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Biology版 - How to do brdU staining?
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问题:BrdU和TUNEL的prok用量MTT 和 [3H]thymidine
相关话题的讨论汇总
话题: staining话题: brdu话题: antibody话题: do话题: use
进入Biology版参与讨论
1 (共1页)
s********g
发帖数: 20
1
Can anyone please share a protocol for staining cell culture? And which
antibody to use?
I've tried several times but the background is very high. I can see staining
even without added BrdU :(
h****g
发帖数: 439
2
we use an antibody from roche. Because that antibody is mouse anti-mouse, so
we use MOM kit for staining. It's perfect!

staining

【在 s********g 的大作中提到】
: Can anyone please share a protocol for staining cell culture? And which
: antibody to use?
: I've tried several times but the background is very high. I can see staining
: even without added BrdU :(

y*******3
发帖数: 71
3
I used ABD serotec: MCA2060, rat anti brdu.
you need to treat your sample first by 1N HCL followed by 0.1 M boric acid (
pH8.5).
1 (共1页)
进入Biology版参与讨论
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MTT 和 [3H]thymidine神经干细胞与学习和记忆 六.抽丝剥茧 下
有没有可能:percentage of Brdu positive cells increased, while phospho-H3 positive cells didn't change神经干细胞与学习和记忆 七.踏上征途 完
薄后刚开工做个最简单的实验被笑话了,大家帮看看请教细胞培养高手和干细胞分化的内行(adipocyte differeniation)
好像这两篇文章在打擂台,懂行的来看看呀问题:BrdU和TUNEL的prok用量
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Re: 变态老师要求用EB染胶求教版上大牛,怎样区分clump 里的细胞是分裂来的还是自发地凑在一起的?
神经干细胞与学习和记忆 五.抽丝剥茧 中用Cleaved caspase-3抗体做IF-P
相关话题的讨论汇总
话题: staining话题: brdu话题: antibody话题: do话题: use