j****0
发帖数: 1074 | 1 depends where they are going to get the money to build the giga factory.
if no dilution, 280.
if dilution 220
:-) |
|
s***i
发帖数: 603 | 2 盘后爆了，但怎么解读这个ER？
Net income in the fourth quarter of fiscal 2013 was $2.0 million, compared
to $20.6 million in the corresponding period in fiscal 2012. Diluted
earnings per share in the fourth quarter of fiscal 2013 were $0.04, compared
to diluted earnings per share of $0.43 for the corresponding prior year
period |
|
c******n
发帖数: 65 | 3 Total revenues in the second quarter of 2014 were RMB11.986 billion ($1.932
billion), a 58.5% increase from the corresponding period in 2013.
Operating profit in the second quarter of 2014 was RMB3.558 billion ($573.6
million), a 22.5% increase from the corresponding period in 2013.
Net income attributable to Baidu in the second quarter of 2014 was RMB3.547
billion ($571.7 million), a 34.1% increase from the corresponding period in
2013. Diluted earnings attributable to Baidu per ADS for the sec... 阅读全帖 |
|
d******8
发帖数: 2191 | 4 Net income for the second quarter of 2014 was $3.8 million, or $0.02 per
share on a basic and diluted basis. Included in the net income for the
second quarter of 2014 was a gain related to the change in fair value of
previously issued common stock warrants of $9.6 million. Excluding this item
, adjusted net loss for the second quarter of 2014 was $5.8 million, or $0.
04 per share on a basic and diluted basis.
Plug Power had cash and cash equivalents of $168.6 million and net working
capital of $... 阅读全帖 |
|
k********6
发帖数: 175 | 5 last column is Q3 2014
GAAP net income/(loss) per share - basic $ (0.08 ) $ (0.04 ) $ (0.11
) $ (0.05 ) $ 0.00 $ (0.04 ) $ 0.11
GAAP net income/(loss) per share - diluted $ (0.08 ) $ (0.04 ) $ (0.
11 ) $ (0.05 ) $ 0.00 $ (0.04 ) $ 0.10
Non-GAAP net income/(loss) per share - basic $ (0.03 ) $ (0.05 ) $ (
0.07 ) $ 0.07 $ 0.07 $ (0.02 ) $ 0.18
Non-GAAP net income/(loss) per share - diluted $ (0.03 )
$ (0.05 ) $ (0.07 ) ... 阅读全帖 |
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N****p
发帖数: 1691 | 6 "The second quarter 2015 market environment remained extremely challenging
for our steel and metals recycling operations," said Mark D. Millett, Chief
Executive Officer. "The ongoing flood of steel imports continued to pressure
steel product pricing to a greater degree than the benefit realized from
lower scrap costs, compressing second quarter steel margins. However, due
to continued solid U.S. steel demand, our second quarter steel shipments
improved, which offset most of the margin compressi... 阅读全帖 |
|
h****a
发帖数: 2095 | 7 CHICAGO, July 23, 2015 /PRNewswire/ --United Airlines (UAL) today
reported second-quarter 2015 net income of $1.3 billion, or $3.31 per
diluted share, excluding $67 million of special items. Including special
items, UAL reported second-quarter net income of $1.2 billion, or $3.14 per
diluted share. These results are a record quarterly profit for the company.
The company's Board of Directors authorized an additional $3 billion share
repurchase program, which the company expects to complete by the... 阅读全帖 |
|
c*****r
发帖数: 529 | 8 http://truthcdm.com/this-could-be-the-end-for-big-oil/
This Could be the End for Big Oil
stunning breakthrough in chemical engineering has unleashed a massive supply
of fuel…
Enough fuel, in fact, to power the entire globe for over 36,000 years.
It’s poised to decimate Big Oil’s obscene profits, make OPEC obsolete, and
hand the United States 100% energy independence.
In fact, the U.S. Department of Defense just invested $7 billion in a single
day…
Apple, Google, and Facebook are spending billion... 阅读全帖 |
|
h**********9
发帖数: 3252 | 9 是dilute了，不过用增发的钱还了一半的债，而且跌下来的百分比超过dilution。 |
|
w*j
发帖数: 6104 | 10 September 30, 2016:
· Revenue was $20.5 billion GAAP, and $22.3 billion non-GAAP
· Operating income was $5.2 billion GAAP, and $7.1 billion non-GAAP
· Net income was $4.7 billion GAAP, and $6.0 billion non-GAAP
· Diluted earnings per share was $0.60 GAAP, and $0.76 non-GAAP
https://www.microsoft.com/en-us/Investor/earnings/FY-2015-Q1/press-release-
webcast
October 23, 2014 — Microsoft Corp. today announced revenue of $23.20
billion for the quarter ended September ... 阅读全帖 |
|
b****y
发帖数: 480 | 11 In November 2013, Nvidia sold about 64 million warrants to Goldman Sachs.
Today, both parties are entering in a termination agreement, whereby Nvidia
will settle in shares.
The termination agreement will cost Nvidia shareholders anywhere from 8% to
9.2% in dilution.
This is a major dilutive transaction and therefore negative for the stock. |
|
r*****e
发帖数: 7853 | 12 NetEase (NTES) Tops Q2 EPS by 4c, Beats on Revenues
August 9, 2017 7:13 PM
NetEase (NASDAQ: NTES) reported Q2 EPS of $3.86, $0.04 better than the
analyst estimate of $3.82. Revenue for the quarter came in at $1.97 billion
versus the consensus estimate of $1.9 billion.
Second Quarter 2017 Financial Highlights:
Net revenues were RMB13.4 billion (US$2.0 billion), an increase of 49.4%
compared with the second quarter of 2016.
Online game services net revenues were RMB9.4 billion (US$1.4 billion), an... 阅读全帖 |
|
|
w*****e
发帖数: 931 | 14 刚从seekingalpha转贴了个貌似有错，应该是双beat
BEIJING, Nov. 15, 2017 /PRNewswire/ -- NetEase, Inc. (NTES) ("NetEase" or
the "Company"), one of China's leading internet and online game services
providers, today announced its unaudited financial results for the third
quarter ended September 30, 2017.
Third Quarter 2017 Financial Highlights
Net revenues were RMB12.5 billion (US$1.9 billion), an increase of 35.5%
compared with the third quarter of 2016.
Gross profit was RMB5.9 billion (US$893.9 million), an incre... 阅读全帖 |
|
m******n
发帖数: 15691 | 15 2017年， GM 在中国卖了 4M 辆，北美 3M
2018 1Q
First-quarter 2018 results:
EPS-diluted of $0.77 and EPS-diluted-adjusted of $1.43
Revenue of $36.1 billion
GM North America EBIT-adjusted of $2.2 billion and margin of 8.0 percent
GM North America is on track to sustain 10 percent full-year margin
GM International EBIT-adjusted of $0.2 billion, includes record equity i
ncome in China of $0.6 billion
GM Financial EBT-adjusted of $0.4 billion, nearly double year over year |
|
s***r
发帖数: 1121 | 16 Operating income (EBIT) 217,822.74
Net Income 82,661.80
EPS 0.49
============
TSLA Q3E
Automotive sales 6,010,680.00
Automotive leasing 286,158.00
Total automotive revenues 6,296,838.00
Development services and other 304,281.00
Energy generation and storage 317,505.00
Total revenues 6,918,624.00
% of auto revenues 0.91
% of development service and other 0.04
% of energy generation and storage 0.05
Revenue growth rate 132%
Automotive sales... 阅读全帖 |
|
s***r
发帖数: 1121 | 17 Q4 estimated EPS: 0.79
Auto revenue: 6,547,000.00
Total revenue: 7,426,140.00
EBIT: 286,839.92
Net income: 134,424.69
Assume:
Estimated M3 production no. 72,000.00
Estimated M3 delivery no. 85,000.00
no. M3 in transit 13,000.00
Estimated S delivery no. 12,000.00
Estimated X delivery no. 12,000.00
Price for M3 55,000.00
Price for S 75,000.00
Price for X 81,000.00
Esimated auto revenue 6,547,000,000.00
===============... 阅读全帖 |
|
发帖数: 1 | 18 这个是短期高利贷，不会有dilution.
：8个亿占60B 也就 1.6%， 毛毛雨了。dilute 也不是什么大毛病。short sellers
：always can find some excuses of course. 关键还是需求和生产能力。 |
|
i**p
发帖数: 940 | 19 如果公司搞的好的话，一般每一轮funding (after series A)也就是dilute 10％左右
。dilution基本是个non issue，主要是
看公司有没有前途。 |
|
l**t
发帖数: 6971 | 20
我也正想说这个呢。要是可以这么玩儿的话，以后startup雇人全都给大笔的option，
先骗进来再说。过一阵子看谁不顺眼dilute谁。
Skype的情况比较特殊，而且只是对个别executive。Zuckerberg也dilute了co-founder
。不过对小兵搞这种的，只有下三滥的startup才会做。Zynga都做这么大了，还计较这
几个小钱，连名声都不要了，这CEO太没眼光了。
人家Google的厨子凭什么不能拿几千万的股票？当时给人家的时候，肯定谁都没想到值
几千万的。人家有眼光或是运气take risk，拿几千万是应该的。患难的时候要用人，
给人家股票。成功了就反悔，说人家不配拿这么多，哪里有这种道理。 |
|
m*******i
发帖数: 8711 | 21 Square 共30M shares，Dropbox有470M shares。
上轮Square 酬了200M，post-round 3.3B valuation. Dropbox 250M, post-round 4.
3B valuation. Square has bigger dilution.
Square是Series D，Dropbox是Series B。Square的早期员工被dilution多得多。 |
|
r******o
发帖数: 23 | 22 【 以下文字转载自 Stock 讨论区 】
发信人: smxzr (十载青衫客，一朝白发人), 信区: Stock
标 题: TSLA Q3 Q4 最新预测 (10月2号最新预测
发信站: BBS 未名空间站 (Wed Sep 19 14:00:39 2018, 美东)
根据Q3 delivery number
最新预测：
Q3：
Total revenue： 6，411，984.00
EBIT 113，553.77
NI： 4，460.08
EPS： 0.03
Q4：
Total revenue： 7，227，200.00
EBIT 246，386.28
NI： 104，084.46
EPS： 0.61
==================
Operating income (EBIT) 217,822.74
Net Income 82,661.80
EPS 0.49
============
TSLA Q3E
Automotive sales 6,010,680.00
Automotive leasing 286,158.00
Total autom... 阅读全帖 |
|
|
S**U
发帖数: 7025 | 24 http://www.nytimes.com/2011/06/26/nyregion/the-road-to-gay-marr
在同性恋婚姻的争论中，我关注的是为什么基督徒认为他有权力剥夺同性恋结婚的权利。同性恋结婚合法化，完全不影响基督徒与异性结婚的权利呀。
至于 "dilute the value of traditional marriage", 这就好比常春藤联盟主张∶别
的大学不能叫 "university", 因为藤校才配叫 university, 其他大学也允许\被称为
"university", "dilute the value of university", 逻辑荒谬吧。
剥夺别人与己无关的权利是恶，无论是以上帝之名，或是打著道德的旗帜。
纽约州立法时，也考虑了宗教团体的立场，宗教团体如果出于信仰反对，可以拒绝为同性恋举行婚姻仪式，或出借场地。 |
|
c****g
发帖数: 3893 | 25 【 以下文字转载自 Belief 讨论区 】
发信人: SeeU (See you), 信区: Belief
标 题: 纽约州承认同性恋婚姻合法
发信站: BBS 未名空间站 (Sat Jun 25 12:55:16 2011, 美东)
http://www.nytimes.com/2011/06/26/nyregion/the-road-to-gay-marr
在同性恋婚姻的争论中，我关注的是为什么基督徒认为他有权力剥夺同性恋结婚的权利。同性恋结婚合法化，完全不影响基督徒与异性结婚的权利呀。
至于 "dilute the value of traditional marriage", 这就好比常春藤联盟主张∶别
的大学不能叫 "university", 因为藤校才配叫 university, 其他大学也允许\被称为
"university", "dilute the value of university", 逻辑荒谬吧。
剥夺别人与己无关的权利是恶，无论是以上帝之名，或是打著道德的旗帜。
纽约州立法时，也考虑了宗教团体的立场，宗教团体如果出于信仰反对，可以拒绝为同性恋举行婚姻仪式... 阅读全帖 |
|
b*****l
发帖数: 3821 | 26 another sharp comments:
http://forums.randi.org/showthread.php?t=88831
They could take several different approaches to proving homeopathy:
1) Prove that a properly controlled homeopathic remedy cures the proscribed
disease in a properly controlled double-blind experiment;
2) Prove that "like cures like", regardless of the potency of the remedy;
3) Prove that some of the substance remains in the solution after properly
controlled dilution, which flies in the face of Avogadro's limit and modern
ch... 阅读全帖 |
|
O*****c
发帖数: 679 | 27 Big pharm is in good shape
Pfizer:
Revenue (ttm): 48.84B
Revenue Per Share (ttm): 7.203
Qtrly Revenue Growth (yoy): 9.40%
Gross Profit (ttm): 37.18B
EBITDA (ttm): 20.42B
Net Income Avl to Common (ttm): 9.05B
Diluted EPS (ttm): 1.33
Qtrly Earnings Growth (yoy): 119.10%
JNJ:
Revenue (ttm): 63.57B
Revenue Per Share (ttm): 22.335
Qtrly Revenue Growth (yoy): 8.70%
Gross Profit (ttm): 43.34B
EBITDA (ttm): 19.00B
Net Income Avl to Common (ttm): 11.85B
Diluted EPS (ttm): 4.12
Qtrly Earnings Growth (yoy) |
|
x***q
发帖数: 4953 | 28 http://finance.yahoo.com/news/Apple-Reports-Third-Quarter-prnews-1588672529.html?x=0&.v=1
The Company posted record revenue of $15.7 billion and net quarterly profit
of $3.25 billion, or $3.51 per diluted share. These results compare to
revenue of $9.73 billion and net quarterly profit of $1.83 billion, or $2.01
per diluted share, in the year-ago quarter. Gross margin was 39.1 percent
compared to 40.9 percent in the year-ago quarter. International sales
accounted for 52 percent of the quarter's |
|
s****i
发帖数: 66 | 29 Pugh Co. reported the following in its statement of stockholders' equity on
January 1 of the current year:
Common stock, $5 par value, authorized 200,000 shares, issued 100,000 shares
$ 500,000
Additional paid-in capital 1,500,000
Retained earnings 516,000
2,516,000
Less treasury stock, at cost, 5,000 shares (40,000)
Total stockholders' equity $2,476,000
The following events occurred during the current year:
May 1 - 1,000 shares of treasury stock were sold for $10,000.
July 9 - 10,000 shares of ... 阅读全帖 |
|
h*******o
发帖数: 4884 | 30 Unless the antibody comes as lyophilized powder, it is recommended to store
the anitbody in un-diluted aliquots. Diluted antibody is not as stable as
the original stock.
For lyophilized powder, usually add PBS or ddH2O following the product data
sheet, then aliquot for storage. |
|
p****y
发帖数: 95 | 31 PEI Transfection
Transfection:
1. Split 293T cells one day before transfection in DMEM/10% FBS medium:
a. 6 well dish: 0.5x106 cells
b. 10cm dish: 4.0x106 cells
c. 15cm dish: 9.0x106 cells
2. Prior to transfection bring all reagents to room temperature.
3. In a sterile tube dilute total plasmid DNA (ug) in serum-free DMEM w/o
phenol red (volume of media is 10% of final volume in culture vessel). Use
transgene: viral packaging (psPAX2):viral envelope (pMD2G) constructs at 4:2
a.... 阅读全帖 |
|
c******r
发帖数: 3778 | 32 可以直接读OD，但是非常不准。
测titer最好还是limited dilution，pcr啥的，测的都是总virus，不是functional的
infectious unit。如果感染的ratio很重要，那还是要用dilution。 |
|
i**********5
发帖数: 261 | 33 dilute your template. dilute your template. often times, genomic DNA
solution contains tons of chemicals and proteins which are inhibitors of
your PCR |
|
s********n
发帖数: 248 | 34 我用过cell signaling 的pAKT T308,这个克隆是最好的
http://www.cellsignal.com/products/2965.html
block 用5%milk/PBST (0.02% TWEEN 20), dilute primary antibody in either 5%
milk or 5%BSA/PBST, 4 degree overnight.
quick wash 2/3 times, then wash 3 times*5 min with PBST,
dilute 2nd antibody (JACKSON LAB HRP-GOAT anti-Rabbit light chain) 1:2500 in 5%MILK/PBST
(如果杂带多，需要把二抗稀释在5%milk里), RT for 1h; 如果杂带不多，可以稀释1:
15000 in PBST, RT for 45min。
quick wash 2/3 times, then wash 5 times*5 min with PBST. |
|
D*a
发帖数: 6830 | 35 但是细胞实验也很难就把重复实验混合起来。比如dilution之类的，两天的dilution肯
定不一样啊。这怎么合得起来。。。
我看到几个教statistics的地方说了一大堆这个要这么弄那个要那么弄，什么这个也错
误那个不对的，最后来了句，最重要的还是瞅瞅你的数据长什么样儿。。。我心想，
TNND，看这么半天就学个这个。。。非要做统计学分析的不是我好不好。。。
rep |
|
l********s
发帖数: 70 | 36 最近做RNA从plate中提取，发现RNA测试有机溶剂那个参数很低，0.3-0.6 。 其实最后
我尽量把乙醇抽吸干净。我怀疑是不是DEPC水有问题，因为我统一吧RNA dilute 到
100ng/ul 做反转，发现dilute后，那个比值更低了。。。
当然Nanodrop 的control 用的是DEPC，这个不会搞错。。
不知道为啥，怎么提高这个比值？ 另外这个如果低多大程度影响反转？ |
|
c********b
发帖数: 363 | 37 每个步骤都是别人都知道的,我只是组装了一下. 最大不同是别人是先Urea变性,提出来
再复性.我是利用Triton X-100可以洗脱SDS的特点利用SDS变性,然后Triton X100洗脱
复性,然后过柱. 肯定不会对所有蛋白适用,但是起码我遇到的都work (包括一个>100
Kd的酶).
我是用的sonication water bath, 也可以用probe sonication再加SDS,都OK,但是注意
dilution的比例和体积.
Good luck. 用的高兴分我几个包子.
Key, keep a fraction from every step for analyses and trouble shooting
- Culture the E. coli overnight
- Induce it at a proper concentration of IPTG, room temperature for ~
4hrs
- Pellet the E. coli at 6000 rpm at 4 degree
- R... 阅读全帖 |
|
P****o
发帖数: 172 | 38 I usually put cells on ice before seeding, then pick up 10ul for dilution
and count. I prepare 3 counters one time, each counter can be loaded with 2
samples, so in total it is six. One sample only takes no more than 30
seconds if the dilution is optimal.
If cells can not be put on ice in some cases, automatic counter may be
better. |
|
a*******u
发帖数: 19 | 39 Cell的一篇paper
http://www.cell.com/abstract/S0092-8674(09)01506-2
的supplementary
Estimation of absolute number of Jarid2 and Jarid1a molecules per ES cell
nucleus.
Purified recombinant Jarid2 fragment a (Figure 2B, details in Table S9)
containing the epitope
recognized by the Jarid2 antibody was used for protein estimation. The
Jarid1a recombinant
protein containing aa191-290 (cat# H00005927-Q01) and the antibody (cat#
H00005927-A01)
recognizing same fragment were purchased from Abnova. Two-fold s... 阅读全帖 |
|
Y*****e
发帖数: 9 | 40 Mouse anti-luciferase monoclonal antibody from Sigma (Cat#: L2164-.2ML
), try 1:200 dilution for IHC or IF. Use 1:400 or higher dilution for
western |
|
c*********8
发帖数: 369 | 41 Thanks for reply.
High-quality 3.7% formaldehyde (diluted in buffer); incubate 15
minutes at room temperature. Rinse each sample in buffer 3–4 times for one
minute.
Permeabilize the cells in 0.2% Triton X-100 (diluted in buffer) for 5
minutes |
|
c********b
发帖数: 363 | 42 你的loading dye dilute 之后就是2% SDS啊，loading 没关系的，5%的我都用过，
dilute到2%也能跑。
一般如果是DNA binding蛋白最好上2%SDS, 不然就RIPA+Benzonase (多麻烦啊）。 |
|
i***0
发帖数: 160 | 43 If you dilute the high concentration protease to low concentration its
activity increase, then the decreased activity at high concentration is
reversible, which is good. It might due to the storage buffer you use for
concentration not friendly for the activity of the enzyme, or reversible
oligomerization that enclose the active site.
If you dilute the high concentration protease and can not restore the
activity you have seen while it is at low concentration, it is possible,
that it is aggregated... 阅读全帖 |
|
H****n
发帖数: 26 | 44 自己折腾了好久，还是解决不了问题，只好来版上请教大牛了。
DNA shearing to 150-700 bp, Input DNA 量是～100ug，抗体用Abcam的anti-HA （
ChIP grade， 5ug for each IP）
抗体结合的buffer 配方是（ChIP dilution buffer： 20mMTrisHC PH8.0， EDTA PH8.
0 2mM, NaCl 150mM, SDS 0.01%, TritonX-100 1%）. 抗体incubate Overnight,
millipore的beads用dilution buffer 洗三遍，然后BSA block overnight 第二天加进
去，3-4hours.
还有，做的是tissue的ChIP（表达这个TF的细胞只占细胞总数的8%-10%），为了让固定
的1%甲醛渗透更快点，用tripsin消化了
组织（之前直接用SDS裂解组织也做过，效果也不好），固定10min.
wash： 1 low salt ->1 high salt->1 LiCL suffer->2* TE
问题: 1.... 阅读全帖 |
|
l**********1
发帖数: 5204 | 45 En
温习一下 其十一年前的豪言 啊
中国科学：显著的发展和严峻的挑战
——历史演变和现状比较
于2001年12月4日 1st version
饶毅
本文在简要回顾中国科学史的基础上，介绍一些近年研究的内容，肯定中国
科学令人乐观的进步，并讨论可能的意义。同时也指出，中国优秀论文总量仍不
到世界的百分之一，低于中国经济在世界所占的百分比、也不能适应中国持续发
展的要求。中国科学的规模需要相当程度的扩大、质量有待进一步提高。中国科
技还存在面临许多问题和挑战。
中国科学历史上的优秀例子
一个国家科学研究状况可以近似地由发表论文的情况所反映。以下，本文主
要从生命科学的研究来讨论中国科学的情况，一方面这是我有一定判断力的领域，
另一方面生命科学是科学技术最重要的组成部分之一，可以反映科学主流。讨论
中国论文发表情况前，先谈两个背景：中国科学的历史情况，优秀科学和著名杂
志的关系。
奠定中国生命科学研究是二、三十年代协和医学院生理系林可胜和生化系吴
宪。他们不仅自己研究出色，而且培养和带领了其他研究者。林可胜在胃肠道生
理和神经生理有优秀工作。1942年，他在中国当选为美国科学院外籍院士，是... 阅读全帖 |
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t******k
发帖数: 599 | 46 看到很多protocol里面sonication，离心以后都要把supernatant用dilution buffer稀
释，然后分装n管子。因为我的样品本来就不多，所以没有dilution，直接拿去做ip，
这样会有问题吗？ |
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i***0
发帖数: 160 | 47 To be honest, according to my experiences, the worst reliability of a plate
reader is the absorption assays. I have tried Bradford or OD280, both give
high standard deviation. However, if you just want a guess, try using your
sample at original conc, 1:1 dilution, and 1:4 dilution in triplicate. You'd
better have BSA standards at least 5 concentrations in the same plate in
triplicate. These will give you a relatively reliable readout. OD is linear
at 0.5 to 1, roughly. good luck |
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c********r
发帖数: 189 | 48 should be 2X dilution instead of 10X dilution? |
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l****b
发帖数: 400 | 49 It is difficult to remove the fat, but not impossible. Spin first, then use
gentle vacuum to remove the top layer, which contains mostly lipids. Repeat
the process if necessary. The remaining lysates can be further diluted to
reduce the lipid concentration. Since liver samples generally have plenty of
proteins so diluting them would be OK. It is a pain in the neck but with
this method my liver western blots, even with samples from high fat fed mice
, look perfectly fine. |
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