l****y
发帖数: 398 | 1 any literature?
it would be great if the method is compatible with the wanner's. Thanks! |
X***n
发帖数: 366 | 2 What does it mean to make conditional knock out in a single cell prokaryote? |
l****y
发帖数: 398 | 3 to turn off the expression of certain gene at a specific time point.
prokaryote?
【在 X***n 的大作中提到】
: What does it mean to make conditional knock out in a single cell prokaryote?
|
X***n
发帖数: 366 | 4 how about using pKD46 to flank the gene you want to knock out with FRT,then
insert FLP gene downstream the promoter that activate at the specific time
point?
I don't know details :) |
l****y
发帖数: 398 | 5 has anyone actually done it in this way?
then
【在 X***n 的大作中提到】
: how about using pKD46 to flank the gene you want to knock out with FRT,then
: insert FLP gene downstream the promoter that activate at the specific time
: point?
: I don't know details :)
|
X***n
发帖数: 366 | 6 I am engineering the DH5alpha chromosome so that it can flp out fragment in
my plasmid.
【在 l****y 的大作中提到】
: has anyone actually done it in this way?
:
: then
|
l*****h
发帖数: 59 | 7 Use Targetron from Sigma. |
l****y
发帖数: 398 | 8 thanks. But how to do conditional KO with it? I can't find it in their
manual.
【在 l*****h 的大作中提到】
: Use Targetron from Sigma.
|
