w*e
发帖数: 740 | 1 有正在建2A系统的的么？
我想确认一下我的T2A,P2A的DNA序列是否正确，
网上文章的，还有addgene上的序列都不大一样
谢谢 |
a****d
发帖数: 1919 | 2 There are several 2A sequence.
One way to check is to google the 2A sequence, you may find the papers using
that sequence. |
w*e
发帖数: 740 | 3 谢谢
我找到了几个文章，也从addgene上搜索到了
但序列比对后，都不一样，
我想用的是T2A,P2A
using
【在 a****d 的大作中提到】
: There are several 2A sequence.
: One way to check is to google the 2A sequence, you may find the papers using
: that sequence.
|
a****d
发帖数: 1919 | 4 For your reference, I use this lentivector from System Bio, pCDH-T2A
http://www.systembio.com/downloads/Manual_pCDH_Vector_v5.pdf
Page 10
The T2A sequence for this vector:
gag ggc aga gga agt ctt cta aca tgc ggt gac gtg gag gag aat ccc ggc cct |
w*e
发帖数: 740 | 5 非常感谢
【在 a****d 的大作中提到】
: For your reference, I use this lentivector from System Bio, pCDH-T2A
: http://www.systembio.com/downloads/Manual_pCDH_Vector_v5.pdf
: Page 10
: The T2A sequence for this vector:
: gag ggc aga gga agt ctt cta aca tgc ggt gac gtg gag gag aat ccc ggc cct
|
