m******5
发帖数: 1383 | 1 1，哪些现有的EGFP reporter mouse strain可以light up Sinus atria node
这个我印象中是没有的
2, 有人in-vitro culture过 mouse sinus atria node cell么？
我印象中只有人culture过rat的 |
s**********y
发帖数: 144 | 2 I think there is HCN4-EGFP mouse line, which SA node is green. |
m******5
发帖数: 1383 | 3 1，哪些现有的EGFP reporter mouse strain可以light up Sinus atria node
这个我印象中是没有的
2, 有人in-vitro culture过 mouse sinus atria node cell么？
我印象中只有人culture过rat的 |
s**********y
发帖数: 144 | 4 I think there is HCN4-EGFP mouse line, which SA node is green. |
m******5
发帖数: 1383 | 5 I couldn't find the publication , could you attach a link? thanks!!
【在 s**********y 的大作中提到】
: I think there is HCN4-EGFP mouse line, which SA node is green.
|
r*******y
发帖数: 48 | 6 look at two publications in genesis and dev cell from Ivan Moskowitz at
university of chicago. |
s**********y
发帖数: 144 | 7 I found this one :HCN4-H2B-EGFP knock-in mice
used in this paper from Sylvia Evans lab
Islet 1 is Expressed in Distinct Cardiovascular Lineages, Including
Pacemaker and Coronary Vascular Cells
Not sure if it is what u r looking for.
and there is a transgenic HCN4-GFP line
http://www.informatics.jax.org/javawi2/servlet/WIFetch?page=all |
m******5
发帖数: 1383 | 8 That is also what I have found, but looks like they are not going to release
this 'elsewhere' paper anytime soon.
【在 s**********y 的大作中提到】
: I found this one :HCN4-H2B-EGFP knock-in mice
: used in this paper from Sylvia Evans lab
: Islet 1 is Expressed in Distinct Cardiovascular Lineages, Including
: Pacemaker and Coronary Vascular Cells
: Not sure if it is what u r looking for.
: and there is a transgenic HCN4-GFP line
: http://www.informatics.jax.org/javawi2/servlet/WIFetch?page=all
|
m******5
发帖数: 1383 | 9 I don't think they have SAN EGFP
they use mink-EGFP/LacZ which is a whole CCS reporter
【在 r*******y 的大作中提到】
: look at two publications in genesis and dev cell from Ivan Moskowitz at
: university of chicago.
|
s**********y
发帖数: 144 | 10 I think you can contact the lab directly if this is the mouse that you want.
I have seen another paper using this mouse also.They for sure have them.
In my previous lab, we had HCN4-GFP mice but I don't know where my PI got
them. I left there so I cant get the information. |
s**********y
发帖数: 144 | 11 Are you looking for San only? It seems to me, hcn4, tbx3 also mark other
conduction system in addition to the San. There seems not to be a very very
clean one that only marks San. I could be wrong. |
m******5
发帖数: 1383 | 12 Thank you! Neither do I know if there is any reporter line mark exclusively
the SAN , I am just thinking about if it is possible to make one.
very
【在 s**********y 的大作中提到】
: Are you looking for San only? It seems to me, hcn4, tbx3 also mark other
: conduction system in addition to the San. There seems not to be a very very
: clean one that only marks San. I could be wrong.
|
