c*******e
发帖数: 4642 | 1 不一样的
国内是dry virus from rabbit
这里是from chicken embryo cell
大概是专利
全美国2008年，只有2例人类感染，还是被野生动物咬的 |
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p***d
发帖数: 2171 | 2 细长还敢做广告? 夜心说过, 关键要...
给你们个关于xxxxx size和身高, 脚长的研究, 夜心尤其要仔细研读一下.
http://www.springerlink.com/content/j62g2g4782873082/fulltext.pdf
One study found a weak correlation between the size of the stretched xxxxx
and foot size and height.
A potential explanation for a link is that the development of the xxxxx
in an embryo is controlled by some of the same Hox genes (in particular
HOXA13 and HOXD13) as those that control the development of the limbs. |
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c****n
发帖数: 21367 | 3 卵子冷冻出问题的概率可不小，不要迷信啊，现代科技没那么牛 :)
还不说取卵子那个手术所用的激素足够让女性折腾一段时间了，呵呵。
加一个科普性质新闻的link
http://www.cnn.com/2011/10/06/health/fertility-eggs-embryos-emp
Shapiro at RBA estimates that a woman who freezes her eggs between the ages
of 32 and 35 will have a 40 to 50% chance of achieving a successful
pregnancy. If freezing between ages 35 and 38, the rate goes down to 35%. If
freezing at 39 or 40, it's 20 to 25%, and if freezing eggs over age 40, the
success rate will be less than 10%. |
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l*****9
发帖数: 9501 | 4 http://www.businessweek.com/news/2012-02-27/ovarian-stem-cells-
Ovarian Stem Cells Make Human Eggs in Possible Aid to Fertility
By Ryan Flinn
Feb. 27 (Bloomberg) -- Stem cells taken from human ovaries were used to
produce early-stage eggs by scientists in Boston who may have created a new
method to help infertile women.
Females have a fixed number of eggs from birth that are depleted by the time
of menopause. The finding, published today in the journal Nature Medicine,
challenges the belief that... 阅读全帖 |
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w***b
发帖数: 34 | 5 This post greatly exaggerated the risk of IVF.
1. The success rate is pretty good for women under 40 who are healthy (i.e.
can produce eggs). Per cycle success rate is about 27% for women 35-40. You
have more than 60% of chance of getting a live birth with 3 tries. The rate
does decrease dramatically after 40. If you want a baby with or without a
family, it would be a good idea to froze some eggs before 35.
2. The procedure itself is a pretty small operation and far from "very
painful". The ov... 阅读全帖 |
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H*******g
发帖数: 6997 | 6 ☆─────────────────────────────────────☆
laotaiye111 (和马桶对话无聊) 于 (Sat Sep 15 14:40:02 2012, 美东) 提到:
IVF发展到现在，的确帮不少不孕妇女解决生育问题，但目前而言存在非常多问题，
很多根本问题是不可能通过技术手段来解决，所以大龄女要骗萎缩男年龄大生娃没问题
，有IVF照着，是非常不道德的事情。
几个误区：
1）IVF看似神秘，其实很简单。 就是把女人的卵子取出，然后体外受精，最后重新植
入受孕。
需要注意的是，IVF不能给你造卵子，卵子都来自女方，所以女人年龄越大，卵子越少
质量越差。过35岁，卵子质量是快速下降。IVF技术在大龄病人群体的成功非常低，主
要就是这个卵子和胚胎质量问题。这个是没办法解决的问题。只有上帝才能帮35+大龄
女，或者就是纯粹好运气。
当然你可以说我不用自己的卵子，靠别人捐卵行不行？行，国外允许你自己找年轻的捐
卵者。国内是不允许，只能用其他病人剩下的。当然你自己手眼通天能找到医院私下做
算本事。另外，你愿意替别人生娃吗？你能想通了就好
2）IVF过程是一个... 阅读全帖 |
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B********e
发帖数: 19317 | 7 http://evolution.berkeley.edu/evolibrary/article/0_0_0/evo_23
Development
Development is the process through which an embryo becomes an adult organism
and eventually dies. Through development, an organism's genotype is
expressed as a phenotype, exposing genes to the action of natural selection.
Studies of development are important to evolutionary biology for several
reasons:
Explaining major evolutionary change
Changes in the genes controlling development can have major effects on the
morphology |
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B********e
发帖数: 19317 | 8 http://evolution.berkeley.edu/evolibrary/article/side_0_0/hoxgenes_01
"General purpose" control genes are important elements in building
complicated organisms like flies. Some "control" genes are common to many
organisms (they are homologous — inherited from our common ancestor). For
example, Hox genes help lay out the basic body forms of many animals,
including humans, flies, and worms. They set up the head-to-tail
organization. You can think of them as directing instructions as an embryo
devel |
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m****r
发帖数: 1904 | 9 “The expulsion of an embryo or fetus before it can live on its own. Common
use often distinguishes between abortion and miscarriage, the former being
defined as the deliberate and induced emptying of a pregnancy.”
“Unavoidable abortion or miscarriage may be caused by accident, infectious
disease, mental or physical stress and strain, or because of a general
organic weakness on the part of the mother.”
“Deliberately to induce abortion or miscarriage by artificial means,...... is
an act of high cr... 阅读全帖 |
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m****r
发帖数: 1904 | 10 “There was no people cloning when all the articles were printed, with the
exception of creating them for embryonic stem cells. So they were making a
baby to kill it for cells for someone else. I think it's very plain that
that would be murder. Also, one source said that there is a death rate of
more than 10 times the normal for embryos. There wasn't a What is the Bible
's View article, that I could find. Maybe they'll revisit the topic again..
.” |
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m****r
发帖数: 1904 | 11 " As to cloning, how did Jehovah set up human reproduction? If the Bible
doesn't say for sure, Yes or No, we can't, but we can take what he's done,
and principles, and reason on them. Stem cell medical treatment would
depend, at least in part, on the source of the stem cells. You can get them
from your own body (that's what produces red blood cells), or from embryos.
One is murder, one isn't..." |
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X***y
发帖数: 3947 | 12 谢谢，这到是和我前两天请教的一个bio研究生说法一致。不过，他说stem cell其实分
裂的过程中拿走一个俩的并不影响胎儿发育。所以murder严格说并不成立啊。
them
embryos. |
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wh
发帖数: 141625 | 13 有的有道理。不过“中国人从英文翻译成中文，经常不注意忠实於原著。而是任意加油
添酱，把翻译当成是创作”，这个不都是“缺乏敬业精神”，有的就是觉得意译更好。
尤其他举例的电影片名。魂断蓝桥、孤星血泪是好还是不好？风气也有变迁，早一代的
片名意译多，现在的直译多吧。
蒋经国那句，作者的解释不对吧。“did little good”不是“的确有了点好处”，是
几乎没什么好处。他批译文没有翻译后半句，可译文里明明有。
马克思的原文是宗教是人民的鸦片，前面一句是被压迫心灵的叹息，整体是反宗教的。
网上有篇潘岳的文章说“宗教是统治阶级麻醉人民的鸦片”是列宁提出来的，但wiki上
列宁关于宗教是鸦片的引文也没这个意思。混乱……马克思的原文：
... Religion is the general theory of this world, its encyclopaedic
compendium, its logic in popular form, its spiritual point d'honneur, its
enthusiasm, its moral sanction, its sole... 阅读全帖 |
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b********n
发帖数: 38600 | 14 http://www.nytimes.com/2012/03/25/opinion/sunday/bruni-a-cathol
March 24, 2012
Rethinking His Religion
By FRANK BRUNI
I MOVED into my freshman-year dorm at the University of North Carolina after
many of the other men on the hall. One had already begun decorating. I
spotted the poster above his desk right away. It showed a loaf of bread and
a chalice of red wine, with these words: “Jesus invites you to a banquet in
his honor.”
This man attended Catholic services every Sunday in a jacket and tie,
... 阅读全帖 |
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E*****m
发帖数: 25615 | 15 来自主题: TrustInJesus版 - 进化与进化 from the animal that we don't think it's chicken yet.
http://en.wikipedia.org/wiki/Chicken_or_the_egg
The modern chicken was believed to have descended from another closely
related species of birds, the red junglefowl, but recently discovered
genetic evidence suggests that the modern domestic chicken is a hybrid
descendant of both the red junglefowl and the grey junglefowl.[14] Assuming
the evidence bears out, a hybrid is a compelling scenario that the chicken
egg, based on the second definitio... 阅读全帖 |
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D*****r
发帖数: 6791 | 16 这篇半页的新闻报道，只是一个杂志编辑（并非科研人员）根据Richardson等人的发现
，做的一个有煽动性的报道，这非常正常。能让基督教的信徒去读科学杂志上的哪怕报
导的摘要也make me smile.
相关的后续澄清性质的文章很多，前面转的文章里就提到Richardson本人写的澄清，还有
比如科学史方面的人针对这篇报导写的：
Robert J. Richards (2009) "Haeckel's embryos: fraud not proven" Biology and
Philosophy 23:147-154.
钱锟和各种基督教网站，对着这点鸡毛事情大惊小怪，引申起来high得没边，好像进化
论就推翻了似的，真滑稽。
在人类文明科学历史过程里，这些都是渣渣。
like
proving |
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w*********r
发帖数: 2192 | 17 Fulfilling Your Dreams with the
Seven Spiritual Laws of Success
By Deepak Chopra, M.D.
Deepak Chopra is a bestselling author, educator, and holistic health pioneer
who shares his insights in this inspiring article on personal growth.
Read the Chopra Interview
Deepak Chopra photo
There are many aspects to success; material wealth is only one component
. Moreover, success is a journey, not a destination. Material abundance, in
all its expressions, happens to be one of those things that mak... 阅读全帖 |
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b*******g
发帖数: 1309 | 18 1. Chen JY, Bottjer DJ, Davidson EH, et al.
Phosphatized polar lobe-forming embryos from the Precambrian of Southwest
China
SCIENCE 312 (5780): 1644-1646 JUN 16 2006
第一作者南大，第一通讯单位南大
2. Qiu MH, Ren ZF, Che L, et al.
Observation of Feshbach resonances in the F+H-2 -> HF+H reaction
SCIENCE 311 (5766): 1440-1443 MAR 10 2006
第二通讯作者南大化学系的老谢，无数人受其谱学原理的折磨，据说第一通讯作者是他
的学生（没有求证）
3. Ji Q, Luo ZX, Yuan CX, et al.
A swimming mammaliaform from the Middle Jurassic and ecomorphological
diversification of earl |
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C******9
发帖数: 1 | 19 conception:
1. a. Formation of a viable zygote by the union of the male sperm and
female ovum; fertilization. b. The entity formed by the union of the male
sperm and female ovum; an embryo or zygote.
2. a. The ability to form or understand mental concepts and abstractions. b
. Something conceived in the mind; a concept, plan, design, idea, or
thought. See Synonyms at idea.
3. Archaic A beginning; a start.
他真的用了“conception”吗？ |
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s*****e
发帖数: 970 | 20 日前，干细胞研究领域的国际顶尖杂志Cell Stem Cell（2011年1月号）刊发了上
海交通大学医学院/中科院上海生科院健康科学研究所完成的题为《Calcineurin-NFAT
信号通路对小鼠胚胎干细胞及胚胎早期胚层分化的重要调控作用》（Calcineurin-NFAT
Signaling Critically Regulates Early Lineage Specification in Mouse Embryonic
Stem Cells and Embryos）的论文，该研究筛选到一条在胚胎干细胞（ES）分化过程
中起关键作用的信号通路——calcineurin-NFAT,并对其在ES细胞分化和小鼠胚胎早期
发育过程中的功能进行了深入研究，从而为深入理解ES细胞自我更新与分化，以及胚胎
早期发育的调控机制提供了新的思路，对于成体细胞的重编程过程也有重要的提示作用，
有助于更早、更好地促进临床疾病的细胞治疗。
据了解，Cell Stem Cell是著名的Cell杂志出版社旗下的子刊。杂志目前的影响因
子为：23.563，为国际干细胞研究学会（ISSCR）所属期刊，每月一刊... 阅读全帖 |
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a*******i
发帖数: 27 | 21 【 以下文字转载自 Biology 讨论区 】
发信人: SWJSJ (BC), 信区: Biology
标 题: 单开一贴，浙大范衡宇涉嫌更加严重的剽窃和学术不端！
发信站: BBS 未名空间站 (Thu Sep 17 23:38:04 2015, 美东)
靠，实在是看着不爽，凭啥清北撕逼就大家一致讨伐，浙大范衡宇更加恶劣的剽窃别人
成果却没人关注？下面转帖，是从张生家和谢灿那个帖子里看到的，其他几位ID不愿意
单开一贴，我实在看不下去，特开一贴。这个范衡宇据说还拿到了杰青，国内科研真是
尼玛无底线了啊。
如果有知道内情的，在此帖讨论吧，不然都被清北的刷屏了。当然，也许有其他隐情冤
枉了范衡宇，也可以内部人出来解释解释。如果确定是剽窃，这尼玛妥妥的应该上新语
丝头条啊。
以下转帖：
----------------------------------------------------------------------------
------------------------
发信人: forjoy (三分华盖), 信区: Biology
标 题: Re: 能拿炸药奖吗？
... 阅读全帖 |
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c**i
发帖数: 6973 | 22 【 以下文字转载自 shopping 讨论区 】
发信人: choi (choi), 信区: shopping
标 题: An Apple Prototype
发信站: BBS 未名空间站 (Sat Jul 10 13:17:29 2010, 美东)
admin, iWatch U !!! ? Apple.pro, July 9, 2010.
http://apple.pro/
My comment:
(a) Click the main article in the home page.
(b) 3CM means "3 centimeters."
(c) A watch is a mere figment of imagination.
(d) The web site is based in Taiwan, said to have a good track record of
unearthing Apple products while in embryo stage. This blog is translated
into various languages alrea |
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M****e
发帖数: 70 | 23 For detail, read Nature 419:334 (2002)
While a short time ago there was this depute about the credibility
of certain people in China who declared that he had obtained
progress toward final cloning of human organs, now the truth
is that virtually many scientist have started the work using
stem cells derived from human embryos, and more shocking is
that some of them have already put effort into clinical trials in China.
Most of them got training in the US and then established their
own labs with g |
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b*********t
发帖数: 877 | 24 在这里帮你解答算不算作弊啊，呵呵。
先声明我其实也不是专搞这个的。以前学了一点毛皮而已。你要是去看点early
embryonic dev.的大牛如Elizabeth Roberson 跟Bridget Hogan的文章就会知道是该怎么
答了。
答案一：cell non-autonomous。 这就是说这个yfmg either 是个secreted factor
itself, or is upstream in a pathway that results in the secretion of other
important factors.
但是要test this hypothesis through chimera expt好象不容易。我还没想出来。
答案二：这个yfmg is involved in the development of the trophoblasts
, which do NOT contribute to the actual embryo proper. 你的ES cells
injected get integrated into the inn |
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M*****n
发帖数: 16729 | 25 MCBJC Discussion for the week of 4/21
Sonic Hedgehog: A Morphogen and Axonal Guidance Cue
Hedgehog is probably the most well-known morphogen in embryo development. As a
multifunctional molecule involved in cell differentiation, cell proliferation
and tissue patterning, hedgehog's functions as a morphogen have been
extensively studied. However, recent studies about another morphogen - BMP
family revealed its involvement in repelling spinal cord commissural axons.
Thus the possibility that Sonic H |
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r****o
发帖数: 105 | 26 本文献给YL
（二）梦之队
六月六号晚上，讲课老师和学生在冷泉港Beckman Neuroscience Building 的
Plimpton conference room会面，主要是相互认识，同时介绍这门课的光荣历史
和计划安排。冷泉港所开课程中历史第一悠久的是《高级细菌遗传学》，有五十
多年的历史，而第二悠久的就是《小鼠分子胚胎学》了。八十年代初的时候，转
基因老鼠技术刚刚诞生不久，Watson提议开一门课程教人做显微注射。于是
１９８３年开了第一次课，一直延续到现在，到今年已经是２４届。在二十多年
的时间里面，授课内容从单一的显微注射拓宽到小鼠胚胎发育的各种实验技术。
这门课实验部分覆盖面之广是我上课之前完全没有意识到的。有多广呢？大家可
能都熟悉一本厚厚的实验手册叫做“Manipulating the Mouse Embryo:a
laboratory manual”。这本手册描述的实验８０％我们都做过或者演示过。而且
最难最有用的实验技术我们必须自己动手做。按主讲老师Michael Shen的说法就
是，这些技术是dying technique。因为很多小鼠胚胎发育 |
|
r****o
发帖数: 105 | 27 本文献给YL
（五）shock and awe
上一节里，我提到了六月七号第一天所涉及的动物实验基本功。有网友可能因此觉得
这个课教的东西很简单。要这么想就大错特错了，这个课程的特点是前难后易，第一天的
实验就很麻烦。一下午实验下来，我好像脱了一层皮，整个感受可以用shock and awe来
形容。想到还要这样再熬２０天，我就直哆嗦。当时甚至有了要求退钱走人的想法。当然
了，我最后还是坚持住了，十天之后重温第一天实验的时候，突然觉得其实一点也不难。
当然这是后话了。
为了让大家理解第一天实验的重要性，我先简单介绍一下小鼠的着床前胚胎发育（pre
-implantation embryo development）。雌鼠和雄鼠交配之后，从卵巢排出的卵子在输卵
管壶腹部（ampula）受精。大概在1.5dpc（days post coitum，交配后天数）的时候，受
精卵开始分裂成双细胞胚胎，并在一天左右的时间里分裂成八细胞胚胎。在这个分裂过程
里面，细胞虽然进行分裂，但是并没有生长，所以整个胚胎的体积没有太大变化。从八细
胞胚胎开始，分裂的细胞开始表达一些细胞粘连分子，以至于所有细 |
|
r****o
发帖数: 105 | 28 本文献给YL
（八）Matrix Reloaded
六月七号第一天实验中最重要的一个项目是胚胎子宫转移（uterine transfer）。
这个实验的目的在于把经过体外培养和修饰过的囊胚（blastocyst）放回到假孕小鼠的子
宫里，使之着床（implantation）并且正常发育。没有这个技术存在，基因敲除小鼠是绝
对做不出来的。前面提到过，Anne Mclaren一手发展了这个技术，奠定了小鼠胚胎研究的
基础。原始的文章发表在Nature上，有兴趣的同学可以查阅一下：McLaren A. and
Biggers J.D. 1958. Successful development and birth of mice cultivated in
vitro as early embryos. Nature 182:877-878。
把囊胚（blastocyst）放入子宫（uterus）本身并不能保证囊胚的正常发育，只有
当囊胚正确的着床（implantation）之后才能进一步发育。而顺利的着床（implantation
）需要怀孕雌鼠在特定时间“窗口”大量分泌一些激素。由于这种激 |
|
r****o
发帖数: 105 | 29 本文献给YL
（十）Richard Behringer的练习题
上一篇提到了小鼠的毛色。Blanche在讲课过程专门出了七道关于毛色的练习题
让我们思考。这七道题目是由MD Anderson的Richard Behringer准备的，这老哥精通小鼠
遗传学，是 “manipulating mouse embryo: a laboratory maual”的作者之一。由于实
在没有任何这方面的基础，在讨论这几个题目的时候，我完全是糊里糊涂的。现在上完课
之后，重新研习了一下毛色的基本知识，再来看这些题目，发现一点都不困难。下面就是
这些题目。
（１）albino的雌鼠和albino的雄鼠交配。子代的毛色是什么？C位点的基因型
是什么？
（２）albino的雌鼠和黑色雄鼠交配，子代全是agouti。请问亲代的A位点基因
型是什么？
（３）黑色雌鼠和褐色雄鼠交配。部分子代是冲淡的黑色和冲淡的褐色。请问亲
代的D位点基因型是什么？
（４）一只黑色的雌鼠和一只albino的雄鼠交配。所有的F1子代都是agouti。F1
子代相互交配后，F2子代有五种毛色表型, 包括agouti，albino，黑 |
|
r****o
发帖数: 105 | 30 本文献给YL
（十四）不归之路
介绍完六月七号第一天上午的课程之后，现在我来讲讲晚上由Patrick Tam讲的
Introduction to mouse development。由于对发育知之甚少，这个讲座让我如坠五里
雾。说来惭愧，过去买过Gilbert编的Developmental Biology，但始终没读进去。这本
书虽然很权威，但是它的编排顺序却很奇怪，比如把几个脊椎动物的发育混在一起讲，
让人感觉很糊涂。Manipulating the Mouse Embryo: a laboratory manual的第二章专
门介绍小鼠的发育，非常之全面，就是挺复杂，初学者不容易理清楚脉络。从这一篇开
始，我将介绍小鼠胚胎发育的最基本过程和一些专有名词。
哺乳动物遗传学是从上个世纪初小鼠的研究开始的，但哺乳动物的胚胎发育学却
是从研究兔子开始的，时间也早了二三十年。现在我们常用的这些小鼠胚胎发育的技术
多是受了兔子胚胎发育研究的启发。上个世纪初小鼠作为实验模型之后，人们对小鼠生
殖方面的了解越来越多，所以哺乳动物胚胎发育研究逐渐转为用小鼠为模型。用小鼠来
研究胚 |
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t*x
发帖数: 1065 | 31 I do mid-brain cultures from mouse embryos. So far, my cells are not
consistent. Sometimes they spread out individually (great), but sometimes
they clump up as clusters. It looks like a coating problem. I coated with
poly-D-lysine at 0.1mg/ml overnight and washed with water twice and then
10ug/ml laminin coating overnight. After that, wash by water twice and dried.
Anyone has suggestion about a coating technique that is consistent with cell
spreading (not clumping)?
Thanks a lot! |
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z****u
发帖数: 1007 | 32 一直就是用的 Superfrost plus的slide切的。。。
我现在protocol是切完后55度考O/N然后再开始IHC 或者ISH.
这样比不烤好很多，如果是embryo sample那也没问题，能经受住操作。
但是我的samle是adult mice incisor ,经过20天decalcification的。
即使这样仍然很容易脱片。
尚且还未尝试过polylysin coatin. 没和superfrost plus比较过。
蛋青甘油是啥？ 英文是什么？ |
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w****t
发帖数: 137 | 33 哪个牌子的Dissecting Microscope比较好？大家有什么model可以推荐吗？要可以方便
解剖mouse embryo 6.5, 7.5天 的，可以加配相机的。谢谢！ |
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s******y
发帖数: 28562 | 34 We use Jenco (USA). It come with a digital camera adapter.
We have been using it for our mouse embryo manipulation for several years.
Pretty convenient for our work, and very very affordable. |
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w****t
发帖数: 137 | 35 Thanks a lot! Could you please provide a model number for me? Is it good for
dissect small embryos (E6.5?) |
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s******y
发帖数: 28562 | 36 GL7-290. We ordered it through ISC Bio-express. I think you can check the
price through their website. http://www.bioexpress.com/
E6.5 embryo? That would be very, very small. I am sure you can see it under
that microscope, but you will have to have very good hands to handle it.
We only deal with stuffs older than E12.
for |
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b******l
发帖数: 1632 | 37 Curr Top Dev Biol. 2008;82:1-22.
Ontogeny of erythropoiesis in the mammalian embryo.
McGrath K, Palis J.
五个学校的 e-journal 都找遍了，竟然都没这期刊。
哪位恰好能 access，那是缘分哪，就麻烦发到 g*********[email protected] 吧。
多谢了，多谢了。 |
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s******y
发帖数: 28562 | 39 If you are talking about the maximum time?
For whole embryo, sometimes we keep them in 100% ethanol for a long long
time like several month. But for slides I don't know, because we never keep
them for more than 24hours so I cannot answer that. |
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d******r
发帖数: 124 | 40 最近在培养neuron，今天是第四天，发现很多细胞聚集在一起，中间的看起来分化得也
不好，neurite很短，如图所示。请高人鉴定一下，这些是不是neuron？可能是什么原
因造成的？我是从E18 mouse embryo分离的neuron，用波片倒扣在astrocyte
monolayer上培养。medium用的是neurobasal+B27。谢谢！ |
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r**a
发帖数: 121 | 41 请问做果蝇的人
知道这个driver有在第二个染色体上的吗？
我的那个基因，可能是每个细胞都必须要的基因，在第三个染色体上面
我用actin-gal4来rescue，发现还是不行
查了之后，发现这个actin-gal4在embryo不是在所有细胞表达（scattered expression）
所以想用tub-Gal4，可是这个基因在第三个染色体上，这样不能用来做rescue
请问，有人知道有在第二个染色体上的tub-Gal4吗？
或者有其他什么办法（难不成把tub-Gal4重组到我的突变体上）？
谢谢了！ |
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s******s
发帖数: 13035 | 42 比较敏感就用hood啦。像我不敏感的收集1hr embryo的时候
一天做个七八次也没觉得啥。我老板一个朴刀同事当年对果蝇
过敏，最后被迫转行
heptane |
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S*********e
发帖数: 127 | 43 Lymphatic development.
Butler MG, Isogai S, Weinstein BM.
Birth Defects Res C Embryo Today. 2009 Sep;87(3):222-31.
a*****[email protected]
Thanks a lot. |
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c**a
发帖数: 94 | 44 需要从embryonic mice brain 里分离并培养 endothelial cells. 求protocol.
会不会embryo脑子里血管不够多,不容易培养? |
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j*****d
发帖数: 787 | 45 excel (for breeding and genotyping) + a 7x10 tables (for each cage)
go to mouse room every single day as I need embryos
I manage 7x5x10=350 clonies, not a huge number. |
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S*********e
发帖数: 127 | 46 Biochem Cell Biol. 1999;77(6):527-42.
Cell adhesion and the actin cytoskeleton of the enveloping layer in the
zebrafish embryo during epiboly.
Zalik SE, Lewandowski E, Kam Z, Geiger B.
a*****[email protected]
thanks alot |
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s******s
发帖数: 13035 | 47 1.首先你要保证你大瓶摇出来的菌和小摇出来的有一样多的质粒，
用大摇的菌取出来小抽一下看看？
2.不放心iso-pro沉淀的时候－70放一个小时先
3.强烈建议用50ml falcon tube做收集离心，干净，pellet清楚
注意速度不要超过6k，可以时间延长一些
Qiagen的Midi简直是最好的plasmid kit, 质粒绝对干净。以前实验
室做transgenic fly, 只有这个做出来的质粒打embryo不会有很高的
致死率 |
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S*********e
发帖数: 127 | 48 Hoch JR, Stark VK, van Rooijen N, Kim JL, Nutt MP, Warner TF. 1999.
Macrophage depletion alters vein graft intimal hyperplasia. Surgery 126:428
–37
Kennedy TE, Serafini T, de la Torre JR, Tessier-Lavigne M. 1994. Netrins are
diffusible chemotropic factors for commissural axons in the embryonic
spinal cord. Cell 78:425–35
Kolodkin AL, Matthes DJ, O’Connor TP, PatelNH, Admon A, et al. 1992.
Fasciclin IV: sequence, expression, and function during growth cone guidance
in the grasshopper embryo. Neur |
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