z****e
发帖数: 54598 | 1 rich web application 不用download anything
你给zkss，这我还是第一次听说 |
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z****e
发帖数: 54598 | 2 那好，说正经事
你得先说说这个rich web application想要做什么
目的是啥？ |
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z****e
发帖数: 54598 | 3 你要rich到什么程度？没考虑过上applet？ |
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g*****g
发帖数: 34805 | 4 Any java ajax framework. The first thing is to make sure it has
all the rich components you need.
spring |
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z*p
发帖数: 43 | 6 还是太小众。刚学习java，准备做一些有网络desktop software。突然看到介绍rich
client， thin client的东西。但是好像只有很少资料介绍。是不是过时了。 哪对应
RCP等有些什么好用的结构或者tools |
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j*****q
发帖数: 82 | 7 rt
how to amplify extreme AT-rich sequences on genomic DNA?
followed some literatures to decrease the enlongation temp. to 60 degree,
but still failed.
i am using the roche polymerase and qiagen one.
seeking for helps!
please!!! |
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g*****n
发帖数: 241 | 8 我想测序的那个Gateway vector在我插入片断之前的序列高度AT rich，大概前180个碱
基里面AT含量高达80%。请问除了设计反向引物，其它有没有什么办法测序啊？谢谢 |
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w*********n
发帖数: 439 | 10 各位大侠,
请问版上有谁在按照Rich Young的CHIPprotol在作的？我发现拿Young的Wash Buffer
RIPA 洗不干净beads，有人有同样感觉吗？ |
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w*********n
发帖数: 439 | 11 多谢指教~
我看到别的protocol比如Upstate的protocol是用low salt, High salt, Licl, TE
这4个buffer 各洗2遍， 但是Rich Young 的protocol是用RIPA/Licl这一种buffer洗4-
7遍
我有点怀疑。 |
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w*********n
发帖数: 439 | 12 Chip拉下来的sample，
就是不该有TF结合的地方，却能做出PCR band.
你一般拿RIPA buffer洗几遍能洗干净？
如果你的没问题那就可能是我自己的问题，我重配Rich Young的RIPA buffer再试一下
，多谢指教~ |
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n***7
发帖数: 10 | 13 微信很火，我看得手痒，一鼓气花了N个晚上，做了一个iPhone/iPad 离线版的微信。
其实也不能说是微信，它只是微信界面风格的记事本！
这个记事本最主要特点是能够在离线状态下工作，不需要WiFi就能把事情存放到机子里
，可以按不同的主题归类，就好像微信上的群组，每个主题上都可以加入文字，声音，
照片，位置，手绘等，它们会按时间先后排列。记事本可以整体加锁，或者单个主题加
锁。随时可以加上一个事件提示主题，把所有的约会，生日等日期加上去。
这是它的网址：
https://itunes.apple.com/ca/app/rich-memo/id850846940?mt=8 |
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c****1
发帖数: 215 | 14 从剧本，演员，剪辑，配乐，服饰，都超级棒!!
当时在优酷里随便点开的，并不知道卡司是谁，结果一看是小栗旬（我不是小栗旬的粉
丝），看着看着觉得配乐很好，尤其在第一集中小栗旬得知女主的名字是泽木千寻时，
那段音乐配上小栗旬当时的演技超级棒，大家可以在youtube上搜“rich man poor
woman late night”，搜出来的第一个视频就是那段ost-.-''!!
miwa唱得主题其实一般，声音主要还是后期修的，不过整体还是很棒的!
特别喜欢女主的演技，之前有在toma酱的《人间失格》中扮演卖烟的小妹，很可爱的说
，在这部剧中演的很讨喜，刚开始看她的嘴唇有够丰满的，一到她的脸部特写镜头，我
都会不由自主地看她的嘴唇，哈! 小栗旬剧中很帅，社长年轻又多金，关键长相英俊身
高184cm, 感情略微有些迟钝对工作投入身心，现实生活中会有这样的社长么? （思考
中=.=!!）还有在大家关注男主女主的同时，突然发现和女二号一同在饭店工作的男厨
师很眼熟，结果发现竟然是《NANA》中扮演光头泰的丸山智己。
导演拍的很紧凑，剪辑师更赞!几乎没有多余的镜头，喜欢这样的日本时装剧!! 月九收... 阅读全帖 |
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c****1
发帖数: 215 | 15 小栗旬在《gto》出演的是一位饱受同学欺负的孩纸，后来看到有报道说，一直以来在
剧中都是小栗旬欺负别人，没见过他被别人欺负过。哈哈!! 当时的旬很萌，还在变声
期之前～okok！！刚在优酷搜了一下，第十集的《rich man poor woman》已经出了生
肉版的了!!坐等晚上的熟肉版!!!! 下周就是完结篇了，期待期待!!!!!!! |
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c****1
发帖数: 215 | 16 完结篇了！期待出rich man poor woman 第二季！社长和妹子终于亲了！！！！ |
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M*********e
发帖数: 1988 | 17 Power law distribution是描述复杂系统中受欢迎程度（popularity）的一种非常常见
的分布。比如，统计数据得出，卖出去k本的书籍数目跟1/k^r成正比，其中r为一常数
，k为变量。有k条被指向链接(in link)数目的网页跟1/k^r成正比，等等。这个分布的
一个重要的特征是它的 heavy tail。也就是说，它描述了复杂系统中某些节点非常受
欢迎的必然性。一定有些书籍是热卖书籍，一定有一些人物家喻户晓，一定有一些歌曲
烂大街，一定有一些人手中聚集了大量财富，等等。
Barabasi在1999年的《科学》论文 [1] 中，第一次给出一个描述此现象的过程，通常
叫做rich-get-richer，或者选择性加入模型 (preferential attachment model)。我
们以互联网网页的链接数为例。在他的模型中，假设n个网页逐一加入到系统中，每一
个新加入的网页以概率p随即选择链接到一个现存网页，以概率1-p随机选择一个现存网
页并复制该网页的链接，即指向此网页所链接的网页。换个方法说，以概率1-p选择链
接到一个网页的可能性跟该网页现存链接数成 |
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H*****L
发帖数: 5705 | 18 seems like 30-40% cars on street are B/B, some audi, landrover and
porsche. small rich weird town.... |
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w*******y
发帖数: 60932 | 26 Okay, so this is my first time posting a deal, so let me know if I'm doing
this correctly.
Amazon has Optimum Nutrition 100% Whey Gold Standard in Double Rich
Chocolate for $32.04 after Subscribe and Save. It's an okay price, as the
normal S&S price is about $35.
Link:
http://www.amazon.com/Optimum-Nutrition-Standard-Double-Chocola
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w*******y
发帖数: 60932 | 27 Free Kindle ebook: Today We Are Rich
Book Description:
Are you just hanging in there? Have lifes curveballs thrown you off balance?
Do you feel as if your life is going sideways?
Bestselling author, leadership coach, and former Yahoo! executive Tim
Sanders knows how you feel. His fathers unexpected death put him in a
downward spiral for fifteen yearswhat he calls his sideways years. A photo
of a dusty water tower in Texas in finally woke him up in 1996. Thats when
he realized he needed to... 阅读全帖 |
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w*******y
发帖数: 60932 | 28 PLEASE NOTE, AMAZON STATES THE ITEM(s) WON'T SHIP FOR 1-2 MONTHS. DON'T
PLACE AN ORDER IF YOU NEED PROTEIN SOONER THAN LATER.
Follow the link below. Don't change any of the options. It only works for
the Double Rich Chocolate Flavor. And make absolutely certain the seller is
Amazon.com and not a 3rd party seller. The poor reviews are from people
who bought from a 3rd party seller, who didn't honor the deal. It's
probably a price mistake, but Amazon is a good company and should honor it.
Ti... 阅读全帖 |
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w*******y
发帖数: 60932 | 30 Superfoods Diet: The 11 Best Nutrient Rich Foods For Increased Immunity,
Energy and Longevity:
http://www.amazon.com/Superfoods-Diet-Increased-Longevity-ebook
Product Description
Superfoods are the planet's best natural foods when it comes to nourishing
your mind and body. They have the ability to transform your health and life.
And, they are affordable and accessible.
In this book, you will find the best superfood for:
- Overall Well Being Feelings/Natural Mood Booster
- Anti-Aging and Reverse ... 阅读全帖 |
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d****g
发帖数: 702 | 32 把rich概念simplify啦，有点坑爹。
不过，抽根烟骗骗自己的，不如拿这个骗自己。lol |
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发帖数: 1 | 34 Christian vs Buddhism.
------How Christian Defines Poverty ------
Spirit of poverty is a spirit of Satan. It is a spirit that uses poverty to
steal kill and destroy.
Having worldly materials, riches and wealthy does not mean you are rich –
instead all worldly materials are vanities.
You may have worldly riches but be poor – have the spirit of poverty
A person may be lacking worldly riches but be rich – not have the spirit of
poverty.
Jesus lacked worldly riches when He was on earth (Mt 8:20) but... 阅读全帖 |
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发帖数: 1 | 35 Stone Cold Word
Longboat Key, Florida4m ago
AG Barr investigating a missing Twinkie is a joke.
So I am reading all these comments laughing and calling out "conspiracy
theories". It's easy to "throw shade"..... getting "shade to stick" is
another story.
I know this. A high profile prisoner, who supposedly tried to kill
himself just two weeks ago, this guy could not get near a 6 inch piece of
thread. Now that's not a "conspiracy theory".
The "shade slingers" want us to believe that this ego ... 阅读全帖 |
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发帖数: 1 | 36 成语新解
1）低三下四
较音：design chance
释义：谋划一个机会，当你想要得到一个机会的时候，就要向对方怎么样呢？RICH连声
说明白明白。
2）东施效颦
较音：dance shopping
释义：跳着舞去shopping,给人的感觉是什么呢？RICH说是a good idea,我说nonono。
后来经提示他终于想起我教给他的一句北京话shaB，我说yesyes。
3）千军万马
较音：changing warmer
释义：当千多个human和万来匹horse挤在一起会怎么样呢？RICH说会很难受，我告诉他
会很暖和，他说有道理。后来他记住了这个成语的发音，当问他意思时，他却告诉我很
多人很多马挤在一起取暖。我差点当场晕倒，这不能不说是我教育工作中的一个败笔。
4）万恶淫为首
较音：while in well show
释义：这个词实在很难解释，我也伤透了脑筋。最后只有用译法让RICH明白，当你IN的
时候，你还把它当成well show，所以说胡乱地xxxx是最evil的。RICH说他能明白我的
意思，可他不赞同我的说法。人都性解放几十年了，我还跟他说这个，这不找啐嘛。
... 阅读全帖 |
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W*******n
发帖数: 4140 | 37 Has Donald J. Trump Actually Hurt You?
Limin Wang
10/18/2016, finalized on 11/5/16
The political opponents and many of the mainstream media have
painted Donald J. Trump as a dude unfit for the U.S.A. presidency
with a variety of brushes and paint. Please allow me to lead the
talk on some issues. His history of actions, not just words, should
give us more clues.
1) Is Donald J. Trump a white supremacist? an immigrant phobicist?
Black neurologist Ben Carson is neither crazy nor stupid, and
he appe... 阅读全帖 |
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G******e
发帖数: 9567 | 38 Luke 6:24
GB: 但你们富足的人有祸了。因为你们受过你们的安慰
Big5: 但你們富足的人有禍了．因為你們受過你們的安慰。
LZZ: 但你们富足的人有祸阿！因为你们己经收到了你们的安慰。
New International Version (©1984)
"But woe to you who are rich, for you have already received your comfort.
New Living Translation (©2007)
"What sorrow awaits you who are rich, for you have your only happiness now.
English Standard Version (©2001)
“But woe to you who are rich, for you have received your consolation.
New American Standard Bible (©1995)
"But woe to you wh... 阅读全帖 |
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l****z
发帖数: 29846 | 39 January 18, 2012 by Warner Todd Huston
I have to say, I am getting a bit sick and tired of this nonsensical lament
about how rotten it is that those running for president are “rich” people.
Stop it right now, America. The fact is that we’ve never really had a poor
man as president so talking about it as if it is news that rich people
often seek the presidency is stupid. Not only that, but today it is
impossible for a poor or even middle class man to run for president anyway,
so get this populist... 阅读全帖 |
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P*********0
发帖数: 4321 | 40 The Bible on the Poor
or, Why God is a liberal
The Bible contains more than 300 verses on the poor, social justice, and God
's deep concern for both. This page contains a wide sample of them, and some
reflections. It's aimed at anyone who takes the Bible seriously.
As you read these passages, you will very likely feel a good deal of
resistance (possibly at first manifesting itself as indifference). American
churches have departed strongly from Biblical values in these areas, and
even created a r... 阅读全帖 |
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l****z
发帖数: 29846 | 41 Connecticut to super-rich residents: Please don't leave us
February 9, 2015 - 4:06 PM
By STEPHEN SINGER, Associated Press
HARTFORD, Conn. (AP) — If you're a billionaire living in Connecticut,
chances are the tax department is keeping an eye on you.
In a state home to some of the richest Americans, tax officials go to some
lengths to keep them — or, more accurately, keep the billions of dollars in
revenue their income taxes generate.
Connecticut tax officials track quarterly estimated payments of... 阅读全帖 |
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p******n
发帖数: 2449 | 42 How I Met the Biggest Devil
Marc Rich, the world’s most powerful oil trader who had systematically
avoided reporters and had given his last interview over twenty years ago,
finally opened up about his businesses and his private life.
Just before Christmas, two years ago, I waited at the Suvretta ski lift in
St. Moritz, the world’s oldest and most glamorous ski resort. It was one of
the coldest mornings of the year, eight degrees below zero Fahrenheit, and
my breath hanged before me in a cloud of... 阅读全帖 |
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q*d
发帖数: 22178 | 43 ☆─────────────────────────────────────☆
swjtuer (swjtuer) 于 (Tue Mar 30 23:56:30 2010, 美东) 提到:
星期一
早餐 牛奶，煮鸡蛋，玉米馒头，榨菜
早点 芦柑
午餐 中大：绿豆饭，木须肉，海米冬瓜，棒骨菠菜汤
小婴：绿豆饭，三叶瓜炒肉，海米冬瓜，棒骨菠菜汤
午点 冰糖山楂水，蔬菜饼
晚餐 棒骨冬苋菜瘦肉粥，卤肉秋叶夹馍
星期二
早餐 牛奶，蒸鸡蛋，枣泥糕
早点 香蕉
午餐 中大：胡萝卜饭，芹菜炒牛肉，珊瑚豆腐，棒骨凤尾汤
小婴：胡萝卜饭，芹菜炒肉，珊瑚豆腐，棒骨凤尾汤
午点 果汁，卤猪肝
晚餐 豌豆肉末焖饭，番茄虾皮紫菜汤
星期三
早餐 南瓜粥，卤蛋，如意肉卷
早点 脐橙
午餐 中大：金银饭，咸烧白，海米儿菜，棒骨冬苋菜汤
小婴：金银饭，樱桃肉，海米儿菜，棒骨冬苋菜汤
午点 牛奶，蛋卷
晚餐 米饭，蒜苔炒肉，芙蓉蒸蛋，棒骨海带萝卜汤
星期四
早餐 牛奶，蒸鸡蛋，奶香馒头
早点 苹果
午餐 中大：南瓜饭，什锦鱼丸，韭菜炒豆干，番茄菌类鱼汤
... 阅读全帖 |
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l****z
发帖数: 29846 | 44 By John O'Sullivan
March 14, 2012 4:00 A.M.
Where is conservatism heading in the English-speaking world? This question
is currently being answered in the four countries of the Anglosphere —
namely, the U.S., Canada, Australia, and Britain — in four distinctive ways.
THE UNITED STATES
I will assume that American readers have a rough grasp of the state of
conservatism in the U.S. as revealed in the Republican primaries. It is
currently — as it has been for many years — a struggle for dominance in
... 阅读全帖 |
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g*******6
发帖数: 1034 | 45 郎五们懂不懂英文？舔，就接着舔吧！Fake News!!!床破犯才是fake news最大的制造
者。
It took only hours for one of the biggest stories in conservative media this
week, which some outlets had chosen to focus on over news that President
Trump disclosed classified information to senior Russian officials, to fall
apart.
On Monday night, Rod Wheeler, a private investigator and Fox News
contributor, put things in motion during an interview with WTTG-TV, a Fox
affiliate in Washington.
Wheeler, who was under contract with the family of... 阅读全帖 |
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j**4
发帖数: 10425 | 46 that's because u r in competition with rich americans, rich chinese, rich
koreans, rich japanese, rich thai, rich iranians, rich indians, rich dubai-
nese? rich UAEs, rich french, rich english, rich spanish, rich mexicans... |
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