e********r 发帖数: 147 | 1 怀疑有个基因的蛋白表达被调控是因为在翻译启始或翻译中间过程中,核糖体与mRNA的
结合效率受到影响。如果检查这个结合效率的话,除了用polyribosome assay外,一般
还可以用别的什么办法相互映证一下下?谢谢! | g********4 发帖数: 4959 | 2 in vitro translation, comparing with the mutation of potential cis-element.
For these assay, you can use reporter constructs.All constructs also can be
used for in vivo assay if necessary. |
|