h******u
发帖数: 602 | 1 检测一单链DNA是否形成HAIRPIN， 是不是Nuclease S1就是做好的方法了？初步打算用
Cy3-label 5'end，然后用Nuclease S1处理，如果形成HAIRPIN的话，LOOP就会被降解
，产物RUN PAGE GEL。
从来没用过Nuclease S1,有啥要注意的吗？谢谢了！ | a***e
发帖数: 1010 | 2 try diluting your enzyme, decreasing your digestion temperature. | h******u
发帖数: 602 | 3 The one we currently have is from Promega nad the concentration is: 20–100u
/μl.
I plan to use 1 unit, 10 unit and 100 unit per reaction (0.5ug oligo) and
incubate on ice, room temperature and 37 C. Is this OK?
【在 a***e 的大作中提到】
: try diluting your enzyme, decreasing your digestion temperature.
| a***e
发帖数: 1010 | 4 sure, it is a good start. |
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