z*******a
发帖数: 175 | 1 one antibody needs to be incubated in 5% milk for 2 hours at room
temperature for best results.
already one hour and want to go home now. wondering whether it is OK if I put it at 4 degree for overnight. |
s******y
发帖数: 28562 | 2 Overnight
put it at 4 degree for overnight.
【在 z*******a 的大作中提到】
: one antibody needs to be incubated in 5% milk for 2 hours at room
: temperature for best results.
: already one hour and want to go home now. wondering whether it is OK if I put it at 4 degree for overnight.
|
s******y
发帖数: 28562 | 3 Yes it will be alright
put it at 4 degree for overnight.
【在 z*******a 的大作中提到】
: one antibody needs to be incubated in 5% milk for 2 hours at room
: temperature for best results.
: already one hour and want to go home now. wondering whether it is OK if I put it at 4 degree for overnight.
|
z*******a
发帖数: 175 | 4 thanks
you are always helpful
2 h @ RT =O/N @4 C? |
b******k
发帖数: 1874 | 5 各个抗体都不一样的。特异性好，你可以室温，否则一般都要4度过夜。时间和滴度也有
关系。
【在 z*******a 的大作中提到】
: thanks
: you are always helpful
: 2 h @ RT =O/N @4 C?
|
n******d
发帖数: 1055 | |
