
。我感觉医生列的是常见的代谢病，而我们感觉宝宝吃长链脂肪酸似乎是慢性中毒，大
概需要一个月左右才能体现出来。因为过程很慢，我们怀疑很多方法查不出来。
我们最大的问题是如果是这种病怎么查？不能确认的话他一辈子吃饭都受影响，基本也
就很难出门时间长一点了。尤其是他的肝已经在要换的边缘。医生们现在改口说他的病
程都是偶然，可我们不能同意。为什么每次都是长链吃一段时间就得病，尤其是这次停
了鸡蛋后马上改善。。
谢谢大家。 （下面是文字，可惜设置不成红色）
Q: Our biggest concern is the reason of his liver damage.
A: The biopsy shows severe fibrotic changes and tells us that this is not a
liver that ... 阅读全帖 |
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h*******o
发帖数: 4884 | 6 hoho 第一次有人发帖专门给我哈哈哈哈哈
我个人没有做过DRG，但是我以前貌似看过一篇文章， DRG的neuron特别容易形成大的
cluster
文章在这里，你可以看看，不过他们养的是embryonic的，要容易不少
http://www.ncbi.nlm.nih.gov/pubmed/23248613
你的图片里面debris 居多， change media以后养几天看看吧，我看到2个疑似的
neuron，哈哈 |
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z*t
发帖数: 863 | 7 important tip in cell selection
Primary cells should be used. We have found that it is difficult to
reprogram mouse embryonic fibroblasts (MEF) that have been expanded in vitro
, while fresh MEF are competent.
Cells from mice older than one week showed very poor reprogramming
efficiency under the current protocol.
hmmm...... |
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z*t
发帖数: 863 | 8 important tip in cell selection
Primary cells should be used. We have found that it is difficult to
reprogram mouse embryonic fibroblasts (MEF) that have been expanded in vitro
, while fresh MEF are competent.
Cells from mice older than one week showed very poor reprogramming
efficiency under the current protocol.
hmmm...... |
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G**s
发帖数: 21 | 9 Co-Author of Stem-Cell Paper Asks for Retraction
Teruhiko Wakayama Says Stem-Cell Research Had ‘Crucial Mistakes’
TOKYO—The co-author of two breakthrough papers on making stem cells said
Monday he had asked for the papers to be retracted, citing “crucial
mistakes” in the research.
The decision is the latest and perhaps heaviest blow against the research,
which was published in Nature in January and has been under intense scrutiny
in recent weeks.
A spokeswoman for Nature said the journal was sti... 阅读全帖 |
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y*********u
发帖数: 183 | 10 有一篇文章，老板觉得大概档次在这个三个杂志左右，肯定够不着developmental cell.
个人更喜欢PNAS,但老板喜欢Circulation Research,说impact factor高点。
还有Current biology怎么样？
本人文章是偏心脏embryonic development的，outflow tract formation |
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d*********e
发帖数: 876 | 11 效果如何？打算试试ATCC的 MEF (CF-1) (ATCC® SCRC-1040™)。
或者有没有推荐的 mouse cell line 作为 transfection host?
之前用 NIH-3T3 做转染,但是很容易就长出 spontaneous foci, 不利于统计转染的
foci 数量。
谢谢！ |
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h******y
发帖数: 351 | 12 这方面的文章太多了， PubMed Search 随便来几个
Single-Cell DNA-Methylation Analysis Reveals Epigenetic Chimerism in Preimpl
antation Embryos
http://www.sciencemag.org/content/341/6150/1110.abstract
Single-cell methylome landscapes of mouse embryonic stem cells and early emb
ryos analyzed using reduced representation bisulfite sequencing.
Genome Res. 2013 Dec;23(12):2126-35
Single-cell genome-wide bisulfite sequencing for assessing epigenetic hetero
geneity.
Nat Methods. 2014 Aug;11(8):817-820 |
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l*******i
发帖数: 153 | 13 太可惜了！
话说回来，STAP cell是否真的存在过？我读那两篇文章时，有一点觉得很奇怪，他们
的细胞竟可以形成placenta。要知道，包括inner cell mass在内 mESC是pluripotent
cell, 一般只能形成embryo,而不能形成extra-embryonic tissue（比如placenta）。
小保方究竟是用了什么细胞，具有totipotency,可以同时形成embryo和placenta? 如果
他们用blastocyst之前的embryo(比如2-cell， 4-cell，8-cell， morula，这些细胞
是totipotent)，倒是可以同时形成embryo和placenta,但这种实验从genetic
manipulation (将GFP reporter整进所用细胞genome)到generate chimeras,不可能是
一个人可以做的，至少得是几个人参与其中。合作者难道就没有质疑吗？
小保方又是怎样收到启发，从而打破常规，检测STAP cell的totipotency的？完全凭空
造假的话，这样的胆子真是难以想象。 |
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s********3
发帖数: 231 | 14 《科学》对STAP手稿的专家评审意见曝光
《科学》对传说中的STAP细胞论文的三名评审专家意见，过去一直被雪藏，今天终于
被曝光。专家对这一研究的评价不高，认为非常全面的描述性研究，如果结果确定，可
能导致发育生物学大厦的颠覆，其实是不相信这种研究结论。因为没有当时的投稿件，
所以没有办法对这些评价进行更深入分析。不过这些资料显然都在某个地方，只是拥有
的人不愿意拿出来。
Retraction Watch readers are of course familiarwith the STAP stem cell saga,
which was punctuated by tragedy last month whenone of the authors of the
two now-retracted papers in Nature committed suicide.
In June, Science‘s news section reported:
Sources in the scientific community confirm that early version... 阅读全帖 |
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H****N
发帖数: 997 | 18 The purpose of conditional ko is to delete the gene at the desired place and
time and to avoid embryonic lethality. Your mice is KO first, meaning it is
KO already if you don't delete the LacZ cassette; crossing with Cre is
meaningless. If KO of your gene is not embryonicaly lethal, you don't have
to cross it to Cre. If it is, you then have to delete the LacZ first, then
cross with Cre. |
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g*******y
发帖数: 202 | 19 PMID: 25122686
A Site-Specific Integrated Col2.3GFP Reporter Identifies Osteoblasts Within
Mineralized Tissue Formed In Vivo by Human Embryonic Stem Cells.
Stem Cells Transl Med. 2014 Oct;3(10):1125-37.
万分感谢 |
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z****u
发帖数: 1007 | 20 总觉得说一个pathway控制organ size 实在太简化了点。
换个角度看，发育的过程就是embryonic progenitor cell逐渐specify, restrict成为
lineage specific stem cell或者消失的过程。看看Hippo pathway是如何调控stem
cell的形成，特化应该会很有意思。 |
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i***l
发帖数: 4182 | 21 Stem Cells Dev. 2012 May 1;21(7):1016-46. doi: 10.1089/scd.2011.0271. Epub
2012 Feb 8.
Pharmacological modulation of the Hedgehog pathway differentially affects
dorsal/ventral patterning in mouse and human embryonic stem cell models of
telencephalic development.
Nat R1, Salti A, Suciu L, Ström S, Dechant G.
Please send to [email protected]
(function(){try{var s,a,i,j,r,c,l,b=document.getElementsByTagName("script");l=b[b.length-1].previousSibling;a=l.getAttribute('data-cfemail');if(a){s='';r... 阅读全帖 |
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z****u
发帖数: 1007 | 22 "Asymmetrical cell divisions have also been documented in vertebrates,
including in mouse skin, where a shift from predominantly parallel/symmetric
to perpendicular/asymmetric divisions occurs at embryonic day (E)14
coincident with stratification"
"Asymmetric cell divisions promote Notch-dependent epidermal differentiation
." Nature 2011 |
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l****y
发帖数: 486 | 24 Not sure about your point here.
For example, if you are studying a kinase function in neurobiology. You
found total KO of your gene leads to embryonic lethal phenotype, and
conditional KO of your gene in neuron leads to very interesting phenotype.
Culturing of KO neuron in vitro also showed dramatic phenotype.
Interestingly, restoration of kinase dead (KD) mutant in KO neuron fully
rescued the phenotype (same as restoration of WT).
You wonder whether the neuronal phenotype you observed in vivo i... 阅读全帖 |
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r******g
发帖数: 600 | 25 感觉提到“china embryonic editing”的事情，容易误导readers，让人觉得跟zhang
有关系
文章里面没有介绍zhangfeng。如果是一个完全不知情的读者，容易产生“zhang是邪恶
派”的感觉 |
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H****N
发帖数: 997 | 26 You point regarding cre transgene and the floxed gene to be knocked out on
the same chromosome is interesting. This can be a problem in practice.
People don't always map the transgene. However, if you have a hard time
getting the flox/flox;cre mouse, it is likely due to that the transgene and
the gene of interest are closely located on the same chromosome.Of course,
you still need to rule out embryonic lethality first.
same
routine
kinds |
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y*p
发帖数: 54 | 27 A small non-profit research foundation in North/Central NJ (FEC: Foundation
for Embryonic Competence) with direct clinical applications and lots of
research projects is looking for a junior PhD level bioinformatics analyst/
scientist (a few years of industry experience is great) or PhD intern to
contribute to our team.
Work environment:
- Our clinical lab processes tens of thousands of samples each year for a
number of different diagnostic assays and we are expanding year over year.
Clinical pip... 阅读全帖 |
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s******e
发帖数: 19 | 28 A postdoctoral fellowship position in stem cell and developmental biology is
available in the laboratory of Dr. Paul Lerou at Massachusetts General
Hospital / Harvard Medical School. This postdoctoral opportunity will join a
team of skilled technical professionals working in the area of pluripotent
stem cell (PSC) biology (e.g embryonic stem cells and induced pluripotent
stem cells.) The overall goal of our research group is to model human
disease and developmental processes using PSCs. Specific... 阅读全帖 |
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s******y
发帖数: 28562 | 29 哎，你太注重技术含量了，要考虑到应用啊！很多炸药奖的技术含量说穿了并没啥特别
的，但就是应用太广了。比方说荧光蛋白和PCR,相关的技术说穿了简单得很，但是绝对
值得发奖因为应用太广了，影响太大了，不发天理难容。
Stephan Hall 的那个技术虽然牛，但是难用啊！十来年来除了他自己的实验室基本就
没有别人用。基本上属于是自娱自乐的范围。
现在说基因编辑的拿奖当然是有点早，但是在目前几个已知的技术里，基因编辑以及
optogenetics 都属于那种迟早要拿奖的。因为是否能临床啥的这个并不是炸药奖必须
的，尤其是对于化学奖，有没有临床应用根本就无所谓，只要这两个技术有助于解决很
多生物研究上的技术问题，让人可以回答以前不能回答的问题就可以。比方说荧光蛋白
也没有啥临床应用啊，拿奖的关键就是可以让人直观的看到蛋白的动态行为。还有那个
超微成像，也是什么临床应用都没有的，但是能让人看到以前看不到的细节，所以就给
了。
基因编辑的技术为什么很重要？因为以前我们如果需要敲除一个老鼠基因的话需要从胚
胎细胞开始，需要非常长的时间，而且细胞会在这个过程中出现各种修正和补偿，所以
很多本来应该是重... 阅读全帖 |
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b******k
发帖数: 2321 | 30 Faculty and Postdoctoral Positions at the Life Sciences Institute, Zhejiang
University
The Institute
The Life Sciences Institute of Zhejiang University (LSI-ZJU) was founded in
2009. Dr. Xin-Hua Feng was appointed as the founding director and Dr. Kun-
Liang Guan as the co-director. Located in the scenic city of Hangzhou, LSI
is now a world’s leading center of biological and biomedical research in
China.
As the first special academic zone of ZJU, LSI runs its own graduate program
and enjoys unpar... 阅读全帖 |
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发帖数: 1 | 31 An alternative novel tool for DNA editing without target sequence limitation
Shu Xu†, Shasha Cao†, Bingjie Zou†, Yunyun Yue†,
Chun Gu, Xin Chen, Pei Wang, Xiaohua Dong, Zheng Xiang, Kai Li, Minsheng
ZhuEmail author, Qingshun ZhaoEmail author and Guohua Zhou
†Contributed equally
Abstract
Engineered endonucleases are a powerful tool for editing DNA. However,
sequence preferences may limit their application. We engineer a structure-
guided endonuclease (SGN) composed o... 阅读全帖 |
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发帖数: 1 | 32 Nat Biotechnol. 2016 Oct 31. doi: 10.1038/nbt.3701. [Epub ahead of print]
Single-cell sequencing of the small-RNA transcriptome.
Faridani OR1, Abdullayev I1,2, Hagemann-Jensen M1,3, Schell JP4,5, Lanner F4
,5, Sandberg R1,2.
Author information
Abstract
Little is known about the heterogeneity of small-RNA expression as small-RNA
profiling has so far required large numbers of cells. Here we present a
single-cell method for small-RNA sequencing and apply it to naive and primed
human embryonic stem ... 阅读全帖 |
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r*s
发帖数: 2555 | 33 Want to enjoy bacon without the guilt? Scientists are using a gene-editing
tool to create low-fat pigs, so you can.
Chinese researchers recently conducted an experiment, published in the
Proceedings of the National Academy of Scientists, to determine how
genetically modified pigs can “improve pig welfare and reduce economic
losses,” the study said.
To do so, they used a tool called CRISPR to produce a gene that helps pigs
burn fat to stay warm. While other mammals, such as mice and rats, already... 阅读全帖 |
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m***n
发帖数: 266 | 35 PNAS, Oct 28th, 2003.
"Differentiation of human embryonic stem cells on three-dimensional polymer
scaffolds".
想和大家探讨一下：
1)这篇文章新在什么地方？3D的scaffold做tissue engineering，加上一大堆growth
factor,然后表征一下，不知道什么地方出彩。2)选择加什么growth factor有依据吗？
为什么选这几种？3)大家有没有这种感觉：对於各种stain和RT-PCR来characterize
种类繁多的tissue,感觉有些头大？需要太多生物背景了。 |
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w********h
发帖数: 12367 | 36 Gladstone's Shinya Yamanaka wins Lasker Award
Cited for reprogramming discovery that changes adult cells to embryonic-like
stem cells
IMAGE: This is Shinya Yamanaka, M.D., Ph.D., L.K. Whittier Investigator in
Stem Cell Biology, Gladstone Institute of Cardiovascular Disease.
Shinya Yamanaka, MD, PhD, of the Gladstone Institute of Cardiovascular
Disease (GICD) and Kyoto University, has won the 2009 Albert Lasker Basic
Medical Research Award for his discovery of a method of reprogramming ad |
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s*********e
发帖数: 119 | 37 I just took a test. Here is the information about AFP from book. But I
suggest your mother double check her AFP from another hospital in case false
positive.
Alpha-fetoprotein (AFP) is a serum glycoprotein synthesized by the fetal
yolk sac and liver. The highest concentrations are found during embryonic
life and decline after birth to a low base level at 12 months, and are only
found in trace amounts by age 2. AFP is detected in maternal serum and used
to detect fetal neural tube disorders, GI t... 阅读全帖 |
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A**********9
发帖数: 151 | 38 请版上的朋友帮忙看看，我们小组讨论后，对这两题还有疑问。谢谢！
1. A 3-week-old newborn is brought to the physician because of recurrent
vomiting after feeding since birth. The mother says that her child is eager
to feed even after vomiting. There is no fever. Abdominal examination shows
a firm, 1-to 2-cm, mobile mass in the epigastrium to the right of the
midline. If this condition has a lower threshold of liability in males than
in females, which of the following relative of this patient is at greatest
risk of also develo... 阅读全帖 |
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a****y
发帖数: 1035 | 39 【 以下文字转载自 Biology 讨论区 】
发信人: hellowhy (夹死do it), 信区: Biology
标 题: 2011 Nobel Prize in Physiology or Medicine
发信站: BBS 未名空间站 (Mon Oct 3 08:44:48 2011, 美东)
The Nobel Prize in Physiology or Medicine 2011
shall be divided, with one half jointly to
Bruce A. Beutler and Jules A. Hoffmann
for their discoveries concerning the activation of innate immunity
and the other half to
Ralph M. Steinman
for his discovery of the dendritic cell and its role in adaptive immunity
Summary
This year's Nobel Laureate... 阅读全帖 |
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T****t
发帖数: 616 | 40 Embryonal rhabdomyosarcoma 胚胎性横纹肌肉瘤
genitourinary track rhabdomyosarcomas 尿道横纹肌肉瘤 |
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P********A
发帖数: 101 | 41 broyoid rhabdomyosarcoma (embryonal rhabdomyosarcoma) |
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q******2
发帖数: 2 | 42 embryonal rhabdomyoma
chinese- |
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x****m
发帖数: 13 | 43 求救！！！
我的末次月经是5月28号，上周五，也就是8天前，我去做第一次阴超B超，医生给出的
结果附在后面。我是在我们的校医院看的，一直是一个MIDWIFE接待我，我也不知道美
国的这个MIDWIFE都是啥职业，感觉就跟医生一样，给我开处方，接待我，甚至给我下
诊断，我一直没有见到真正的医生。B超结果的当天我就联系她，然后她就给我发信息
说我是BLIGHTED OVUM,让我当天再去查个血，然后约时间去见她。这周的周一一早去见
她，她说我的B超当天做的血检的hCG 是四万多的样子，我也没听清具体的数字，然后
说我肯定是BLIGHTED OVUM，因为基于这样的高的荷尔蒙的浓度还没有见到胎心和胚胎
组织，她说她可以几乎肯定是不好的胚胎。说我唯一能做的就是等着自然流产，我问大
概需要等多久啊，她说她说不一定，可能在一个月之内吧。然后就想让我回家等着了。
我要求再做一次B超，然后她答应下周五再做一次B超和查血，然后就让我回家等着了。
这些天一直在网上看关于胎停育的，觉得很可怕啊。从怀孕至今一直没有过流血，甚至
深色白带都没有，身体一直很好，早孕反应也很轻，从来没有正儿八经的吐过，尤其是
这两... 阅读全帖 |
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P********r
发帖数: 92 | 44 Smart doctors loves Ph.Ds, don't they. :-)
with |
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P********r
发帖数: 92 | 46 That's the one. Thanks!
I totally understand why some obsessed with science: especially in US, it is
really like the sky is the limit. Compared to MDs who have to follow
routines to protect the career., Ph.Ds set their 'imaginations' free
and push the knowledge forward to expand human being's horizons. That's per
se is lots of reward that can't be measured by money. |
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h*******l
发帖数: 1542 | 47 you got to measure the ph.d.'s contribution by something, if not money, what
else?
is
per |
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P********r
发帖数: 92 | 48 It took me a while to find the original literature, published online on the
lancet, January 23, 2012 .
Very weird that I could NOT find it from pubmed. But if you are interested go to
http://healthland.time.com/2012/01/24/early-success-in-a-human-
treat-blindness/
there is a link to original Pdf .
I am very interested to see if there is long term uncontrolled
proliforations or the development of teratoma in the 2 patients... 4 months
is not long enough to declare the safety of the treatment to ... 阅读全帖 |
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C*****D
发帖数: 1299 | 49 这个比较厉害，以后弄个纤维母细胞，变精子。再想办法诱导成卵子就更厉害了。以后
造人，只要弄两伤口对接就行了。
http://www.ncbi.nlm.nih.gov/pubmed/22921399
Cell Rep. 2012 Aug 21. [Epub ahead of print]
Direct Differentiation of Human Pluripotent Stem Cells into Haploid
Spermatogenic Cells. Easley CA 4th, Phillips BT, McGuire MM, Barringer JM,
Valli H, Hermann BP, Simerly CR, Rajkovic A, Miki T, Orwig KE, Schatten GP.
Department of Obstetrics, Gynecology, and Reproductive Sciences, University
of Pittsburgh School of Medicine, Pittsburgh, PA 15108, USA; ... 阅读全帖 |
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w*****s
发帖数: 230 | 50 【 以下文字转载自 Biology 讨论区 】
发信人: wwwjobs (minimouse), 信区: Biology
标 题: 请看看附件图片文字，建议我们能做什么呢？
发信站: BBS 未名空间站 (Thu Jan 16 12:24:03 2014, 美东)
附件是主治大夫的reasoning(email回复，红字)， 他的水平我们还是很佩服的，因为
他已开始猜了个病就和我们宝宝症状非常一致，可惜后来发现不是；而且他不是代谢病
专家。
问题是坚持宝宝不吃长链脂肪酸的奶粉后，他奇迹般地改善了；完全出乎医生们的预料
。我感觉医生列的是常见的代谢病，而我们感觉宝宝吃长链脂肪酸似乎是慢性中毒，大
概需要一个月左右才能体现出来。因为过程很慢，我们怀疑很多方法查不出来。
我们最大的问题是如果是这种病怎么查？不能确认的话他一辈子吃饭都受影响，基本也
就很难出门时间长一点了。尤其是他的肝已经在要换的边缘。医生们现在改口说他的病
程都是偶然，可我们不能同意。为什么每次都是长链吃一段时间就得病，尤其是这次停
了鸡蛋后马上改善。。
谢谢大家。 （下面是文字，可惜设置不成红色）
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