s********g
发帖数: 20 | 1 Can anyone please share a protocol for staining cell culture? And which
antibody to use?
I've tried several times but the background is very high. I can see staining
even without added BrdU :( |
h****g
发帖数: 439 | 2 we use an antibody from roche. Because that antibody is mouse anti-mouse, so
we use MOM kit for staining. It's perfect!
staining
【在 s********g 的大作中提到】
: Can anyone please share a protocol for staining cell culture? And which
: antibody to use?
: I've tried several times but the background is very high. I can see staining
: even without added BrdU :(
|
y*******3
发帖数: 71 | 3 I used ABD serotec: MCA2060, rat anti brdu.
you need to treat your sample first by 1N HCL followed by 0.1 M boric acid (
pH8.5). |
