b******r
发帖数: 120 | 1 【 以下文字转载自 JobHunting 讨论区 】
发信人: bostoner (健身+读书), 信区: JobHunting
标 题: Job Position: Centrifugal Compressor Aerodynamic Engineer
发信站: BBS 未名空间站 (Fri Jan 21 18:09:14 2011, 美东)
注：
1)职位链接和具体要求贴在了下面,如果有些要求达不到，不要太在意，有些要求就是
浮云。。。职位的title和salary视你经验而定
2)这个是组里新增的职位，老板人很好，而且该职位对以后职业发展很有帮助。
3)组内有一个contractor（印度人），也会申请这个职位。他背景较弱，硕士学位
+ 1年工作经验（在本组）。所以，你最好有相关的背景，如果是硕士刚刚毕业+
无相关工作经验，我觉得比较难竞争过组内的contractor
4）你可以直接在网上申请，或是发简历给我（j********[email protected])，我再转发给HR
和我老板。请只选择一种做法，原因嘛，你知道的。。。
Link:
https://operations-elli... 阅读全帖 |
|
b******r
发帖数: 120 | 2 注：
1)职位链接和具体要求贴在了下面,如果有些要求达不到，不要太在意，有些要求就是
浮云。。。职位的title和salary视你经验而定
2)这个是组里新增的职位，老板人很好，而且该职位对以后职业发展很有帮助。
3)组内有一个contractor（印度人），也会申请这个职位。他背景较弱，硕士学位
+ 1年工作经验（在本组）。所以，你最好有相关的背景，如果是硕士刚刚毕业+
无相关工作经验，我觉得比较难竞争过组内的contractor
4）你可以直接在网上申请，或是发简历给我（j********[email protected])，我再转发给HR
和我老板。请只选择一种做法，原因嘛，你知道的。。。
Link:
https://operations-elliott.icims.com/jobs/2462/job
Overview:
For 100 years, the Elliott name has been synonymous with precision
engineering, extraordinary reliability and unparalleled service. Ellio... 阅读全帖 |
|
w******e
发帖数: 1187 | 3 请问有人见过用centrifugation pellet cell的recovery rate的研究吗？感觉应该是
很有用的信息。我总感觉低浓度的cell sample用centrifugation的recovery rate
应该很差，可是没有具体数据总是不安心呵呵
多谢！ |
|
M*******n
发帖数: 508 | 4 DNA molecule in solution, when centrifuging,
how to calculate the centrifugal force each molecule feel?
could anyone give me some advice? |
|
d****i
发帖数: 360 | 5 【 以下文字转载自 Chemistry 讨论区 】
发信人: dududi (dududu,Fourth grade driver), 信区: Chemistry
标 题: Ultrafiltration Centrifugal Device.
发信站: BBS 未名空间站 (Wed Sep 10 09:14:04 2008)
I need some ultrafiltration Centrifugal Devices with 1000 MWCO. Does anybody
happen to know such products and the manufacturer? Thanks! |
|
y****y
发帖数: 275 | 6 我们需要买一个continuous centrifuge， 大型的有commercialized的，但是我们需要
体积和容量小的，可以fit进我们实验室和试验台上的。不知道哪位兄弟有这个经验或
者信息的，google了半天没找到合适的。多谢多谢！ |
|
y****y
发帖数: 275 | 7 我们需要买一个continuous centrifuge， 大型的有commercialized的，但是我们需要
体积和容量小的，可以fit进我们实验室和试验台上的。不知道哪位兄弟有这个经验或
者信息的，google了半天没找到合适的。多谢多谢！ |
|
j*****a
发帖数: 92 | 8 Amicon ultra-15 centrifugal filter (10 K) 浓缩38 K的蛋白,穿滤. 浓缩液，A3.5 (
280 nm)滤出液 A2.7 (280 nm).是质量不好? 以前也出现过，换一个就好了。这次试了
一盒filter都穿滤. |
|
y****y
发帖数: 275 | 9 我们需要买一个continuous centrifuge， 大型的有commercialized的，但是我们需要
体积和容量小的，可以fit进我们实验室和试验台上的。不知道哪位兄弟有这个经验或
者信息的，google了半天没找到合适的。多谢多谢！ |
|
y****y
发帖数: 275 | 10 我们需要买一个continuous centrifuge， 大型的有commercialized的，但是我们需要
体积和容量小的，可以fit进我们实验室和试验台上的。不知道哪位兄弟有这个经验或
者信息的，google了半天没找到合适的。多谢多谢！ |
|
y****y
发帖数: 275 | 11 我们需要买一个continuous centrifuge， 大型的有commercialized的，但是我们需要
体积和容量小的，可以fit进我们实验室和试验台上的。不知道哪位兄弟有这个经验或
者信息的，google了半天没找到合适的。多谢多谢！ |
|
c*******n
发帖数: 1648 | 12 I think centrifuging used to be a popular method for determiniing of M of
macromolecules.
A lot of old papers must have covered what you want to know. I have basic
principles of this method in a Chinese version Polymer Physics book, but no
details. For dilute solution, partial volume will not be very different from
volume in pure solvent. Maybe you can use volume for pure solvent:) |
|
y****y
发帖数: 275 | 13 我们需要买一个continuous centrifuge， 大型的有commercialized的，但是我们需要
体积和容量小的，可以fit进我们实验室和试验台上的。不知道哪位兄弟有这个经验或
者信息的，google了半天没找到合适的。多谢多谢！ |
|
y****y
发帖数: 275 | 14 我们需要买一个continuous centrifuge， 大型的有commercialized的，但是我们需要
体积和容量小的，可以fit进我们实验室和试验台上的。不知道哪位兄弟有这个经验或
者信息的，google了半天没找到合适的。多谢多谢！ |
|
g*********5
发帖数: 2533 | 15 Isolation of nuclei for the co-IP and whole ChIP protocols is based on the
methods of Gendrel et al. (2002, 2005), Johnson et al. (2002), and Nelson et
al. (2006).
METHOD
For extraction and co-IP of nuclear proteins, see Steps 1-39 (Fig. 1). For
the ChIP procedure, see Steps 40-69 (Fig. 2).
Figure 1.
View larger version:
In this page
In a new window
Figure 1.
Flowchart for the timeline and organization for co-IP of nuclear proteins
from Arabidopsis seedlings.
Figure 2.
View larger versio... 阅读全帖 |
|
h**********r
发帖数: 671 | 16 我以前在国内时候提细菌基因组用的方法，貌似对酵母也work,我以前就提过一次酵母
，确实work的很好，还记得gel上的照片贼亮贼亮的。那时候年轻啊！问过别人，说
lysozyme对yeast也能work.
Extraction of DNA from Bacteria and Yeasts
(This protocol was developed by Housepainter on February 09, 2010)
1.Inoculate 5 ml of medium with a single colony of transformed bacteria or
yeasts.
2.Pour 1.0 ml of the culture into a tube. Centrifuge at maximum speed for 30
seconds at room temperature. Remove the supernatant.
3.Resuspend the bacterial pellet in 1.0 ml 0.85% NaCl by vigorous vortexi... 阅读全帖 |
|
h**********r
发帖数: 671 | 17 我在实验室用的Thermo，貌似work的不是很好。
Yeast DNA Extraction
(The protocol was modified by housepainter on December 28, 2009 according to
manufacture’s instructions)
Yeast DNA Extraction Kit, Thermo Number Description: 78870
Storage: Store at 4°C; if precipitant has formed, gently warm DNA Releasing
Reagents A and B at 37°C for 1-5 minutes.
Kit Contents:
Y-PER Reagent, 25 ml
DNA Releasing Reagent A, 20 ml
DNA Releasing Reagent B, 20 ml
Protein Removal Reagent, 10 ml
1. Pellet a 10 ml S. cerevisiae culture gr... 阅读全帖 |
|
n******7
发帖数: 5678 | 18 China Joins the Axis of Evil
By BRET STEPHENS
Last month, U.S. nuclear scientist Siegfried Hecker paid his fourth visit to
North Korea, where he was granted a tour of some of the hermit kingdom's
nuclear facilities. Think WikiLeaks is bad? Compared to what the former
director of the Los Alamos lab saw, it's nothing.
Mr. Hecker was given a tour of a construction site where Pyongyang intends
to build a 100-megawatt reactor. Next he was taken to a uranium enrichment
facility. "The first look throug... 阅读全帖 |
|
h**f
发帖数: 52 | 19 显然是在压迫党core在访美的时候多带点礼物。
http://online.wsj.com/article/SB10001424052748704156304576003124111945808.html
China Joins the Axis of Evil
Pyongyang's nuclear program would have been impossible without Beijing.
Last month, U.S. nuclear scientist Siegfried Hecker paid his fourth visit to
North Korea, where he was granted a tour of some of the hermit kingdom's
nuclear facilities. Think WikiLeaks is bad? Compared to what the former
director of the Los Alamos lab saw, it's nothing.
Mr. Hecker was given a t... 阅读全帖 |
|
l*****7
发帖数: 8463 | 20 http://www.gwu.edu/~nsarchiv/NSAEBB/NSAEBB80/
home | about | documents | news | publications | FOIA | research |
internships | search | donate | mailing list
Iraq and Weapons of Mass Destruction
National Security Archive Electronic Briefing Book No. 80
Updated - February 11, 2004
Edited by Jeffrey Richelson
Originally posted December 20, 2002
Previously updated February 26, 2003
Documents - Press release - Further reading
Between Iraq's invasion of Kuwait in August 1990, and the commencement of... 阅读全帖 |
|
l****z
发帖数: 29846 | 21 Tehran can continue to enrich uranium at 10,000 working centrifuges.
Updated Nov. 24, 2013 10:18 p.m. ET
President Obama is hailing a weekend accord that he says has "halted the
progress of the Iranian nuclear program," and we devoutly wish this were
true. The reality is that the agreement in Geneva with five Western nations
takes Iran a giant step closer to becoming a de facto nuclear power.
Start with the fact that this "interim" accord fails to meet the terms of
several United Nations resolut... 阅读全帖 |
|
c***y
发帖数: 286 | 22 let's read the full text here. The title is totally off the hook:
Last month, U.S. nuclear scientist Siegfried Hecker paid his fourth visit to
North Korea, where he was granted a tour of some of the hermit kingdom's
nuclear facilities. Think WikiLeaks is bad? Compared to what the former
director of the Los Alamos lab saw, it's nothing.
Mr. Hecker was given a tour of a construction site where Pyongyang intends
to build a 100-megawatt reactor. Next he was taken to a uranium enrichment
facility. "T... 阅读全帖 |
|
h**********r
发帖数: 671 | 23 以前做Neurospora的knockout的时候，看到过yeast方面的protocol。感觉他们挺严谨
的。但我不保证work。摘自：
http://www.dartmouth.edu/~neurosporagenome/Projects_files/Proje
Prepare yeast DNA with the Gentra yeast DNA kit:
(the following is slightly modified from their protocol:
http://www.gentra.com/pdf/01160.pdf)
the solutions are available from Gentra as Puregene kit D-6000A or
separately; if you have their Puregene solutions for Neurospora DNA preps,
you only need to order the Cell Suspension Solution (D-6001) and Lytic
Enzyme Sol... 阅读全帖 |
|
b*********b
发帖数: 64 | 24 I use QIAGEN Plasmid Midi kit. 100ml culture could give about 30-50ug. I
tried Qiagen's large construct kit.But in my hand, the midi kit is much much
better than the large construct kit, and also takes much less time.The
following is the protocol I got from Qiagen.
User-Developed Protocol:
Isolation of BAC DNA using the QIAGEN® Plasmid Midi Kit
This procedure has been adapted by customers from the QIAGEN® Plasmid
Midi Kit Protocol.It has not been thoroughly tested and optimized by QIAG... 阅读全帖 |
|
J**********7
发帖数: 2619 | 25 新手常犯的错误就是一开始发力过猛.
shimano的刹车对这种错误更tolerant.
Casting brakes are the thing that prevents birdsnest when casting, every
brand has the variations and combinations of brakes but there are
essentially 2 main types, magnetic and centrifugal. As a general rule
centrifugal brakes are better at controlling the spool at the beginning of
the cast where as magnetic brakes are better at controlling the latter part
of the cast. What does that mean to an angler? It means centrifugally
controlled reels are more ... 阅读全帖 |
|
发帖数: 1 | 26 这个我知道，拨出来就是开了，拨进去卡住就是关了。但我不明白的是，这个塑料片跟
centrifugal有什么关系。它不是在spool上的，而是在sideplate上的，所以casting的
时候，塑料片是不动的，那何来centrifugal force？Centrifugal brake比generic
magnetic brake有优势的地方，就在于它是在spool转得越快的时候，利用离心力，提
供越大的摩擦阻力，当spool转速下降的时候，离心力跟随下降，阻力也就下降了，这
样在理论上实现最大抛投距离，而且不会backlash。而generic magnetic brake提供的
阻力是不随着spool转速而变化的，提供的是线性的不变阻力，所以在casting开始的时
候可能不够容易出现backlash，在后面的时候又太大了，影响抛投距离。
所以我真不觉得这几个塑料片是centrifugal brake，只能算是额外的固定阻尼。 |
|
发帖数: 1 | 27 这个我知道，拨出来就是开了，拨进去卡住就是关了。但我不明白的是，这个塑料片跟
centrifugal有什么关系。它不是在spool上的，而是在sideplate上的，所以casting的
时候，塑料片是不动的，那何来centrifugal force？Centrifugal brake比generic
magnetic brake有优势的地方，就在于它是在spool转得越快的时候，利用离心力，提
供越大的摩擦阻力，当spool转速下降的时候，离心力跟随下降，阻力也就下降了，这
样在理论上实现最大抛投距离，而且不会backlash。而generic magnetic brake提供的
阻力是不随着spool转速而变化的，提供的是线性的不变阻力，所以在casting开始的时
候可能不够容易出现backlash，在后面的时候又太大了，影响抛投距离。
所以我真不觉得这几个塑料片是centrifugal brake，只能算是额外的固定阻尼。 |
|
S******e
发帖数: 393 | 28 将S1 (cytosolic protein) 和 S3 (nuclear soluble protein?) 去掉后，剩下的
pellet再用0.1%的Triton extract, 收集到的上清暂称为X，想知道X是些什么蛋白？
Chromatin associated protein?
方法如下：
1. Cells were extracted with Buffer A (10mM Hepes pH7.9, 10mM KCl, 1.5mM
MgCl2, 0.34mM Sucrose, 10% Glycerol, 1mM DTT plus protease inhibitors)
containing 0.1% Triton for 5 min on ice, centrifuge 1300g X5 min, discard
supernatant (S1), wash once with BufferA and discard supernatant.
2. Extract cell pellet with Buffer B (3mM EDTA, 0.2mM EGTA, 1mM... 阅读全帖 |
|
C*I
发帖数: 4736 | 29 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 30 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 31 Published: 30 October 2013
Isolation and characterization of a bat SARS-like coronavirus that uses the
ACE2 receptor
Xing-Yi Ge, Jia-Lu Li, Xing-Lou Yang, Aleksei A. Chmura, Guangjian Zhu,
Jonathan H. Epstein, Jonna K. Mazet, Ben Hu, Wei Zhang, Cheng Peng, Yu-Ji
Zhang, Chu-Ming Luo, Bing Tan, Ning Wang, Yan Zhu, Gary Crameri, Shu-Yi
Zhang, Lin-Fa Wang, Peter Daszak & Zheng-Li Shi
Nature volume 503, pages535–538(2013)Cite this article
Abstract
The 2002–3 pandemic caused by severe acute respirator... 阅读全帖 |
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l****z
发帖数: 29846 | 32 Vive La France on Iran
The French save the West from a very bad nuclear deal with Iran.
Updated Nov. 10, 2013 5:23 p.m. ET
We never thought we'd say this, but thank heaven for French foreign-policy
exceptionalism. At least for the time being, François Hollande's
Socialist government has saved the West from a deal that would all but
guarantee that Iran becomes a nuclear power.
While the negotiating details still aren't fully known, the French made
clear Saturday that they objected to a nucle... 阅读全帖 |
|
d**********0
发帖数: 13081 | 33 http://time.com/3770850/iran-deal-explainer/
So what does the framework agreement say?
According to the framework agreement, Iran agreed to cut by two-thirds its
supply of centrifuges, from roughly 19,000 to about 6,000, and retain only
its earliest generation centrifuges. It said it would keep continuing
enrichment far below levels necessary for a nuclear weapon and also agreed
to reduce its stockpile of enriched uranium by 97%.
But exactly how it plans to scrap its extra centrifuges and enrich... 阅读全帖 |
|
f*******0
发帖数: 62 | 34 有幸得到一个AP position， 现需要估算个 startup package。 因为我主要是用E.
Coli express and purify membrane proteins，特向有经验前辈们请教几个主要用到
的仪器大概价格 （有个区间就行）。多谢多谢！
1. High performance liquid chromatography
2. 1L bacteria incubation shaker
3. OD spectrometer (for bacteria concentration measurement)
4. Allegra type centrifuge (for protein solution centrifuge／concentrate)
5. Bacteria cell pelleting, harvesting centrifuge
6. ultracentrifuge
7. Benchtop microcentrifuge
8. mechanical bacterial cell breaker
9. UV absorption spectr... 阅读全帖 |
|
W********s
发帖数: 1705 | 35 用过BC就不想碰Spinning了。用饵的方式种类大增！我目前只有一条Spinning Setup，
用来作Drop Shot。
一般苦练二到四小时就有小成。是不停的练，而不是半小时Here And There。用胶布贴
防止过多炒粉是个好主意。轮子不能买太便宜的，100刀以上的。先玩重点的，1/2盎司
，什么Mag或是Centrifugal都上到最紧，Spool Tension调节到饵慢慢落地而Spool上没
有虚线。那就开始练吧，小臂手腕力量居多，拇指可以轻按Spool当刹车。拇指控线很
重要，要Become Second Nature需要时间。不能多想动作，你脑子有想动作就是没练成
，就会炒粉。张三丰问无忌，还记得几成，无忌说全忘了，那就成了！这个练熟之后，
开始松Spool Tension，或是松Mag，Centrifugal，One Step A Time。甩出去立马有
缠线现象，Mag，Centrifugal松了，后面有缠线就Spool Tension松了。最好是抛出去
落水之后，Spool上有一点虚线要扯二下，（不是坏事，在扯二下的时候有时就中鱼了
）这就是这个轮子用这... 阅读全帖 |
|
w******a
发帖数: 1527 | 36 Check this one:
Reagents
* TRIzol from Invitrogen #15596-108.
* Mini RNeasy plus kit from Qiagen #74134.
* Phase lock gel from Eppendorf #2302830.
* Keep TRIzol at 4C.
* Use RNase-free tubes and tips for all steps.
* Prepare 70% ethanol using DEPC-H2O and keep it at -20C.
RNeasy plus kit contains a genomic DNA elimination column. It can replace
the DNase I digestion step of regular RNeasy kit.
1. Add 1.2 ml TRIzol reagent to cells/tissues (5~10X 106 cells/ml or 50~100
mg ... 阅读全帖 |
|
f*******0
发帖数: 62 | 37 【 以下文字转载自 Faculty 讨论区 】
发信人: fishman80 (最爱鱼儿), 信区: Faculty
标 题: 请教几个用来产生 membrane proteins设备的价格
发信站: BBS 未名空间站 (Mon Feb 25 13:44:06 2013, 美东)
有幸得到一个AP position， 现需要估算个 startup package。 因为我主要是用E.
Coli express and purify membrane proteins，特向有经验前辈们请教几个主要用到
的仪器大概价格 （有个区间就行）。多谢多谢！
1. High performance liquid chromatography
2. 1L bacteria incubation shaker
3. OD spectrometer (for bacteria concentration measurement)
4. Allegra type centrifuge (for protein solution centrifuge／concentrate)
5. Bacteria ce... 阅读全帖 |
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v********a
发帖数: 646 | 38 1) Research Scientist, Antibody Purification (Biochemistry / Antibody)
2) Research Scientist, Reagent Printing (Biochemistry / Printer)
3) Research Scientist, Assay Development (Biochemistry / Red Blood Cell)
1) 15099 - Research Scientist, Antibody Purification (Biochemistry /
Antibody)
Norcross, GA, US
Employment Type: Full-Time
This position is not eligible for sponsorship for work authorization by
Immucor, Inc. Therefore, if you will require sponsorship from us for work
author... 阅读全帖 |
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W******r
发帖数: 789 | 39 By Mark A Ament
If you only make one improvement to your diet this year, I hope it is to
replace refined sugar with raw food sweeteners. If you do, I can guarantee
you that smoother digestion, more relaxed muscles, more peaceful emotions
and increased mental clarity are soon to follow.
Before looking at the negative health consequences of eating refined sugar,
let's have a quick look at how it's made. This will give you some insight
into why it affects the body the way it does. The majority of s... 阅读全帖 |
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w********2
发帖数: 632 | 41 An air-cooled rotary that powered a number of successful World War I
aircraft
History
The rotary engine is unique in that, unlike fixed radial engines, it has a
stationary crankshaft bolted to the firewall, with everything else (
cylinders, crankcase, propeller) all rotating. The rotary in its original
form is extinct, with the term now being used to describe "Wankel" rotary
combustion engines such as those built by Mazda. Though automotive and
motorcycle rotaries were built in the United State... 阅读全帖 |
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b*****h
发帖数: 3386 | 42 TEHRAN, April 9 (Reuters) - Iran is now running 7,000 uranium enrichment
centrifuges, a senior official said on Thursday, an announcement likely to
increase Western concerns about the Islamic Republic's disputed nuclear
plans.
Gholamreza Aghazadeh, head of Iran's Atomic Energy Organisation, also said
it had obtained the technology to produce more "accurate" centrifuges, which
are used to enrich uranium.
Referring to the inauguration of a nuclear fuel production plant by
President Mahmoud Ahmadin |
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j*****y
发帖数: 121 | 43 Novo Nordisk R&D Center China
Mini-pilot – Senior/Principal Scientist in DSP
Zhongguancun Life Science Park, Beijing
Novo Nordisk is a focused healthcare company and a world leader in diabetes
care. Novo Nordisk China R&D (NNRCC) is an integrated part of Novo Nordisk R
&D organization. Novo Nordisk wants to conduct pharmaceutical research
activities in China and expand its network to the scientific community. For
this purpose a new position is open for a dedicated and motivated Senior
Scientist/... 阅读全帖 |
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j*****y
发帖数: 121 | 44 Novo Nordisk R&D Center China
Mini-pilot – Senior/Principal Scientist in DSP
Zhongguancun Life Science Park, Beijing
Novo Nordisk is a focused healthcare company and a world leader in diabetes
care. Novo Nordisk China R&D (NNRCC) is an integrated part of Novo Nordisk R
&D organization. Novo Nordisk wants to conduct pharmaceutical research
activities in China and expand its network to the scientific community. For
this purpose a new position is open for a dedicated and motivated Senior
Scientist/... 阅读全帖 |
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T**r
发帖数: 7016 | 45 通过对比离心刹车centrifugal brake system和磁性刹车magnetic brake system，我觉得centrifugal break system从原理
上来说更合理一点。换句话说，如果其它条件都相同，用离心刹的轮比用磁性刹的轮投
得要远一些。
原因简单地说：在投掷的时候，离心刹产生的阻力是暂时的、即时的，而磁性刹的阻力
是持续的。
更近一步地解释：炸线一般出现在两个时间段，一是投掷起始段，二是落水时。落水炸
线很好解决，用大一点轴压力就行，熟练以后一般是不用轴压力而在lure落水时用大拇
指来停住spool。
投掷起始段的炸线是因为出线速度比lure飞行速度快（因为风阻力或其它的原因），这
种情况一般是在投出后的一小段时间（可能占整个投掷时间的五分之一），这段时间有
初始的加速度，如果lure稍有受阻，就会炸线。离心和磁性刹车系统就是为了减小这个
加速度。离心刹车是利用摩擦产生阻力：摩擦片由于离心力的作用向外伸出，摩擦到固
定的轮圈。磁性刹车是利用磁性产生阻力：通过调整磁铁片和spool的距离，磁铁片会
对spool的转速有限制作用。这两种刹车的不同之处在于 |
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W*******s
发帖数: 18705 | 46 最近对我以前的渔具玩得非常厌烦了，我也没多闲钱烧日货，在Ebay上倒腾卖出买进，
连BassProShop，TacklewareHouse的零买，搞了6条杆子，六个轮子，玩的是Down
Grade,基本倒贴了没多少钱，把装备翻新了一下。现在主打轮子是Quantum，Daiwa，杆
子是黑胡萝卜杆--我拿过得最轻的杆子（橘红色的也差不多，不能消化这颜色），
Quantum Smoke。卖好几年前的高级轮子，Daiwa TDZ,再把新轮子解体洗涤重新上油/腻
，我发现，尼玛所有的轮子内部结构都几乎一样，任何Low Profile Baitcast轮子我基
本上不用看图就能拆装。目前美国市场上卖的淡水上手轮分中国，韩国，日本，美国制
造。中国，韩国制造的牌子有Abu，Quantum，Okuma，Lews，BassProShop，Pfluger，
Pinnacle，某些Shimano，Daiwa低端轮子，等等占大部分的市场。这些轮子的品牌多为
个幌子，轮子基本上都是“保密”的相同中国韩国工厂生产的，很多零件都是通用的，
大同小异。打开Lews Tournament Pro，Quantum Sm... 阅读全帖 |
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W*******s
发帖数: 18705 | 47 这个才算是比较正宗的BC Surf轮子，运河老臣多用这类。我的Daiwa Luna好像有点吃
力，把它当作淡水Swimbait 轮子把，非常合适的。
Rocket应该是最好的BC远投轮之一，轮速还是慢的，我加了个Power Handle，希望能摇
快点。
ABU Sweden sometimes release reproduction models of its classic models, and
these ABU CS Rocket reels are released only for Japan market in 2015.
The release is for Blue Grey color. Brass frame holds mechanicals solid, and
the chrome plating on it shines great.
These reels are the reproduction of classic 5500 and 6500CS Rocket with
instant anti-reverse system, originall... 阅读全帖 |
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发帖数: 1 | 48 版主屡次提到Daiwa的杆轮系列，个人也觉得Daiwa的产品性价比非常高，尤其Tatula，
确实是workhorse reel里面的佼佼者。我有一个Exceler, 一个Tatula 100, 一个
Tatula CT，一个Tatula SV, 和一个Zillion。杆子方面有一个Tatula的杆子。这里就
比较一下吧，给感兴趣的钓友一些参考意见。
Exceler系列是属于低端系列了，比Aird系列要高一些，毕竟是金属框架，但又比中端
主力产品Tatula要低，基本上是downgraded version的Lexa 100。但跟Lexa 100系列一
样，现在基本停产了，市面上不多，这里提一下主要是因为个人觉得cast方面非常的好
用，虽然抛投距离不是最远的但也不错了，而且极少backlash，即使把brake调在
minimum的位置，几乎不用thumb。这点比较适合刚开始用BC reel的朋友。我觉得是因
为spool跟line guide的距离的原因，比一般的reel要远，所以线cast出去的时候，跟
line guide的摩擦阻力会小些。用的是Brake是普通的Magforc... 阅读全帖 |
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发帖数: 1 | 49 当年Kastking名气还不大的时候，就买过一个Royal Legend Baitcaster尝试，刚开箱
时还行，但很快就发现问题多多。
1.像你说的，spool和frame之间的gap太大，这个gap本来应该是sideplate填上的，但
加工tolerance实在不怎样，所以这个gap在spool太满的时候绕线，而且很容易渗水进
去，我经常打开sideplate发现里面全是水。
2.它号称是centrifugal和magnetic dual braking，但我到现在也没搞明白它的
centrifugal brake的工作原理，实用中也很不稳定，噪音很大，加油也没用，所以我
基本就只用它的magnetic brake了
3.gear的加工精度实在不咋地，用了不到一两个月，就有那种明显的gear grinding的
感觉了，跟bearing没关系，想消除这种“geary feel”，只有更换pinion gear。所有
的reel用久了都不可避免的会出现这种“geary feel”，但质量好的象Shimano, Daiwa
的轮子，要很久以后才会变得明显。
4.用了好几个carbo... 阅读全帖 |
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发帖数: 1 | 50 当年Kastking名气还不大的时候，就买过一个Royal Legend Baitcaster尝试，刚开箱
时还行，但很快就发现问题多多。
1.像你说的，spool和frame之间的gap太大，这个gap本来应该是sideplate填上的，但
加工tolerance实在不怎样，所以这个gap在spool太满的时候绕线，而且很容易渗水进
去，我经常打开sideplate发现里面全是水。
2.它号称是centrifugal和magnetic dual braking，但我到现在也没搞明白它的
centrifugal brake的工作原理，实用中也很不稳定，噪音很大，加油也没用，所以我
基本就只用它的magnetic brake了
3.gear的加工精度实在不咋地，用了不到一两个月，就有那种明显的gear grinding的
感觉了，跟bearing没关系，想消除这种“geary feel”，只有更换pinion gear。所有
的reel用久了都不可避免的会出现这种“geary feel”，但质量好的象Shimano, Daiwa
的轮子，要很久以后才会变得明显。
4.用了好几个carbo... 阅读全帖 |
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