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Biology版 - Re: 一个7k长的cDNA
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ChIP-qPCR control primer求助 UTR DNA
包子求 这里有人买过Origene的expression ready cDNA clone么? 准确性如何?问个基因表达选promoter的问题
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有没有人用multisite gateway做克隆的?请问:基因5’UTR 长度一定会大于25bp吗?(设计Mopholino antisense oligomer)
请问一个gateway clone问题shRNA resistant mutant
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话题: utr话题: cdna话题: primers话题: clone话题: gene
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1 (共1页)
M****e
发帖数: 70
1
this is a BIG problem. so what is the gene? and from what
kind of organism would u like to clone it? i have only a
couple of suggestions. first of all, check the RACE primers.
have you tried several different primers? 2nd, check the
known EST database and see whether there are severa image
clones available with which you can actually assemble the
clone. this gene might have very long 5'UTR. you may not
need the whole sequence for expression, though the UTR may
have regulatory sequences. 3rd, you
1 (共1页)
进入Biology版参与讨论
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