W*****o
发帖数: 1780 | |
b****r
发帖数: 17995 | 2 两边都会，所以无论5‘帽或者3’poly A有问题，RNA都会不稳定
从中间断应该不多，反正没听过 |
s****9
发帖数: 932 | 3 3' 段更容易降解，如果做realtime，用random primer，最好把引物设计在5段，这样
增加efficiency，也增加consistency
【在 W*****o 的大作中提到】
: 一直没搞清楚
|
c********b
发帖数: 363 | 4 植物里面经常是中间断开，被小RNA guide cleavage。
【在 b****r 的大作中提到】
: 两边都会，所以无论5‘帽或者3’poly A有问题，RNA都会不稳定
: 从中间断应该不多，反正没听过
|
i*********0
发帖数: 915 | 5 会不会比较大的mRNA或者其他的RNA， 中间切一刀，然后在从两端开始切，这个过程会
不会快一点。 |
l**********1
发帖数: 5204 | 6 Plant
There is reason to think that mRNA degradation sometimes starts with the
action of ribonuclease III, an enzyme specific for duplex RNA, which could
cleave in stem-loop structures and create sites for exonucleolytic attack.
RNase III is actually involved in the maturation of certain phage mRNAs as
they undergo posttranscriptional processing, but this involvement is not
known to occur with bacterial mRNAs.
PMID 17693527
Watkins KP et al.
A ribonuclease III domain protein functions in group II intron splicing in
maize chloroplasts. (2007).
Plant Cell. 19: 2606-23.
http://www.ncbi.nlm.nih.gov/pubmed/17693527
Bacterial and Animal:
Mechanism of degradation -
It is known that degradation starts from the 5' end, which is important
because translation also starts from the 5' end. If degradation were to
start from the 3' end, a ribosome starting from a 5' end might never reach
an intact 3' end.
http://www.pearsonhighered.com/mathews/ch26/c26mt.htm |
