m******5
发帖数: 1383 | 1 I found some claimed to be 'Chip qualified' antibody naturally gave out
super strong background (when using non transfected or knock out cell line
as negative control), maybe due to non specific binding to DNA or Histon?
I start to wonder if those paper simply use IgG as negative control
really got validated result. |
d****7
发帖数: 109 | 2 嗯，确实，比如Abcam的GFP antibody (ab290)，跑western看一眼，好多好多非特异带
，结果N多人用这个做ChIP-seq
【在 m******5 的大作中提到】
: I found some claimed to be 'Chip qualified' antibody naturally gave out
: super strong background (when using non transfected or knock out cell line
: as negative control), maybe due to non specific binding to DNA or Histon?
: I start to wonder if those paper simply use IgG as negative control
: really got validated result.
|
m******5
发帖数: 1383 | 3 any reference? I am so digging this!!
【在 d****7 的大作中提到】
: 嗯，确实，比如Abcam的GFP antibody (ab290)，跑western看一眼，好多好多非特异带
: ，结果N多人用这个做ChIP-seq
|
d****7
发帖数: 109 | 4 check these two cell papers:
Quantitative Interaction Proteomics and Genome-wide Profiling of Epigenetic
Histone Marks and Their Readers
SHREC, an effector complex for heterochromatic transcriptional silencing
【在 m******5 的大作中提到】
: any reference? I am so digging this!!
|
i*****g
发帖数: 11893 | 5 就是这样的，有些CHIP的paper，就是这么做的，
只要有对照，
只要有统计
只要编故事
就有了paper
其实都是垃圾 |
m******5
发帖数: 1383 | 6 我就是买到个垃圾抗体出来抱怨一下。。怎么chip还成房地产泡沫了。。。
【在 i*****g 的大作中提到】
: 就是这样的，有些CHIP的paper，就是这么做的，
: 只要有对照，
: 只要有统计
: 只要编故事
: 就有了paper
: 其实都是垃圾
|
