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Biology版 - Have anyone see bubbles in nuclei?
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话题: nuclei话题: bubbles话题: gfp话题: spots话题: bright
进入Biology版参与讨论
1 (共1页)
d****e
发帖数: 13
1
What is this? I transfected Hela cells with p27 fused to GFP by calcium
phosphate method. The GFP fluorescence localized to nuclei but there a few
bright spots in the nuclei corresponding to the bubbles under bright fields.
Is this normal? are those spots auto fluorescent?
G*******d
发帖数: 359
2
I saw it before in my immuno staining
1 (共1页)
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仇子龙的申明中说他们实验室用韩春雨的NgAgo在293细胞中看到测除了293,什么细胞容易做plasmid transfection?
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求助Hela transfectionstable cell line 筛选问题
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相关话题的讨论汇总
话题: nuclei话题: bubbles话题: gfp话题: spots话题: bright