L******e
发帖数: 679 | 1 The following experiments were finished:
1: gene expression is increased significantly in tumor compared with the
corresponding normal tissue (RT-PCR for mRNA)
2: Protein expression in tumor cell is significantly increased (western blot)
3: Cell migration assay showed the stimulation in the overexpressing cells.
4; Gene silencing indicated the inhibition of some 'stree', which is related
to the gene.
What else need to do? Is thre a routine rule for the approval of an oncogene?
Thanks. |
C*****h
发帖数: 926 | 2 1. in vitro transformation assays (eg. soft agar assay, focus formation
assay)
2. in vivo over-expression to examine if tumor formation can be induced
blot)
related
oncogene?
【在 L******e 的大作中提到】
: The following experiments were finished:
: 1: gene expression is increased significantly in tumor compared with the
: corresponding normal tissue (RT-PCR for mRNA)
: 2: Protein expression in tumor cell is significantly increased (western blot)
: 3: Cell migration assay showed the stimulation in the overexpressing cells.
: 4; Gene silencing indicated the inhibition of some 'stree', which is related
: to the gene.
: What else need to do? Is thre a routine rule for the approval of an oncogene?
: Thanks.
|
b**********8
发帖数: 349 | 3
very good ideas~
I Cannot agree with you any more.
【在 C*****h 的大作中提到】
: 1. in vitro transformation assays (eg. soft agar assay, focus formation
: assay)
: 2. in vivo over-expression to examine if tumor formation can be induced
:
: blot)
: related
: oncogene?
|
N******n
发帖数: 3003 | 4 name that gene: Oncogene1 |
f*******e
发帖数: 354 | 5 is it a transcription factor?
combined with other oncogenes or anti-s, p53, sv40, e1f....in colony assay
KO or transgenic mice, if you got positive data from colony and
transplantation
what happened if you knockdown the gene in tumour cell lines, can you get
derive a cell line? or any other similar type of cancer cells
blot)
related
oncogene?
【在 L******e 的大作中提到】
: The following experiments were finished:
: 1: gene expression is increased significantly in tumor compared with the
: corresponding normal tissue (RT-PCR for mRNA)
: 2: Protein expression in tumor cell is significantly increased (western blot)
: 3: Cell migration assay showed the stimulation in the overexpressing cells.
: 4; Gene silencing indicated the inhibition of some 'stree', which is related
: to the gene.
: What else need to do? Is thre a routine rule for the approval of an oncogene?
: Thanks.
|
