z*h
发帖数: 773 | 1 Brief Introduction of Biofuels Lab at Virginia Tech
“Imagination, Invention, innovation --- Replacing Crude Oil by Sugars
through Cascade Enzyme Factories”. Website: http://sugarcar.com
The mission of the Biofuels Laboratory led by associate professor Yi-Heng
Percival Zhang at Virginia Polytechnic Institute and State University (USA)
is to solve key sustainability challenges – What will replace crude oil?
How and when? How to feed the world in future? Dr. Zhang is a world
recognized young bioc... 阅读全帖 |
|
z*h
发帖数: 773 | 2 Brief Introduction of Biofuels Lab at Virginia Tech
“Imagination, Invention, innovation --- Replacing Crude Oil by Sugars
through Cascade Enzyme Factories”. Website: http://sugarcar.com
The mission of the Biofuels Laboratory led by associate professor Yi-Heng
Percival Zhang at Virginia Polytechnic Institute and State University (USA)
is to solve key sustainability challenges – What will replace crude oil?
How and when? How to feed the world in future? Dr. Zhang is a world
recognized young bioc... 阅读全帖 |
|
t*********1
发帖数: 418 | 3 I am trying to clone a promoter sequence to a Vector. Now I have a question
on designing primers. I want to add a restriction enzyme at the end of each
primer, and I also heard that it is better to add a few bp around the
restriction enzyme for it to be cleaved. Does anyone know where I can find
such information on which nucleotide to be added to different restriction
enzyme?
(I read New England catalog book and did not find such information.)
Thank you! |
|
s********p
发帖数: 103 | 4 新买的房子前屋主有只猫，搬进去前要做carpet cleaning,但可以选择要不要做enzyme
treatment,有的contractor说看地毯情况，不一定需要。不知是不是呢？如何知道要
不要做enzyme treatment? 谢谢 ！ |
|
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P**K
发帖数: 80 | 6 Got email for the postdoctoral positions. FYI
The Enzyme Function Initiative (EFI) has been established with a Glue Grant
from
NIGMS/NIH (U54GM093342). The EFI’s goal is to develop a general
sequence/structure-based
strategy for facilitating discovery of in vitro enzymatic and in vivo
metabolic/physiological
functions of unknown enzymes discovered in genome projects, a crucial
limitation in
genomic
biology. This goal will be accomplished by integrating bioinformatics,
structural
biology, and
com |
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h*****g
发帖数: 23 | 7 谢谢楼上两位朋友，我是半路过来想利用一下non-heme iron enzyme的，不是专门做这
个，所以想请教有经验的朋友关于non-hemeiron enzyme的基础问题 |
|
h*****g
发帖数: 23 | 8 你好！我想请教一下,基于目前的研究,什么样的non-heme iron enzyme的complex是容
易被孤立出来,稳定性好，尺寸不要太大，并且研究显示它是催化氧化活性特别好的,比
方说氧化CH4到CH3OH或者苯到苯酚,等等.
如果没有合适的non-heme iron enzyme，那么heme的也可以。
先多谢了！！ |
|
k***i
发帖数: 662 | 9 August 23, 2007
Also appeared in print Aug. 27, 2007, p. 12
ACS Meeting News
Unique Enzyme Could Lead To Novel Antibiotics
Promiscuous transglutaminase in andrimid biosynthesis might yield improved analogs
Amanda Yarnell
Recent efforts to characterize an unusual catalyst in the enzymatic assembly line dedicated to andrimid production could lead to new, improved analogs of this antibiotic, researchers reported at the ACS national meeting in Boston this week.
AN ENZYME OF INTEREST AdmF creates |
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j******0
发帖数: 307 | 10 rt，求一个AmorePacific Treatment Enzyme Peel, 0.2 oz，$4/each,label ready
请站内联系！ |
|
g***l
发帖数: 18555 | 11 不喝牛奶就是了，lactase enzyme要提前吃才管用，那么贵，哪吃得起。我现在喝半杯
2%的牛奶没有问题，只不过会放臭P |
|
j******0
发帖数: 307 | 12 rt，求一个AmorePacific Treatment Enzyme Peel, 0.2 oz，$4/each,label ready
请站内联系！ |
|
|
d******y
发帖数: 57 | 14 国内朋友拜托我买Eternal Beauty by Enzyme.有哪位知道这是什么, 吃的还是用的?
在哪里能买到? 能往国内邮寄吗? |
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w******e
发帖数: 1187 | 15 一直用HRP－ABTS做ELISA，antibody signal强用的挺好。最近做aptamer
based ELISA，经常要develop1、2个小时，很是郁闷。请问有没有signal
amplification比较强劲的enzyme-substrate pair，产物是fluorescent
也行。//bow |
|
c**A
发帖数: 1474 | 16 才发现Ambion不卖这个东东了。
有人知道哪里可以搞到 guanylyltransferase enzyme 么？
我需要5'cap labelling my RNA transcripts.
多谢。。 |
|
N***e
发帖数: 6 | 17 Rodriguez-Paez, Lorena; Chena-Taboada, Miguel Angel; Cabrera-Hernandez,
Arturo; Cordero-Martinez, Joaquin; Wong, Carlos
Journal of Enzyme Inhibition and Medicinal Chemistry (2011), 26(4), 579-586.
Language: English, Database: CAPLUS
请热心的朋友帮查一下，谢谢！ |
|
x**y
发帖数: 147 | 18 首先感谢您进来.
如果您是做
1)甲烷单加氧酶
或者
2) 含铁模拟酶催化
或者
3) heme或non-heme iron-oxo enzyme
研究方面的,能否留下您的足迹或者或联系方式,我想和您请教一下这方面的基础(非常
基础)问题.
先谢谢! |
|
i***0
发帖数: 160 | 19 I have tried the Invitrogen enzyme-free cell dissociation solution,
dissociation is slower than trypsin, and milder. Better use that instead of
trypsin, if your assay is related to membrane proteins or cell membrane. |
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h*********g
发帖数: 18 | 20 我用maxiprep提取plasmid (2Kb)，跑胶得到2条带，还有些模糊的带，cut后也有几条
带，其中一条带是2kb, all the other bands are larger than the 2kb band。THe
restriction enzyme should only cut one site in the plasmid.Can I trust the
result? After that I will do the gel extraction. Why there are several bands
? Thanks. |
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k****o
发帖数: 728 | 21 外行请教： 有没有什么酶或者方法能够让protein disulfide bonds（最好是intra-
chain disulfide bond）scramble/exchange。 大概搜了一下，protein disulfide
isomerase （PDI） 好像是能够纠正incorrect disulfide bond回到原来的能让
protein稳定的correct disulfide bond。而我是想让正常的disulfide去和nearby的
disulfide bonds exchange，然后研究protein和其他target的binding。 不知道PDI能
不能做到这个？要怎么控制条件去故意打乱disulfide bond的配对？ 或者其他更好的
enzyme？
多谢！ |
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b****a
发帖数: 352 | 22 今年想去一个做蛋白进化
enzyme engineering, biocatalyst 方面的会议
有工业界相关性最好。
请问大牛门有什么好的推荐 谢谢 |
|
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f*******e
发帖数: 628 | 24 啥叫 qualify? 干什么用？
定量 oligo 的话，qbit 之类的 fluorescence 很准确，应该也不受 enzyme 的影响。
不怕麻烦的话，fluorometer 之类仪器加 staining dye 也可。
HPLC, ... 也行吧。跑个胶也行。用什么方法取决于你有多大量的 sample。 |
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d********w
发帖数: 1110 | 25 是的，就想qualify。比如说看看oligonucleotide放在含有enzyme的buffer里面一段时
间，是否变坏了分解了。 |
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s***e
发帖数: 911 | 26 如何保持type II restriction enzyme对DNA的specific binding的能力, 同时要
以致其cutting DNA的能力.
mg^{2+}好象对specific binding和cutting都很重要, 看来单纯控制mg^{2+}
含量不成.
谢谢. |
|
z*h
发帖数: 773 | 27 Cutting it from discussion.
This cellulose-to-amylose biotransformation could be scaled up by increasing
the stability of CBP and PGP and decreasing their production costs in terms
of $/kg enzyme (Fig. S6) because this combined cellulose-hydrolyzing and
starch-synthesizing enzyme cocktail did not involve any labile coenzymes [e.
g., CoA and NAD(P)] (Fig. 1A); no glucose released from the cellulose was
wasted (Fig. 1); only two more enzymes were added and they can be re-used
easily by a magnetic ... 阅读全帖 |
|
f**d
发帖数: 768 | 28 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
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发帖数: 1 | 29 You leave me no choice but . I can not stand someone without any chemistry
sense when he/she is a biologist.
Chemical reaction is a on/off equilibrium. Some enzymes pick up the first
substance first and then the second one if the space arrangement is allowed
in the active site; some enzymes bind to a coordinated substance complex if
the reaction needs both substances available at the same time to fit into
the active site. It depends on the nature of individual enzymes. Since ssDNA
can not bind t... 阅读全帖 |
|
S*******n
发帖数: 1721 | 30 【 以下文字转载自 AutismCare 俱乐部 】
发信人: Seattlian (Seattleite), 信区: AutismCare
标 题: 第六届自闭症和阿斯伯格大会——营养，锻炼
发信站: BBS 未名空间站 (Sat Nov 5 13:03:31 2011, 美东)
和营养相关的集体讨论笔记，里面提到一些关于改善挑食，维生素等的
话题我比较感兴趣。
Panelists:
Devin Houston, PhD - Biochemist, Scientific Consultant, former College
Professor, founder of Houston Enzymes
Jerry Kartzinel, MD - Physician, International Speaker, NY Times Best
Selling Author
Kimberly Mathai, MS, RD, CDE - Author, Registered Dietitian, Certified
Diabetes Educator
Benjamin Wahl, MSW -... 阅读全帖 |
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l*****3
发帖数: 12 | 31 不就是artificial enzyme complex么，09年nbt keasling那片，后来igem的nas，然后
vt zhang的angew都是这个套路么，这不就用个polymer代替下docker分子么？
Nat Nanotechnol. 2013 Feb 17. doi: 10.1038/nnano.2012.264. [Epub ahead of
print]
Biomimetic enzyme nanocomplexes and their use as antidotes and preventive
measures for alcohol intoxication.
Liu Y, Du J, Yan M, Lau MY, Hu J, Han H, Yang OO, Liang S, Wei W, Wang H, Li
J, Zhu X, Shi L, Chen W, Ji C, Lu Y.
Source
1] Key Laboratory of Functional Polymer Materials, Ministry of Education,
and I... 阅读全帖 |
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n**********g
发帖数: 196 | 32 OK, you want basic chemistry and here is basic chemistry.
“Since ssDNA
can not bind to the editing enzyme alone in vitro, the conclusion will be
the enzyme needs the second substance that is a conformation-ready genomic
DNA to form a functional enzyme-ssDNA-genomic target DNA complex to complete
the reaction. ”
----This is so wrong. There could be 100 reasons why an enzyme does not bind
a substance. For example, temperature, pH. Most importantly, it directly
contradicts Han’s data (Fig 1,2).
“As... 阅读全帖 |
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c********n
发帖数: 225 | 33 谈谈和蛋白打交道的日子 （一）
序
笔者已经做了多年和蛋白相关的科研与服务，产品研发生产以及产业化。在这里分享一
下与蛋白打交道这些年带来的一些感想。其实所有的科学研究都是用来解决人类已经和
即将遇到的各种各样的问题的，只有解决了问题才会有更大的存在价值。
第一篇 DNA测序， Sanger Style - 纪念Frederick Sanger
2013年11月20号，Frederick Sanger 去世了， 享年95岁。Sanger是DNA测序之父，获
得过两次诺贝尔奖， 一个是1958年蛋白测序， 另一个是1980年DNA测序。虽然DNA结构
发现的比蛋白晚， 但毕竟是genetic code，master script。ATCG四个碱基的组合比蛋
白的20个氨基酸的天文数字组合可更容易研究找规律了。人天生就是个好奇的动物，
最喜欢找规律, 尤其喜欢从简单的地方入手，所以围绕DNA的研究在Watson-Crick的碱
基配对这个简单地规律被发现以后，起来的就比蛋白快了很多。其实DNA和蛋白， 就像
是帅才和将才， 他们的用处是不同的，需要出色的帅才（DNA）订好了方向，能干的将... 阅读全帖 |
|
c********n
发帖数: 225 | 34 谈谈和蛋白打交道的日子 （一）
序
笔者已经做了多年和蛋白相关的科研与服务，产品研发生产以及产业化。在这里分享一
下与蛋白打交道这些年带来的一些感想。其实所有的科学研究都是用来解决人类已经和
即将遇到的各种各样的问题的，只有解决了问题才会有更大的存在价值。
第一篇 DNA测序， Sanger Style - 纪念Frederick Sanger
2013年11月20号，Frederick Sanger 去世了， 享年95岁。Sanger是DNA测序之父，获
得过两次诺贝尔奖， 一个是1958年蛋白测序， 另一个是1980年DNA测序。虽然DNA结构
发现的比蛋白晚， 但毕竟是genetic code，master script。ATCG四个碱基的组合比蛋
白的20个氨基酸的天文数字组合可更容易研究找规律了。人天生就是个好奇的动物，
最喜欢找规律, 尤其喜欢从简单的地方入手，所以围绕DNA的研究在Watson-Crick的碱
基配对这个简单地规律被发现以后，起来的就比蛋白快了很多。其实DNA和蛋白， 就像
是帅才和将才， 他们的用处是不同的，需要出色的帅才（DNA）订好了方向，能干的将... 阅读全帖 |
|
K*F
发帖数: 120 | 35 请您转发 z******[email protected]
万分感谢！祝您身体健康，财源广进！
Biochim Biophys Acta. 1988 Apr 28;954(1):1-13.
The inducible trimethylamine-N-oxide reductase of Escherichia coli K12:
biochemical and immunological studies.
Silvestro A, Pommier J, Giordano G.
Source
Laboratoire de Structure et Fonction des Biomembranes, CNRS, Marseille,
France.
Abstract
The inducible trimethylamine-N-oxide reductase which migrates on non-
denaturing polyacrylamide gels with an RF of 0.22, has been purified from
the soluble fraction o... 阅读全帖 |
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n**********g
发帖数: 196 | 36 1) The current low (zero) verified success rate is sufficient for people to
question the story.
When will your statistic data collection be completed? Let's talk about the
success
rate then.
Your comments are so interesting. If you do not know the science, just be
patient and wait for more answers.
2) “Do you think 1.3 mm difference (resolution)
can be achieved on peaks with a width of 1.5-2 mm?” The answer is yes
because of Fig 4e. Your statement is “difference
sample preparation” is completely... 阅读全帖 |
|
发帖数: 1 | 37 Before I start, I’d like to make my point crystal-clear. This will be
serving as my
last post on this subject and I am not interested in any college-level
discussion.
How soon do you want to close your statistical data collection? I guess
this time window of reproducibility will be determined by the journal and
the whole scientific community. Dr. Han’s fate does not concern me from
the beginning. However, I do care the quality of arguments against Dr. Han’
s
paper written up by some green-hand... 阅读全帖 |
|
A*******s
发帖数: 9638 | 38 "Empty Nose" Syndrome
What Is Empty Nose Syndrome?
What are Turbinates?
Turbinate Surgeries
How do you prevent ENS?
Treatment
What Is Empty Nose Syndrome?
The nasal turbinates are shelf-like structures in the nasal cavity (which
begins where the inside of your nose enters your head). They serve to
provide moisture, warmth, and airflow for breathing, and many of the body's
natural defenses against infection. There are a variety of different
problems associated with the turbinates. Sometimes the t... 阅读全帖 |
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y********o
发帖数: 2565 | 39 A similar argument here:
http://askville.amazon.com/people-lactose-intolerant/AnswerView
Who Gets Lactose Intolerance
Normally when a person eats something containing lactose, an enzyme in the
small intestine called lactase breaks down lactose into simpler sugar forms
called glucose and galactose. These simple sugars are then easily absorbed
into the bloodstream and turned into energy — fuel for our bodies.
People with lactose intolerance do not produce enough of the lactase enzyme
to break ... 阅读全帖 |
|
w*********g
发帖数: 30882 | 40 基因武器比核弹更可怕
综合《看世界》、《扬州日报）消息，2003年发生的非典疫情，全球确诊8437个病
例，死亡813人。非典病毒若复制成基因武器，将是人类的灾难。这种忧虑绝非祀人忧
天。2006-2007年，以色列科学家奥登里奇将携有名为“以色列急性麻痹病毒”的实验
用蜜蜂带到美国，少数蜜蜂意外逃跑，导致美国35个州的家养蜜蜂一起远走高飞，有些
养蜂场一小时内“出走”的蜜蜂就占到70％以上，造成北美农林产业140亿美元损失。
这种病毒正是奥登里奇研制的基因武器之一！
基因武器有3种类型
基因武器又称遗传工程武器或DNA武器，是采用遗传工程技术，用工程设计的办法
，通过基因重组，在一些致病细菌或病毒中接入能对抗普通疫苗或药物的基因，或者在
一些本来不会致病的微生物体内接人致病基因而制造成生物武器。它能改变非致病微生
物的遗传物质，使其产生具有显著抗药性的致病菌，利用人种生化特征上的差异，使这
种致病菌只对具有特定遗传特征的人们产生致病作用，从而有选择地消灭敌方有生力量。
目前，世界基因武器的发展... 阅读全帖 |
|
C*I
发帖数: 4736 | 41 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 42 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
|
C*I
发帖数: 4736 | 43 Published: 30 October 2013
Isolation and characterization of a bat SARS-like coronavirus that uses the
ACE2 receptor
Xing-Yi Ge, Jia-Lu Li, Xing-Lou Yang, Aleksei A. Chmura, Guangjian Zhu,
Jonathan H. Epstein, Jonna K. Mazet, Ben Hu, Wei Zhang, Cheng Peng, Yu-Ji
Zhang, Chu-Ming Luo, Bing Tan, Ning Wang, Yan Zhu, Gary Crameri, Shu-Yi
Zhang, Lin-Fa Wang, Peter Daszak & Zheng-Li Shi
Nature volume 503, pages535–538(2013)Cite this article
Abstract
The 2002–3 pandemic caused by severe acute respirator... 阅读全帖 |
|
J*V
发帖数: 3150 | 44 基因武器已成为现实。
直言了，2012-03-13 | 2012-03-14 22:05:20。
http://zhiyanle.blog.hexun.com/73735052_d.html
两会上，有代表阐明了转基因技术的危害风险、说明了它可用来做杀伤武器，由此而提
出了中国需要建立“生物国防”体系的建议，其中包括对转基因技术产品的严格检验、
监控乃至限制或禁止等措施的建议。该建议妨碍了转基因既得利益集团的商业利益，于
是，他们雇用的枪手文人接连出来，说转基因技术可制造杀伤武器的说法是“危言耸听
”云云。其实，在美国，用转基因技术搞基因武器不但已经是现实了，而且已经开始公
开征集相关国防用品设计了。故事很多，下面就说最近发生的一个故事。
2012年开始，美国国防系统的“达尔帕”（DARPA）部门公开招标“蛋白折叠”软件设
计，项目名称是“FOLD-IT”（直译：折叠它）。所谓“蛋白折叠”，就是根据某基因
结构而形成某蛋白的过程；该蛋白发挥功能可使某生命体幸存发育或残疾死亡。要实现
某种预期的蛋白折叠，就先要实现某种基因结构。到目前，基因结构设计包括DNA-水平
和RNA-水平的两种设... 阅读全帖 |
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wh
发帖数: 141625 | 45 Cancer Update from John Hopkins
約翰霍普金斯最新之癌症研究報導
1. Every person has cancer cells in the body. These cancer cells do not show
up in the standard tests until they have multiplied to a few billion. When
doctors tell cancer patients that there are no more cancer cells in their
bodies after treatment, it just means the tests are unable to detect the
cancer cells because they have not reached the detectable size.
每一個人身上都帶有癌細胞，除非癌細胞擴增至幾十億個數量時癌細胞是無法在一般檢
查時被檢查出來的。所以每當病患治療到一個階段後醫生會告訴病患其體內已無癌細胞
時，醫生所指的是其體內... 阅读全帖 |
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wh
发帖数: 141625 | 46 【 以下文字转载自 Connecticut 讨论区 】
发信人: wh (wh), 信区: Connecticut
标 题: 約翰霍普金斯最新之癌症研究報導（ZZ）
发信站: BBS 未名空间站 (Sat Oct 30 13:44:19 2010, 美东)
Cancer Update from John Hopkins
約翰霍普金斯最新之癌症研究報導
1. Every person has cancer cells in the body. These cancer cells do not show
up in the standard tests until they have multiplied to a few billion. When
doctors tell cancer patients that there are no more cancer cells in their
bodies after treatment, it just means the tests are unable to detect the
cancer cells because th... 阅读全帖 |
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l*****a
发帖数: 38403 | 47 http://www.veganhealth.org/b12/plant
There has been a long history of misconceptions about which, if any, plant
foods are sources of B12. Much of this
stems from the methods of measuring B12 analogues. Other confusion stems
from bacterial contamination that occurs
in some foods but not others. Please see Measuring B12 in Plant Foods: Why
the Confusion? for an explanation of
the methods for for measuring B12 analogues in plant foods.
Unlike animals, most, if not all, plants have no B12 requiremen... 阅读全帖 |
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a**u
发帖数: 8107 | 48 ☆─────────────────────────────────────☆
xiaoputi (xiaoputi) 于 (Mon Nov 1 02:08:36 2010, 美东) 提到:
我也是这种状态，就像人家说：吃素容易，成为一个坚定的素食者难。
还记得两年前，朋友介绍MITBBS给我打发时间，我一来就扎在WISDOM里不走了，而那个
时候正是该版最热闹的时候，慢慢地WAICHI走了，RUNSUN走了，版上一天天安静着。看
来以前这里的热闹都是来看把戏的，真正讨论佛法研习的没有几个。或许佛法应该自修
自己体悟，而不适合讨论？
都是清闲的人。
☆─────────────────────────────────────☆
omnbmh (HaHong) 于 (Mon Nov 1 02:30:15 2010, 美东) 提到:
不要听人说，要听自己说。他们说难，你就觉得难啊？自己要为自己作主的
☆─────────────────────────────────────☆
thelostsheep (迷失的羊) 于 (Mon Nov 1 03:57:... 阅读全帖 |
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P******e
发帖数: 129 | 49 Responsibilities:
The successful candidate will join a dynamic team and be responsible for
analyzing a variety of enzyme containing complex mixtures and characterizing
the enzyme and certain non-enzymatic components.
• Conduct enzymatic screening assays, and reliable determinations
of enzymatic activities in a variety of samples to support new enzyme
discovery;
• Develop and optimize various biochemical assays;
• Demonstrate the ability to correctly analyze and interpr... 阅读全帖 |
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P******e
发帖数: 129 | 50 If you are interested, please contact me. Thanks,
Responsibilities:
The successful candidate will join a dynamic team and be responsible for
analyzing a variety of enzyme containing complex mixtures and characterizing
the enzyme and certain non-enzymatic components.
• Conduct enzymatic screening assays, and reliable determinations
of enzymatic activities in a variety of samples to support new enzyme
discovery;
• Develop and optimize various biochemical assays;
• Demons... 阅读全帖 |
|
