M*****e
发帖数: 279 | 1 饶毅: 你可以有自己的观点，但不能不懂的还充内行。
1.对肖传国的工作的小看。他虽然广义上是研究neuroscience，但不代表他就知道肖的
探索(nerve rerouting)的重要意义。虽然肖有夸大他的手术的疗效（对有些spinal
bifilda孩子的排尿控制还是有效的。），手术在概念上开创性的。需要时间来完善这个
手术。至少一个美国JHU 的pediatric neurosurgeon知道他的手术；并认为是创新；如
果成功，will be amazing.
饶毅虽然是学医的，毕业于江西医学院，但是并没有行医。也不一定对因为脊髓损伤（
各种原因）导致排尿障碍有研究和深入了解，但是他如同方舟子一样说肖的手术一文不
值；肖是弱智，是充内行了。
2.过分贬低水通道aquaporin获得诺奖。
下面是我曾经附和一个帖子的观点。
http://www.mitbbs.com/article_t1/Biology/31504219_0_1.html
Totally agree what you (chugol) said.
Suprisingly, Yao Yi did not appre... 阅读全帖 |
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i***l
发帖数: 4182 | 2 Odd-Skipped Related Genes Regulate Differentiation of Embryonic Limb
Mesenchyme and Bone Marrow Mesenchymal Stromal Cells
Sigmar Stricker,1,2 Susanne Mathia,1 Julia Haupt,1,3 Petra Seemann,1,3 Julia
Meier,1 and Stefan Mundlos1,2,3, Stem Cells and Development
please send to i******[email protected]
Greatly appreciate! |
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l**********1
发帖数: 5204 | 3 LZ were late from Cell already published:
please go to
//www.ncbi.nlm.nih.gov/pubmed/21414480
Cell. 2011 Mar 18;144(6):874-
It is clear that the Xenopus embryonic cell cycle can operate in
the absence of transcription. Therefore, we have regarded the
cell-cycle oscillator as only a protein circuit—proteins regulate
other proteins, but not gene expression. Nonetheless, many cellcycle
regulators in many cell types undergo periodic transcription
(Spellman et al., 1998). Indeed, in budding yeast, tr... 阅读全帖 |
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l**********1
发帖数: 5204 | 4 LZ were late from Cell already published:
please go to
//www.ncbi.nlm.nih.gov/pubmed/21414480
Cell. 2011 Mar 18;144(6):874-
It is clear that the Xenopus embryonic cell cycle can operate in
the absence of transcription. Therefore, we have regarded the
cell-cycle oscillator as only a protein circuit—proteins regulate
other proteins, but not gene expression. Nonetheless, many cellcycle
regulators in many cell types undergo periodic transcription
(Spellman et al., 1998). Indeed, in budding yeast, tr... 阅读全帖 |
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r**a
发帖数: 121 | 5 需要这个文献，但是我们学校没有办法下载！哪位达人，帮忙发送到：
l***********[email protected]
十分感谢！！
Apterous is a Drosophila LIM domain gene required for the development of a
subset of embryonic muscles.
Bourgouin C, Lundgren SE, Thomas JB. |
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a*****x
发帖数: 901 | 6 今天随意瞥到一篇不错的paper:
Cell Stem Cell. 2012 Mar 15. [Epub ahead of print]
Human Embryonic Stem Cell-Derived GABA Neurons Correct Locomotion Deficits
in Quinolinic Acid-Lesioned Mice.
等有时间把心得写下来征求牛牛们的看法 |
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a*****x
发帖数: 901 | 7 They are definitely not neurons. It's a transformed embryonic cell line,
with a few properties similar as immature neurons, but they don't have any
neuronal functions (such as action potentials). |
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l**********1
发帖数: 5204 | 8 alternatively you can try Oligotex-dt30 mRNA purification kit for total RNA
sample:
Manual book:
//catalog.takara-bio.co.jp/PDFFiles/NTrap%20mRNA_j.pdf
Vender link:
//www.mn-net.com/Products/DNAandRNApurification/RNA/NucleoTrapmRNA/tabid/1328/language/en-
US/Default.aspx
Reference:
please go to
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/18678863
T Yabu - 2008 JBC
31 Oct 2008 – In zebrafish embryonic cells, the endogenous SMase enzyme was
.... using the Oligotex-dt30 mRNA purification kit (Tak... 阅读全帖 |
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v**t
发帖数: 74 | 9 求文献全文，先谢了!
g**********[email protected]
Cytotherapy, 2008, Vol. 10, No. 4 : Pages 376-389
Highly enriched cardiomyocytes from human embryonic stem cells
XQ Xu, R Zweigerdt, XQ Xu, R Zweigerdt, SY Soo, ZX Ngoh, SC Tham, ST Wang, R
Graichen, B Davidson, A Colman, and W Sun |
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h*****G
发帖数: 113 | 10 human embryonic stem cell， 用puromycin和G418 select double positive 的细胞。
一般大家是puromycin和G418一起加，还是先加puromycin，过几天后再加G418? 谢谢。 |
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s******s
发帖数: 13035 | 11 field
mouse epigenetics, stem cell
fly embryonic development, pattern formation, genetics
protein glycosylation |
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r*******y
发帖数: 48 | 12 if the deletion of the gene of your interest is embryonically lethal, and
there is no significant difference in the expression pattern of this gene in
various tissues/organs, then you need to know:
1. what was the major cause of embrynic death once this gene was deleted
globally? most of genes, although ubiquitously expressed, have more
important roles in the development/function of some organ(s) than others.
depending on the developental stage when the null embryos died, most
possible cause of ... 阅读全帖 |
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G********e
发帖数: 528 | 13 I'm quite convinced by your logic.
I only know the embryos do not develop beyond E5, which might be considered
quite early. I could not get any hint form embryonic study for tissue
specificity.
Anyway, thank you very much! Really help me think about it. |
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l**********1
发帖数: 5204 | 14 sunnyday
papers 比这个香港大学的 AP Japanese 的零头多吗?
>Hiiii obtained his medical degree from Miyazaki Medical College (presently
University of Miyazaki), Japan in 2002 and then completed his PhD study at
Hiroshima University. During graduate and postgraduate training periods, he
spent 7.5 years in Europe, working at Imperial College London, University of
Tuebingen and University of Utrecht as an infectious disease modeler. His
research interests span the areas of statistical epidemiology of infectious
di... 阅读全帖 |
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G********e
发帖数: 528 | 15 Nature 457, 854-858 (12 February 2009) | doi:10.1038/nature07730; Received
16 October 2008; Accepted 18 December 2008
ChIP-seq accurately predicts tissue-specific activity of enhancers
Axel Visel1,4, Matthew J. Blow1,2,4, Zirong Li3, Tao Zhang2, Jennifer A.
Akiyama1, Amy Holt1, Ingrid Plajzer-Frick1, Malak Shoukry1, Crystal Wright2,
Feng Chen2, Veena Afzal1, Bing Ren3, Edward M. Rubin1,2 & Len A.
Pennacchio1,2
Genomics Division, MS 84-171, Lawrence Berkeley National Laboratory,
Berkeley, Cal... 阅读全帖 |
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l**********1
发帖数: 5204 | 16 LZ 的细胞培养过程中 有stress 的话
it might happened. (NB: here stress likes oxgen concentration value)
please refer
Fernandes TG et al. (2010)
Different stages of pluripotency determine distinct patterns of
proliferation, metabolism, and lineage commitment of embryonic stem cells
under hypoxia.
Stem Cell Res. 5: 76-89.
citation:
>beta-tubulin (Tuj1) revealed a slightly higher percentage of
neuronal cells at low oxygen tension that
link:
//www.ncbi.nlm.nih.gov/pubmed/20537975 |
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l**********1
发帖数: 5204 | 17 Here you go
Deramaudt TB et al. (2011)
FAK phosphorylation at Tyr-925 regulates cross-talk between focal adhesion
turnover and cell protrusion.
Mol Biol Cell. 22: 964-75.
Abstract
Cell migration is a highly complex process that requires the coordinated
formation of membrane protrusion and focal adhesions (FAs). Focal adhesion
kinase (FAK), a major signaling component of FAs, is involved in the
disassembly process of FAs through phosphorylation and dephosphorylation of
its tyrosine residues, but ... 阅读全帖 |
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l****j
发帖数: 70 | 18 MEF cell，第一代长的很好，
第二代就长的乱七八糟了，形态都不太规则，有大有小，还有多核的，有的都不像
fibroblast了
我完全照着protocol做的，medium里都加mercaptoethanol了
Current Protocols in Molecular Biology (2005) 28.1.1-28.1.8
UNIT 28.1
Preparation, Culture, and Immortalization of Mouse Embryonic Fibroblasts
有人有类似情况吗？
MEF cell怎么能长得好？ |
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j****1
发帖数: 81 | 19 Isolation of embryonic stem (ES) cells in media supplemented with
recombinant leukemia inhibitory factor (LIF)；
Dev Biol. 1990 Oct;141(2):344-52. |
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a*****x
发帖数: 901 | 20 这篇文章开头就有的：
“We have shown previously that embryonic stem cells (ESCs) and induced
pluripotent stem cells (iPSCs) from male (XY) mice are induced into epiblast
-like cells (EpiLCs), which are in turn induced into primordial germ cell–
like cells (PGCLCs) with proper function for spermatogenesis and offspring
production”
所以精子用的是XY
oocyte |
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l**********1
发帖数: 5204 | 21 En
one aspect:
To keep stability and safety of Somatic cell Reprogramming to iPS cell
pls refer
Hamada M. et al. (2012)
Reprogramming to Pluripotency Can Conceal Somatic Cell Chromosomal
Instability.
PLoS Genet. 8: e1002913.
Abstract
ignored
These findings suggest that aneuploid cells can be selected for or against
during reprogramming depending on the W-CIN gene defect and present the
novel concept that somatic cell W-CIN can be concealed in the pluripotent
state. Thus, karyotypic analysis of s... 阅读全帖 |
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l**********1
发帖数: 5204 | 22 ZZ
幹細胞是一種能自我增生並且
具有分化成ㄧ到數種細胞潛能的細胞。依據它分化
潛能可分為單效性（unipotent，分化為一
種細胞）、複效性（pluripotent，分化成胚胎期
同源細胞）和全效性（totipotent，幾乎可分
化成身體中任何一種細胞）
----
我們稱這些內層細胞為〝複效性的〞（pluripotent）──也
就是他們可以形成多種類型的細胞但是並非
胎兒發育所需要的所有細胞類型。由於其效能並非
完全的，他們不是全效性的也非胚胎本身，
事實上，如果將一個內層細胞放在子宮內，
它並無法形成一個胎兒。
-----
三、細胞療法的細胞來源
幹細胞依其來源可分為胚胎幹細胞（embryonic stem cells）
及成體幹細胞兩種。胚胎幹細胞來自於囊胚的內層
細胞團，是一種複效性幹細胞（pluripotent stem cells）
而成體幹細胞則是由成體各種器官或組織裡所分離
出來的幹細胞。在不同的成體幹細胞中，造血幹
細胞（hematopoietic stem cells）以及
間葉幹細胞（mesenchymal stem cells）一直是研
究的熱門題目，尤其... 阅读全帖 |
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O******e
发帖数: 4845 | 23 Press Release
2012-10-08
The Nobel Assembly at Karolinska Institutet has today decided to award
The Nobel Prize in Physiology or Medicine 2012
jointly to
John B. Gurdon and Shinya Yamanaka
for the discovery that mature cells can be reprogrammed
to become pluripotent
Summary
The Nobel Prize recognizes two scientists who discovered that mature, specia
lised cells can be reprogrammed to become immature cells capable of developi
ng into all tissues of the body. Their findings have revolutionised our... 阅读全帖 |
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b*****n
发帖数: 1841 | 24 Losing out on a Nobel Prize
Updated: Mon, Oct 08 2012. 07 07 PM IST
New Delhi: This year’s Nobel Prize for Medicine underlines the slender
margin between winning and losing.
Monday’s announcement of the prize to John Gurdon at Cambridge University
and Shinya Yamanaka of Kyoto University for discovering and showing in
different ways that even matured cells—or adult stem cells as they are
called—that are destined to be skin or nerve or intestinal cells, could be
programmed into an immature state.
... 阅读全帖 |
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l**********1
发帖数: 5204 | 25 DEVELOPMENT - SCI影响因子(2011年)
Semimonthly 半月刊 月刊
通讯方式
COMPANY OF BIOLOGISTS LTD, BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY
RD, CAMBRIDGE, ENGLAND, CAMBS, CB4 4DL
研究方向: 胚胎发育机理 组织发育 器官发育 投稿周期: 约3个月 IF(2007): 7.293
IF(2008): 6.812 IF(2009): 7.194 命中率: 约20%
经典的专业杂志，她的scope主要是胚胎发育机理”insights into the mechanisms of
embryonic development“，这一点在以杂志的原名Journal of Embryology and
Experimental Morphology更能体现出来。许多大牛曾在JEEM上发表了足以载入胚胎学
发展史的经典论文。1987年之后，随着发育学科的发展，科学家有了有效的手段可以研
究组织和器官水平上的发育问题，杂志侧重的研究方向也... 阅读全帖 |
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s******a
发帖数: 472 | 26 还真有可能啊，标题还有异曲同工之妙
Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult
Fibroblast Cultures by Defined Factors
. |
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C********4
发帖数: 308 | 27 分化成神经不是最简单的吗
Bibel M, Richter J, Lacroix E, Barde Y-A (2007) Generation of a defined and
uniform population of CNS progenitors and neurons from mouse embryonic stem
cells. Nat Protoc 2: 1034–1043 |
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k******2
发帖数: 81 | 28 Stem Cells. 2012 Aug;30(8):1685-95. doi: 10.1002/stem.1142.
HIF-2α suppresses p53 to enhance the stemness and regenerative potential of
human embryonic stem cells. |
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T*********l
发帖数: 298 | 29 Methods in Molecular Biology
2012, Volume 926, 317-329,
DOI: 10.1007/978-1-62703-002-1_20
Title:
Application of embryonic and adult zebrafish for nanotoxicity assessment.
email: g*******[email protected]
有包子！谢谢！ |
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c********r
发帖数: 189 | 30 我想做个screen y2h， 不知道自己做好还是找公司好些，打算用小鼠Embryonic brain
library screen, 大家说说screen的话要多少钱啊？ 多谢！ |
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A*******e
发帖数: 13 | 31 ：转一个，听说还有些比较激进的言论没有收在文字稿里。
中科院神经科学研究所2012 年年会蒲慕明所长的讲话
谈灰色地带的科研诚信
2012 年12 月28 日
（根据录音整理）
一、前言
我今天想讲的主题是过去主题的延续。几年前我曾经谈过诚信的问题。科学
研究的诚信问题现在越来越重要。媒体上常有关于科学不端行为的报导，但事实
上明目张胆的科学不端行为（scientific misconducts），所谓伪造、篡改和剽窃
（Fabrication，Falsification，Plagiarism，简称FFP），是极少见的。现在最严重
的问题不是明显的FFP，而是灰色地带的诚信问题，就是那些不容易被发现、标
准不容易确定的问题。我们每一个从事科学研究的人都会遇到这些问题，都应该
认真考虑，当遇到这些问题时，怎么做才符合诚信的要求。
二、数据分析和表述时的灰色地带行为
今天我首先想讲的就是数据分析和表述时的不当行为。首先，较严重的就是
选择数据。只选择自己喜欢的结果，不喜欢的就不要。忽略不计对自己的结论有
威胁性的数据。第二，在发表文章或口头表述结果时不当地夸大表述。前者比较
容易判断是非，... 阅读全帖 |
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x********e
发帖数: 2275 | 32 请发到：h******[email protected]
先谢过了！
Curr Med Chem. 2012 Dec 1;19(35):6065-71.
Characterisation of a Neural Teratogenicity Assay Based on Human ESCs
Differentiation Following Exposure to Valproic Acid.
Colleoni S, Galli C, Gaspar JA, Meganathan K, Jagtap S, Hescheler J,
Sachinidis A, Lazzari G.
SourceAvantea, Via Porcellasco 7/f, 26100 Cremona, Italy. silviacolleoni@
avantea.it.
Abstract
The development of in vitro testing strategies for chemical and drug
screening is a priority need in order to protect... 阅读全帖 |
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S******1
发帖数: 6 | 33 PhD student position available
Department of Molecular Cell Biology, Leiden University Medical Center, The
Netherlands
Overview of the project and role
We are looking to recruit a PhD student to investigate the role of TGF-&#
61538; family members in liver fibrosis and cancer. A combination of
molecular biology, biochemistry, cell biology and animal model based
techniques will be used. The research project will be performed at the
Department of Molecular Cell Biology, Leiden University Medical ... 阅读全帖 |
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Q**********r
发帖数: 214 | 34 Small. 2013 Jan 30. doi: 10.1002/smll.201202340. [Epub ahead of print]
Nanotopographical Cues Augment Mesenchymal Differentiation of Human
Embryonic Stem Cells.
Kingham E, White K, Gadegaard N, Dalby MJ, Oreffo RO.
请发至 a*************[email protected]
谢谢！ |
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D****g
发帖数: 275 | 35 大家都说primary fibrablast不好转染。我们实验室有一种mouse embryonic
fibroblast, 虽说是primary cell, 但是已经建立好多年了，估计是自然transformed
了，前两天试了一下，转染效率能有90％以上。一直纳闷，为什么primary cell不容易
转染哪？是因为生长慢吗？ |
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r***e
发帖数: 2539 | 36 不是说myc没有具体的target吗？是整个转录系统放大。
看最近的两篇back to back文章在Cell。
Lin, C. Y. et al. Transcriptional amplification in tumor cells with elev
ated c-Myc. Cell 151, 56–67 (2012)
Nie, Z. et al. c-Myc is a universal amplifier of expressed genes in lymp
hocytes and embryonic stem cells. Cell 151, 68–79 (2012)
http://www.nature.com/nrg/journal/v13/n11/full/nrg3364.html |
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p*****c
发帖数: 20445 | 37 A-D E-K L-P R-Z
Peter Baumann, Ph.D.
HHMI Early Career Scientist
Stowers Institute for Medical Research
Peter Baumann’s research focuses on beginnings and endings: beginnings in
the form of reproduction in unisexual lizards and endings in the form of the
telomeres that protect chromosome tips.
Each time chromosomes are copied, telomeres—specialized stretches of DNA
that extend from the ends of each chromosome—become progressively shorter.
With each cell division, they ero... 阅读全帖 |
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p*****c
发帖数: 20445 | 38 Christopher D. Lima, Ph.D.
Memorial Sloan-Kettering Cancer Center
Christopher Lima doesn’t pick easy problems. A structural biologist, he is
investigating how cells attach small proteins—like ubiquitin and the
related molecule SUMO—to other proteins to modify their function or fate.
Alongside those studies, he is exploring the molecular mechanisms that
underlie RNA processing and degradation—modifications to an RNA copy of a
gene that influence its stability and ability to be used as a template ... 阅读全帖 |
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l******g
发帖数: 1145 | 39 是的，30％左右的penetrance其实已经不错了。
比较意外，是其中一个基因的完全ko完全没有表型，所以没有预期double het会有表型
。现在比较担心完全ko后，就embryonic lethal了，只能分析下genetic interaction。 |
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i****g
发帖数: 3896 | 40 这篇是关于胚胎干细胞定向分化的。
http://www.cell.com/cell-stem-cell/abstract/S1934-5909(13)00272
Directed Differentiation of Human Embryonic Stem Cells into Thymic
Epithelial Progenitor-like Cells Reconstitutes the Thymic Microenvironment
In Vivo
6月研究iPS机制的Cell封面文章，7月4个小分子化合物诱导iPS的Science文章，刚到8月
又有Cell Stem Cell，今年集中爆发了啊. |
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T****u
发帖数: 424 | 41 NIBS:
王晓东: Several key molecules in necroptosis(RIP3,MLKL etc)
邵峰: Mechanism of pathogen-host interaction
(key:NLRC4 recognize flagellin)
李文辉 NTCP as HBV receptor <======milestone
张宏: Delineate autophagy in C.elegan
朱冰：Histone code
厦大：
韩家怀: one key molecule in necroptosis(RIP3)
上海生化所:
徐国良mechanism of DNA demethylation;
李劲松 haploid ES
动物所
周琦 transgenic mice using androgenetic haploid embryonic stem
北京大学
邓鸿奎 CiPS <======milestone
CiPS is more important... 阅读全帖 |
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n***w
发帖数: 2405 | 42 这两年视觉研究的地震不少，
rd8这个新的突变于12年报导出来，对搞视觉研究的震撼很大，所有实验室回去筛选有
没有这个mutation，因为这个mutation和其他的rd突变不太一样的一点是除了直接的导
致视网膜退变，还有一些其他的表型，这个突变在c57bl/6n的老鼠里，c57bl/6j的没有
事儿。以至于之前有用这个6n做的模型发在很好的期刊上的文章，作者立马回去检查，
重复，确保表型和这个突变无关。
而这段时间的地震是RGC-5这个细胞系，大家也许都听过这个细胞，这个最开始是rat
ganglion cell line，但是呢，最近发现，这个细胞系其实没有在originator's实验室
外存在过，现在流传的RGC-5实际上已经mouse origin的cone photoreceptor cell
line。。。。这个很震惊，因为从最开始到现在已经过去了10多年的时间，用这个细胞
株发表的文章也超过了200多篇，也就是说，究竟有多少结论是站得住脚的，我们无法
得知了。
所以几个vision的journal都相继出社论来警告尤其用细胞系来做实验的，建议要用in
vivo或者pr... 阅读全帖 |
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E**********t
发帖数: 56 | 43 Xuebiao Yao, Dasheng Li and Gang Pei recently wrote an essay to Nature
Review in MCB Which is entitled “In focus: molecular and cell biology
research in China”. Here is the link: http://www.nature.com/nrm/journal/v14/n9/full/nrm3638.html In this article, they discussed the past, present and future of molecular and cell biology research in China. One part which interested me is the key MCB discoveries in China. Although most of the work are nice and likely solid work, few of them are breakthrough... 阅读全帖 |
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h*****G
发帖数: 113 | 44 方向：
human embryonic stem cell,
gene targeting
developmental biology
reprogramming 等领域
谢谢。 |
|
a******s
发帖数: 37 | 45 好像用的比较多的是129或者129xb6 f1的es line。不知道是否不是因为b6的germ line
transmission效率低？ |
|
r******g
发帖数: 600 | 46 Yes.
Crispr can make germline KO in B6 ES cells. |
|
D*a
发帖数: 6830 | 47 貌似不是的，129是最先用的，但是同样的protocol在B6上不好用，所以大家觉得B6效
率低。但是后来大家摸索出来适合B6的protocol了，B6效率就不低了。关键是用对
protocol
聊天听说的。 |
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r******g
发帖数: 600 | 48 B6 效率很低的
用129然后backcross效率好很多
并且founder mouse用crossbackground比较容易找啊 |
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h*******o
发帖数: 4884 | 49 既然有transgenic mouse strain了， 那就把colony 建立起来，然后原代培养
astrocytes和microglia就行了啊
这个不是adult neuron培养，容易多了
如果你不要adult的， embryonic的neuron也好养 |
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m******g
发帖数: 467 | 50 Science 14.05.02
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The mice that didn't show pain had been handled by male students
Rodents exposed to male odors were actually feeling less pain
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By cloning adult skin cells
3. Genomic Signatures of Specialized Metabolism in Plants
How algae and land plants ha... 阅读全帖 |
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