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发帖数: 27 | 1 they are basically the same. the principle of run-on is that you first
isolate the nuclei and remove most of the proteins, but still keep the
RNA polymerase associated with nascent transcribed target gene. then
when you add NTP and p32 labeled UTP, the TXN will run on and then all
the product will be labeled. it allows mapping of the start point from
the size fractionated products.
run-off means that TXN occurs to in vitro DNA fragments, which are
typically restricted fragments with the start si |
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