M****e
发帖数: 70 | 1 my point is to make sure that you have transfer of good
quality and quantity of RNA onto the blotting material.
the washing stringency for your experiment is not quite
high (i.e., 42C). i think the problem is mainly due to
bad RNA or probe. if there is a lot of radioactive
nucleotides incorporated into the probe, you should
detect very hot signal after purification. denature the
probe before use. GAPDH is a housekeeping gene that is
typically used for normalization of load. i suggest you
read a |
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