h*******o
发帖数: 4884 | 1 For primary neurons, we use neurobasal media +B 27. It is selective towards
neurons and glia usually cannot survive for long term in NBM+B27. With that
said, there is no pure neuronal culture. Anyway, cultured neurons do exhibit
some glial properties, such as glucose transporter expression, metabolic
activity, etc.
For embryonic glial culture, we use DMEM/F12 (1:1) +10% FBS. Because glial
cells are mitotic whereas neurons are post-mitotic, neurons will be easily
outnumbered by proliferative glia... 阅读全帖 |
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t********9
发帖数: 536 | 2 我现在做一些神经生物学方面的实验，我 Apply LB buffer to a xx neuron for 10s,
然后switch to a stimulus and apply it to this neuron for 60s, 然后再switch
to LB buffer and apply It to this neuron for 60s, 发现这个Neuron 的calcium
response 一直呈下降的趋势, 并没有按设想的那样在apply LB 后回到baseline. 请教
两个问题：
1. 为什么这个neuron在applied stimulus 后一直呈下降趋势，即使在applied LB
buffer 之后？
2. 我能得出结论： this neuron is inactivated by this stimulus?
谢谢！ |
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w*********s
发帖数: 618 | 3 神经科学顶级期刊Neuron刊登南京大学王建军教授、朱景宁副教授课题组研究成果
[发布时间：2011-03-07] [字体大小： 小 中 大]
神经科学顶级期刊Neuron（神经元，系Cell Press杂志社出版的Cell杂志姐妹
期刊）最近刊登了南大生命科学学院和生物医药技术国家重点实验室王建军教授、朱景
宁副教授课题组的研究论文“A role for orexin in central vestibular motor
control”（Neuron, 2011, 69:793-804）。
Orexin是一种新近发现的小分子神经肽，因其强烈的促食欲作用而得名。已经
发现，起源于下丘脑外侧区/穹窿周区的中枢orexin能神经系统在机体能量代谢、药物
成瘾、奖赏和情绪反应等许多非躯体性活动中发挥重要功能。有趣的是，orexin缺乏可
致人和动物发生嗜睡-猝倒症（narcolepsy- cataplexy），一种表现为白天无法用意志
控制的过多睡眠发作以及毫无预警的突然昏睡或猝倒。该病遍发于世界各地，且不分年
龄种族。猝倒发作时患者虽意识清醒，但却因躯体... 阅读全帖 |
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b****h
发帖数: 18 | 4 the idea is to knock in Neo in hESC, driven by neuron-specific promoter,
then differentiated into neuron. Only specific neuron can survive under G418
pressure. I also think it will be slow, puromycin is faster? I am not sure
for neuron, as they cannot proliferate.Any experience will be highly
appreciated. Facs cannot be used for attached neurons. |
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f**d
发帖数: 768 | 5 一些人认为，人们总是可以从失败中学到东西，但大脑的工作原理似乎并非如此。
美国一项最新研究认为，失败的经历几乎对大脑不起作用，脑细胞从成功的经验中
学到更多东西。
美国麻省理工学院的研究人员根据实验中动物脑细胞的不同反应得出以上结论。研
究人员创设了一种独特的“快照”系统，以反映脑细胞的学习过程，在提供正确和
错误行为的信息后，实时拍下单个脑细胞的反应。
研究人员在一份声明中说：“脑细胞可以记录近期的行为是否成功。当某一行为成
~~~~~~~~~~~
功时，脑细胞会针对所学内容进行更加微细的调整。而当失败发生时，脑部几乎没
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
有发生任何变化，行为也没有获得改善。”
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
这项研究成果刊登在美国《神经元》月刊上。（生物谷Bioon.com）
Neuron, Vol... 阅读全帖 |
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b****h
发帖数: 18 | 6 本来不怀疑这一点，但有同事说G418适用于杀死能增值的细胞。我最近打算赋予特定类
型的 neuron具有neo或puro的抗性，然后杀死其他类型的neurons和杂细胞。有哪位做
神经的兄弟用过G418或PUROMECIN筛选/杀死neuron的经验吗？我指的是元代老鼠或人的
neuron。多谢！！！ |
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n********k
发帖数: 2818 | 7 Hi, folks:
Any of you do primary mouse neuron culture? I like to know which stage
one can easily derive primary
neurons, the later the better for me... We have protocols for embryonic
neurons but I'll like something later
than that but aslo need to be healthy and can tolerate with some
manipulation later on such as Cre-viral
infection.... |
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k*****1
发帖数: 454 | 8 本人非牛人，只是说说个人的看法：
“bursting 和 non-bursting”虽然不能说是神经元的普遍现象，但是在相当一部分的
neuron里面也存在，所以如果你投到的是比较好的杂志上投，编辑可能会认为你建造的
是一个比较“generalized”的神经元，而不是那个特定的调控嗅觉神经元的neuron。
我觉得是不是你只抓住该neuron的一个特点来建模，所以让编辑觉得太简单了点？ |
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s*****e
发帖数: 172 | 9 本人化学系的学生，要用到含有axon的 neuron细胞， 听人说过ATCC可以买，但是不知
道哪种neuron才含有axon
谢谢先！ |
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a****d
发帖数: 1919 | 10 最新一期的Nature paper，reprogramming from fibroblasts to functional neurons
. 一样的套路，不过还是感觉很crazy！
Direct conversion of fibroblasts to functional neurons by defined factors
Thomas Vierbuchen1,2, Austin Ostermeier1,2, Zhiping P. Pang3, Yuko Kokubu1,
Thomas C. Südhof3,4 & Marius Wernig1,2
Abstract
Cellular differentiation and lineage commitment are considered to be robust
and irreversible processes during development. Recent work has shown that
mouse and human fibroblasts can be reprogrammed to a plurip |
|
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f**u
发帖数: 346 | 12 我有两个个不内行的问题，除了哈佛那片从胰腺细胞变胰岛细胞的，
其他的成功例子是不是都是用fibroblast做的？有用其他类型细胞的吗？
另外，我觉得直接从fibroblast到neuron还不如中间经过iPS的阶段，
因为那样在iPS的时候可以扩增细胞数量。
不知道用他们这三个同样的因子有没有可能让iPS变neuron。
is |
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d******r
发帖数: 124 | 13 有什么诀窍？既减少neuron death又增加转染效率？
我按照Gong Chen的Nature Protocol来做，发现转染之后99%的neuron都挂了... |
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t*d
发帖数: 1290 | 14 最近接到一个项目，需要找 neuron secreted proteins。有没有这方面的 proteomics
的报导？找到一堆 astrocyte 的，就找不到 neuron 的。
谢谢了先！ |
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a****d
发帖数: 1919 | 15 Tunel is not neuron specific, not sure about Fluro Jade.
To look at neurons, you might be able to double stain with NeuN or other
markers. |
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n*******n
发帖数: 515 | 16 版上高手众多，如果知道答案，不吝赐教。
我是做有机合成的，平时也会养一些细胞, 主要是cancer cell lines。
这次被老板指派了一个project需要primary neuron cells。这可从来没做过，试了几
次，可每次harvest neuron cells用trypsin或是scraping的话要不然下不来，要不然
百分之六七十的细胞已经挂了。。。
请有经验的同学指点一下吧，有包子。谢谢 |
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m*********n
发帖数: 215 | 17 你是把primary neuron种在什么表面上？ falcon dish? coated with what?
还有你收集neuron后准备用来做什么？ |
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h*******o
发帖数: 4884 | 18 It is a lot harder to culture adult neurons than embryonic neurons.
Our lab has tried for quite a while with no success.
If you are interested, take a look at Greg Brewer's Nature Protocol paper.
Good Luck |
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a****d
发帖数: 1919 | 19 The radial glia cells divide asymmetrically and produce precursor/immature
neurons, which migrate along the radial process up towards the cortical
plate.
Neurons were born first,the glia cells were produced later during cortical
development. Both are originated from the radial glia cells. Recently there
are findings suggesting that glia cells divide locally at the upper cortex
to increase the glia population.
Hopefully this is helpful. |
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L*********y
发帖数: 322 | 20 If you haven't published your paper yet, it is useless asking this question.
Science and Neuron/NN are all top journals in neuroscience field, and many
research papers published in Neuron/NN are even better than those in Science
. The insiders will focus more on the quality of your research work instead
of which journal it goes to. |
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L*********y
发帖数: 322 | 21 If you haven't published your paper yet, it is useless asking this question.
Science and Neuron/NN are all top journals in neuroscience field, and many
research papers published in Neuron/NN are even better than those in Science
. The insiders will focus more on the quality of your research work instead
of which journal it goes to. |
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t***s
发帖数: 163 | 22 Science 13 March 2009:
Vol. 323. no. 5920, p. 1429
DOI: 10.1126/science.1155416
Prev | Table of Contents | Next
Technical Comments
Comment on "Detection, Stimulation, and Inhibition of Neuronal Signals with
High-Density Nanowire Transistor Arrays"
Peter Fromherz* and Moritz Voelker
Patolsky et al. (Reports, 25 August 2006, p. 1100) used silicon nanowires to
record action potentials in rat neuronal axons and found increases in
conductance of about 85 nanosiemens. We point out that the data co |
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t******0
发帖数: 629 | 23 小弟是设计硬件存储器的。
曾经用一个矩阵式的存储来实现neuron network，即对于任意N个神经元的网络，我都
可以用一个 N x N 的crossbar 来实现：
N条row线（dendrite）;
N条column线(axon);
第i行第j列的交点处的数据代表第i号神经元和第j号的神经元之间的synapse的weight。
这样无论什么样的网络连接都可以实现。但是问题是浪费硬件资源(N x N)！
一般的神经网络，neuron是分为好几层的，每层内部是没有互联的，只有层之间是有互
联的，对吧。
如果我仍然用以上行和列的方法来存储synapse的weight信息，如何才能不浪费硬件资
源（比如利用整齐而远少于N x N的设计）？有类似的资料可以推荐一下吗？
谢谢啊。 |
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z***b
发帖数: 4667 | 24 那个cover letter里面的title怎么写
我之前给其他journal投稿的时候都是
XXX
Editor-in-chief, YYY journal
Dear Dr. XXX
...
准备投到Neuron上，结果查不到谁是editor-in-chief，该怎么办啊？
谢谢 |
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L*********y
发帖数: 322 | 25 Katja Brose.
"Dear Editor" should be fine as Neuron has many manuscript-handing editors. |
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c****n
发帖数: 587 | 26 国内的亲戚得了运动神经元病(Motor Neuron Disease MND)，在国内去的神经科最好的
北医三院，后来专家会诊都说没
法治疗。
请问这种病在美国有什么办法吗？谢谢 |
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G*****3
发帖数: 92 | 27 妈妈半年前发现左腿无力，走路频繁摔跤，无法上楼，走路需要用walking frame. 最
近开始右腿也
觉得无力，晚上肌肉跳动。检查了MRI（脑，颈椎和下腰）， LUMBAR PUNCH，
ELECTRICY CONDUCTION。 只有elcetricity conduction 结果不好，其他结果都正常。
血液检查蛋白质含量，钾，钠，维生素B12 也都正常。医生初步怀疑是Motor Neuron
Disease.
我想请教一下版上的专家们，这种病要如何确诊？还有如果得了这种病，是不是最后都
会从下肢，发展到上肢，进而影响语言，吞咽和呼吸功能？ |
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w*********r
发帖数: 142 | 28 Hi,I would ask if someone knows about Motor neuron disease--Amyotrophic
lateral sclerosis (ALS) here.
The patient is taking Riluzole and but her conditions is becoming worse.
I would ask for help if anyone can provide any information if there is new
medicine available now in USA. |
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h****f
发帖数: 175 | 29 帮老板审过CNS，自己独立审稿还没攒够。neuron方面都行，不胜感激。 |
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a***m
发帖数: 5037 | 30 维基百科上这一段简明 清楚
In machine learning and cognitive science, artificial neural networks (ANNs)
are a family of models inspired by biological neural networks (the central
nervous systems of animals, in particular the brain) and
are used to estimate or approximate functions
================================================记住这句就好
that can depend on a large number of inputs and are generally unknown.
Artificial neural networks are generally presented as systems of
interconnected "neurons" which exchange ... 阅读全帖 |
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a*****a
发帖数: 312 | 31 Did someone (puppeteer?) mention Zhuang Xiaowie get some NIH big grant
on imaging the neuron networks in whole brain?
some info I can remember: it is a collaboration between Zhuang and some
others. Zhuang provides the super-resolution imaging techniques. Others
can make thin slice of brain and reconstruct the images together etc...
Could someone provide a link for more info on that?
Thanks. |
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a****d
发帖数: 1919 | 32 I thought the key factor here is the Chromatin remodeling protein. Crabtree
from Stanford has done really nice study to illustrate that different BAF
subunits specifically expressed in different stages of neural lineage.To
switch fibroblast to functional neuron, a large group of genes have to be re
-regulated, probably at the chromatin assembly/disassembly level. It would
be interesting to see if more related study can come up, basically combining
specific chromatin remodeling factor with cell f |
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n********k
发帖数: 2818 | 33 think it a bit differently...what would be the easiest cells to get and also
happen to be cycling but differentiated cells...there are certainly other
cell types...however either hard to get or terminated differentiated and non
-cycling, I don't suppose anyone wants to RP cardiocyte, myofibers, neuron
or B cells for routine therapeutic purpose, agree? Shinya was saying even a
single hair may be too much for him (as he is bald) to donate for iPS...
there are plenty of examples of reprogramming o |
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n********k
发帖数: 2818 | 34 absolutely Yes, I think we shall see reports on them within a year or so...
and in theory it could be even easier than neuron... |
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d******r
发帖数: 124 | 35 有什么transfection reagent推荐？我试了磷酸钙，neuroFECT,GenCarrier-2，效果都
不理想，转染效率很低（平均2，3个/8000 neuron），死掉很多。因为需要等轴突树突
分化之后再转染，所以不能用电转和amaxa。用病毒又太费时。各位大侠有什么经验可
以传授吗？ |
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a****d
发帖数: 1919 | 36 for Ca2+ P method, make sure the pH~ 7.04; after adding the mixed plamids,
buffer, wait only half hour, then wash out the transfection medium as clean
as possible(you should be able to see only the black aggregates on the cell
surface, but not other places), then add fresh warm culture medium.
You can also try Fugene HD from Roche, which is better and less toxic
compared to lipofectamin and similar products.
you can also grow cortical neurons on top of glia cells to preserve activity
and help ce |
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S*****s
发帖数: 287 | 37 时间越长 neuron 越不容易培养，到最后长出来的都是 glial cell。还是尽量取年幼
的老鼠做效果比较好。 |
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n********k
发帖数: 2818 | 38 Could any friends recommend nice and simply ways to look at mature neuron
dendritic morphology in hippocampus (CA regions and DG as well). my Cre is
so bright and GFP is so weak, hard to tell...not sure whether my sections
still can go for Golgi staining since we perfused with PFA and then sucrose
and then section at 30um...I am thinking to go with calbindin... |
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o*****n
发帖数: 445 | 39 现在在e coli里表达一个蛋白，这个蛋白序列里有cystein。我想知道蛋白纯化时需不
需要加入dtt.
蛋白是neuron dendrites里的一个蛋白,不知道dendrite里是不是reducing
environment?
谢谢。 |
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M*****n
发帖数: 16729 | 40 block protein synthesis, neuron当然也能杀掉，就是慢点 |
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m*********n
发帖数: 215 | 41 这个要很慢的吧。。。不能用其他marker标记你要的neuron么？譬如带个荧光然后facs
？ |
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j*********o
发帖数: 237 | 42 想做一个实验，需要把小鼠ES细胞诱导成neural progenitor和neuron，只知道一般使
用RA诱导，但找不到具体的protocol，版上有没有哪位做过有比较完整的protocol？能
不能分享一下，非常感谢！如有请发到d********[email protected]。多谢多谢！！ |
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z****g
发帖数: 3340 | 43 Pubmed Pierre Chambon with key words RA neuron. |
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b*********8
发帖数: 97 | 44 适用于neuronal culture 和 brain section. 方法太多了, 不知道哪些比较快速实用
点?
谢谢! |
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f******r
发帖数: 96 | 45 for cultured neuronal: MTT,CTB,LDH;or PI staining;
for brain section: you'd bette to count the neuorn number~~ |
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b*********8
发帖数: 97 | 46 Is Tunel staining neuron specific? What about Fluoro Jade? |
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