b****h
发帖数: 18 | 1 本来不怀疑这一点，但有同事说G418适用于杀死能增值的细胞。我最近打算赋予特定类
型的 neuron具有neo或puro的抗性，然后杀死其他类型的neurons和杂细胞。有哪位做
神经的兄弟用过G418或PUROMECIN筛选/杀死neuron的经验吗？我指的是元代老鼠或人的
neuron。多谢！！！ |
b****r
发帖数: 17995 | |
M*****n
发帖数: 16729 | 3 block protein synthesis, neuron当然也能杀掉，就是慢点
【在 b****h 的大作中提到】
: 本来不怀疑这一点，但有同事说G418适用于杀死能增值的细胞。我最近打算赋予特定类
: 型的 neuron具有neo或puro的抗性，然后杀死其他类型的neurons和杂细胞。有哪位做
: 神经的兄弟用过G418或PUROMECIN筛选/杀死neuron的经验吗？我指的是元代老鼠或人的
: neuron。多谢！！！
|
m*********n
发帖数: 215 | 4 这个要很慢的吧。。。不能用其他marker标记你要的neuron么？譬如带个荧光然后facs
？ |
b****h
发帖数: 18 | 5 the idea is to knock in Neo in hESC, driven by neuron-specific promoter,
then differentiated into neuron. Only specific neuron can survive under G418
pressure. I also think it will be slow, puromycin is faster? I am not sure
for neuron, as they cannot proliferate.Any experience will be highly
appreciated. Facs cannot be used for attached neurons. |
M*****n
发帖数: 16729 | 6 using Neo(r) is not a good idea to do this negative selection.
read more papers and you will find better selection markers.
G418
sure
【在 b****h 的大作中提到】
: the idea is to knock in Neo in hESC, driven by neuron-specific promoter,
: then differentiated into neuron. Only specific neuron can survive under G418
: pressure. I also think it will be slow, puromycin is faster? I am not sure
: for neuron, as they cannot proliferate.Any experience will be highly
: appreciated. Facs cannot be used for attached neurons.
|
