z*****g
发帖数: 306 | 1 Renzhen qingjiao wenti. Duo xie!
I have two genes: A and B. They do not match each other based on DNA
sequence. I am pretty sure that A and B possess the same/similar function. I
know the peptide sequence in A gene correspending to the function. How to
find out the its counterpart in B gene? Is there a free online program for
this purpose?
Duo xie!! |
K**4
发帖数: 1015 | 2 blast
I
【在 z*****g 的大作中提到】
: Renzhen qingjiao wenti. Duo xie!
: I have two genes: A and B. They do not match each other based on DNA
: sequence. I am pretty sure that A and B possess the same/similar function. I
: know the peptide sequence in A gene correspending to the function. How to
: find out the its counterpart in B gene? Is there a free online program for
: this purpose?
: Duo xie!!
|
s***m
发帖数: 6197 | 3 Try protein structure prediction? Then structural alignment.
Assuming the structure and function relationship.
I
【在 z*****g 的大作中提到】
: Renzhen qingjiao wenti. Duo xie!
: I have two genes: A and B. They do not match each other based on DNA
: sequence. I am pretty sure that A and B possess the same/similar function. I
: know the peptide sequence in A gene correspending to the function. How to
: find out the its counterpart in B gene? Is there a free online program for
: this purpose?
: Duo xie!!
|
z*****g
发帖数: 306 | 4 Yes. I want to figure out the same functional domain in gene B as that in A.
I tried Blast to align the two gene, but Blast only shows the sequence
similarity, without functional information.
I appreciate any help! Duo xie!! |
l*********s
发帖数: 5409 | 5 if you are sure about B's function, you can go ahead to mutation analysis to
pin down the domain. |
K**4
发帖数: 1015 | 6 try CDD or pfam.
A.
【在 z*****g 的大作中提到】
: Yes. I want to figure out the same functional domain in gene B as that in A.
: I tried Blast to align the two gene, but Blast only shows the sequence
: similarity, without functional information.
: I appreciate any help! Duo xie!!
|
m***c
发帖数: 177 | 7 you can look at interpro and find out the domain information for both A and
B. You may see similar domains. |
