U******u
发帖数: 5829 | 1 Terra Firma — A Journey from Migrant Farm Labor
to Neurosurgery
Alfredo Quiñones-Hinojosa, M.D.
http://content.nejm.org/cgi/reprint/357/6/529.pdf
“You will spend the rest of
your life working in the
fields,” my cousin told me when
I arrived in the United States in
the mid-1980s. This fate indeed
appeared likely: a 19-
year-old illegal migrant
farm worker, I
had no English language
skills and no
dependable means of
support. I had grown
up in a small Mexican
farming community,
where I began... 阅读全帖 |
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m***f
发帖数: 1622 | 2
扯淡呢，看看高最近两年发表的文章吧
CELL STEM CELL, JBC, STEM CELL, PNAS，都是很不错的
就这样还不能PROMOTION? 在美国都可以tennure了
1. Wu T., Wang H., He J., Kang L., Jiang Y., Liu J., Zhang Y., Kou
Z., Liu L., Zhang X., Gao S. (2011) Reprogramming of trophoblast stem
cells into pluripotent stem cells by Oct4. Stem Cells 2011 Feb 8.
[Epub ahead of print].
2. Kang L., Wu T., Tao Y., Yuan Y., He J., Zhang Y., Luo T., Kou Z.,
Gao S. (2010) Viable mice produced from 3-factor induced pluripotent
stem (iPS) cells through tetrapl... 阅读全帖 |
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A*******s
发帖数: 9638 | 3 有人认为是浪费时间和精力。
AAN: Stem Cells Show Promise in Mouse Model of ALS
THURSDAY, Jan. 10 (HealthDay News) -- Implantation of a neural stem cell (
NCS) fraction of induced pluripotent stem cells (iPSCs) shows promise in a
mouse model of amyotrophic lateral sclerosis (ALS), according to a study
released in advance of its presentation at the annual meeting of the
American Academy of Neurology, which will be held from March 16 to 23 in San
Diego.
Stefania Corti, M.D., Ph.D., from the University of Milan, ... 阅读全帖 |
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c***r
发帖数: 1132 | 4 这两天学到不少新知识，感觉现在干细胞在世界范围内的应用很广泛，不仅仅是中国（
中国因为监管方面的问题，可能做得更aggresive。欧洲/日本/美国这些地方，监管还
是相当严格的）。
推荐你看看Steven Cohen的paper，很多临床实例。
一下子收到的信息量太大，如果有记错的地方，还请见谅。如果没记错的话，他就做干
细胞美容，facelifting和乳房再造都做（不是他做的，就是别的某个人做的，我实在
不擅长记人名）。除皱的话，就是把干细胞注射到脸部，然后慢慢生成血管，
differentiation出脂肪，把皮肤撑起来，达到除皱效果。直接注射脂肪的话，后期有
时会被人体吸收，撑不起来。用干细胞，就不会有后期吸收的问题，组织长出来后会一
直在，达到长期除皱效果。用干细胞乳房再造原理也类似（这个治疗的一般都是切除了
乳房的乳腺癌患者，术前/术后照片和视频相当震撼，而且这个生成的是生理上真正的
自体组织（人造乳头也完全看不出/感觉不出是假的），就是真的，往胸部填硅胶隆胸
的跟这个比，就是浮云啊。）
Rogen Khouri，乳房再造（搞笑的是，有个大妈，一不小心，给造大了，结果还得... 阅读全帖 |
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c***r
发帖数: 1132 | 5 这两天学到不少新知识，感觉现在干细胞在世界范围内的应用很广泛，不仅仅是中国（
中国因为监管方面的问题，可能做得更aggresive。欧洲/日本/美国这些地方，监管还
是相当严格的）。
推荐你看看Steven Cohen的paper，很多临床实例。
一下子收到的信息量太大，如果有记错的地方，还请见谅。如果没记错的话，他就做干
细胞美容，facelifting和乳房再造都做（不是他做的，就是别的某个人做的，我实在
不擅长记人名）。除皱的话，就是把干细胞注射到脸部，然后慢慢生成血管，
differentiation出脂肪，把皮肤撑起来，达到除皱效果。直接注射脂肪的话，后期有
时会被人体吸收，撑不起来。用干细胞，就不会有后期吸收的问题，组织长出来后会一
直在，达到长期除皱效果。用干细胞乳房再造原理也类似（这个治疗的一般都是切除了
乳房的乳腺癌患者，术前/术后照片和视频相当震撼，而且这个生成的是生理上真正的
自体组织（人造乳头也完全看不出/感觉不出是假的），就是真的，往胸部填硅胶隆胸
的跟这个比，就是浮云啊。）
Rogen Khouri，乳房再造（搞笑的是，有个大妈，一不小心，给造大了，结果还得... 阅读全帖 |
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m********8
发帖数: 314 | 6 google一下，这篇论文的引用又继续增加了几百次，到1200多次了。
A role for CD95 ligand in preventing graft rejection” by D. Bellgrau et al.
— Nature 377, 630–635; 1995 |
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w**********9
发帖数: 242 | 7 1962年5月23日， Dr. Ronald A. Malt 在麻省总院做了世界上第一例断肢再植。
【Dr. Malt was 30 and the chief surgical resident at Massachusetts General
Hospital on May 23, 1962, when Everett Knowles, 12, was taken into the
emergency room. Everett had been hopping a freight train to go to a baseball
game, but when he was thrown against a stone abutment, his right arm was
ripped off cleanly at the shoulder.
Until then, surgeons had been able to reattach partly severed limbs and
restore function. But no one had success with l... 阅读全帖 |
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f******g
发帖数: 1003 | 8 Reprogramming human endothelial cells to haematopoietic cells requires
vascular induction
Nature
Generating engraftable human haematopoietic cells from autologous tissues is
a potential route to new therapies for blood diseases. However, directed
differentiation of pluripotent stem cells yields haematopoietic cells that
engraft poorly. Here, we have devised a method to phenocopy the vascular-
niche microenvironment of haemogenic cells, thereby enabling reprogramming
of human endothelial cells in... 阅读全帖 |
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v**********m
发帖数: 5516 | 9 这个wiki的引文包含了PBS的纪录片和nature上的due credit文章
Due credit
Nature’s podcast charts 12 landmark discoveries in the history of science.
17 April 2013
Article toolsPDFRights & Permissions
Even the greatest scientific discoveries come with an element of the mundane
. A humble paperclip was biophysicist Raymond Gosling’s choice. Late one
night in May 1952, in a chemistry lab in London, the PhD student wrapped DNA
around a paperclip to keep the molecule’s fibres stretched taut in front
of an X-ray source so ... 阅读全帖 |
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S*********e
发帖数: 127 | 10 Allogeneic T Cells That Express an Anti-CD19 Chimeric Antigen Receptor
Induce Remissions of B-Cell Malignancies That Progress After Allogeneic
Hematopoietic Stem-Cell Transplantation Without Causing Graft-Versus-Host
Disease
Jennifer N. Brudno, Robert P.T. Somerville, Victoria Shi, Jeremy J. Rose,
David C. Halverson, Daniel H. Fowler, Juan C. Gea-Banacloche, Steven Z.
Pavletic, Dennis D. Hickstein, Tangying L. Lu, Steven A. Feldman, Alexander
T. Iwamoto, Roger Kurlander, Irina Maric, Andre Goy, ... 阅读全帖 |
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a******r
发帖数: 786 | 11 suction blister grafting |
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t***n
发帖数: 121 | 13 1. Curr Pharm Biotechnol. 2015;16(11):966-82.
Title : Process Development Strategies in Plant Molecular Farming.
http://www.ncbi.nlm.nih.gov/pubmed/26343135
3. Curr Protein Pept Sci. 2013 Nov;14(7):568-79.
Title : Heterologous production of peptides in plants: fusion proteins and
beyond.
http://www.ncbi.nlm.nih.gov/pubmed/23968345
4. Expert Opin Drug Deliv. 2015 Apr;12(4):653-67. doi: 10.1517/17425247.2015
.974546. Epub 2014 Oct 28.
Title: Elastin-like polypeptide for improved drug delivery for... 阅读全帖 |
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发帖数: 1 | 14 J Heart Lung Transplant. 2016 Jun 24. pii: S1053-2498(16)30188-7. doi: 10.
1016/j.healun.2016.06.010. [Epub ahead of print]
Lung intragraft donor-specific antibodies as a risk factor for graft loss.
Visentin J1, Chartier A2, Massara L3, Linares G4, Guidicelli G4, Blanchard
E2, Parrens M5, Begueret H6, Dromer C2, Taupin JL7.
email: [email protected]/* */
thanks |
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|
发帖数: 1 | 16 J Heart Lung Transplant. 2015 Aug;34(8):1050-7. doi: 10.1016/j.healun.2015.
03.002. Epub 2015 Mar 20.
Late antibody-mediated rejection after heart transplantation: Mortality,
graft function, and fulminant cardiac allograft vasculopathy.
Coutance G1, Ouldamar S1, Rouvier P2, Saheb S3, Suberbielle C4, Bréchot N5,
Hariri S6, Lebreton G1, Leprince P1, Varnous S7.
my Email: [email protected]/* */
Thank you very much. |
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d********O
发帖数: 1196 | 17 Title: Grafting Reactions of (Hydroxyethyl)-cellulose During Emulsion
Polymerization of Vinyl Monomers
Chapter 18, pp 351–367
Chapter DOI: 10.1021/ba-1986-0213.ch018
Advances in Chemistry, Vol. 213
Thank you very much in advance! |
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w******e
发帖数: 38 | 18 Would you please send me the PDF file of this paper?
Preparation and Properties of Volume Expansion Type Blends of Plasticized
Polyvinyl Chloride/Starch Graft Polyacrylate Sodium
Tao, Liu; Jing-Xin, Lei; Xin-Yuan, Li. Polymer-Plastics Technology and
Engineering, Volume 46, Number 6, June 2007, pp. 569-573.
http://www.informaworld.com/smpp/content~db=all~content=a778978881
my e-mail: w********[email protected]
Many thanks |
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d********O
发帖数: 1196 | 19 Title: pH-sensitive starch hydrogels via free radical graft copolymerization
, synthesis and properties
Journal: Carbohydrate Polymers
2009 , 77 (3) 634-638
doi:10.1016/j.carbpol.2009.02.004
Please send to D********[email protected] , if this is more convenient for you.
Thanks a lot! |
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d********O
发帖数: 1196 | 20 Title:Versatile Grafting of Polysaccharides in Homogeneous Mild Conditions
by Using Atom Transfer Radical Polymerization
Biomacromolecules, 2006, 7 (7), pp 2154–2161
DOI: 10.1021/bm0601373
Please send to D********[email protected] , if this is more convenient for you.
Thanks a lot! |
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d****i
发帖数: 360 | 21 One paper please! Thanks a lot! E-mail: x**********[email protected]
Preparation and pharmacodynamics of stearic acid and poly (lactic-co-
glycolic acid) grafted chitosan oligosaccharide micelles for 10-
hydroxycamptothecin
Show Abstract Zhou, Y.-Y., Du, Y.-Z., Wang, L., Yuan, H., Zhou, J.-P.,
Hu, F.-Q. 2010 International Journal of Pharmaceutics 393 (1-2), pp. 144-
152 |
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d********O
发帖数: 1196 | 22 Title: Homogeneous grafting reaction of vinyl pyrrolidone onto chitosan
Volume 63 Issue 10, Pages 1321 - 1326
Please send to D********[email protected] , if this is more convenient for you.
Thanks a lot! |
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d********O
发帖数: 1196 | 23 Title: Synthesis of Graft Copolymers of Cellulose with 4-Acryloylmorpholine,
2-Methacrylamidopyridine and N-Phenylmethacrylamide, and Determination of
Some Sorption Properties
2007, Volume 44, Issue 9, pages 947 - 952
DOI: 10.1080/10601320701424214
Please send to D********[email protected] , if this is more convenient for you.
Thanks a lot! |
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d********O
发帖数: 1196 | 25 Title: GRAFT COPOLYMERIZATION OF CELLULOSE, CELLULOSE DERIVATIVES, AND
LIGNOCELLULOSE
Journal: Polymer Reviews
Volume 26, Issue 1, 1986, Pages 81 – 141
Please send to D********[email protected] , if this is more convenient for you.
Thanks again! |
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d********O
发帖数: 1196 | 26 Titile: Grafting Reactions of (Hydroxyethyl)-cellulose During Emulsion
Polymerization of Vinyl Monomers
Advances in Chemistry, Vol. 213 Chapter 18, pp 351–367
Chapter DOI: 10.1021/ba-1986-0213.ch018
Please send to D********[email protected] , if this is more convenient for you.
Thanks and Have a great day! |
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d********O
发帖数: 1196 | 27 Title: Degradation and grafting of hydroxyethyl cellulose during emulsion
polymerization
2004,124, 159-163
DOI 10.1007/b12161
Please send to D********[email protected] , if this is more convenient for you.
Thanks a lot! |
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b***e
发帖数: 1008 | 28 Title: Aliphatic polyester-grafted starch-like polysaccharides by ring-
opening polymerization
Author(s): Dubois P, Krishnan M, Narayan R
Source: POLYMER Volume: 40 Issue: 11 Pages: 3091-3100
LInk: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TXW-3WNVT63-2D
&_user=508790&_coverDate=05%2F31%2F1999&_rdoc=1&_fmt=high&_orig=search&_sort
=d&_docanchor=&view=c&_acct=C000025157&_version=1&_urlVersion=0&_userid=
508790
&md5=f537d1a8efcfd3bc6ab5ede71ccf6fbd
非常感谢！
please send to yon |
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q****e
发帖数: 3660 | 29 Interaction forces between particles containing grafted or adsorbed polymer
layers
Tharwat Tadros
Advances in Colloid and Interface Science
Volume 104, Issues 1-3, 1 July 2003, Pages 191-226
Thank you! |
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d*****h
发帖数: 1892 | 32 【作者】Kristen L. Moffat MSa, I-Ning Elaine Wang MSa, Scott A. Rodeo MDb
and Helen H. Lu PhD
【文题】Orthopedic Interface Tissue Engineering for the Biological Fixation
of Soft Tissue Grafts
【期刊名，年份，卷（期），起止页码】Clinics in Sports Medicine
Volume 28, Issue 1, January 2009, Pages 157-176
【全文链接】http://www.sciencedirect.com/science/article/pii/S0278591908000707
【求助者联系方式】c**********[email protected]
不好意思，麻烦了，多谢！ |
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b***e
发帖数: 1008 | 33 (1)Nanocomposites of Poly(lactic acid) and Surface-Grafted MgO Nanoparticles
Authors: Li, Yonghui; Sun, Xiuzhi Susan
Source: Journal of Biobased Materials and Bioenergy, Volume 5, Number 4,
December 2011 , pp. 452-459(8)
Publisher: American Scientific Publishers
link:
http://www.ingentaconnect.com/content/asp/jbmb/2011/00000005/00
?token=004417c96a66e67405847447b494a2f7a317b4255477921363375686f23d76fddf5c
(2)Soy Protein Polymers and Adhesion Properties
Author: Sun, Xiuzhi Susan
Source: Journal o... 阅读全帖 |
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c****n
发帖数: 21367 | 35 trial and error是现阶段人类科学进步的主要方法
飞行器设计我们只有靠风洞，试飞，摔飞机那也得试
汽车设计我们只有靠风洞，试车，安全性也靠把车往墙上撞
你有什么好办法？上面讲的这俩例子的基础还是物理学，材料力学等，
这都是上千年的学问。西医要在生物的基础上搞还原论，妄图逃过
系统论总结经验的阶段，你认为可能吗？
西医的方法论是彻底的错了，这是投入高产出少的根本原因。
就在西医里面来说，相比内科，外科手术就是典型的trial and error，
比如ACL reconstruction，第一批放进膝盖里面的artificial graft是什么？
尼龙绳！结果可想而知。后面改进也失败得一塌糊涂。至今有效的
修补还得靠人体(Pat. Tendon,Hamstring,Alograft)。但总体来说，
西医在外科方面取得的进步，那比内科、制药业要显著多了吧。
理论有什么用？用来总结，用来提高。但是要产生理论，首先要用正确
的方法论，研究实践对象。西医有什么还原论得到的理论（微观规律总结）
能指导人体这个系统的？不靠系统论行吗？ |
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c****n
发帖数: 21367 | 36 来自主题: ChineseMed版 - 中医和围棋 个人觉得，起点不能分中西。现有的病例资料，历史的总结，
能用的都要用上。治病不分门派，有容乃大，治好就行。
中西医的主要分别不在于手段，而在于积累和发展的方向。
西医外科和中医其实是很相似的，都是拿人不当人玩，西医尤甚。
ACL recon第一个尝试的人工代韧带居然是尼龙绳（准确的说是
纤维绳，实在太傻逼所以没推广，后来涤纶和Gore-tex推广了，
害惨了好几万人），查阅资料看到这个的时候瀑布汗流下。
这跟神农尝百草所需要的勇气差不太多...
但也唯有如此，才有进步。现代西医外科也都是野蛮操作，器材技术
是发展了，其理论理念比刮骨疗伤又能强多少。还是以ACL recon为例，
钉在腿骨上固定graft的钉子，手术大夫明说和walmart能买到的高档
合金浴室钉无区别，只不过是医疗器材厂出品，价格加俩零而已。
西药，内科疗法则是彻底的走了邪路。试图用“有效成分”去
解决问题。这个从系统概念上就是错误的。当“有效成分”是
一种组合，那又如何是好？更甚之，如果“有效成分”是一个抽象
概念，它还牵涉系统状态变量，“提纯”能得到啥？呵呵。
只要是从现有的基础出发，使用系统论的方法指导，就能推进 |
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c****n
发帖数: 21367 | 37 我从来都呼吁成立中医药监局，用现代的科学手段武装中医
当然这个为什么很难我也说过了，不是技术上的问题。
没人逼你坐实验性的飞机，也没人逼你用中医，对吧。
19世纪初飞跃大西洋的航班现在绝对拿不到准航证，不也飞了？
客人也坐，对吧。
更直接的例子
西医做ACL recon的手术，拿碳素编织带和化纤（Gore-Tex）往人
膝盖里塞，导致上万人残疾，不叫“自认为理论正确，就让老百姓用”吗？
可就这么弄，西医外科才比内科发展快无数倍。
ACL recon的历史可以从这里翻到，synthetic graft的悲剧你可以
去问任何一个美国的knee specialist。这可是1980年以后的事情。
http://www.maitrise-orthop.com/corpusmaitri/orthopaedic/87_colombet/colombetus.shtml
你现在说什么中医这么做不对，比起来中医够温和的了吧。
为什么西医就可以这么做，中医这么做，还有一个统一的模型指导
使得数据利用率很高，你就反对呢？ |
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j***h
发帖数: 4412 | 38 RISD的学习虽然辛苦但尚属顺利，5月底开始放暑假，计划回国。强烈地觉得应该找份
实习的工作
sweetbuddy 发表于 2009-2-28 12:39
想暑假回北京实习，大家有好的建议吗？
潜水很久了，时常过来看看大家伙都在做什么 ~
RISD的学习虽然辛苦但尚属顺利，3月春假的时候会努力写一份学习总结出来，希望有
助大家选校
有个问题或许问得不是很对地方，但我知道的也只有这儿了
5月底开始放暑假，计划回国。强烈地觉得应该找份实习的工作
这才发现自己一片茫然，连北京有哪些建筑公司都不晓得，百度之后还依然茫然.
希望有相关经验的同学指点迷津,非常感谢！
最後の使徒 发表于 2009-2-28 13:06
你是建筑来着吧？
设计院有中建国际、建设部院、北京院、清华院
私企有五合、urbanus、MAD、非常
外企的话OMA, Steven Holl, Alsop, GRAFT, Arup在北京都有办公室，KPF好像有？记
不清
了……
另外东三环附近还有数不清的外来设计公司……
北京的设计公司很多的，应该不难找
说来4月底朱培不是去你们那么，你直接去找他套不就好了 |
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y****u
发帖数: 50 | 39 what is amphiphilic side chain? do you mean amphiphilic block copolymer
grafted onto the backbone of a polymer chain? I don't know anything about such
kind of material, but it is technically possible.
what do you mean by "all the side chains attached to one side of the polymer
backbone"? do you mean 100% isotactic? that makes mission impossible.
if there is a way to synthesize your material, there will be a way to tailor
the content of each component. |
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y****u
发帖数: 50 | 40 what is PEI?
first you need to determine the UV crosslinkable group to use
then you need to graft the functional group to PEI. |
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t*******n
发帖数: 66 | 41 Acording to my opinion, about one third of the paper in chemsitry is related
to the polymer to soem extent. The supermolecualr strcuture intitaed by block
or graft copolymer can be a great impact in teh future applciaiton in
optical, electronic and catalytis. The self -assembly with the polyemrs is
also a kind of promising research. |
|
m***n
发帖数: 266 | 42
artificial vascular graft
的研究由来已久。看这段中文我猜大致意思是biodegradable
polymer作scaffold, 即3毫米ID的channel, 用“多微孔的膜”创造一个ECM，
这个"多微孔的膜“的膜估计也是porous polymer, hydrogel之类的，然后把EC注入，
不懂”肝脏或者肾脏的细胞注入“的具体理由，大概就是多提供点cell吧。
我想他最后还要把硅晶片抽出来。
光看中文科技消息，看不出来这个除了用个硅晶片外有什么闪光点。 |
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c*******n
发帖数: 1648 | 43 Just read the story in C&E but I don't have online resources of NATURE. It has
sth to do with pressure phase diagram. However, it's interesting to see that
the molded samples are transparent. What does the "core-shell" mean in the
paper? Something like micron size particles in HIPS, but smaller? Is it
poly(butyl acrylate) grafted with polystyrene? Microemulsion polymerization?
I have read her similar paper, but that's not something new, just
Todt for a block copolymer. It seems that this time, s |
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b**s
发帖数: 589 | 44 推荐一篇文章，注意是IEEE的杂志
http://www.spectrum.ieee.org/WEBONLY/publicfeature/dec02/skin.html
(点击see illustration可以看到很漂亮的图片)
文章分析了Advanced Tissue Sciences Inc， Organogenesis走向chapter 11的故事
，我看完之后的感想是 tissue engineering 是一个据我所知最集大成的学科或生意，要
想成功，就看谁的思维更aggressive了。
比如我看到这句话，让困扰了我很长时间的问题得到了解答---“The grafts start
out as donated infant foreskin tissue taken from circumcisions. ”
我的建议是最好集中在某一个具体的产品，比如人造皮肤，比搞纯的平台技术要有前
途一些 |
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c*s
发帖数: 2145 | 45 so far, most papers I read on this topic involves polymer, not co-polymer.
Most commonly used method is either ATRP or surface grafting of MAA, NIPAM or
VP. due to their responsibility to temperature, pH or metal ions.. |
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h***s
发帖数: 111 | 46 对于直链高分子（不包括graft, dentric and comblike polymer;)，除了用 mmol/g 表
示外，下面那个定义相对好点
number of functional groups per chain?
fraction of functionalized polymer??
Thanks! |
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D********g
发帖数: 533 | 47 hehe~ en~ dip-coating和spin-coating的确差别很大。我只是随便说说我的想法，给
你参考一下而已；本人也是一个菜鸟，说的不对的呢，也请海涵。没有和您老抢饭碗的
意思。。。我们的样品polymer和substrate的作用力挺大的，和你的过程不大一样。
dip-coating的最大的问题是膜的厚度不如spin-coating的均一，对于我们的样品来说
不是很重要，因为还要graft第二层膜。我个人的感觉我现在做到dip-coating的时候，
不是很严苛的除尘。感觉上dust对electro-spin coating可能影响很大。不知道你现在
的步骤是不是这样~
jz |
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s*****i
发帖数: 146 | 48 【 以下文字转载自 Chemistry 讨论区 】
发信人: shaxixi (stupid), 信区: Chemistry
标 题: ask for a paper
发信站: BBS 未名空间站 (Tue Nov 21 07:19:06 2006)
Graft copolymers that exhibit temperature-induced phase transitions over a
wide range of pH
Guohua Chen & Allan S. Hoffman*
Center for Bioengineering, FL-20, University of Washington
thank you very much |
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y***e
发帖数: 6082 | 49 monomer A and monomer B 参与共聚的活性和空间位阻差别挺大的，solution-based
polymerization已经证明两者能共聚，现在引入到surface体系作surface grafting，
反应完了用什么方法或表征手段证明那个惰性单体参与了共聚呢？IR可以看官能团，但
很难说明惰性单体是chemical bonding上去的，还是non-specific adsorption给吸在
表面的呢？还有些具体情况，俺在回帖讨论中慢慢说明 |
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w********h
发帖数: 12367 | 50 什么surface?
用不影响surface的溶剂清洗或者回流抽提可溶解的未graft的部分，
然后ATR或者别的表面表征技术测测。。。 |
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