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发帖数: 70 | 1 for genomice southern, you should have very good quality DNA
and digest it completely. it is better to purify the gDNA
and remove anything that may inhibit digestion. typically,
i would digest about 10-20ug of gDNA O/N with enough enzyme
and add another shot next day. it is not suggested to add
EtBr into the gel, though i found it was fine with mine.
you should follow a good protocol that is known to work for
genomic southern. my opinion is that transfer should be very
good. after depurination a |
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