X***y
发帖数: 3947 | 1 这个是为什么？请详解：一般embryonic stem cell的产生方法是需要销毁胚胎的，但
是这些胚胎本来就是要被销毁的。 |
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E*****m
发帖数: 25615 | 2 歡迎反對幹細胞研究的基督徒評論， 尤其是那 99.99%
不知道 stem cell 怎麼回事的基督徒。
也歡迎非基不置可否， 尤其是那 99.99%
不知道 stem cell 怎麼回事的非基。 |
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b*****o
发帖数: 482 | 4 我都不知道基为什么要反干细胞研究． 神的律法里有这一条吗？ |
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b*****o
发帖数: 482 | 7 你这是ＧＡ（Ｇｏｄ ａｔｔａｃｋ）本版不允许的
上次有人问神有没有那个器官 就被封了． |
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b********n
发帖数: 38600 | 9 http://www.mindposts.com/uncategorized/tim-tebow-christian-tali
Superstar NFL quarterback Tim Tebow looks like an All-American guy, but he
has more in common with the Taliban than with our Founding Fathers.
Tebow has made a public spectacle of his religiosity. Genuflecting on the
field in a move that has become known as “Tebowing“. Filming a documentary
about his biblical indoctrination as a home-schooled boy by his missionary
parents. Proselytizing bible verses in his eye-black. Leading prayer... 阅读全帖 |
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l**********n
发帖数: 675 | 10 进化论在科学上的种种难题
一 进化论若干证据分析
（一）胚胎重演论
大多数高中生物学课本中都有一张胚胎发育图，显示鱼、蝾螈、乌龟、鸡、猪、牛、兔
、人等动物在胚胎发育不同阶段的侧面图，其原版收藏在伦敦大学的Bodleian图书馆，
它的作者是十九世纪德国Jena大学的生物学教授海克尔（Ernst Haeckel）。从这张图
得出的结论是，虽然这些动物成年后形态各不相同，但是由于它们是由共同的祖先进化
而来，所以在胚胎发育过程中均有一个形态相似的阶段，这就是“胚胎重演论”（
Ontogeny recaptulates phylogeny）。
可以用“日晷模型”（Hourglass model）来阐述胚胎重演论，其横轴显示进化过程中
形态上的差异，纵轴显示胚胎发育时期，就象日晷一样，胚胎早期都是受精卵，胚胎发
育成熟后，就成了各种形态各异的动物，也就是说，不管这些动物发育成熟后形态上的
差异有多大，但在胚胎发育的某个阶段，其形态仍旧会重演一个类似鱼的形态。这个理
论和模型的价值在于，它提示在胚胎发育的过程中，残留物种进化的痕迹。这个理论强
有力地支持了进化论，并且作为进化论的一个主要证据，... 阅读全帖 |
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d********i
发帖数: 113 | 11 全文转载如下。
－－－－－－－－－－－－－
起初神创造－评进化论的由来与谬误
“起初神创造诸天与地”（创一1），这是圣经开宗明义的第一句话，这也是阿波罗八
号登月宇宙飞船上的宇航员首次到达月球外围时，发到地球上的第一个讯息。
当人观赏宇宙的伟大、恢宏、规则、精确，不能不想到其设计者；当人细察地球上亿万
生物的奇姿多彩、变化万千和巧妙配搭，也不能不联想到生命的源头和创始者（徒三15
）；当人站在镜子前观看自己身体构造的奇妙，或在夜深人静时思想内心世界的丰富和
良心感觉的可畏，不能不赞叹“因我受造奇妙可畏；你的作为奇妙，这是我心深知道的
”（诗一三九14）。
然而，自一八五九年达尔文发表《物种起源》（The Origin of Species）（这是达尔
文所提出的解释宇宙、生命和人起源的假说），提出进化学说之后，进化的思想就如野
火蔓延，甚至写进许多国家的教科书里。因着进化论的教育，许多人误把进化论当作科
学真理，而把圣经中所启示的“起初神创造”当作不合科学的神话。这一进化学说蒙蔽
人的心思，拦阻人对宇宙创造者和宇宙存在意义的追寻，贬低人生存的意义和价值，导
致社会道德的退化。
本文通过... 阅读全帖 |
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w**u
发帖数: 311 | 12 SeeU,
How do you think of biologist killing mice to understand the truth of life?
In 2007, the Noble Prize winners (Mario R. Capecchi, Sir Martin J. Evans,
Oliver Smithies) for physiology are awarded "for their discoveries of
principles for introducing specific gene modifications in mice by the use of
embryonic stem cells". I am using this technology to tackle the biology
questions related to human disease and need kill lot of mice.
Runsun has answered my question before. But would you please a |
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i*e
发帖数: 859 | 13 The Nobel Assembly at Karolinska Institutet has today decided
to award The Nobel Prize in Physiology or Medicine for 2002
jointly to Sydney Brenner, H. Robert Horvitz and John E. Sulston
for their discoveries concerning "genetic regulation of organ
development and programmed cell death".
Summary
The human body consists of hundreds of cell types, all
originating from the fertilized egg. During the embryonic and
foetal periods, the number of cells increase dramatically. The
cel |
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M****e
发帖数: 70 | 14 for the offspring from 1st cousin, the possibility getting
homozygous mutations (derived from one of the grandparents)
is only 1/64. and for the reason that uteral or embryonic
lethality increases with homozygous recessive mutations, the
observed genetic disorders could be underestimated. for the
research per se, i have not read the paper. but i would like
to point out that genetic background and subpopulation
structure must be taken account to interpret the data. otherwise,
it could be easily b |
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M****e
发帖数: 70 | 15 a group from UK has applied genome-wide RNAi and addressed
the effects of gene silencing in C. elegans. Basically,
they PCR and generated targetting constructs for about
16,757 of predicted C. elegans genes. The bacteria library
was fed to hermaphrodites and RNAi effects were analyzed as
"nonviable", "growth defects" and "viable post-embryonic
development", etc.
Refer to Nature 421:231 and 421:268 as well.
Comments by Tom Tuschl Nature 421:220
(it seems that he is now at Rockefeller).
btw, assas |
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b*********t
发帖数: 877 | 16 在这里帮你解答算不算作弊啊，呵呵。
先声明我其实也不是专搞这个的。以前学了一点毛皮而已。你要是去看点early
embryonic dev.的大牛如Elizabeth Roberson 跟Bridget Hogan的文章就会知道是该怎么
答了。
答案一：cell non-autonomous。 这就是说这个yfmg either 是个secreted factor
itself, or is upstream in a pathway that results in the secretion of other
important factors.
但是要test this hypothesis through chimera expt好象不容易。我还没想出来。
答案二：这个yfmg is involved in the development of the trophoblasts
, which do NOT contribute to the actual embryo proper. 你的ES cells
injected get integrated into the inn |
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p*****n
发帖数: 981 | 17 ☆─────────────────────────────────────☆
cmv (cmv) 于 (Sat Feb 3 12:41:19 2007) 提到:
embryonic or adult
Thanks !
☆─────────────────────────────────────☆
lovelysp (lovelysp) 于 (Sat Feb 3 13:38:35 2007) 提到:
too many to define.People start to believe almost any tissue have rare stem
cells.
☆─────────────────────────────────────☆
NatureCell (CncerCell) 于 (Sat Feb 3 17:27:55 2007) 提到:
Fuchs, E (HHMI)
Weissman,IL
☆─────────────────────────────────────☆
prosaic (jimmy) 于 (Sun Feb 4 |
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d*****r
发帖数: 2583 | 18 this issue of Cell, two beautiful papers by computational neuroscientists:
I think this is the True "systems Biology" in my heart...
Stability and Nuclear Dynamics of the Bicoid Morphogen Gradient
T. Gregor, E.F. Wieschaus, A.P. McGregor, W. Bialek, and D.W. Tank
Probing the Limits to Positional Information
T. Gregor, D.W. Tank, E.F. Wieschaus, and W. Bialek
The structure of multicellular organisms is determined during early
embryonic development by spatially and temporally changing patterns of |
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l*****g
发帖数: 43 | 19 多谢本版俩同胞不吝相助,虽然实质上没有进展,但有新文章出来,从科学层面上是对我
的肯定,又有声鞑老印文章的架式,心结有很大的释放.
老印的文章是
REST maintains self-renewal and pluripotency of embryonic stem cells.
Nature. 2008 May 8;453(7192):223-7
新文章是
Development 136, 715-721 (2009) doi:10.1242/dev.028548.
Is REST required for ESC pluripotency? Nature doi:10.1038/nature07783
但可笑的是, NIH不肯进行调查,原因是MD ANDERSON说只是不同的解释,不是造假.我已
经打算改行,但心里不肯放过这老印.唯一能做的是等待NATURE给我的回答,已经等了快
三个月了,NATURE也接受了持相反结论的文章,迟迟不给我回音也不知道为什么.写给NIH
,是想铲除老印,他太坏了,害的中国人无数.
我给NATURE的数据很难看,当时是做给自己看的,哪想到要送NAT |
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d*****r
发帖数: 2583 | 20 ☆─────────────────────────────────────☆
lanfang (yang) 于 (Fri Feb 13 00:43:04 2009) 提到:
多谢本版俩同胞不吝相助,虽然实质上没有进展,但有新文章出来,从科学层面上是对我
的肯定,又有声鞑老印文章的架式,心结有很大的释放.
老印的文章是
REST maintains self-renewal and pluripotency of embryonic stem cells.
Nature. 2008 May 8;453(7192):223-7
新文章是
Development 136, 715-721 (2009) doi:10.1242/dev.028548.
Is REST required for ESC pluripotency? Nature doi:10.1038/nature07783
但可笑的是, NIH不肯进行调查,原因是MD ANDERSON说只是不同的解释,不是造假.我已
经打算改行,但心里不肯放过这老印.唯一能做的是等待NATURE给我的回答,已经等了快
三个月了,NA |
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h********n
发帖数: 4079 | 21 我做的这个基因是tumor suppressor and/or metastasis suppressor, 这个才是我做
的方向, 有好多老鼠的活要做.
embryonic lethal这种事有点难, 主要是我没啥development的经验. 先干着吧. 我本
来跟老板说我不想lead这个project, 现在估计只有我能lead, 除非老板再招一个人干
活, 我就可以把这个脱手了.
上个月刚刚写了一个新的关于cancer的animal protocol(实验室以前没有独立的animal
protocol), 还好以前phd的时候写过不少类似的. 现在又写一个development的
protocol, 这两个月都在写东西. 老板坚决不肯在animal protocol上出现他老人家的
名字, 所以这两个animal protocol的PI是我, 有啥事都是我的责任啊.... |
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s******r
发帖数: 2876 | 22 搭车问一声，这个tumor suppressor 和 metastasis suppressor怎么区别？
如果已经抑制tumor growth了，甚至导致tumor shrink了，tumor cell数目已经减少了
，那可转移的tumor cell也少了，不是也会导致metastasis减少了吗？
我做的这个基因是tumor suppressor and/or metastasis suppressor, 这个才是我做
的方向, 有好多老鼠的活要做.
embryonic lethal这种事有点难, 主要是我没啥development的经验. 先干着吧. 我本
来跟老板说我不想lead这个project, 现在估计只有我能lead, 除非老板再招一个人干
活, 我就可以把这个脱手了.
上个月刚刚写了一个新的关于cancer的animal protocol(实验室以前没有独立的animal
protocol), 还好以前phd的时候写过不少类似的. 现在又写一个development的
protocol, 这两个月都在写东西. 老板坚决不肯在animal protocol上出现他老 |
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m******5
发帖数: 1383 | 23 Gontan, C., T. Guttler, et al. (2009). "Exportin 4 mediates a novel nuclear
import pathway for Sox family transcription factors." J Cell Biol 185(1): 27
-34.
SRY and other Sox-type transcription factors are important developmental
regulators with various implications in human disease. In this study, we
identified Exp4 (exportin 4) as an interaction partner of Sox2 in mouse
embryonic stem cells and neural progenitors. We show that, besides its
established function in nuclear export, Exp4 acts |
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t******r
发帖数: 2 | 24 A post-doctoral position is available to investigate molecular pathogenesis
of childhood embryonal brain tumors at The Hospital for Sick Children/
University of Toronto, Toronto, Canada. The basic requirements include:
solid cell and molecular biology background; experience with high throughput
technologies, such as gene expression arrays, SNP arrays, miRNA arrays;
some basic informatic and statistical skills would be an asset.
The position is available immediately. Interested candidates please |
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m*p
发帖数: 226 | 25 Embryonic hearts were co-transfected by RFP and GFP plasmids. Only 20% were
transfected. Boss argued that
all RFP and GFP should co-localized and I said it is impossible, only when
RFP and GFP plasmids are in the
same lipid vesicle and fused with the same cell, then they will colocalize.
She said she was one thousand
percent sure. I can not say more, as this might harm the relationship.
However, I would like to know the truth about this co-transfection. Thanks
for your imput. |
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F***w
发帖数: 58 | 26 请好心人帮我下载一篇paper全文
Differential methylation of tissue- and cancer-specific CpG island shores
distinguishes human induced pluripotent stem cells, embryonic stem cells and
fibroblasts
Nature Genetics 41, 1350 - 1353 (2009)
信箱 b*****[email protected]
非常感谢！！！ |
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k****n
发帖数: 158 | 27 最近老板要我同步化MEF(mouse embryonic fibroblast) 在G2期然后释放（release）
，分析
细胞分裂（cell division）；
请问有什么效率比较高的protocol推荐吗，只考虑用化学试剂的方法来诱导--我认为这
样最省事；-
）。
当然在发帖之前我也google了一下，没有找到一个非常通用的方法，一些文献说用血清
饥饿法（serum
starvation）；我担心效率不会太高，而且好像同步在G1期，还有一些提到离心分离的
方法，这个对
我来说就太麻烦了。
最后找到下面的组合：
1）Thymidine-Nocodazole block
2）Aphidicolin and Hoechst
各位在做细胞分裂的大虾们，能不能提点下比较好的方法，或者帮我分析分析如上方法
是否可行？
不胜感激！ |
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j********r
发帖数: 156 | 28 要用gamma-ray 作 feeder MEFs for culture of embryonic stem cells. 大家推荐一个
protocol (such as dose and procedure, cells to be kept in pellet with ice
incubation or on tissue culture dish in antibiotics-free medium?) 多谢！ |
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s******y
发帖数: 28562 | 29 我用的是embryonic fibroblast, 和3T3差不多。
一般都能做到20%。 再高就没有办法了。
基本上，你要确认两件事：
1。你提的DNA足够纯。如果不放心的话，可以用ethanol/NaAc 沉淀法
纯化一次
2。细胞的状态。 必须在60~80% confluence 之间。
如果都不行的话，还有一个不是办法的办法，
那就是把细胞trpsinized, wash, resuspend, 然后在细胞贴壁之前
把转染混合物加进去。因为细胞在spread and adhere 的时候会吸进很多
vesicle.相当于就是强行把DNA弄进去了。不过这个方法会弄死很多细胞。
谢。 |
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s******y
发帖数: 28562 | 30 I assume you are talking about embryonic cardiomyocyte and skeletal muscle
cells.
In these cases, yes, they can contract on their own. |
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x**w
发帖数: 112 | 31 Nat Protoc. 2006;1(4):2026-36.
In vitro generation of germ cells from murine embryonic stem cells.
West JA, Park IH, Daley GQ, Geijsen N.
please send to n*****[email protected]
thanks a lot. |
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n********k
发帖数: 2818 | 32 Hi, folks:
Any of you do primary mouse neuron culture? I like to know which stage
one can easily derive primary
neurons, the later the better for me... We have protocols for embryonic
neurons but I'll like something later
than that but aslo need to be healthy and can tolerate with some
manipulation later on such as Cre-viral
infection.... |
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E*******2
发帖数: 131 | 33 我正在做ChIP-qPCR， anti acetyl Histone H3 and H4, 将来也会考虑做anti
methylation histones，现在遇到的问题
是，找不到合适的control gene primers. Millipore 公司提供的chip kits里用的
GAPDH。 但是我们领域有几篇文章是用
的beta globin和beta tubulin。我的老板说用我们领域已发表的。然后我查了一下他
们用的primer sequences，有意思的
是他们用的是小鼠，但是primer sequences和大鼠的基因是完全匹配的，而和小鼠有1
－2个碱基不匹配。并且beta
globin的primer在大鼠基因中扩增的是hemoglobin epsilone2，而在小鼠中最可能扩增
的是hemoglobin y， beta－like
embryonic chain。 我的问题是，如果我选择beta globin做为我的control gene，我
是否应该重新设计primer，还是说
我可以直接用人家发表的primer sequence？但是他们用的是sybr |
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j********r
发帖数: 156 | 34 The experiment is to isolate blastocyst for cloning mouse embryonic stem
cells. Can anyone give me a
protocol? I couldn't find any on the web. Also I was told to start with 21
days mice, and to mate them on day
23 after stimulating the females with hormones (PMS and hCG). Are they too
young?
Thanks a lot! |
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c**a
发帖数: 94 | 35 需要从embryonic mice brain 里分离并培养 endothelial cells. 求protocol.
会不会embryo脑子里血管不够多,不容易培养? |
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t**u
发帖数: 7 | 36 Postdoctoral research positions are available in the Neonatal Research
Program (Division of Newborn Medicine and Genetics) at Brigham & Women’s
Hospital, Harvard Medical School. The research project is devoted to
studying genomic stability in embryonic and induced pluripotent stem (ES and
iPS) cells. The goals of the lab are to improve our understanding of
pluripotency, gain insight into human development and help realize the
therapeutic potential of pluripotent stem cells.
We are interested in |
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M******t
发帖数: 555 | 37 http://www.ncbi.nlm.nih.gov/pubmed/19266325
Methods Mol Biol. 2009;530:141-64.
Gene targeting in mouse embryonic stem cells.
Tessarollo L, Palko ME, Akagi K, Coppola V.
Mouse Cancer Genetics Program, NCI-Frederick, Frederick, MD, USA. |
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R*****w
发帖数: 447 | 38 Thank you very much, sunnyday!
I figured it out and found that pcDNA3.1 does have sv40 origin.
The following is some information from Wiki, in case someone else is
interested in this:
HEK 293 cells were generated in early 70s by transformation of cultures of
normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex
Van der Eb's laboratory in Leiden, Holland.
An important variant of HEK293 cell line is the 293T cell line that contains
, in addition, the SV40 Large T-antigen, that |
|
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i*********0
发帖数: 915 | 40 Does someone know the FACS analysis to quantify the NSCs in the embronic
stages?
It would be great if you can share me some experiences.
Bests! |
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O******e
发帖数: 4845 | 41 希望不要出现什么差错。哪怕是很小的成功，都会有巨大的正面影响。
Business
in
clinical
trial |
|
S*********e
发帖数: 127 | 42 Hoch JR, Stark VK, van Rooijen N, Kim JL, Nutt MP, Warner TF. 1999.
Macrophage depletion alters vein graft intimal hyperplasia. Surgery 126:428
–37
Kennedy TE, Serafini T, de la Torre JR, Tessier-Lavigne M. 1994. Netrins are
diffusible chemotropic factors for commissural axons in the embryonic
spinal cord. Cell 78:425–35
Kolodkin AL, Matthes DJ, O’Connor TP, PatelNH, Admon A, et al. 1992.
Fasciclin IV: sequence, expression, and function during growth cone guidance
in the grasshopper embryo. Neur |
|
s******s
发帖数: 13035 | 43 embryonic germ cell and spermatogonial stem cell,
有什么细胞库有么？还是直接问作者要？ |
|
s****n
发帖数: 4902 | 44 Obama's Stem-Cell Policy Is Barred by Federal Judge
(From Wall Street Journal)
By BRENT KENDALL And LAURA MECKLER
WASHINGTON—A judge Monday blocked the federal government from funding
research involving human embryonic stem cells, a surprising blow to one of
the Obama administration's most significant science-policy initiatives.
In this May 1, 2008 file photo, U.S. District Judge Royce C. Lamberth is
seen during a ceremony at the federal courthouse in Washington.
Judge Royce Lamberth of U.S. Dis |
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p*****m
发帖数: 7030 | 45 小小的district judge而已 而且他等于是在要求两院推翻之前那个限制stem cell
funding的bill呗 hoho
Obama's Stem-Cell Policy Is Barred by Federal Judge
(From Wall Street Journal)
By BRENT KENDALL And LAURA MECKLER
WASHINGTON—A judge Monday blocked the federal government from funding
research involving human embryonic stem cells, a surprising blow to one of
the Obama administration's most significant science-policy initiatives.
In this May 1, 2008 file photo, U.S. District Judge Royce C. Lamberth is
seen during a ceremony at t |
|
D*********t
发帖数: 370 | 46 U.S. Judge Rules Against Obama's Stem Cell Policy
By GARDINER HARRIS
Published: August 23, 2010
WASHINGTON — A federal district judge on Monday blocked President Obama's
2009 executive order that expanded embryonic stem cell research, saying it
violated a ban on federal money being used to destroy embryos.
The ruling came as a shock to scientists at the National Institutes of
Health and at universities across the country, which had viewed the Obama
administration's new policy and the grants prov |
|
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s******e
发帖数: 370 | 48 we use a digestion kit from Zygem for embryonic tissue, it does save a lot of time |
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a*******g
发帖数: 162 | 49 想在Her2 tg mice里DELETE掉一个gene, KO embryonic lethal, 要conditional ko.
请问用ff tg 和 ff cre tg做对照可以吗？这样BREEDING起来快。刚开始做BREAST
CANCER，属于菜鸟，多谢指教！ |
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