f*c
发帖数: 2726 | 1 以前我做transfection, fugene 6就用一般的DEME without serum dilute,
lipofectamine 2000才用OPTI-MEM，今天搞错了，用了OPTI-MEM reduced serum
dilute 去dilute fugene 6 (293T), 不知效果会怎样？有用过得吗？谢谢 |
|
g*******8
发帖数: 6 | 2 老板让我学做 RealTime pcr去quantify some sample dna。 但我做了好几次，
standard 四个点总不能连成很好一条线。我现在非常frustrated. 不知是standard 做
的不好还是加sample前mix的不好。无论怎样我猜都是mix的问题。我是这样做的： add
3ul standard into 27ul H2O to make 10 fold dilution 。 then pipett up and
down for 20 times to mix it and use it to make another 10 fold dilution. I
am not sure if I mixed very well before I make another 10 fold dilution.
Is vetex better? I am afraid vetex will break dna. So I tried not to use
vetex. If I can use vetex, which setting I shoul |
|
w********h
发帖数: 12367 | 3 dilute regime: C
entangled regime: C>Ce
both are almost clear in theory.
between C* and Ce, it is called semi-dilute.
de Gennes gave some prediction for this regime.
and many questions are undone in this regime.
hehe...later you will see my another paper on this subject,
although our interests are in dilute regime and entangled regime.
if you want to know further,
refer to de Gennes' books, or the new book of Rubinstein and Colby's.
a
but
chain. |
|
c*s
发帖数: 2145 | 4 发信人: zzwh (头碰桌), 信区: Macromolecules
标 题: 怎么判断溶液是semidilute or dilute?
发信站: The unknown SPACE (Sun May 18 13:21:35 2003) WWW-POST
thanks!
发信人: wonderlich (左岸，遁去), 信区: Macromolecules
标 题: Re: 怎么判断溶液是semidilute or dilute?
发信站: The unknown SPACE (Sun May 18 13:24:38 2003) WWW-POST
ft...overlapping concentration..
(C*)*[eta]=1---->(C*)
发信人: zzwh (头碰桌), 信区: Macromolecules
标 题: Re: 怎么判断溶液是semidilute or dilute?
发信站: The unknown SPACE (Sun May 18 14:22:43 2003) WWW-POST
eta is the screening length?
if I |
|
B**********r
发帖数: 7517 | 5 账面上没有这个填洞的说法。所以你没有事实，只有猜测。
总股数已经算上dilution了，你又额外来个70%的dilution，又是主观判断。 |
|
t****n
发帖数: 324 | 6 “For cesium and iodine, they are soluble,” Buesseler said in a telephone interview. “This time of year off the coast of Japan, they would mix with water down 100 feet to 300 feet, and be diluted by a factor of about 100. The currents there would move it to the south, just north of Tokyo, and then out to sea.”
http://www.bloomberg.com/news/2011-03-23/ocean-currents-natural
dilution factor 居然只有1/100。比我悲观设想的还差。这里谁有一手资料能推翻这个说法，宽慰众生？ |
|
R******d
发帖数: 5739 | 7
interview. “This time of year off the coast of Japan, they would mix with
water down 100 feet to 300 feet, and be diluted by a factor of about 100.
The currents there would move it to the south, just north of Tokyo, and then
out to sea.”
这个说法，宽慰众生？
this dilution factor is probably refering to 100 to 300 ft, not into the sea
. |
|
T**********t
发帖数: 1604 | 8 Vortex will not break DNA, don't be paranoid... You can vortex at full speed
and your DNA sample still stays intact.
Also, make sure you have a very accurate and calibrated pipette to use, in
this kind of series dilution, pipetting error could be detrimental to your
result. If your pipette is not accurate enough, try increase the volume of
each dilution so that you could reduce the effect of pipetting error. |
|
m******5
发帖数: 1383 | 9 sonication的设置被人改过没注意到，在dilution buffer（也就是immune
precipitation 反应的buffer）里取了一部分跑电泳，发现genomic带很清晰，smear分
子量都很大，并且很黯淡，这种情况下能在dilution buffer里紧急大功率补sonicate
一下么？非常感谢！！！！ |
|
w********h
发帖数: 12367 | 10 [eta] is intrinsic viscosity.
you can get criteria for dilute solution from any polymer textbooks..
overlapping concentration C* can be also deduced using a simple fomula.
but this predicted one generally is lower than the real overlapping
concentration.
what's your polymer?
people already have MHS formula for different polymers in different solvents.
you can first use MHS to get [eta], then C*.
what's dilute solution?
just means the regime in which eta-eta(s) is linear with C. |
|
w********h
发帖数: 12367 | 11 then you can measure the viscosity to see which is the critical concentration
for the transition from dilute to semi-dilute regimes... |
|
T**********e
发帖数: 29576 | 12 Outrage Dilution
Posted January 26th, 2017 @ 8:24am in SCOTT ADAMS' BLOG
I’m having a fun time watching President Trump flood the news cycle with so
many stories and outrages that no one can keep up. Here’s how the math of
persuasion works in this situation:
1 outrage out of 3 headlines in a week: Bad Persuasion
25 outrages out of 25 headlines in a week: Excellent Persuasion
At the moment there are so many outrages, executive orders, protests, and
controversies that none of them can get enough o... 阅读全帖 |
|
w**j
发帖数: 1032 | 13 Bank of America will let the courts sort the mortgage matters out
Bank of America Corp. (NYSE:BAC), the US’ largest lender by assets, said it
can meet capital goals without selling common stock and said it had
expected some of the recent “Noise” in mortgage-related legal actions.
“There are many non-dilutive ways for us to get there,” Chief Financial
Officer Bruce Thompson said Friday, referring to the firm’s regulatory
capital standards. Mr. Thompson commented in an internal memo to employees
o... 阅读全帖 |
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m*****i
发帖数: 4342 | 14 What a joke!
What about 700 million shares warrent to buffet at $7/share. No different
then a dilution.
it
because |
|
c*******9
发帖数: 6411 | 15 if somebody buy 20% stake, what happen? new shares issue for them, and
dilute the share holders? what is going on? |
|
c*******o
发帖数: 3829 | 16 I don't think so. BAC: diluted EPS 3 cents, adjusted EPS 31 cents. |
|
i*u
发帖数: 299 | 17 EPS Adjusted = Diluted EPS + Various One-Time Items
now i know what you are talking about. yes, you do want to consider "
everything". |
|
l******2
发帖数: 5522 | 18 这笔帐这么算：
每股$10增发股票， 就算一共100M股， 一共能筹$1B的现金。 加上现在$2B的equity,
一共$3B帐面价值。
增发后，$3B帐面价值 / 320M股票 = 每股$9.3的book value, 这和现在每股$10.3的
book valve很接近。
但是将来的每股盈利将会被dilute， 但是如果你在这个价位买JCP还是非常划算。 |
|
M********i
发帖数: 4082 | 19 这样算有个问题。筹来的$1B是来填洞的。这个洞有多大不知道，假设是$500M.
$2.5B/320M = $7.8
再狠点儿，算上dilute, $7.8 x 70% = $5.5 |
|
M********i
发帖数: 4082 | 20 哦 增发就是为了填洞的 我昨天扫了一眼 好像有看到 总之这个钱是要花出去的
谢谢提醒，dilution我算得不对。
Anyway,jcp的底在哪里？增发价打个7-8折 $6.6-$7.6 |
|
R******d
发帖数: 5739 | 21 as far as the data shown, it did dilute it to the minimum, didn't it? is
the risk for your health higher? yes, but should it be enough to affect your
daily function? I don't think so, although you have your freedom to do
whatever you want. the people who should concern the most is the japanese
people, they live right next to it. |
|
h****6
发帖数: 229 | 22 should be fine with your dilution buffer. |
|
m**********d
发帖数: 137 | 23 我在ChIP RNA Pol II, 然后qPCR定量某基因promoter region及gene body的 RNA Pol
II loading,以判断此基因转录是否在某些treatment下被激活
我试了abcam的 RNA Pol II antibody (ab817),效果还挺好的，我每个reaction（10cm
plate,80%confluent）都加10microlitter Ab,想问问版上常做这个assay的前辈，大
家都用什么样的antibody dilution，像我这么做着实太费抗体了 |
|
c*****e
发帖数: 238 | 24 I never do experiments, so I'm not sure.
Theoretically, the scattering function for a dilute solution scales like
exp(-N^2k^2/6), in a semidilute solution, it scales as exp(-\psi^2k^2/6)
so there should be a crossover in the plot of S(k) vs. k |
|
d*****w
发帖数: 124 | 25 Keep in mind C* is not a sharp but wide concentration regime. It gives a good
criteria from the estimation of isolated chain size base on the Kuhn length of
polymers. Only far below or above the concentration range you can say the
colution is dilute or semidilute.
fomula. |
|
w********h
发帖数: 12367 | 26 how far?
in fact, semi-dilute regime is really narrow. ~10% to C*.
and the critical concentration (empirical C*) is very clear (if not as sharp
as you expect) from the viscosity increment.
good
of
the
textbooks.. |
|
w********h
发帖数: 12367 | 27 molecular weight of your PS-PIP?
if higher than 100K, for sure in semi-dilute.
You can refer to Fetters' review article (Macromolecules 1994, 27:4642) to see
the /M values for PS and PIP.
0.675 for PIP, 0.434 for PS.
Suppose they are the same, say, 0.5.
It is almost half of PBD I am working on.
so, you can predict the overlapping concentration for PS-PIP is about 2.5
times that for PBD for the same Molecular weight.
From Colby's paper for PBD, (Macromolecules 1991, 24, 3873)
0.25 wt.% in g |
|
w********h
发帖数: 12367 | 28 approximately 58K PS,
then 5 wt% is still in dilute regime considering PBD58K's C* should be higher
than 3 wt%. according to my experience.
//BTW: Why PS-PEO is so interesting?
So many people are working on that ;-)
a |
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d*****w
发帖数: 124 | 29 Oh, no. Actually for current polymers, i.e. N=1000-10000, the C* is about
10^-3,
and the semi-dilute regime crosses over about two decades. So most interests
of polymer solutions focus on this regime. Just my 2 cents.
length
not.
overlapping
different |
|
w********h
发帖数: 12367 | 30 C*---M^(-0.5 or -0.6)
how can u say "N=1000-10000, the C* is about 10^-3"?
semi-dilute regime is from C* to ~10%.
refer to Colby's paper I mentioned.
And you said "most interests of polymer solutions focus on this regime",
but I think the theory in this regime is mostly undone.
de Gennes proposed something in 1971, Macromolecules,
but still not good enough.....
sharp
the
but
chain. |
|
a***n
发帖数: 578 | 31 I am curious what is the difference between semidilute and dilute? is there a
reason to seperate them? thanks.
not. |
|
d****o
发帖数: 44 | 32 Rheological Properties. There must be entanglements in semidilated solutions
but not in diluted one. |
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w********h
发帖数: 12367 | 33 sorry, in semi-dilute regime, no entanglements. |
|
j****i
发帖数: 742 | 34 I bought WUBA back today and bought it a lot because it is so cheap...
-----------------------------------------
——$58同城(WUBA)$ 总营收为6460万美元，同比增长83.9%，高于6100万至6300万美元
的公司指导性预测。
——毛利率为94.7%，高于去年同期的93.9%。
——Q2销售及市场费用4030万美元，同比增106.6%，其中广告花费高达1650万美元[心
碎了]；
——non-GAAP(非美国通用会计准则)净利润为1240万美元，去年同期为550万美元。
——截至6月30日，公司现金、现金等价物、短投共计4.143亿美元；Q2运营现金流为
2220万美元；
——公司预计第三季度总营收6600-6800万美元，增速在59-63%。
Second Quarter 2014 Financial Highlights
Total revenues were US$64.6 million, an 83.9% increase from the same p... 阅读全帖 |
|
y*****l
发帖数: 5997 | 35 来自主题: _pennystock版 - 半导体残了 盘后不是大涨了吗？还用分析啥？
我不会看报表，让红哥来吧。
看起来很不错，图也很牛。
SIMO你推荐了很多次都没买，可惜了。
神马都是浮云！
http://finance.yahoo.com/news/Silicon-Motion-Announces-pz-34337
Second Quarter 2011
Financial Highlights
Net sales increased 16% quarter-over-quarter to US$50.5 million from US$
43.4 million in 1Q11
Gross margin excluding stock-based compensation increased to 46.9% from
46.2% in 1Q11
Operating expenses excluding stock-based compensation, acquisition-
related charges, and other items decreased to US$13.1 million fr... 阅读全帖 |
|
r*****e
发帖数: 7853 | 36 SUGAR LAND, Texas, Nov. 07, 2017 (GLOBE NEWSWIRE) -- Applied Optoelectronics
, Inc. (AAOI), a leading provider of fiber-optic access network products for
the internet datacenter, cable broadband, fiber-to-the-home (FTTH) and
telecom markets, today announced financial results for its third quarter
ended Sept. 30, 2017.
“While our third quarter results were negatively impacted by lower demand
from a large customer, we continued to experience solid demand from our
other large datacenter customers, ... 阅读全帖 |
|
|
C*I
发帖数: 4736 | 38 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 39 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 40 Published: 30 October 2013
Isolation and characterization of a bat SARS-like coronavirus that uses the
ACE2 receptor
Xing-Yi Ge, Jia-Lu Li, Xing-Lou Yang, Aleksei A. Chmura, Guangjian Zhu,
Jonathan H. Epstein, Jonna K. Mazet, Ben Hu, Wei Zhang, Cheng Peng, Yu-Ji
Zhang, Chu-Ming Luo, Bing Tan, Ning Wang, Yan Zhu, Gary Crameri, Shu-Yi
Zhang, Lin-Fa Wang, Peter Daszak & Zheng-Li Shi
Nature volume 503, pages535–538(2013)Cite this article
Abstract
The 2002–3 pandemic caused by severe acute respirator... 阅读全帖 |
|
t******t
发帖数: 121 | 41 Baidu Announces Fourth Quarter and Fiscal Year 2010 Results 01/31 04:
30 PM
BEIJING, Jan. 31, 2011 /PRNewswire-Asia/ -- Baidu, Inc. (BIDU:$108.63,00$2.
09,001.96%) , the leading Chinese language Internet search provider, today
announced its unaudited financial results for the fourth quarter and fiscal
year ended December 31, 2010(1).
(Logo: http://photos.prnewswire.com/prnh/20081103/BAIDULOGO)
Fourth Quarter and Fiscal Year 2010 Highlights
Total revenues in the fourth quarter of 2010 were ... 阅读全帖 |
|
z*********1
发帖数: 192 | 42 Gilead Sciences 3Q Rev $6.04B >GILDBY Dow Jones & Company, Inc.
— 4:05 PM ET 10/28/2014
Gilead Sciences Announces Third Quarter 2014 Financial Results
- Product Sales of $5.97 billion -
- Sovaldi Sales of $2.80 billion -
- Non-GAAP EPS of $1.84 per share -
- Revised 2014 Full Year Guidance - FOSTER CITY, Calif.--(BUSINESS WIRE)--
October 28, 2014--
Gilead Sciences, Inc. (GILD
Loading...
) announced today its results of operations for the quarter ended September
30, 2014. Total re... 阅读全帖 |
|
d******8
发帖数: 1972 | 43 SUNNYVALE, Calif.--(BUSINESS WIRE)-- Yahoo! Inc. (NASDAQ:YHOO - News) today
reported results for the quarter ended September 30, 2011.
Revenue excluding traffic acquisition costs (“revenue ex-TAC”) was $1,072
million for the third quarter of 2011, a 5 percent decrease from the third
quarter of 2010. Income from operations decreased 6 percent to $177 million
in the third quarter of 2011, compared to $189 million in the third quarter
of 2010. The year over year decreases were primarily due to the ... 阅读全帖 |
|
g*********d
发帖数: 233 | 44 交大招的法国Nobel奖原来是去搞伪科学的
http://www.btsmth.com/show_snapshot.php?en_name=TsinghuaCent&gid=456861
Science诺奖得主吕克·蒙塔尼访谈
French Nobelist Escapes "Intellectual Terror" to Pursue Radical Ideas in
China
Martin Enserink
PARIS—Virologist and Nobel laureate Luc Montagnier announced earlier
this month that, at age 78, he will take on the leadership of a new
research institute at Jiaotong University in Shanghai. What has shocked
many scientists, however, isn't Montagnier's departure from France but
what he pla... 阅读全帖 |
|
d****7
发帖数: 2241 | 45 【 以下文字转载自 PraticalTrading 俱乐部 】
发信人: googer (cutie), 信区: PraticalTrading
标 题: 10 Bank Stocks Trading Below Book Value
发信站: BBS 未名空间站 (Fri Jun 18 16:41:23 2010, 美东)
这篇文章对几个小银行做了一个比较好的介绍，包括 fbp, crbc, pcbc, bbx, prwt,
snv, bpop, cse, mi & c. 每个包括目前状态，资本充足与否，可能风险，资产品质以及可
能前景。 可做参考。
10 Bank Stocks Trading Below Book Value
FBP
First Bancorp (FBP) of San Juan, Puerto Rico was trading for just 0.2 times
tangible book value on Thursday, when shares closed at $1.06, down 54% year-
to-date.
TARP Status: ... 阅读全帖 |
|
s*******t
发帖数: 335 | 46 Financing Options for Small Biotechnology Companies
If you are an investor in biotechnology companies, periodic financings are a
fact of life and necessary for the company to survive and be successful.
Nevertheless, there is a belief among some investors that anytime a Company
raises money through issuing shares, it dilutes and harms existing
shareholders. However, issuing new shares is not always a bad thing for
existing shareholders. The willingness of new investors to put money into a
company... 阅读全帖 |
|
m******u
发帖数: 12400 | 47 NEW YORK, Aug. 7, 2013 /PRNewswire/ -- Eagle Bulk Shipping Inc. (Nasdaq:
EGLE) today announced its results for the second quarter ended June 30, 2013
.
For the Second Quarter:
Net reported loss of $3.0 million or $0.18 per share (based on a
weighted average of 16,968,750 diluted shares outstanding for the quarter),
compared with net loss of $23.1 million, or $1.46 per share, for the
comparable quarter of 2012.
Net revenues of $44.2 million, compared to $48.5 million for the
comparable qu... 阅读全帖 |
|
b***e
发帖数: 1419 | 48 我的意思就是说吧，你不能老用Bill Gates, Steve Jobs这种的来举例子。最后大家搞
的都和小姑娘信韩剧一样，觉得要不是两个以上的高富帅倒追都不好意思跟人打招呼。
我只是跟你说普遍的情况而已。
就说你这俩例子吧。 第一个其实范围内。大家现在一个误解就是一个startup不弄个1b
最后不好意思跟人打招呼。这个完全是韩剧流。最后上b的startup有几个？ 你两只手
放在一起，基本能数过来。其实一个startup能卖个100M-200M就算成功，能到300M-
500M就算是肥肠的成功了。上b被收购的近年来也就是youtube和instagram了。Zynga都
火成什么样了，也不过是2b而已。你这个150M的例子你就说能上b? Statistically,
我不信。如果最后是300M的话(这个已经算客观上乐观的估计了)，这些股(在不考虑
dilution的情况下)也就是30W而已。那个couter offer的也不是普遍情况。不可能每个
人要走都是0.2%的counter。而且这种information绝对不外传，因为会引起team
instability。大家全来要c... 阅读全帖 |
|
g*********5
发帖数: 2533 | 49 Isolation of nuclei for the co-IP and whole ChIP protocols is based on the
methods of Gendrel et al. (2002, 2005), Johnson et al. (2002), and Nelson et
al. (2006).
METHOD
For extraction and co-IP of nuclear proteins, see Steps 1-39 (Fig. 1). For
the ChIP procedure, see Steps 40-69 (Fig. 2).
Figure 1.
View larger version:
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Figure 1.
Flowchart for the timeline and organization for co-IP of nuclear proteins
from Arabidopsis seedlings.
Figure 2.
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发帖数: 812 | 50 This is the express transcript from Factset.
Operator: Good morning and welcome to the First Quarter 2011 Western
Refining Earnings Conference Call. After the speakers' opening remarks,
there will be a question-and-answer period. [Operator Instructions] As a
reminder, ladies and gentlemen, this conference call is being recorded and
your participation implies consent to our recording of this call. If you do
not agree with these terms, please disconnect at this time. Thank you.
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