KV
发帖数: 5728 | 1 听大佬说了，祝你们明天钓的好，我们新放船那两个新湖，如果八月份有时间，和你们
去逛逛，呵呵。
对了，刚收到一位大佬站内短信，提醒真空机去气泡的一点问题，我贴上来，帮忙看看
？有没有好的办法？真空度怎么控制？仪器测量？我买了真空机和封闭容器，但是没有
带压力表。
-------------------
标 题: Re: Re: 千锤百炼，精工打造，一流品质
发信站: 未名空间 (Fri May 17 14:35:14 2013)
KV，看到你关于真空环氧脱泡的问题，提醒你注意真空度不要超过28inHg，时间不要超
过1分钟，因为环氧固化剂本身在负压下会挥发，如果你看到有气泡持续出来，说明很
卡能不是气泡，是固化剂。
我倒是建议你试一下离心脱泡，用下面连接的mini centrifuger，和eppendorf vial，
高速离心15分钟，保证气泡free，而且如果环氧中有杂质，也同时被离心到底部，2毫
升的vial分装，可以节约环氧，不用的，盖好盖，放在-20度冷冻，可以用很久，只是
注意买离心机的时候，看是否跟塑料vial 匹配。
http://www.ebay.com/sch/i... 阅读全帖 |
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c*********o
发帖数: 8367 | 2 Reviews
Top 10 Baitcasting Reels
By Jesse Schultz 15 comments
Looking for some new baitcasting reels? Today’s economy forces us to
rethink the way we buy fishing equipment. The best “bang for your buck” is
looked at a little differently today. Our buck isn’t quiet taking us as
far as we would like it to go. To help you with your research, I’ve
compiled 10 of my favorite reels with the criteria in mind of both price and
performance. We all want to have the best equipment on the market so here
is ... 阅读全帖 |
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W*******s
发帖数: 18705 | 3 Daiwa Luna Tackle Warehouse Review:
Comments: By far my favorite round reel now that the Shimano TE's are
history. Performs better in all aspects than the Shimano Calcutta B's and
it is more ergonomic than the new D's. I am a long time Shimano user, but
better is better! The magnetic brake works very well and is easy to adjust.
If you need a functional piece of modern art to use on big, brutal fish,
this is the reel.
From: South, FL
Comments: I use this reel for swimbaiting, mostly at Diam... 阅读全帖 |
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c***3
发帖数: 85 | 4 得到willy和南非渔民启发，好想试试baitcast surf fishing。最近得了个鼓轮，abu
ambassadeur 7000c3，无论怎么调低brake（lure可以完全自由坠落）就是只能投个7-8
米搞得我焦头烂额，有几个问题请各位多指教了。
1.centrifugal brake到底怎么调，如图所示有4个pin，但是根本不想网上说的有开关
，完全自由的在那轴上动，上下滑起来非常非常的松根本没有卡住的感觉，这情况对么？
2.那个带刻度的圆盘有啥用？
3.到底应该调节哪边的刹车螺丝，到底是手柄那侧的那个还是手柄对侧的那个（上图的
那个螺丝）
4.螺丝里面那个黄铜的垫片是干啥的？上方那个是我买的没有黄铜垫片，下图那个是原
装的有垫片。 |
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W********s
发帖数: 1705 | 5 下面是Amazon一个客户对Core50的评论：
注意，1/16 OZ鸡哥是有挂件的，实际重量接近1/8盎司。1/6 OZ的RosterTail，实际分
量1/3吧，亮片带钩滚珠是不算在1/6OZ范围内的。
杆是Medium Light，他说的这个数据靠普实在。
Paired the Core 50MG7 with a Bass Pro Shop (BPS) medium lite CL66MLTF 6'6"
rod. With this combo and 8lb Yozuri hybrid line, I'm easily, consistently,
and accurately able to cast a 1/16 oz bitsy bug jig with a #3 Colorado spin
blade trailer 50 to 60 feet into a five mph breeze. Casting light lures on
this reel is so much fun, I don't care that I haven't caught any fi... 阅读全帖 |
|
发帖数: 1 | 6 甩杆距离除了跟用什么reel, 什么power/action的杆子，什么样多重的线，跟诱饵的空
气阻力也是有很大关系的。没怎么专门去钓crappie，但根据钓bass的经验，一个大浮
漂的风阻实在是太大了，后面再加上一截鱼线和诱饵（不知道你用什么诱饵），风阻可
想而知。如果浮漂只是用来保持在某一water column，那不如用Dropshot吧，风阻会小
很多，Dropshot在spinning outfit上可以甩挺远的。另外，1/2oz的重量对于ML的杆子
，是有点吃力了，还没算鱼钩鱼饵的重量。不如用Medium Power/Moderate Action的杆
子，hookset的时候同样也有一定的缓冲，不至于把如纸薄的Crappie嘴勒破了，但甩杆
时有足够的backbone，杆子也可以load up的更好，甩更远。而且，准确性肯定比ML的
要好。
至于Spinning和baitcaster哪个甩的更远，网上很多人在争，但事实是，甩杆距离的世
界记录都是baitcaster保持的。Dropshot也许不太适合baitcaster, 但我用1/4~1/2的
jerkbait也经常... 阅读全帖 |
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j****3
发帖数: 682 | 7 谢谢分享经验！我也有个low profile的royal legend, 仔细看了看，spool 与frame没
有Rover大，暂时勉勉强强用着。它的centrifugal 应该就是把四个或者两个小塑料片
往中间拨（好像和别的一些牌子是反的），增大摩擦。 |
|
j****3
发帖数: 682 | 8 是的，突然觉得它这个centrifugal 是靠“挂羊头卖狗肉”！
至于magnetic brake, 我的理解是它不是固定的，而且速度越快，阻尼越大。速度小的
时候（刚刚开始甩出时）作用几乎没有，主要作用在后面（限制spool 的最高速度），
应该是很有利于防止backslash. |
|
j****3
发帖数: 682 | 9 谢谢分享经验！我也有个low profile的royal legend, 仔细看了看，spool 与frame没
有Rover大，暂时勉勉强强用着。它的centrifugal 应该就是把四个或者两个小塑料片
往中间拨（好像和别的一些牌子是反的），增大摩擦。 |
|
j****3
发帖数: 682 | 10 是的，突然觉得它这个centrifugal 是靠“挂羊头卖狗肉”！
至于magnetic brake, 我的理解是它不是固定的，而且速度越快，阻尼越大。速度小的
时候（刚刚开始甩出时）作用几乎没有，主要作用在后面（限制spool 的最高速度），
应该是很有利于防止backslash. |
|
e*****n
发帖数: 12 | 11 Lowfat milk is made by removing fat with centrifugal force until the correct
level of butterfat is achieved. This is the standard process used to
produce varying fat levels in milk.
The fat-soluble vitamins A, D, E, and K along with essential fatty acids
such as linoleic and linolenic acid are found within the milk fat portion of
the milk. Milk fat contains glycosphingolipids, types of fats linked to
immune system health and cell metabolism.
You discard all of the health benefits when you discar... 阅读全帖 |
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m****8
发帖数: 327 | 12 自从对ball flight和swing plane的理论充分理解以后，抗忽悠能力大大提高。看了这
么多golf的书，个人认为hogan是最靠谱的，最有科学分析的能力，hogan做教练应该是
个好教练。Nicklaus的文化水平最差，他的书以感觉为主，深度有限。说不出为什么要
如此这般，一旦尝试去说，往往露馅。所以tiger说只有hogan和moe norman own their
swings。而对nicklaus表面尊重，背后并不屑。
只要开口说什么centrifugal force的教练，基本是不懂物理的。真正的好教练，其实
不多，我发现要找好教练，可能去找那些教业余或中学大学比赛选手的教练最靠谱。这
些人没功夫是没法混下去的，而course里的教练，很好混。 |
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f**s
发帖数: 2225 | 13 bu da slug jiu san le
li xin li, centrifugal force |
|
v***l
发帖数: 23 | 14
the new type of skis is also called:
parabolic skis, super sidecut skis, shaped skis
I don't know what new binding skis are.
do you mean those skis that are modified for supercarving?
they add some chips under the normal binding for more
aggressive carving to leave deeper tracks
as you ski at high speed, if you still make
carving turns, then the centrifugal force will
make your body tilt heavily inside. in that case, your
inside leg will have to support more weight for proper
balance (otherwise |
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a***y
发帖数: 19743 | 15 如果你喜欢听古典的话
更要找他的The BQE来听啊
Movement VII (Finale): The Emperor Of Centrifuge
很古典很棒 |
|
m*p
发帖数: 1331 | 16 国庆日和lab的几个老中，老韩，老美万年生物泼石岛 跑pcr期间 去看了场imax的
Jurassic World 。搞了个visa的10$ off 加上student id discount，反正卖票妹子不
查年龄。
万年生物泼石岛最震撼吓尿的，不是特效，不是剧情，而是Dr. Woo的那个lab！
想想自己在lab做的都是搞Drosophila, xenopus,顶多上个rat，平时也跑跑胶，写
script，弄pcr，顶多弄个chipseq好发paper， 看看人家 Jurassic World 的 Dr. Woo
的lab， 做的是最牛b的 synthetic biology， 太他娘的高大上的，building slick采
光足不说，仪器也是目测清一色最贵的，连Centrifuge也是futuristic。做的东西
visibility高不说，发nature，science肯定轻松，不想现在搞一个plos就谢天谢地，
eb1a也遥遥无期。。。。
看完后大家内牛满面，默默走出imax，走向parking lot 的那辆1998年camry。 终于老
中开口了，"how much... 阅读全帖 |
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b*s
发帖数: 82482 | 17 茶总这样研究吃，看起来是要走向molecular gastronomy的路子啊。不知有没有看过
Nathan Myhrvold的Modernist Cuisine: The Art and Science of Cooking？
In Modernist Cuisine: The Art and Science of Cooking, Nathan Myhrvold, Chris
Young, and Maxime Bilet--scientists, inventors, and accomplished cooks in
their own right--have created a six-volume, 2,400-page set that reveals
science-inspired techniques for preparing food that ranges from the
otherworldly to the sublime. The authors and their 20-person team at The
Cooking Lab have achi... 阅读全帖 |
|
b*s
发帖数: 82482 | 18 茶总这样研究吃，看起来是要走向molecular gastronomy的路子啊。不知有没有看过
Nathan Myhrvold的Modernist Cuisine: The Art and Science of Cooking？
In Modernist Cuisine: The Art and Science of Cooking, Nathan Myhrvold, Chris
Young, and Maxime Bilet--scientists, inventors, and accomplished cooks in
their own right--have created a six-volume, 2,400-page set that reveals
science-inspired techniques for preparing food that ranges from the
otherworldly to the sublime. The authors and their 20-person team at The
Cooking Lab have achi... 阅读全帖 |
|
s***n
发帖数: 1114 | 19 有男怀春，斯女诱之，夜半无人，共处斗室。
有男怀春，斯女诱之，纤纤玉手，centrifuge。
有男怀春，斯女诱之，鼓瑟其鸣，但求一日。 |
|
H********g
发帖数: 43926 | 20 可能是这些共面行星和太阳都是同一团云凝聚成的吧。
http://curious.astro.cornell.edu/question.php?number=205
Rings and disks are common in astronomy. When a cloud collapses, the
conservation of angular momentum amplifies any initial tiny spin of the
cloud. As the cloud spins faster and faster, it collapses into a disk, which
is the maximal balance between gravitational collapse and centrifugal force
created by rapid spin. The result is the coplanar planets, the thin disks
of spiral galaxies, and the accretion disks around... 阅读全帖 |
|
q**x
发帖数: 1636 | 21 【 以下文字转载自 WaterWorld 讨论区 】
发信人: mbp (Mac Book Pro), 信区: WaterWorld
标 题: 万年生物泼石岛看了Jurassic World 内牛满面。。。
发信站: BBS 未名空间站 (Sun Jul 5 12:57:33 2015, 美东)
国庆日和lab的几个老中，老韩，老美万年生物泼石岛 跑pcr期间 去看了场imax的
Jurassic World 。搞了个visa的10$ off 加上student id discount，反正卖票妹子不
查年龄。
万年生物泼石岛最震撼吓尿的，不是特效，不是剧情，而是Dr. Woo的那个lab！
想想自己在lab做的都是搞Drosophila, xenopus,顶多上个rat，平时也跑跑胶，写
script，弄pcr，顶多弄个chipseq好发paper， 看看人家 Jurassic World 的 Dr. Woo
的lab， 做的是最牛b的 synthetic biology， 太他娘的高大上的，building slick采
光足不说，仪器也是目测清一色最贵的，连Centrifug... 阅读全帖 |
|
C****5
发帖数: 54 | 22 【 以下文字转载自 WaterWorld 讨论区 】
发信人: mbp (Mac Book Pro), 信区: WaterWorld
标 题: 万年生物泼石岛看了Jurassic World 内牛满面。。。
发信站: BBS 未名空间站 (Sun Jul 5 12:57:33 2015, 美东)
国庆日和lab的几个老中，老韩，老美万年生物泼石岛 跑pcr期间 去看了场imax的
Jurassic World 。搞了个visa的10$ off 加上student id discount，反正卖票妹子不
查年龄。
万年生物泼石岛最震撼吓尿的，不是特效，不是剧情，而是Dr. Woo的那个lab！
想想自己在lab做的都是搞Drosophila, xenopus,顶多上个rat，平时也跑跑胶，写
script，弄pcr，顶多弄个chipseq好发paper， 看看人家 Jurassic World 的 Dr. Woo
的lab， 做的是最牛b的 synthetic biology， 太他娘的高大上的，building slick采
光足不说，仪器也是目测清一色最贵的，连Centrifug... 阅读全帖 |
|
s******c
发帖数: 1920 | 23 【 以下文字转载自 WaterWorld 讨论区 】
发信人: mbp (Mac Book Pro), 信区: WaterWorld
标 题: 万年生物泼石岛看了Jurassic World 内牛满面。。。
发信站: BBS 未名空间站 (Sun Jul 5 12:57:33 2015, 美东)
国庆日和lab的几个老中，老韩，老美万年生物泼石岛 跑pcr期间 去看了场imax的
Jurassic World 。搞了个visa的10$ off 加上student id discount，反正卖票妹子不
查年龄。
万年生物泼石岛最震撼吓尿的，不是特效，不是剧情，而是Dr. Woo的那个lab！
想想自己在lab做的都是搞Drosophila, xenopus,顶多上个rat，平时也跑跑胶，写
script，弄pcr，顶多弄个chipseq好发paper， 看看人家 Jurassic World 的 Dr. Woo
的lab， 做的是最牛b的 synthetic biology， 太他娘的高大上的，building slick采
光足不说，仪器也是目测清一色最贵的，连Centrifug... 阅读全帖 |
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c**i
发帖数: 6973 | 24 John de Jong, Dog owner wants to leave no stone unturned. Boston Herald, Mar
http://bostonherald.com/entertainment
/lifestyle/view.bg?articleid=1323037
My comment:
(a) the surname de Jong is Dutch meaning ‘the young’, for the younger of
two bearers of the same name--usually a son. The English and German
counterparts are Young and Jung, respectively.
(b) The vet stated, "Signs of urinary stones can include straining, bleeding
, etc."
That really hit me. When I was about 10 years old in City of Ka... 阅读全帖 |
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R******0
发帖数: 6158 | 25 查到一个制造商的回信，关于鱼油保质期的，据说是一年，所以小农决定先买4oz的试
试，如果效果好俩猫又能接受就上32oz的。。。
Production of Grizzly Salmon Oil and general info:
Grizzly Salmon Oil is extremely well protected from going rancid by nature
itself. A salmon has roughly 25% oil in its body mass and lives for several
years, so it has established a complex variety of natural antioxidants to
protect the oil, a.o. alfa-tocopherols also called vitamin E, when manmade
and asthaxantins. Our proprietary process for producing Grizzly Salmon Oil
is based o... 阅读全帖 |
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m****r
发帖数: 1904 | 26 The Bible’s Harmony With Scientific Facts. The Bible, at Job 26:7, speaks
of God as “hanging the earth upon nothing.” Science says that the earth
remains in its orbit in space primarily because of the interaction of
gravity and centrifugal force. These forces, of course, are invisible.
Therefore the earth, like other heavenly bodies, is suspended in space as if
hanging on nothing. Speaking from Jehovah’s viewpoint, the prophet Isaiah
wrote under inspiration: “There is One who is dwelling above t... 阅读全帖 |
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m***e
发帖数: 482 | 27 【 以下文字转载自 Whisper 讨论区 】
【 原文由 mouse 所发表 】
忘了当初作哪个试验了
是不是制氧气呀
用HCl的小管慢慢加
偶心急，一下加了好多滴
结果出来好多气体
就把它崩飞了
老师正好在偶旁边
大家一起看它飞上去又掉下来
挺有意思的。。。 |
|
|
r****o
发帖数: 105 | 29 如果前面那位兄弟只是选克隆的话(比如做stable transfected cell line)，稀释细胞
培养，然后慢慢挑就是。如果要直接从一大堆细胞选出一个符合要求的细胞，方法总结如
下。
From
Culture of animal cells: a manual of basic technique (4th edition)
(1)Sedimentation velocity at 1g
* based on cell size
*Custom-made separating funnel and baffles
*Simple technique but not very high resolution or yield
(2) Isopyknic sedimentation
* based on cell density
*centrifuge
* simple and rapid
(3) MACS
* Surface antibodies
* simple magnet and flow chamber |
|
|
s******y
发帖数: 28562 | 31 谢谢，但是这些策略对我们没有用，因为我们的蛋白是在细胞骨架里面的，
如果用温和裂解液的话，超过一半的蛋白就和actin filaments 一起在低速离心
下沉到pellet里了。要把所有蛋白都分出来的话我现在是用8M urea, 但是这样
就成了一个gel-like structure, 我也试过pre-clear step with beads，
但是发现根本没有用，因为整个溶液（就是一个胶体结构）都粘在resin bead上，
用普通的desktop centrifuge根本分不开。
release
down |
|
s******r
发帖数: 2876 | 32 能不能用drug抑制actin filaments?
谢谢，但是这些策略对我们没有用，因为我们的蛋白是在细胞骨架里面的，
如果用温和裂解液的话，超过一半的蛋白就和actin filaments 一起在低速离心
下沉到pellet里了。要把所有蛋白都分出来的话我现在是用8M urea, 但是这样
就成了一个gel-like structure, 我也试过pre-clear step with beads，
但是发现根本没有用，因为整个溶液（就是一个胶体结构）都粘在resin bead上，
用普通的desktop centrifuge根本分不开。
release
down |
|
|
d*****r
发帖数: 2583 | 34 周五晚上，你让大家都不务虚，这个基本上很难，:) |
|
b********r
发帖数: 709 | 35 今天拿到一个temporary research associate的offer,6个月，说是可能延长到10个月
。工资和博后差不多。自己买保险之类的话可能还要少一点了。
面试的时候和几个人谈了谈，感觉做的挺低级的，就做centrifugation，可能加一点
chromatography.不过这个工作的POSTING的要求只是本科毕业，一年实验室经验。
不知道这个工作对以后找工作有没有帮助，从技术上好像没有。另外没有电话面试就让
我去on-site,刚回来就有HR打电话说特别喜欢我，要我星期一就去orientation.
PS:正在原来老板的实验室做博后，OPT开始了几个月。没有找博后的动力，不过6个月
以后这个temporary工作结束，不知道怎么样。
求建议，多谢！ |
|
s*i
发帖数: 20 | 36 Change boss if you still can, seriously.
What you are doing is ok, you can even skip centrifuging. Sonicating and
heating in SDS buffer is enough. |
|
s******y
发帖数: 28562 | 37
Because radioactive detection is very sensitive.
If you use cell-free in vitro translation kit, you will stick a lot of
ribosomes on the NTA-resin, and the unfinished peptides will show up as
smear on the gel.
What you need to do, is to use high speed centrifugation (at least 144,000
xg) on a 20% sucrose cushion to separate the ribosome fraction (go to bottom
) from the complete proteins (remain on the supernatant).
Then, you can use the NTA resin on the supernatant. |
|
s******y
发帖数: 28562 | 38 You should NOT use the gel filtration because it will significantly dilute
your sample.
If you have a doubt about this, you should load the eluant directly without
any pulldown to see how much full length protein you recovered.
I don't know why you are bothered by the free amino acid. They will be
washed out in the pulldown process. I suggest you NOT to do the gel
filtration at all.
What you need to do is this:
1.centrifuge to pellet the ribosome. Are you sure you are using 100,000 rpm?
Not 100, |
|
s****a
发帖数: 436 | 39 Purification of biotinylated proteins on streptavidin resin: A protocol for
quantitative elution。
For elution of biotinylated proteins, 500 mL
release solution (2% SDS, 30 mM biotin, 50 mM phosphate,
100 mM NaCl, 6 M urea, 2 M thiourea, pH ,12)
were added to the resin and incubated for 15 min at RT,
followed by incubation for 15 min at 96C. Afterwards, the
resin was pelleted by centrifugation for 5 min at 16 100g |
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T*****u
发帖数: 3257 | 40 if you just want to pass the cell, you can just take some cells with the old
medium and add them to the new medium.
if you want to completely change the medium, y ou can centrifuge the cells
down and resuspend them in new medium. |
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s******y
发帖数: 28562 | 41 Why don't you use the convenient column?
I never bother to use centrifuge to spin down beads in big volumes.
It is always difficult to spin down the beads after binding with proteins.
5min |
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m****m
发帖数: 395 | 42 The reason why I didn't dialysis is that my protein is a hydrophobic
membrane protein which is desolved in an expensive detergent. If I dialysis,
the detergent will also go away and my protein will precipitate anyway.
I wonder if there's any other way to concentrate protein except using this
method and centrifuge through a membrane to get rid of the water? The later
is not so efficient, because I see some protein also pass the membrane which
causes loss.
Can we use vaccum dry under low temperatu |
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D**A
发帖数: 311 | 43 最近看文献，发现有的组说Mnase resistance chromatin其实是nuclear matrix，有的
组说其实那是condensed
chromatin, 有的组干脆直接说那个是heterochromatin。因为不在chromatin领域，不
知道该听哪个的？请各位同学帮
忙。
还有，Mnase digest，一般用EDTA/EGTA来terminate反应，那如果想要结束反应时，立
刻centrifuge 30s, 取
supernatant，那是不是也有同样的效果呢？毕竟mono nucleosome DNA是Mnase
resistant的。
谢谢 |
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h*****d
发帖数: 210 | 44 加蛋白probe是不一样的， it is smear, but there is not a single distinct
probe. Could have some reasons for this, first of all, probe denaturing and
annealing is not good, I bought oligonucleotide, because it is highly
purified. the free probe is also smear which is about half length of the
smear with protein.
secondly, when I extract nuclear protein, I just use 250M sucrose in the
extraction buffer for nuclei and no further gradient centrifuge thereafter.
High/low salt method for getting nuclear protein( |
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g*****y
发帖数: 6325 | 45 我以前做的membrane extract 的protocol 超级简单。 1. 用pbs resuspend 细胞，
sonicate; 2. 2000rpm 2min 取supernatant. 3. ultra centrifuge 28000 rpm 1hr;
4. 除去上清，用你的lysis buffer resuspend pellet. 然后加 loading buffer 跑胶
。 |
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g*****y
发帖数: 6325 | 46 如果IPTG不好用，试一下autoinduction。 还有你追加Amp一点用也没有，media里的酶
很快就全部掉了。你必须
centrifuge down cell, 加入新的medium. |
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v***a
发帖数: 1242 | 47 非常感谢
还有2个问题：
1）你是用什么buffer来incubate 1st Ab 和 beads的？跟wash一样的buffer吗？我
wash是用IP lysis buffer。你会preclean nuclear extract/cell lysate吗？
2）low pH glycine solution具体是多low？6.8？这个是可以直接elute然后
centrifuge了就可以上样跑胶？
with |
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s******y
发帖数: 28562 | 48 1. memrane filter? I would recommend using the inverted filter set in order
to protect the activities of the proteins.
2. high speed centrifugation with sucrose cushion?
3. a short, fat, gel filtration column? |
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