n********k
发帖数: 2818 | 1 Hi, folks:
Any of you do primary mouse neuron culture? I like to know which stage
one can easily derive primary
neurons, the later the better for me... We have protocols for embryonic
neurons but I'll like something later
than that but aslo need to be healthy and can tolerate with some
manipulation later on such as Cre-viral
infection.... |
p*******r
发帖数: 4048 | 2 P1
【在 n********k 的大作中提到】
: Hi, folks:
: Any of you do primary mouse neuron culture? I like to know which stage
: one can easily derive primary
: neurons, the later the better for me... We have protocols for embryonic
: neurons but I'll like something later
: than that but aslo need to be healthy and can tolerate with some
: manipulation later on such as Cre-viral
: infection....
|
n********k
发帖数: 2818 | 3 could you please elaborate a bit? thanks, buddy
【在 p*******r 的大作中提到】
: P1
|
G******O
发帖数: 189 | 4 I use p1-3 usually. Occasionally old pups such as p20 can also be used but
you might get only several thousands cells per ml and those cells might not
grow very well. So the younger the better.
【在 n********k 的大作中提到】
: Hi, folks:
: Any of you do primary mouse neuron culture? I like to know which stage
: one can easily derive primary
: neurons, the later the better for me... We have protocols for embryonic
: neurons but I'll like something later
: than that but aslo need to be healthy and can tolerate with some
: manipulation later on such as Cre-viral
: infection....
|
S*****s
发帖数: 287 | 5 时间越长 neuron 越不容易培养，到最后长出来的都是 glial cell。还是尽量取年幼
的老鼠做效果比较好。 |
