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b******r
发帖数: 1137 | 3 it's out on 21st: Star Wars The Force Awakens 2015 HD-CAM XViD HQMic AC3-CPG |
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r*g
发帖数: 3159 | 4 Cam的意思就是影院偷拍，camera,不管前面待不带hd.
CPG
[发表自未名空间手机版 - m.mitbbs.com] |
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l*******g
发帖数: 1263 | 6 lz有没有搞错啊？你自己才是小众啊。
我是80年代初的生的人，小时候只听欧美和港台的流行音乐，北京的小众摇滚乐，说实
话只听过名字。我的同学朋友们莫不是如此。
现在90后喜欢听什么，看看参加好声音的两个国外呆过的90后就知道了。
究竟什么是大众，你去查查演唱会的场次和观众，各大CPG品牌的代言人，就知道了。 |
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l*******g
发帖数: 1263 | 7 看看它是不是可口可乐，百事可乐，宝洁和联合利华的代言人就行了。
这些CPG公司market research很强的，很知道谁代表了中国真正的消费潜力。
有啥好争的？ |
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n******t
发帖数: 239 | 8
你去找根据coppermine 发展起来的 CPG Nuke 。 |
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B*****g
发帖数: 34098 | 9 show off啥呀，啥都找我做，我宁愿不会。我现在已经很收敛了，别人干傻事我都不吱
声，点名问谁做俺也不接茬，可还是躲都躲不开。cpg的事干多少都没用，过了没人记得
住。多干不多得，写垃圾code也挣那么多钱，大锅饭，这国家早晚废掉 |
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c********e
发帖数: 598 | 13 In 2000, Chu 的重要结论是DNA-PK参与了CpG DNA 诱导的天然免疫反应.
Chu "obtained biochemical and genetic evidence that DNA-PKcs is indeed
required for signaling events that lead to induction of innate cytokines,
such as IL-6 and IL-12. We demonstrate that bacterial DNA and ISS-ODN
activate DNA-PKcs, which can phosphorylate the IKKβ subunit of the IKK
complex to cause NF-κB activation. Both DNA-PKcs and IKKβ are essential
for induction of cytokines in response to bacterial DNA and ISS-ODN."
In 2002, FDA, NCI 的Dennis |
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p*****m
发帖数: 7030 | 14 我觉得这就是computational path的人和传统生物学家的区别把。要我看movement
或者说locomotion类型的behavior最无聊 因为就是单调的stereotyped没什么趣味
我也想不出什么更复杂的circuit或者CPG来控制这种东西。但是让一个计算背景的
人看 也许他们会觉得只有这些behavior才值得作 因为高度quantitative
Most
thus |
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E****A
发帖数: 184 | 15 I tried once, only loaded 60ug total RNA of each sample for mRNA isolation,
however still some 28S and 18S rRNA left, as one of the probes located in a
CpG island, lots of the probes cross-hybridized with the left rRNA,
frustrated! |
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F***w
发帖数: 58 | 16 请好心人帮我下载一篇paper全文
Differential methylation of tissue- and cancer-specific CpG island shores
distinguishes human induced pluripotent stem cells, embryonic stem cells and
fibroblasts
Nature Genetics 41, 1350 - 1353 (2009)
信箱 b*****[email protected]
非常感谢！！！ |
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k*******s
发帖数: 214 | 17 想分析一下么CpG islands. 请推荐好用的预测软件。谢谢 |
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S**********e
发帖数: 1789 | 18 看了这个教授的主页，竟然公布了自己review的文章，题目，杂志名称都有，是不是违反职业道德呢？
Editorial Activities
Journal Peer Reviewer
2009 International Journal of Cancer. (Article) E-cadgerin
transcriptional down-regulation by epigenetic and microRNA-200 family
alterations is related to mesenchymal and drug-resistant phenotypes in human
breast cancer cells, IJC-09-1835.
2009 Molecular Cancer Therapeutics. (Article) Role of reactive oxygen
species in the ability of H-Ras to enhance cell death induced by histone
deacetylase inhibit... 阅读全帖 |
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d****i
发帖数: 2346 | 19 刚开始做启动子甲基化。
像这个图中的1、3、6行是不是可以看作是明显的没有convert的？这样我在计算甲基化
比例的时候应该把几个序列exclude掉？ |
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b****r
发帖数: 17995 | 20 同时做全部都没有被甲基化的control，比如pcr product，如果都converted那就说明
问题了 |
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d****i
发帖数: 2346 | 21 谢谢各位！
我是发现那几行每个CG位点有没有变，因此有疑问。 |
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t**k
发帖数: 16 | 22 哈哈,多谢高人指点,看来先知道是否是CpG island 是第一步,如果没有甲基化,就白撤
了,如果原来有甲基化,就还有点希望.
我现在就是觉得甲基化如果在C上,G突变了,好像意思不大吧. |
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s******s
发帖数: 13035 | 23 1.另一条链是C
2.不是CpG, DNMT1没法maintain甲级化 |
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b*******n
发帖数: 605 | 24 请问想查基因上游调控区所有可能的binding位点， 用什么free软件最最最好？
查CpG island有什么free的软件？
拜谢！ |
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R****n
发帖数: 708 | 25 you are talking about Methylation Specific-HRM, I assume. Althought you are
using qPCR system, you didn't use a beckman probe to identify the sequence
change. So this is not a qPCR method.
ZymoResearch's kit should be fine. We used their kit a lot. There are some
commercial M or UM DNA standard which have been converted. You can use them
to test your primers.
How do you design your primers? flanking any CpG sites?
If you didn't change primer, I guess that you may have PCR product
contamination i... 阅读全帖 |
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D*a
发帖数: 6830 | 26 被reviewer郁闷到了，我们的breeding strategy，他说为啥只用公的+/-,有一篇paper
说公老鼠的diet影响后代的metabolic gene expression。
下了paper一看，他们把老鼠pups养到三周杀了，做了一堆microarray和CpG什么的。唯
一的一个蛋白质实验是在肝里测了10个lipid，有三个的变化的p小于0.05
我就郁闷了，你多养几个星期抽个血测测insulin和glucose tolerence能死么，又不是
很难做。这些红点儿绿点儿的，到底有多少真能出来phenotype?!!!
真想直接criticize这篇paper。。。还是Cell的。。。 |
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b****r
发帖数: 17995 | 27 反了吧，是cre被甲基化了吧
loxp那么小的东西，有CpG island吗，能被甲基化吗？就是有几个C被甲基化了也没啥
大不了的
吧，难道就不能被切出来了？
cre这种大片段带promoter的东西被silent我倒是一点都不奇怪，其实基因组里大部分
东西都是有
潜在被表达的能力的，只是大部分被somehow抑制了而已 |
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z*t
发帖数: 863 | 28 try to“抛砖引玉”
For tools
The most fashionable things nowadays are tons of IP and related high
throughput sequencing ChIP/Me-IP/hm-IP, the application of hybridization
chips are not as popular as several years. But take DNA methylation(the only
staff I'm familar with:)) as example, the Me-IP can highly enrich promoter
region with dense CpG, the hybridization chips developed by Andy Feinberg
are more unbiased. But as everyone knows, the best way is whole genome
bisulfite sequncing, which is expensiv... 阅读全帖 |
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c***c
发帖数: 113 | 29 Thank you all very much.
Our purpose want to the methylation status at the promoter region of
interest gene, we may evaluate several CpG islands at different time points.
I have checked the onestep qMethy kit from Zymoresearch, it looks good. Did
anyone here use this kit? |
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c**g
发帖数: 14 | 30 我搞结构的，对这个不熟，但是我个人不认为这种去甲基化方式会是细胞内的主流，引
用上面的话，“在原始生殖细胞发生及受精后父源基因组的重塑过程中出现的大规模的
去甲基化，被认为是擦除父母甲基化谱式并建立后代新的甲基化谱式的关键”， 细胞
内那么多CpG, 那么多甲基化的C，如果都用这种方法在原始生殖细胞阶段擦除，会产生
多少abasic位点，后续的修复过程一是不经济，而是会对基因组稳定性产生很大的影响
，所以我个人觉得这个可能只是一条路。
欢迎大家给我扫盲 |
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j*****u
发帖数: 186 | 31 这篇文章： http://www.nature.com/ng/journal/v37/n8/abs/ng1598.html
里说we used an immunocapturing approach followed by DNA microarray analysis
to generate methylation profiles of all human chromosomes at 80-kb
resolution and for a large set of CpG islands.
这个80kb resolution 什么意思？
现在bisulfite sequencing可以是single nucleotide resolution, 这个80kb
resolution是只能精确到80kb一段DNA的平均methylation值吗？如果这样的话不是太不
精确了？我是不是理解错了？大家讨论下，谢谢 |
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s*********t
发帖数: 600 | 32 我需要从细胞系里面克隆一个promoter。
首先，能不能提供一个从细胞系提取基因组DNA做模版的protocol？
其次，我要克隆的启动子是CpG rich的区域，对于这种克隆，有没有什么窍门？记得版
上以前讨论过，当时没留意，找不到了。
能否指点一下？
非常感谢！ |
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l********s
发帖数: 70 | 33 他说的是不是 CpG岛预测？？ 不知道了，反正做bsp比较麻烦。 主要是ta克隆
谢谢楼上大哥 |
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n********k
发帖数: 2818 | 34 This is a smart, very focused and productive one I have heard from people
working with him...XD Wang's first student was even more impressive if I am not too old:) and several from HJ Song's lab also have impressive records, A and P from this board both have impressive records in pretty shoret time too...
BTW, to upbeat/Q a bit for those (including myself) who may feel depressed now)....Nothing particularly/unusually, Pretty much decent intelligence, strong motivations/working/person habits/... 阅读全帖 |
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a***f
发帖数: 32 | 35 Thanks to Lot..., Wak.... and Shak... again!
Sorry for not responding sooner. I did RT-PCR, as expected, the human
endogenous gene is expressed. But the mouse gene (BAC)is not.
I guess it is correct to say that the mouse transgene should have decent
chance to be expressed if the human orthorlogue is expressed, as a general
rule. But for a given gene, it is very hard to be sure. At least, the
organization of CpG elements and regulatory sequence of gene are very
different from mouse to human, ri... 阅读全帖 |
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k****z
发帖数: 1863 | 36 coverage varies between regions, such as CpG, promoter, regulatory regions,
CDs, so accuracy will also change |
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l**********1
发帖数: 5204 | 37 LZ 要找个mentor lab 问下对方 啦
比如 Fan GP of UCLA:
Fouse, S. et al. and Fan, G. (2008)
Promoter CpG methylation contributes to ES cell gene regulation in parallel
with Oct4/Nanog, PcG complex, and histone H3 K4/K27 trimethylation..
Cell Stem Cell 2: 160-169.
//faculty.bri.ucla.edu/institution/personnel?personnel_id=45766
and his group one N sister paper about DKO mice strain:
Feng J et al. and Fan G. (2010)
Dnmt1 and Dnmt3a maintain DNA methylation and regulate synaptic function in
adult forebrain ne... 阅读全帖 |
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s******s
发帖数: 13035 | 38 你咋设计的
给你几个hint
1.不要设计在CpG的地方，这个你应该知道
2.不要像普通PCR一样按照两条链设计，必须按照一条链设计
3.不要太长，200－300足够了
4.用Hifi Taq，普通酶和phusion有时候不work |
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l*******y
发帖数: 3987 | 40 搭车问个问题，是不是所有的C都有可能被甲基化？以前一直以为CpG位点才能被甲基化
的。 |
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t*****t
发帖数: 773 | 41 Most CpG island-methylated genes are germ-line-
specific genes or transposable elements.
---------
这个信息很重要，谢谢！ 先前我倒没有注意到 |
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b****r
发帖数: 17995 | 42 就是说一个island里的CpG，要不都被甲基化，要不都不甲基化，不一致的情况总是比
较少的，比如说一个C甲基化，一个不甲基化，一个甲基化，一个不甲基化这样的情况
我反正没见过 |
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b****r
发帖数: 17995 | 43 CpG里的C被甲基化的可能性要高多了，也比较稳定，比较conserve |
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b***h
发帖数: 26 | 44 问个相关问题： CpG 和 GpC 中，哪一个甲基化强？为什么？谢谢。 |
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b***h
发帖数: 26 | 45 甲基化频率 和 repressive effect。
Is GpC endogenous abundance lower than CpG? Thanks. |
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i**y
发帖数: 71 | 47 it doesn't happen much in metazoan cells unless it's GCG. CpGs are the
canonical substrates for DNA methyltransferases.
Repressive effect is currently unknown. |
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a*******a
发帖数: 4233 | 48 不只是CpG, 大多数C都能被甲基化，
好像09年ren bing出过篇文章， base solution mC xxxx 神马的
记不清了 |
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s******s
发帖数: 13035 | 49 感觉应该opposite吧？CpG被甲基化以后有变成TpG的危险，
所以频率应该低于多数其他的dinucleotide |
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C********4
发帖数: 308 | 50 这个说法不是错的么
以前说ES里有很多单个的C（非CpG）的甲基化
但是Dirk Schübeler去年的那篇nature不是这么个结论？ |
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