B******1
发帖数: 9094 | 1 How about brand new reagent bottles at chemistry or biology labs @ TSRI? They are free, if the boss pays the bill! |
|
c********g
发帖数: 2075 | 2
原来不是我一个人这么干的，汗一个。
del sol有一个公共的BBQ的地方，在靠近reagents的那边。也经常看见住三楼的邻居在
阳台上烤肉的，我也心动过，不过住在二楼怕楼上的邻居抗议，而且烟会跑到屋子里来
，不比三楼的阳台是露天的。 |
|
S***A
发帖数: 40 | 3 5th SABPA/ACS Chemistry Symposium
DATE: Friday, April 10, 2015
TIME: 4:45 – 5:45 PM Registration, Networking and
Light dinner
5:45 – 6:30 PM Dr. Shifeng Pan, “Discovery
of porcupine inhibitors targeting Wnt signaling in cancer”
6:30 – 7:15 PM Professor John F. Hartwig,
“Metal-Mediated and Metal-Catalyzed Incorporation of Fluorine into Aromatic
Molecules”
... 阅读全帖 |
|
l****n
发帖数: 844 | 4 【 以下文字转载自 Biology 讨论区 】
发信人: linden (说LP不对的参见第一条), 信区: Biology
标 题: 湾区biotech startup招RA
发信站: BBS 未名空间站 (Tue Feb 3 14:14:07 2015, 美东)
Research Associate, Assay Development
AccuraGen Inc. is a silicon valley based early-stage biotech startup
focusing on noninvasive diagnostics to facilitate personalized cancer
treatment. We are developing systems providing the most sensitive,accurate,
and robust NGS based ctDNA mutation detection, delivering unparalleled
accuracy from a minimal amount of patien... 阅读全帖 |
|
q******n
发帖数: 4479 | 5 他们好像看房次数有限制吧。另外随便找个agent也有1% cashback了，他们真心不算多
的。
不过他家的网站真心作的好，这是REagent的终极杀手阿。 |
|
t*****t
发帖数: 2 | 6 Level commensurate with experience and qualification
Functions and duties including but not limited to:
1) Order and organize equipments, reagents and supplies;
2) Maintain, calibrate and order service for lab equipments;
3) Assist specific experiments and projects;
4) Lead small pilot projects.
Required skills: DNA/RNA extraction, qRT-PCR, Western blot , tissue
culturing, gene silencing and over-expression in mammalian cells,
immunofluorescent staining, immunohistochemistry, and confocal micros... 阅读全帖 |
|
T******y
发帖数: 14506 | 7 Career
Biotech Sales Representatives
Celltechgen LLC, a Houston-based biotech company, is looking for several
Sales Representatives who will seek out and discuss potential projects with
scientists and researchers to bring new work into the company.
Celltechgen LLC provides laboratory reagents and instruments for life
science research. Our products include Elisa Kits, Antibodies, CRSPR gene
editing kits, Cell-based and beads-based assay kits, covering immunology,
Cancer, Neuroscience, Stem cel... 阅读全帖 |
|
|
|
|
J********i
发帖数: 50662 | 11 咣当。。。别出人命了。。。这里的reagent alcohol有10%甲醇 |
|
l*******y
发帖数: 3987 | 12 已经都忙死了，今天到现在已经工作了９个半小时了：（
老板又加了一个LAST MINUTE的PROJECT，大家帮偶发动小宇宙吧，如果星期三收不到
REAGENT的话，就赶不上DEALINE了，就不用着急做了。。。。。。。。
到时候偶来发２５个双黄包。。。。。。。。 |
|
B*****s
发帖数: 381 | 13 第一个，俺不记得了。
第二个，是花神最喜欢的Daybreaker。我发现花神对颜色（除非很奇特），花形和香味不是特别注重，但是对花序结构特别关注。他喜欢Floribunda类的玫瑰，这个daybreaker就是其中之一，但是它不同于一般的floribunda，花枝短小过于簇拥；它的花茎很长，不仔细看仿佛就是单颗玫瑰，其实再往下看是好几个花枝簇拥在一起。所以花多而且适合扦插。
第三个是nancy reagent，颜色和第二个很像。但是花序上就比较簇拥。 |
|
d**********n
发帖数: 3634 | 14
or
用网站/人代购啊
这年代有买不到的东西么？
The only limiting reagent is $ |
|
a********6
发帖数: 14468 | 15 kill worm reagent
呵呵，应该是pesticide,有不同种类，建议去沃尔马。 |
|
a**********2
发帖数: 3726 | 16 I guess part of the reason is because with time, people's personality
changed since you will have to deal with many bs faculties; daily small
volume pipette of antibodies or reagents make a MAN cheap and narrow minded;
always criticize other people's work and publication makes a person always
argue/criticize in life; ” occupational illness”, when you do this kind of
work everyday, your personality will change subconsciously. Sad! |
|
f**********r
发帖数: 18251 | 17 【 以下文字转载自 Military 讨论区 】
发信人: smartkevin (PE), 信区: Military
标 题: Re: 普鲁士蓝解毒机理
发信站: BBS 未名空间站 (Wed May 1 11:49:11 2013, 美东)
为什么一帮人就喜欢动嘴皮子不喜欢动手狗一下？明明说了是Fe4[Fe(CN)6]3，哪来的
钾？
https://en.wikipedia.org/wiki/Prussian_blue
Prussian blue
From Wikipedia, the free encyclopedia
This article is about the pigment. For the musical duo, see Prussian Blue.
See also: Midnight blue
Prussian blue
IUPAC name[hide]
Iron(II,III) hexacyanoferrate(II,III)
Other names[hide]
Berlin blue
Ferric ferrocyanide
Ferric hexacy... 阅读全帖 |
|
d********u
发帖数: 386 | 18 Could you please give the name of the teacher who said so? And the link
stating this.
As a chemist, I do not believe that "铊" is public accessible. Let me tell
you some common sense in research labs. "No PI (or Boss) buys and keeps
such a poisonous stuff in the lab if he does not need it in his research
projects. And it is absolutely forbidden to leave such dangerous stuff at
public site."
I understand that somebody might use reagent including "铊" in the lab with
UV/VIS, but this is very d... 阅读全帖 |
|
f**********r
发帖数: 18251 | 19 【 以下文字转载自 Biology 讨论区 】
发信人: xlxnr (xlxnr), 信区: Biology
标 题: 分享一个遗传所PI管理实验室的规定
发信站: BBS 未名空间站 (Mon Jun 3 14:28:32 2013, 美东)
来源于http://sourcedb.cas.cn/sourcedb_genetics_cas/zw/zjrck/200907/t20090721_2130979.html， 点击Notes for students 2013。
Notes for Graduate Students
Performance
Progress: if you are unable to make any significant progress on
your project
within a reasonable timeframe (e.g., 1-2 months), you are definitely in
trouble
Efficiency: your competitors would appre... 阅读全帖 |
|
|
q**x
发帖数: 1636 | 21 Material Safety Data Sheet
Sulfuric Acid
ACC# 22350
Section 1 - Chemical Product and Company Identification
MSDS Name: Sulfuric Acid
Catalog Numbers: S71211SC, S71826, S79200, S80213, S80213-1, A298 212,
A298212, A300 212, A300 225LB, A300 500, A300 612GAL, A300 700LB, A300212,
A300225LB, A300500, A300612GAL, A300700LB, A300C 212, A300C212, A300C212001,
A300C212002, A300C212003, A300C212004, A300C212005, A300C212006,
A300C212007, A300C212008, A300C212009, A300C212010, A300C212LC, A300FP 500,
A30... 阅读全帖 |
|
q**x
发帖数: 1636 | 22 Material Safety Data Sheet
Sulfuric Acid
ACC# 22350
Section 1 - Chemical Product and Company Identification
MSDS Name: Sulfuric Acid
Catalog Numbers: S71211SC, S71826, S79200, S80213, S80213-1, A298 212,
A298212, A300 212, A300 225LB, A300 500, A300 612GAL, A300 700LB, A300212,
A300225LB, A300500, A300612GAL, A300700LB, A300C 212, A300C212, A300C212001,
A300C212002, A300C212003, A300C212004, A300C212005, A300C212006,
A300C212007, A300C212008, A300C212009, A300C212010, A300C212LC, A300FP 500,
A30... 阅读全帖 |
|
|
i****a
发帖数: 36252 | 24 中国人表现爱国的时刻已到!
家里有美国车的自觉砸车，别等小将们上门，到时场面失控把你房子也砸了。
在美国上学的小留以不交学费，不买豪车来抗议!
老留千老们以不跟protocol乱做实验，浪费 reagent 来打击美国的科硏发展!
发考题们要努力拿房顶但不要出结果。要以拃干NlH经费为硏究方向!
码工们要坚持上班不出码，出码都是 bug，有 bug 不去修的原则。编码水平要向印度
码工看齐，别做美帝的奴隶! 特别是FLG等几大公司的中国人，把 facebook 搞跨让美
国人不能散布反华信息，把 uber 搞让美国人寸步难行，把 apple 搞跨拯救国人肾，
把 twitter 搞跨让川普不能发言!
地产代理们要联合起来，斉心合力地写 low ball offer，让十年前的房地产危机再现
，把美帝的经济从地产业上拖跨
中国人一定要齐心，爱国的就转发! |
|
t*****t
发帖数: 27 | 25 Avoid RNase contamination. UseDEPC-treated autoclaved H2O to dissolve RNA and
to make all the reagents for RNA assays.Wear gloves
Avoid repetitive freeze-thaw
窍 |
|
l***a
发帖数: 170 | 26 In fact, I think we really should feel ashamed for 人名反应
. because 30-40 years ago, we still have 1/3 chinese 人名反
应 , from
then on a lot of new 人名反应 and 人名 reagent 人名
catalyst appears, there are still no chinese name.
Anyway, it seems a chinese prof in IU named Shi YI An may
break this deadlock. his chiral epoxidation catalyst is
very promising. |
|
A****A
发帖数: 270 | 27 【 以下文字转载自 Boston 讨论区 】
发信人: AGATHA (Agatha Christie), 信区: Boston
标 题: 6月14-15日 复旦人相约查尔斯河
发信站: BBS 未名空间站 (Sat May 3 17:20:18 2014, 美东)
6月14-15日 复旦人相约查尔斯河
波士顿龙舟赛是波士顿华人一年一度的体育盛事，今年的第35届龙舟赛将首现上海复旦
大学代表队！这支年轻的队伍将在6月14-15日那个周末首航查尔斯河！如果您刚得知这
一喜讯，想加入到训练队伍中来，重温大学时代集体备赛那种紧张刺激，赶快报名还来
得及；如果您只想一睹复旦人今日的风采，那比赛当天的啦啦队一定少不了您；如果您
以复旦人为荣，想为队伍出一份财力，无论多少我们都深表感谢！
报名训练及参赛信息：报名费$50，包含六次专业教练指导，时间从5月19日至6月13日
的周一至周五下午6-8点，每次一小时。我们将安排六次集体训练时间，临时不能参加
的同学可以协调进别的时间段参与训练。但龙舟赛为集体项目，讲究团队合作，因此本
着少数服从多数的原则，我们将选取最适合大家的时间集体训练。最终确定... 阅读全帖 |
|
A****A
发帖数: 270 | 28 6月14-15日 复旦人相约查尔斯河----后援团火热招募中
波士顿龙舟赛是波士顿华人一年一度的体育盛事，今年的第35届龙舟赛将首现上海复旦
大学代表队！这支年轻的队伍将在本周末（6月14-15日）首航查尔斯河！两轮分组赛将
于周六在MIT河段进行，紧张刺激的决赛在周日的Harvard河段展开角逐。在NeoBiolab
和NovoProtein 两家生物公司及“佛伦汉服”的联合赞助下，复旦队员将身着传统汉服
集体出战！届时我们在沿河观战绝佳位置有展位及休息区，现诚招热情如火的你加入我
们的后援团为复旦加油助威。请联系 y**********[email protected] 加入我们复旦后援团。
比赛具体赛程及节目请参见如下官方网站：
http://www.bostondragonboatracing.org 龙舟节是波士顿地参与人数最多覆盖范围最广的华人年度文化盛事，将有众多团体沿河亮相，提供丰富多彩的文艺表演，食品品尝，游戏抽奖等形式多样丰富多彩的有趣活动，欢迎带上家人，叫上朋友，一起来度过一个难忘的愉快周末！别忘了，我们复旦校友会不仅派出了我们的史上首只参赛队伍，也有展位和休息区供和新老校友... 阅读全帖 |
|
h*******x
发帖数: 12808 | 29 完全不知所云
大牛普及一下。
Sodium Hydride
Peking University, Shenzhen, China 518055
NaH) as an oxidation promoter have long been overlooked. Uncovered here are
some unprecedented reactivities of this classical reagent under very mild
conditions, including alco |
|
j****1
发帖数: 15497 | 30 a cross section from wild-type Arabidopsis stem tissue stained with Maüle
reagent to show syringyl lignin content. |
|
l******k
发帖数: 27533 | 31 【 以下文字转载自 Pharmacy 讨论区 】
发信人: lilybank (炎炎夏日何处去，采得青莲一茎香), 信区: Pharmacy
标 题: Re: 急问，妈妈睡眠不好，请问有什么好的保健药么？
发信站: BBS 未名空间站 (Tue Mar 13 21:51:34 2012, 美东)
nice search from Beer's list.
I don't look at it, but I believe the first generation antihistamine is not
contraindicated in the elderly. Reference is black and white, but the
patient is specific, the appropriate recommendation should be made based on
the patient's situation. Antihistamine is not the best drug for the elderly
insomnia, but for th... 阅读全帖 |
|
h*i
发帖数: 3446 | 32 去年底的调查表明，78%以上的Clojure用户是在用Clojure来写Web。
Clojure的哲学是用简单小型的模块来自己组装应用，所以node, rails, djingo那种包
含一切的Web框架不是很流行，不过也是有的，比如说immutant，底层用的是undertow。
在server端的，ring是大部分Clojure Web共用的底层模块，在ring的基础上，
compojure是常见的routing模块， hiccup是很常用的html DSL，等等。其他的各种web
有关的小的库很多，几乎什么功能都有几种不同的选择，看你自己的需求。
Browser端，现在基于react.js的ClojureScript框架很火（om和reagent），原因是
clojurescrip的immutable数据可以让diffing算法比native javascript要快很多。
这样的话，基于react.js的clojurescript框架比react.js本身还要快，听起来这是很
counter-intuitive的，但理解了immutable data, 就会发现其实很自然。di... 阅读全帖 |
|
发帖数: 1 | 33 I agree with this guy:
"One note on ClojureScript: you're being unfair to it. Assuming that the
developer has picked up some Clojure basics, the "let" form is just how you
do bindings in Clojure, the dot form is how you interop with *both* Java and
Javascript and "set!" is how you mutate things. This is basic syntax that a
Clojure developer can read. It's a little unfair because there's a
difference between somebody that has never read Clojure and somebody who
does know the language.
If you want... 阅读全帖 |
|
n*******e
发帖数: 27 | 34 biz is a master guy on pcr. i have a little to say. i have been using
tail DNA for pcr characterization of mice KO. my experience is that
u should separate all the reagents/pippets/pippetmen from commonly
used stuff. so, first of all, go to clean the pcr station, and make it
as clean as possible. remember, always NOT to bring template to the
station. also, get pcr buffer/ddH2O changed frequently if u question it.
primers should be stocked as 100uM, and u may only aliquot them for use.
use filter |
|
c*******u
发帖数: 14 | 35 Functional genomics 功能基因组学
鬼子已经几乎完成了水稻基因组的测序工作，
所谓functional genomics是指：
Development and application of global experimental
approaches to assess gene function by making use of
the information and reagents provided by structural
genomics(see Functional Genomics in Plants, 1998,
Plant Physiol. 118:725-732) |
|
R*s
发帖数: 2041 | 36 yes, immortalized cell lines are generally harder to transfect
than primary cells.
however, 283 in general should be easy to transfect unless your
genes are really toxic to the cells (some of genes I have to use
are in such case).
Some guy upstair mentioned Superfect Reagent from Qiagen.
That one is quite effective, and better than LipofectAmine
in many cases, but more expensive too. |
|
M****e
发帖数: 70 | 37 i have never worked with plant, so i do not know whether this
could be due to sth that cuase higher obsorbance at 260nm.
the ratio for isolated RNA from animal tissues is usually 1.7-
2.0 for my experience, and it pretty much depends upon what
kind of kit/reagent i use. i try TRIZol (Invitrogen), which
is pretty good, but sometimes RNA from pancreas will degrade
with no mercy. i also use RNeasy Kit (Qiagen), which is said
to be better, particularly working for microarray purpose.
i would recomme |
|
R*s
发帖数: 2041 | 38 It's called QuickChange Site-Directed Mutagenesis Kit.
Just seach stratagene website for it.
It's very easy to use. One thing is they usually give little
Pfu turbo compared to the other reagents from the kit. So you'd
better buy some extra Pfu turbo yourself if you are doing a lot of this. |
|
R*s
发帖数: 2041 | 39 It's called QuickChange Site-Directed Mutagenesis Kit.
Just seach stratagene website for it.
It's very easy to use. One thing is they usually give little
Pfu turbo compared to the other reagents from the kit. So you'd
better buy some extra Pfu turbo yourself if you are doing a lot of this. |
|
M****e
发帖数: 70 | 40 the essential step is to keep everything as clean as possible.
and do not use anything you are suspicious. Ambion is a good
company that has very good experience dealing with RNA. my
personal experience is that: ALWAYS aliquot reagents. i used
to use DEPC'd ddH2O, but now i would rather use commercial
nuclease free water. try to be a quick and clean hand, quick
and dead, you know that? i.e., you quick and RNase dead, no
no vice versa. when you deal with different biological samples,
you have dif |
|
f***r
发帖数: 204 | 41 还在与PVDF战斗中，再次请教有经验的虾们：
终于看到了条带，但是巨多非特异带，比我要的带都强得多。在网上查到多家western系
统的trouble shooting，里面提到的好几条关于high background的建议让我大开眼界，
又困惑非常，因为矛盾很多：
1. 关于tween的使用：有一家建议blocking之后才用tween，否则tween会stick to the
membrane and cause high noise；但很多常规方法都没有提到这一点。
2. 关于blocking reagents和ab incubation buffer：有说dry milk能reduce
sensitivity，所以如果只用在二抗incubation就能很好降低noise，但也有很多全程用dr
y milk的，也有说dry milk与tween联合使用会提高noise的；有说BSA会造成高背景的，
也有说用BSA可以获得理想信噪比的……
3. 关于ponceau s染色：这次为确证transfer有效，用了ponceau s染色，但发现有资料
说ponceau s就算洗干净也会 |
|
d***e
发帖数: 243 | 42 Trypsin, pepsin 等对LH receptor影响不大{Gospodarowicz 1973}. 所以也可能是其它
的一些原因：cells condition, monolayer overlapping之类的问题.当然commercial
trypsin function本身就比较复杂，有时有些人就用较纯化或重组的trypsin和胶原酶之
类成份确定的混合物去消化。
我的感觉是你是否让你的宝贝细胞刚长得90%confluence后，hanks washing triple,再用
0.05%trypsin在不同温度（Rm, 36）消化一下看有没有提高。
实在不行就定些Cell Dissociation Buffer or Enzyme Free Dissociation
Reagents(60% confluence)，但我总认为是花拳秀腿，但也许就对你的实验管用。
另外培养界面，polypropylene, glass, teflon-coated 都是option,但我对这些不熟悉
。可能还有几个较流氓的方法，冰水超声,内置tiny cover slides, 瞬时低渗 |
|
f******s
发帖数: 115 | 43 可是qiagen说明书有这么一句话：
6. After storage, purify RNA using a QIAGEN kit (see Table 1, page 8).
Be sure to remove tissues from RNAlater RNA Stabilization Reagent prior to
disruption and homogenization in the RNA purification procedure. If tissues
were stored at –20°C, remove any crystals that may have formed.
他们说的”remove any crystals that may have formed“到底是往哪里remove是要还
是不要？而还有个问题，sample在rna later中放置久了会不会造成sample丢失？ |
|
c****l
发帖数: 1086 | 44 http://www.leica-microsystems.com/products/novocastra-reagents/primary-antibodies
Look up here. I cannot remember which one exactly
but we use Rabbit polyclonal anti-Ki67, I think they probably have only one
Rabbit.I can have a look tomorrow and let you know.
Regular IHC protocol,
Mouse brain, 4%PFA fixation O/N
5um paraffin section (or 12um Cryosection)
deparaffination
ph 6.0 Citric acid antigen retrieval 100c 10 min
1 ab 4c O/N
2 ab Rm 1-2hrs
any question, let me know |
|
n********k
发帖数: 2818 | 45 I used primestar from Takara, it is great with my experience...no denaturing
reagents though, otherwise any
Polymerases(I have ever used) would do poorly... |
|
n********k
发帖数: 2818 | 46 google is ur best friend...anyway, in general, it is denature reagent and/or
detergent including DMSO, Betaine, NP40, Triton-X100 etc etc...what I heard
Betaine is likely the best for most of application...and u can buy it from
some company...nonetheless, it could still be tricky even if you know the
gradient, it could still take a hell a lot effort to figure out correct
composition...I personally have used mostly DMSO, and Q solution from qiagen
and a bit betaine... |
|
a****d
发帖数: 1919 | 47 maybe they are talking about packaging plasmids mix, or something like that.
But still, you can figure it out. But if you have to use specific enzymes
for the reaction, like the pENTR system from invitrogen, then you have to
pay for the reagents. |
|
T*G
发帖数: 600 | 48 OMG, you have no idea what you are talking about. The reputation of Yang in
the field is very dominant. Tsien
can't even compare with him. Indeed Yang's most significant work was done
after he got his nobel award,
Yang-miller's field. People followed it got nobel award as well.
Tsien was not even the guy who first discovered GFP as a useful reagent and
his early work was mainly about
in-vivo imaging of Calcium using a better dye. His recent work in labeling
tumor with fluorescent was't a
novel i |
|
T*G
发帖数: 600 | 49 He never allow people to take project or reagent after their postdoc. A
large portion of them can get a AP, but
many of them can't get tenure-ship. And you have to have your own fellowship
within the first year, otherwise
you will be treated bad. if you have other better options, don't go |
|
n********k
发帖数: 2818 | 50 one way to know whether you shall do it or not, is to check how many people
are doing it in their
studies...BTW, as far as I know, what you are trying to do is very very
tricky, and technically rather demanding,
and several companies were trying to develop ways/reagents to control for
this kind of practice...but a Tech
support told me that not many people does it and for most of the people who
have done it, either they are
super great with microscopy work or it is full of craps...In short, it is |
|
