m****r
发帖数: 1904 | 1 “The expulsion of an embryo or fetus before it can live on its own. Common
use often distinguishes between abortion and miscarriage, the former being
defined as the deliberate and induced emptying of a pregnancy.”
“Unavoidable abortion or miscarriage may be caused by accident, infectious
disease, mental or physical stress and strain, or because of a general
organic weakness on the part of the mother.”
“Deliberately to induce abortion or miscarriage by artificial means,...... is
an act of high cr... 阅读全帖 |
|
G****e
发帖数: 11198 | 2 【 以下文字转载自 WaterWorld 讨论区 】
发信人: GoBlue (Wolverines), 信区: WaterWorld
标 题: The Darker Side Of God
发信站: BBS 未名空间站 (Sat Jul 12 13:10:33 2014, 美东)
The Darker Side Of God
If you ask Christians to describe their quasi-chosen god of worship,
you’ll often hear such descriptors as “wonderful” and “loving.” This
choice of selective designation seems commonplace within the Christian
community. In fact, most churches ignore the Old Testament all together so
that the members feel comfortable propagatin... 阅读全帖 |
|
h*****9
发帖数: 4028 | 3 有志于修仙的同学可以参考一下。哈哈。
【 以下文字转载自 Wisdom 讨论区 】
发信人: bdbd (kakali), 信区: Wisdom
标 题: hotfly 童鞋，我老今天决定传你元神出窍大法。
发信站: BBS 未名空间站 (Sun Jan 10 17:59:34 2010, 美东)
Robert Monroe's Method
In his book, Journeys out of the Body, Robert Monroe describes a complicated
-sounding technique for inducing OBEs. In part, it is similar to other
imagination methods, but it starts with induction of the "vibrational state"
. Many spontaneous OBEs start with a feeling of shaking or vibrating, and
Monroe deliberately induces |
|
c****t
发帖数: 19049 | 4 ScienceDaily (July 9, 2011) — For the past decade, researchers have tried
to reprogram the identity of all kinds of cell types. Heart cells are one of
the most sought-after cells in regenerative medicine because researchers
anticipate that they may help to repair injured hearts by replacing lost
tissue. Now, researchers at the Perelman School of Medicine at the
University of Pennsylvania are the first to demonstrate the direct
conversion of a non-heart cell type into a heart cell by RNA transfer... 阅读全帖 |
|
D*****r
发帖数: 6791 | 5 http://www-cs-faculty.stanford.edu/~uno/smullyan.html
[excerpted from This Book Needs No Title: A Budget of Living Paradoxes by
Raymund Smullyan (Englewood Cliffs, New Jersey: Prentice-Hall, 1980).
Copyright (c) 1980 by Raymond M. Smullyan; all rights reserved.]
I. The Modern Period
Once upon a time there was a universe. In this universe there was a planet.
On this planet there was virtually no laughter. Nothing like ``humor'' was
really known. People never laughed, nor jested, nor kidded, nor j... 阅读全帖 |
|
l**********t
发帖数: 5754 | 6
"you are distorting words. i am not mutation expert but here is some of my
humble opinion on this subject"
-- if you are not familiar with the subject, please don't start with an
allegtion about "distorting words", because I'm a biochem major so I know
what these terms mean in the context of biological science.
"mutation can be spontaneous mutation, or induced mutation. what we are
talking about here is presumably spontaneous mutation that has been
happening throughout the history of evolution"... 阅读全帖 |
|
b**d
发帖数: 7644 | 7 Robert Monroe's Method
In his book, Journeys out of the Body, Robert Monroe describes a complicated
-sounding technique for inducing OBEs. In part, it is similar to other
imagination methods, but it starts with induction of the "vibrational state"
. Many spontaneous OBEs start with a feeling of shaking or vibrating, and
Monroe deliberately induces this state first. He suggests you do the
following. First lie down in a darkened room in any comfortable position,
but with your head pointing to magn |
|
l**********2
发帖数: 307 | 8 Check the link below:
http://www.higheredjobs.com/institution/details.cfm?JobCode=175
Position Description
The School of Dentistry is seeking a Postdoctoral Scholar to study oral
bacteria-induced inflammatory immune responses in a dynamic well-funded
research group, the Center of Oral Health and Systemic Disease (OHSD)
University of Louisville. This three year, NIH-funded project, aims to
elucidate the molecular mechanism of a novel serine/theronine kinase in P.
gingivalis-mediated inflammation ... 阅读全帖 |
|
|
发帖数: 1 | 9 复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假
http://bbs.tianya.cn/post-free-5558181-1.shtml
复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假，曹明富作为世界
名校复旦大学的校友，居然出现这么多低劣的抄袭文章，真为复旦大学蒙羞！详情如下：
可以查到曹明富一共34篇文章，其中3篇和生物无关，9篇综述，非第一或者通讯作
者9篇，会议论文1篇，2篇文献搜索不到，剩下10篇论著，居然8篇存在一稿多投或者相
互抄袭：
1. 文章1抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过氧化
作用》
2. 文章2和9为同一文章，一稿两投。
3. 文章6和文章10抄袭1993年《茶叶科学》的文章《茶多酚的生学学作用》
4.文章31，33，34内容相互重复，多处投稿。其中，文章34的图8，图9，居然和文
章31的图2A，2B完全一致。
文章目录：
1、茶多酚类复合物对小鼠体内脂质过氧化的影响，《科技通报》，2000，V0L.16
N0.1
抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过氧化作... 阅读全帖 |
|
发帖数: 1 | 10 复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假
http://bbs.tianya.cn/post-free-5558181-1.shtml
复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假，曹明富作为世
界名校复旦大学的校友，居然出现这么多低劣的抄袭文章，真为复旦大学蒙羞！详情如
下：
可以查到曹明富一共34篇文章，其中3篇和生物无关，9篇综述，非第一或者通讯作
者9篇，会议论文1篇，2篇文献搜索不到，剩下10篇论著，居然8篇存在一稿多投或者相
互抄袭：
1. 文章1抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过氧化
作用》
2. 文章2和9为同一文章，一稿两投。
3. 文章6和文章10抄袭1993年《茶叶科学》的文章《茶多酚的生学学作用》
4.文章31，33，34内容相互重复，多处投稿。其中，文章34的图8，图9，居然和文
章31的图2A，2B完全一致。
文章目录：
1、茶多酚类复合物对小鼠体内脂质过氧化的影响，《科技通报》，2000，V0L.16
N0.1
抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过... 阅读全帖 |
|
发帖数: 1 | 11 复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假
http://bbs.tianya.cn/post-free-5558181-1.shtml
复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假，曹明富作为世
界名校复旦大学的校友，居然出现这么多低劣的抄袭文章，真为复旦大学蒙羞！详情如
下：
可以查到曹明富一共34篇文章，其中3篇和生物无关，9篇综述，非第一或者通讯作
者9篇，会议论文1篇，2篇文献搜索不到，剩下10篇论著，居然8篇存在一稿多投或者相
互抄袭：
1. 文章1抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过氧化
作用》
2. 文章2和9为同一文章，一稿两投。
3. 文章6和文章10抄袭1993年《茶叶科学》的文章《茶多酚的生学学作用》
4.文章31，33，34内容相互重复，多处投稿。其中，文章34的图8，图9，居然和文
章31的图2A，2B完全一致。
文章目录：
1、茶多酚类复合物对小鼠体内脂质过氧化的影响，《科技通报》，2000，V0L.16
N0.1
抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过... 阅读全帖 |
|
发帖数: 1 | 12 复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假
http://bbs.tianya.cn/post-free-5558181-1.shtml
复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假，曹明富作为世界名
校复旦大学的校友，居然出现这么多低劣的抄袭文章，真为复旦大学蒙羞！详情如下：
可以查到曹明富一共34篇文章，其中3篇和生物无关，9篇综述，非第一或者通讯作
者9篇，会议论文1篇，2篇文献搜索不到，剩下10篇论著，居然8篇存在一稿多投或者相
互抄袭：
1. 文章1抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过氧化
作用》
2. 文章2和9为同一文章，一稿两投。
3. 文章6和文章10抄袭1993年《茶叶科学》的文章《茶多酚的生学学作用》
4.文章31，33，34内容相互重复，多处投稿。其中，文章34的图8，图9，居然和文
章31的图2A，2B完全一致。
文章目录：
1、茶多酚类复合物对小鼠体内脂质过氧化的影响，《科技通报》，2000，V0L.16
N0.1
抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过氧化作用》... 阅读全帖 |
|
发帖数: 1 | 13 复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假
http://bbs.tianya.cn/post-free-5558181-1.shtml
复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假，曹明富作为世界名
校复旦大学的校友，居然出现这么多低劣的抄袭文章，真为复旦大学蒙羞！详情如下：
可以查到曹明富一共34篇文章，其中3篇和生物无关，9篇综述，非第一或者通讯作
者9篇，会议论文1篇，2篇文献搜索不到，剩下10篇论著，居然8篇存在一稿多投或者相
互抄袭：
1. 文章1抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过氧化
作用》
2. 文章2和9为同一文章，一稿两投。
3. 文章6和文章10抄袭1993年《茶叶科学》的文章《茶多酚的生学学作用》
4.文章31，33，34内容相互重复，多处投稿。其中，文章34的图8，图9，居然和文
章31的图2A，2B完全一致。
文章目录：
1、茶多酚类复合物对小鼠体内脂质过氧化的影响，《科技通报》，2000，V0L.16
N0.1
抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过氧化作用》... 阅读全帖 |
|
发帖数: 1 | 14 复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假
http://bbs.tianya.cn/post-free-5558181-1.shtml
复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假，曹明富作为世界名
校复旦大学的校友，居然出现这么多低劣的抄袭文章，真为复旦大学蒙羞！详情如下：
可以查到曹明富一共34篇文章，其中3篇和生物无关，9篇综述，非第一或者通讯作
者9篇，会议论文1篇，2篇文献搜索不到，剩下10篇论著，居然8篇存在一稿多投或者相
互抄袭：
1. 文章1抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过氧化
作用》
2. 文章2和9为同一文章，一稿两投。
3. 文章6和文章10抄袭1993年《茶叶科学》的文章《茶多酚的生学学作用》
4.文章31，33，34内容相互重复，多处投稿。其中，文章34的图8，图9，居然和文
章31的图2A，2B完全一致。
文章目录：
1、茶多酚类复合物对小鼠体内脂质过氧化的影响，《科技通报》，2000，V0L.16
N0.1
抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过氧化作用》... 阅读全帖 |
|
发帖数: 1 | 15 复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假
http://bbs.tianya.cn/post-free-5558181-1.shtml
复旦大学杭州校友会会长、博士生导师曹明富大量文章抄袭和造假，曹明富作为
世界名校复旦大学的校友，居然出现这么多低劣的抄袭文章，真为复旦大学蒙羞！详情
如下：
可以查到曹明富一共34篇文章，其中3篇和生物无关，9篇综述，非第一或者通讯作
者9篇，会议论文1篇，2篇文献搜索不到，剩下10篇论著，居然8篇存在一稿多投或者相
互抄袭：
1. 文章1抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）的抗脂质过氧化
作用》
2. 文章2和9为同一文章，一稿两投。
3. 文章6和文章10抄袭1993年《茶叶科学》的文章《茶多酚的生学学作用》
4.文章31，33，34内容相互重复，多处投稿。其中，文章34的图8，图9，居然和文
章31的图2A，2B完全一致。
文章目录：
1、茶多酚类复合物对小鼠体内脂质过氧化的影响，《科技通报》，2000，V0L.16
N0.1
抄袭1999年《中国兽医学报》11月文章《茶多酚（GTPP）... 阅读全帖 |
|
M*****8
发帖数: 17722 | 16 【 以下文字转载自 TraderSuccess 俱乐部 】
发信人: MB80528 (肥猫(Contrarian)[食MM而肥]), 信区: TraderSuccess
标 题: 今天 TraderSuccess(tm) 电子信的内容 (March 22, 2011)
发信站: BBS 未名空间站 (Wed Mar 23 13:02:39 2011, 美东)
有免费的 eMail Newsletter:
欢迎订阅。
http://StockCounsel.com/pm/user/subscribe.php
=================================================
MCP Now Bearish (March 22, 2011)
We now confirm that MCP is over-extended and bearish.
http://yhoo.it/eEiguu
Here is the latest forecasts (as of last night, March 22, 2011):
SYMBOL, DATE... 阅读全帖 |
|
wh
发帖数: 141625 | 17 【 以下文字转载自 Connecticut 讨论区 】
发信人: wh (wh), 信区: Connecticut
标 题: 下周一耶鲁有个关于河南黄河流域治理的讲座
发信站: BBS 未名空间站 (Fri Apr 6 16:56:12 2012, 美东)
时间地点介绍贴在下面。看上去是从抗战时期、蒋介石炸毁河南郑州花园口的黄河大堤
说起，到1941-43年的河南大饥荒。河南作家刘震云的中篇小说《温故一九四二》说的
就是那个事儿，大批河南人背井离乡逃难，还有很多人吃人的事件，惨不忍睹。建国以
后挖井、修渠，有个很出名的红旗渠。但现在黄河水一年比一年干，水渠的水也比以前
少很多。去年去河南，听当地人说因为挖井、修渠大量开发地下水，破坏了生态环境平
衡。这个讲座似乎还是持积极态度的。有关心这方面的朋友可以去听听。
Monday, April 9, 2012 (4:30 PM)
2011-2012 CEAS CHINA COLLOQUIUM SERIES
War, Water, Power: An Environmental History of Henan's Yellow River... 阅读全帖 |
|
f*********p
发帖数: 13 | 18 IPTG induces the expression of T7 polymerase (T7 pol is under the control of
lac, inducible by IPTG), which in turn translates your gene of interest
because it is under the control of T7 promoter and has to be processed by T7
pol, but not E.coli pol. without IPTG, T7 pol has very low basal expression
level, and your gene of interest will have some low basal expression too.
plysS expresses a low level of lysozyme, which functions as an inhibitor for
T7 pol, and thus, with no IPTG induction, inhib |
|
S******9
发帖数: 2837 | 19 你这个beta-actin Cre是non inducible的？
我们组研究的target基因就是在胚胎11.5～12.5天的时候死掉。即使是Tie2 Cre内皮特
异性的也是的。所以我们研究的成鼠是用的beta-actin Cre是inducible的，可惜我们
的flox做的不是特别好，有leaky现象，部分老鼠实际出生的时候已经有很大的ko了，
当然用药物诱导以后可以ko掉90%。
叫你老板把我招去吧，我喜欢做老鼠model的活。做atherosclerosis，vasoactivity，
原代血管内皮培养，各种组织切片染色。 |
|
a******u
发帖数: 211 | 20 Please send those papers to e*********[email protected]
thanks so much!!!!
1. Toxicol Mech Methods. 2009 Jan;19(1):51-8.
Silica-induced TNF-alpha and TGF-beta1 expression in RAW264.7 cells are
dependent on Src-ERK/AP-1 pathways.
Li X, Hu Y, Jin Z, Jiang H, Wen J.
2.Toxicology. 2006 Oct 3;227(1-2):105-16. Epub 2006 Aug 11.
Mechanisms of silica-induced IL-8 release from A549 cells: initial kinase-
activation does not require EGFR activation or particle uptake.
Øvrevik J, Refsnes M, Namork E, Bech |
|
T**********r
发帖数: 287 | 21
inducible
多谢提醒，不过你提到这个是openbiosystem的另外一个系统TRIPZ，的确是用
tetracyline
induce的。
Open biosystem有两种shRNAmir系统实现knock down:
a.Stable knockdown: GIPZ；shRNAmir is constitutively expressed as a
polycistronicvector that codes for tGFP, puromycin resistance
marker (Puror) and the shRNAmir.
b.Regulated knockdown: pTRIPZ,也是你提到的。
见链接
http://www.openbiosystems.com/collateral/rnai/pi/Lentiviral%20shRNAmir%2
0for%20Stable%20and%20Regulatable%20RNAi.pdf
再次真诚感谢，有了质疑，才能避免将来实验走弯路。 |
|
f******9
发帖数: 39 | 22 Neuropsychopharmacology. 2010 Jun;35(7):1550-9. Epub 2010 Mar 3.
Adenosine A1 receptors and microglial cells mediate CX3CL1-induced
protection of hippocampal neurons against Glu-induced death.
Lauro C, Cipriani R, Catalano M, Trettel F, Chece G, Brusadin V, Antonilli L
, van Rooijen N, Eusebi F, Fredholm BB, Limatola C.
PMID: 20200508
http://www.nature.com/npp/journal/v35/n7/full/npp201026a.html
Thanks in advance. Please send me to the email: f******[email protected] |
|
H*****e
发帖数: 120 | 23 Wow, on the top list!
Well, I never expect to be on the top list. This reason is that I am not
here very often. Also apologize to those are discussing about the topic and
expect me to give my opinion.
I believe that the previous title regarding to someone retract her paper is
misleading. So it is necessary to clear some issue and have an opportunity
to draw more discussion. Now back to the stem cell issue not the Amy or
Carla although name may be mentioned but please ignore my own personal
... 阅读全帖 |
|
y******8
发帖数: 1764 | 24 One sample from google. Good luck!
Dear PLoS Biology editor:
I would like to submit a presubmission inquiry regarding suitability of our
manuscript, "Subversion of cellular autophagy pathway by RNA viruses" by
William T. Jackson, Thomas H. Giddings Jr., Sara Mulinyawe, Ron Kopito and
Karla Kirkegaard for publication in PLoS Biology. Cellular autophagy is a
field that is just entering a period of rapid discovery, because the
mammalian homologs for the many yeast genes that affect autophagy have v... 阅读全帖 |
|
s******a
发帖数: 134 | 25 Could anyone please help me with some conditional KO questions?
Our lab have flox-transcription factor X-flox mice. I am thinking of
crossing these mice with some kind of
cre mice for conditional knockout of X . My purpose is to study the down
stream target of X. Specifically, I plan
to culture the cells in vitro , induce deletion of gene A in vitro, and to
examine gene expression between WT
and X KO cells. My questions are :
1) I hear that the efficiency of inducing conditional KO in vitro... 阅读全帖 |
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y******e
发帖数: 277 | 26 Nature Medicine 8, 808 - 815 (2002)
Published online: 15 July 2002; | doi:10.1038/nm735
Smac agonists sensitize for Apo2L/TRAIL- or anticancer drug-induced
apoptosis and induce regression of malignant glioma in vivo
Simone Fulda1, 4, Wolfgang Wick2, 4, Michael Weller2 & Klaus-Michael
Debatin1, 3
http://www.nature.com/nm/journal/v8/n8/abs/nm735.html
在家里，用不了校内paper权限。。。。
请发到
y*******[email protected]
多谢~麻烦啦 =） |
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g*********d
发帖数: 233 | 27 导读：治癌症，中国民间一直流传着以毒攻毒的说法，用小剂量的砒霜可治愈癌症。但
多少年以来，
人们对于砒霜为什么能够以毒攻毒却知之甚少，而新近上海血学研究所的专家却为世人
揭开了这层神
秘的面纱，从分子学的角度阐述了砒霜的作用机理。砒霜是中国民间自古以来就有的药
方，这也将为
中医药治疗疾病提供了西医的解读途径。
三氧化二砷俗称“砒霜”，经证实可用于治疗急性白血病，但“以毒攻毒”的分子机制
一直是个难题。我
国研究人员最近在这个领域取得重大突破，上海交通大学医学院附属瑞金医院上海血液
学研究所、医
学基因组学国家重点实验室等，近日在国际权威杂志《科学（Science）》上发表了“
三氧化二砷治
疗急性早幼粒白血病（APL）分子机制”的最新研究成果，研究揭示癌蛋白PML-RARα是
砷剂治疗APL
的直接药物靶点。
APL曾经被认为是最为凶险的白血病之一，很多患者在发病早期即死于严重出血，缺乏
有效治疗方
法。上世纪90年代，上海血研所陈竺、陈赛娟研究员等经过临床证实，全反式维甲酸和
三氧化二砷联
合应用，可以使约90％的APL患者达到5年无病生存。但三氧化二砷的直接分子靶点和分
子机理，... 阅读全帖 |
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l**********n
发帖数: 240 | 28 Josef Martin Penninger
Most significant scientific contributions:
Our basic approach is to genetically manipulate and change genes in mice and
to
determine the effects of these mutations in development of the whole
organism and in
diseases. From these mutations we are trying to establish basic principles
of
development and basic mechanisms of disease pathogenesis. My laboratory
focuses on
heart diseases, autoimmune diseases and cancers, and bone diseases. On all
the listed
contributions below I ... 阅读全帖 |
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G********e
发帖数: 528 | 29 I'm not confused either, but find quite a few studies by other people.
Either apoptosis induce H2AX phosphorylation or H2AX phosphorylation
regulate apoptosis.
J Biol Chem. 2000 Mar 31;275(13):9390-5.
Initiation of DNA fragmentation during apoptosis induces phosphorylation of
H2AX histone at serine 139.
DNA Repair (Amst). 2006 May 10;5(5):575-90. Epub 2006 Mar 29.
DNA-PK phosphorylates histone H2AX during apoptotic DNA fragmentation in
mammalian cells.
Molecular Cell, Volume 23, Issue 1, 121-132... 阅读全帖 |
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s**l
发帖数: 11983 | 30 http://asheducationbook.hematologylibrary.org/cgi/content/full/
Ham-Wasserman Lecture
Treatment of Acute Leukemia by Inducing Differentiation and Apoptosis
Zhen-yi Wang, MD*
Abstract
Conventional treatment of acute leukemia involves the use of cytotoxic
agents (chemotherapy), but other strategies have been explored. All-trans
retinoic acid (ATRA) and arsenic have clearly been effective in the
treatment of acute promyelocytic leukemia (APL), which creates the
possibility that other types of acute... 阅读全帖 |
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h********r
发帖数: 519 | 31 97-99 publications. NIU
Xianxin Hua, Zachary Miller, Geng Wu, Yigong Shi, Harvey F. Lodish (1999).
Specificity in TGF-beta-induced Transcription: Interactions of Promoter DNA,
Smad3 and TFE3. Proc. Natl. Acad. Sci. 96, 13130-13135.
Jie-Oh Lee, Haijuan Yang, Maria-Magdalena, Antonio Di Cristofano, Tomohiko
Maehama, Yigong Shi, Jack E. Dixon, Pier P. Pandolfi, and Nikola P.
Pavletich (1999). Crystal Structure of the PTEN Tumor Suppressor:
Implications for Its Phosphoinositide Phosphatase Activity ... 阅读全帖 |
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s*******e
发帖数: 1010 | 32 Do u have a cleavable His-tag on you protein? Sometimes protein (esp
membrane protein) has a low yield which can not be found by comparing
induced and non-induced samples.
If your protein is really important for you, try add a Thrombin, TEV or SUMO
protease cutable His-tag and do a simple separation with Nikel beads. Then
check if there is difference between SDS-PAGEs with and without protease
incubation. Good luck.
IPTG), spin |
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n*********b
发帖数: 140 | 33 Switching topics is a common technique for publishing in high profiled
journals. Here the authors switch one of defining features of iPSCs, the
ability to form teratoma.
Nowadays, inducing the formation of iPSCs is as easy as a high school
biology experiment, such as transforming bacteria. It is not surprised that
people simplify validation tests. Then, if induced ES cells fail to pass
the in vivo test, will you call it iPSCs even though they express all the
stem cell markers?
Publishing a p... 阅读全帖 |
|
x********u
发帖数: 430 | 34 I have constructed several gene circuits and got some unpredictable data (
Please open the link for the attached figure)
P: inducible promoter
P': constitutive promoter
X1 and X2: protein binding site (which will affect the expression level of
eGFP when the regulator protein binds them)
R1 and R2: putative regulator protein for sequence X1 and X2, respectively
eGFP: enhanced green fluorescence protein/reporter gene
A broad range of inducer concentration which known to bind effector R1 and
abolis... 阅读全帖 |
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h**********r
发帖数: 671 | 35 I have several questions about the experiment.
1). In the figures, do you use the same inducer?
2). The promoter, P has the similar function in the regulation system, X1-R1
and X2-R2?
3). R1 and R2 recognize the same inducers?
Thank you so much! |
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S******9
发帖数: 2837 | 36 是beta-actin的Cre？ 1084，1085，42，43 primers， Wt=300bp， Cre=100bp
才12只pups说明不了问题。
尤其是第一次生，总是litter size很小，后面就好一些。
你这个是inducible还是non-inducible的Cre? |
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g*****p
发帖数: 451 | 37 这个问题不难回答
从你以前的帖子已经知道你找到了酶被水解的位点
那你只需要合成一段相关的底物就可以了
然后还可以标记一下荧光，加点酶，看能不能切
要是能切的话，连kcat/km都可以算出来
如果能切，一般来说trans-leavage和cis-cleavage的认定才是
最头疼的事
一般来说得需要做个核磁或者晶体结构才行
要是你能拿到zymogen的结构呢，这个是比较好回答了
但你也说了这个酶不稳定，你想拿到zymogen的结构不做几个点突变把它稳定住
是不行的
不过现在为止你的实验数据有点让我困惑的地方（我不是说你的实验数据不对，只是说很
难解释这个现象，所以希望你先再确证一下）
1.你说4度放过夜，该酶都会变成truncated，这个和你开始express出来recombinant的
protease有两者混合的现象结合起来看，很难让我相信这个酶会有autocleavage的活性
因为要知道，ecoli表达重组蛋白，其细胞中的protease浓度是很高的。如果真的具有
自剪切活性，你根本不可能拿到full-length的protease,expression的时候就自己切光
了。因... 阅读全帖 |
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g*****p
发帖数: 451 | 38 哦，这样的话就很合情合理了
你开始的CHO分泌型表达，蛋白在培养基里面的浓度是很低的
一般表达良好的外源蛋白在CHO中最多也就1~10 mg/L，如果你的蛋白分子量在一般范围
20~50 kD, 那蛋白酶浓度也就0.02uM或者多点，况且pH比较高，酶的活性也低，所以你
能看到很多full length的酶，E.coli表达的东西都是包涵体，活性全无，自然也就大
都是
full length了
合成多肽切割实验不是证明有其他蛋白酶能切，而是证明它自己能识别这个位点，而且
可以
把动力学参数算出来，不过这个是反式剪切实验，至于你的自切是反式切割还是顺式切割
就没办法证明了
你说的pH induced 酶自切活性是个很有趣的发现
我觉得你得查查文献把这个酶的细胞定位搞清楚
因为在endosome pH5-6,如果酶定位在这里，那跟其生理功能是非常相关的
至少我知道的一个例子是流感病毒HA，它发挥膜融合功能的时候就是可以在endosome通过
pH induced构象变化，然后进行病毒－细胞膜融合
如果你的pH导致构象变化是有意义的
那做药就至少有3种途径了
1.catalytic site... 阅读全帖 |
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n********k
发帖数: 2818 | 39 This 肌无力 is not that 肌无力...when u have brain damage due to infection
and subsequently inflamation-induced damage, anything could happen dependent
on where the damage is. Based on ur early description, I think there is
at least a component of infection, which may be the sole cause or at least
the inducer if the girl does have a genetic lesion. For now, to prevent the
brain damage is more important than finding the cause. But I am not sure
that's still possible, since the symptoms say the damag... 阅读全帖 |
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y****y
发帖数: 123 | 40 山中当年在美国也是整天对着老鼠笼子默默流泪来的
后来回日本接着博后得了抑郁症要退的时候突然拿到那个二流大学的faculty
进去以后还整天被劝说做点有用的东西不好么
再后来...就搞大了
不过相比只需要转四种就可以reprogramming
我觉得最近说的ips其实不是induced的ps只是immortalized的ps比较靠谱
Multilineage-differentiating stress-enduring (Muse) cells are a primary
source of induced pluripotent stem cells in human fibroblasts.
当然不管怎样山中最后还是可能拿奖
但是ips会成为有史以来最大的生物泡沫也说不定 |
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D***y
发帖数: 693 | 41 如果研究signal transduction pathway B
如果gene A activates pathway B, let's say in 1 hrs. but gene A could
possibly activates other unknown pathways. Treating cells with A leads to a
certain cell phenotype.
knockdown an essential component of transduction pathway B blocks this
phenotype induced by A.
Can I say that the cell phenotype induced by A is due to activating this
transduction pathway? |
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i*****i
发帖数: 154 | 42 老大要找的质粒应该是叫STEMCCA
Stem Cells. 2009 Mar;27(3):543-9.
Induced pluripotent stem cell generation using a single lentiviral stem cell
cassette.
Sommer CA, Stadtfeld M, Murphy GJ, Hochedlinger K, Kotton DN, Mostoslavsky G.
Abstract
Induced pluripotent stem (iPS) cells can be generated using retroviral
vectors expressing Oct4, Klf4, Sox2, and cMyc. Most prior studies have
required multiple retroviral vectors for reprogramming, resulting in high
numbers of genomic integrations in iPS cells and limiting... 阅读全帖 |
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X******n
发帖数: 914 | 43 Current Medicinal Chemistry
Volume 17, Issue 3, 2010, Pages 254-263
Hypoxia-inducible factor-1 as regulator of angiogenesis in rheumatoid
arthritis - Therapeutic implications
Immunopharmacology and Immunotoxicology
Volume 33, Issue 3, September 2011, Pages 568-575
Prolyl hydroxylase and hypoxia inducible factor: Potential targets for
cancer therapy
Please send to x******[email protected]
10 baozi
Thanks |
|
p*****m
发帖数: 7030 | 44 Induced Pluripotent Stem Cell Core Facility
Guokai Chen, PhD, Director
Mission and Overview
The NHLBI iPSC Core Facility was created in 2011 as the main research site
focusing on the applications of induced Pluripotent Stem Cells (iPSC) within
the NHLBI. Our mission is to accelerate stem cell research in the Institute
by providing investigators consultation, technical services and training in
human pluripotent stem cell technology. Our facility provides training and
technical support in the main... 阅读全帖 |
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l**********1
发帖数: 5204 | 45 这下那个韩国BSO女 Mi-Sun Kim 的 博士学位都可能被取消吧
Article No.17
EMBO J. 2004 Apr 7;23(7):1598-608.
Transrepression by a liganded nuclear receptor via a bHLH activator through co-regulator switching.
Murayama A, Kim MS, et al and Kato S.
httq://katolab-imagefraud.blogspot.com/2012/01/transrepression-by-liganded-nuclear.html
如果以上这篇收入了 Mi-Sun Kim 的 博士学位论文的话 2012 EMBO J 公布撤稿的话
CV citation:
March, 2004, Doctor of Philosophy, Agricultural & Life Sciences, University
of Tokyo, Tokyo, Japan
Advisor: Dr. Shigeaki Kat... 阅读全帖 |
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F*Q
发帖数: 3259 | 46 Japanese Researcher Retracts 3 Articles in AHA Journals
Akio Kawakami, a well published lipid researcher at Tokyo Medical and Dental
University, has retracted 3 papers from AHA journals, including one article
in AHA’s flagship journal Circulation. News of the retractions was first
reported on Retraction Watch.
Two of Kawakami’s co-authors are well known researchers affiliated with
Harvard University and the Brigham and Women’s Hospital, Peter Libby and
Frank Sacks. Prior to his position in Tokyo... 阅读全帖 |
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z****u
发帖数: 1007 | 47 【 以下文字转载自 Neuroscience 讨论区 】
发信人: zhaohu (天外飞猪), 信区: Neuroscience
标 题: 用哪个cre 能特异knockout 某个gene 在periphery sensory nerv
发信站: BBS 未名空间站 (Sat Apr 14 03:11:06 2012, 美东)
不是做神经的，最近发现某个颌面部的干细胞分布和peripheral sensory nerve fiber
紧密相关。想找个inducible cre来在sensory nerve fiber里knockout掉一些gene.
请教该用啥cre比较好啊。最好是inducible cre. 多谢先了。 |
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h*******o
发帖数: 4884 | 48 有没有什么药物，刺激后能产生糖尿病
STZ-induced diabetes is a well accepted diabetic model, however, not sure
how suitable it is for your case.
STZ basically induces pancreatic islet damages.
The other model is high fat diet model.
有没有什么药物，刺激后能够加速新陈代谢
In vitro, yes a lot.
In vivo, not that I know of.
分析脂肪前体细胞的数量和功能(Gene A KO vs WT)
You got a very interesting mouse model.
If money is not an issue, I would quickly run a proteomic analyses in the
liver and probably the brain (since I work in neurosciences).
Also, s... 阅读全帖 |
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C*********u
发帖数: 811 | 49 STZ主要是induce type 1 diabetes
向楼主这种情况我觉得应该是induce type2 diabetes，所以我也觉得high fat diet比
较好
喂个几个月WT老鼠应该都变得超级胖，如果gene A真的在metabolism里面起作用的话，
那么highfat diet会更突出它的作用
至于preadipocyte的话，可以搞primary culture的preadipocyte，然后让他们分化，
看他们differentiation的能力 |
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g********0
发帖数: 6201 | 50 Insubordination in the Lab
05/23/2012 David Pittman
In two recent cases, post-docs have published data without their principal
investigator’s consent. Can this be prevented from happening again?
Jiasheng Diao was a post-doc at the Purdue University, working in the lab of
X-ray crystallographer Miriam Hasson on structure of the protein butyrate
kinase when Hasson passed away in 2006. As a result, the university gave
control of her protein structure data to her husband, Purdue biochemist
David San... 阅读全帖 |
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