e*******c
发帖数: 1479 | 1 我发现自己做的蛋白可能受到cap protein eIF4E or eIF4G的调控， 理论上来说这个
蛋白的mRNA应存在5'的TOP结构，就是5-15个聚嘧啶之类的，
但具体如何判断呢? 是以atg为起始密码往上游算起吗？多少的范围呢？
请各位大拿赐教。谢谢 | m******5
发帖数: 1383 | | l**********1
发帖数: 5204 | 3 not so simple if that region 5' UTR is not cleared
just try go to
//www.ncbi.nlm.nih.gov/pmc/articles/PMC2717535/
or
//archiv.ub.uni-heidelberg.de/volltextserver/volltexte/2009/8939/pdf/thesis.pdf
so might need NMR analysis
with below software link:
//bmm.cancerresearchuk.org/~3djigsaw/
Ps: if you already knew that region likes:
//plexdb.org/modules/PD_probeset/annotation.php?genechip=Barley1&sequence_id=Barley1_05933
just use
//go.princeton.edu/cgi-bin/GOTermFinder/GOTermFinder
or others from
//www.princeton.edu/quantbio/data-tools-resources/bioinformatics-tools-reso/
【在 e*******c 的大作中提到】
: 我发现自己做的蛋白可能受到cap protein eIF4E or eIF4G的调控， 理论上来说这个
: 蛋白的mRNA应存在5'的TOP结构，就是5-15个聚嘧啶之类的，
: 但具体如何判断呢? 是以atg为起始密码往上游算起吗？多少的范围呢？
: 请各位大拿赐教。谢谢
| e*******c
发帖数: 1479 | 4 哥们, 能否详细说说, 我觉得是否应该做核糖体分级分离, 然后对分离的mRNA进行RT-
PCR鉴定， 然后再作 NMR分析？
但是如何分析NMR结果？ | l**********1
发帖数: 5204 | 5 >能否详细说说, 我觉得是否应该做核糖体分级分离, 然后对分离的mRNA进行RT-
Yes, next steps are:
+1 Protein production
+2 Structure determination by X-ray synchrotron radiation,
Protocol please refer:
//uit.no/publikum/prosjekter/prosjekt?p_document_id=228973
----
然后再作 NMR分析interaction between cap and elF4E/G
if you already read 1H NMR spectra chemical shift value before
you can understand below PhD thesis:
//info.ifpan.edu.pl/~annan/dane/zasob/doktorat.pdf
its. Chapter 3. 7. 2 NMR Data Analysis at 298K
Equations 3.64 and 3.65
if you are freshman to 1H NMR spectra reading,
please firstly read below book
//www.chem.ox.ac.uk/spectroscopy/nmr/PDFs/Organic%20NMR%201.pdf |
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