f******s 发帖数: 115 | 1 我提取完组织后立刻放到rna later (qiagen)中 4摄氏度过夜后转到-20度 2周后开始
提取rna 但是发现里面有冰块?还是结晶块?这些东西要还是不要?我后面用trizol法
提 |
r******i 发帖数: 8 | 2 Dosen't matter.
Storage at –20°C can also be used for archival samples. Samples
will not freeze at –20°C, but crystals may form; this will not
affect subsequent RNA isolation. Samples can be stored at
–20°C indefinitely.
From Ambion
【在 f******s 的大作中提到】 : 我提取完组织后立刻放到rna later (qiagen)中 4摄氏度过夜后转到-20度 2周后开始 : 提取rna 但是发现里面有冰块?还是结晶块?这些东西要还是不要?我后面用trizol法 : 提
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h********n 发帖数: 4079 | 3 那个大概是盐的结晶. 把组织从RNAlater里面拿出来, 用Trizol提, 应该没有问题. 我
用这个办法提了很多老鼠的肺. |
f******s 发帖数: 115 | 4 可是qiagen说明书有这么一句话:
6. After storage, purify RNA using a QIAGEN kit (see Table 1, page 8).
Be sure to remove tissues from RNAlater RNA Stabilization Reagent prior to
disruption and homogenization in the RNA purification procedure. If tissues
were stored at –20°C, remove any crystals that may have formed.
他们说的”remove any crystals that may have formed“到底是往哪里remove是要还
是不要?而还有个问题,sample在rna later中放置久了会不会造成sample丢失? |
h********n 发帖数: 4079 | 5 就是把泡在RNAlater里的组织取出来, 用RNase free的水稍微洗几秒, 然后按照常规提
RNA的方法. |