M****e
发帖数: 70 | 1 dude, you scared me:P. to tell you the truth, nothing but
truth, i think so ... well, don't tell boss and buy another
vial then. next time, try not to do that. i suggest you use
a cell storage from Nalgene. add isopropanol into it, put in
the vial with cells (cells are in DMSO and FBS), and put it
@ -80C. it is said to decrease the temp by 1C/hr. after
put it in the -80C two days, you can now transfer it to
liquid N2. when you thaw the tube from N2, put it in 37C
water directly, don't stand outs | r****o
发帖数: 105 | 2 你所有的管子都试过了吗？如果都死掉了，就没办法了。不过我还是建议你
诚恳的跟老板解释解释表示歉意。不过我觉得不能完全算你的责任，因为看起来
没有任何人教过你注意事项。
液氮保存细胞最主要的原则就是“慢冻快化”。如果你的细胞要存很久才会用，
只存液氮就行。如果隔十天半个月就会用到，又不想总是分细胞，你可以在-80度
放几管。-80度下保存几个月是没问题，液氮保存时间长，但是有时候会发生爆炸
什么的很讨厌。
亡
把
大 |
|
