t*********1
发帖数: 418 | 1 I am trying to clone a promoter sequence to a Vector. Now I have a question
on designing primers. I want to add a restriction enzyme at the end of each
primer, and I also heard that it is better to add a few bp around the
restriction enzyme for it to be cleaved. Does anyone know where I can find
such information on which nucleotide to be added to different restriction
enzyme?
(I read New England catalog book and did not find such information.)
Thank you! | b********n
发帖数: 22 | 2 The old catalog has the information, but it is removed somehow.
Usually, just add GGTGGT to the 5' of the restriction site.
question
each
【在 t*********1 的大作中提到】
: I am trying to clone a promoter sequence to a Vector. Now I have a question
: on designing primers. I want to add a restriction enzyme at the end of each
: primer, and I also heard that it is better to add a few bp around the
: restriction enzyme for it to be cleaved. Does anyone know where I can find
: such information on which nucleotide to be added to different restriction
: enzyme?
: (I read New England catalog book and did not find such information.)
: Thank you!
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