B*******7
发帖数: 137 | 1 1.洗面用品 资生堂的purifying cleansing foam
Good. Use it now.
2. 爽肤水 white lucent brightening refining softener light N
My skin is quite like yours. Tried some sample. But use Benefiance enriching
softener. I love it.
3. 面膜 white lucent brightening massage cream （一个星期1－2次）
Have one. But i think it is not as good as skincare massage cream.
4. 黑头 pureness pore purifying warming scrub （一个星期1－2次）
Use it now. It is so so. |
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l***i
发帖数: 204 | 2 http://www.youtube.com/watch?v=8c4jrLitMC0
以下是她用的所有护肤品：
Day:
St. Ives Green Tea Cleanser
Origins Zero Oil Pore Purifying Toner
Origins Modern Fusion Skin Transforming Catalyst
The Body Shop Elderflower Eye Gel
Lancome Genifique Youth Activating Cream Serum
Lancome Genifique youth Activating Eye Concentrate
Estee Lauder Pore Minimizing Skin Refinisher
La Roche Posay SPF29 Eye cream
Estee Lauder Hydrationist Maximum Moisture Cream
Lioele Sun Shake Lotion SPF 50 PA+++
Night:
Naruko Narcissus Cleans... 阅读全帖 |
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a*****8
发帖数: 281 | 3 在Fashion潜水多年,获益匪浅,也用了很多大家推荐的护肤品,不过甚少发帖,今天也分
享一下我用过的.
我的皮肤: 刚读完大学的时候脸上不停地长痘痘,大概过了2年终于停了..现在夏天皮肤
是混合偏油或油性,冬天是偏干,总之很难侍候. 而且皮肤有时容易敏感,对大多数的
sunscreen和粉底/BB cream/ting moisturizer要么觉得很油,要么发痒或长痘.
Cleanser:
1. Shiseido skincare purifying cleanser. 这个是我用过最最最好的cleanser,洗得
非常干净,有很细很细的磨砂,适合每天晚上用,早上用的话可能就嫌干一点.少量就气泡
,一管可以用很久. 这个我已经用了好几年了,好像自从用了它以后洗脸很干净,然后自
此以后就没有满脸发痘痘了.
2. Philosophy Purity的洗面奶.这是温和派的洗面奶,适合早上用.
http://www.drugstore.com/philosophy-purity-made-simple-one-step
3. Origins Checks and Balance.这个也不错... 阅读全帖 |
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z*****4
发帖数: 1259 | 5 各位JM好，这阵子雅顿一直有活动，想屯点霜以后用。皮肤混合偏油型，起痘，感觉是
外油内干型，我们这边天气太干了，用的东西一定要滋润保湿，但又不能太油，否则脸
上就会出问题，起痘之类的。用EL的time zone或者碧欧泉的skin vivo 还行，太贵的
就囊中羞涩了。没用过雅顿的东西，请问下面这个套装怎么样啊？25%的折扣，满40还
送东西。
Ceramide Plump Perfect Ultra Moisture Cream Set
Full Size of Ceramide Plump Perfect Ultra Lift and Firm Moisture Cream SPF
30, 1.7 oz.
Ceramide Gold Ultra Restorative Capsules Intensive Treatment for Face and
Throat, Total 30 capsules
Ceramide Plump Perfect Ultra Lift and Firm Eye Cream SPF 15, .17 oz.
Ceramide Purifying Cre... 阅读全帖 |
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S**U
发帖数: 7025 | 6 Huh? Read "Turning the Wheel of Dharma Sutra", the first teaching of Lord
Buddha. The Buddha claimed that nothing is beyond his awakening, including
God. There is no need for Buddha to search for God, according to Buddhism.
Gods seeked out Lord Buddha's advice, as many sutras told.
"Turning the Wheel of Dharma Sutra":
"As long as my knowing and seeing how things are, was not quite purified in
these twelve aspects, in these three phases of each of the four noble truths
, I did not claim in the wo... 阅读全帖 |
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a*********6
发帖数: 33 | 7 来美国半年，可我感觉这半年怎么这么长，每天都有点度日如年的感觉，在这边的
半年，吵架伴随了我们无数个日日夜夜，事后总结发现90%都是因为经济问题。
1、 他是在读PHD，每个月固定1300左右的奖学金，由于他家条件一般，父母给不了
他任何支持，我家条件还算行，父母在国内做点小生意。他提出结婚后希望我能过去读
书，但二年MASTER的学费需要我家里负担，他大概估计算了下需要准备50万元，在这两
年还要带每个月500刀的生活费作为备用，以备急需，毕业后他觉得他们专业的年薪会
是9万左右，到时他工资高些了，我家可以不用再负担我的生活费，但以后毕业了如果
要在美买房子的话，他希望我家能出1/3，他以后收入肯定会比我高，所以他说由他来
出2/3。父母觉得只要我和他能幸福，他们会不顾一切的在经济上支持我，读书的学费
和生活费他们全力支持我，以后买房他们只能是尽力支持，他们到时年纪也大了，能拿
得出来就拿，拿不出来只能靠我们自已。父母的爱永远是无私和伟大的，加上我也不甘
以后过去后做HOUSEWIFE，所以过去读书也可以充实自身，将来自已经济也独力一些。
所以来美这半年我就在准备考T，... 阅读全帖 |
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w**7
发帖数: 1265 | 8 JP LG
来美国半年，可我感觉这半年怎么这么长，每天都有点度日如年的感觉，在这边的
半年，吵架伴随了我们无数个日日夜夜，事后总结发现90%都是因为经济问题。
1、 他是在读PHD，每个月固定1300左右的奖学金，由于他家条件一般，父母给不了
他任何支持，我家条件还算行，父母在国内做点小生意。他提出结婚后希望我能过去读
书，但二年MASTER的学费需要我家里负担，他大概估计算了下需要准备50万元，在这两
年还要带每个月500刀的生活费作为备用，以备急需，毕业后他觉得他们专业的年薪会
是9万左右，到时他工资高些了，我家可以不用再负担我的生活费，但以后毕业了如果
要在美买房子的话，他希望我家能出1/3，他以后收入肯定会比我高，所以他说由他来
出2/3。父母觉得只要我和他能幸福，他们会不顾一切的在经济上支持我，读书的学费
和生活费他们全力支持我，以后买房他们只能是尽力支持，他们到时年纪也大了，能拿
得出来就拿，拿不出来只能靠我们自已。父母的爱永远是无私和伟大的，加上我也不甘
以后过去后做HOUSEWIFE，所以过去读书也可以充实自身，将来自已经济也独力一些。
所以来美这半年我... 阅读全帖 |
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d********0
发帖数: 5142 | 9 ☆─────────────────────────────────────☆
apple123456 (apple) 于 (Fri May 27 20:13:13 2011, 美东) 提到:
来美国半年，可我感觉这半年怎么这么长，每天都有点度日如年的感觉，在这边的
半年，吵架伴随了我们无数个日日夜夜，事后总结发现90%都是因为经济问题。
1、 他是在读PHD，每个月固定1300左右的奖学金，由于他家条件一般，父母给不了
他任何支持，我家条件还算行，父母在国内做点小生意。他提出结婚后希望我能过去读
书，但二年MASTER的学费需要我家里负担，他大概估计算了下需要准备50万元，在这两
年还要带每个月500刀的生活费作为备用，以备急需，毕业后他觉得他们专业的年薪会
是9万左右，到时他工资高些了，我家可以不用再负担我的生活费，但以后毕业了如果
要在美买房子的话，他希望我家能出1/3，他以后收入肯定会比我高，所以他说由他来
出2/3。父母觉得只要我和他能幸福，他们会不顾一切的在经济上支持我，读书的学费
和生活费他们全力支持我，以后买房他们只能是尽力支持，他们到时年纪也大... 阅读全帖 |
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t******n
发帖数: 2939 | 10 ☆─────────────────────────────────────☆
apple123456 (apple) 于 (Fri May 27 20:13:13 2011, 美东) 提到:
来美国半年，可我感觉这半年怎么这么长，每天都有点度日如年的感觉，在这边的
半年，吵架伴随了我们无数个日日夜夜，事后总结发现90%都是因为经济问题。
1、 他是在读PHD，每个月固定1300左右的奖学金，由于他家条件一般，父母给不了
他任何支持，我家条件还算行，父母在国内做点小生意。他提出结婚后希望我能过去读
书，但二年MASTER的学费需要我家里负担，他大概估计算了下需要准备50万元，在这两
年还要带每个月500刀的生活费作为备用，以备急需，毕业后他觉得他们专业的年薪会
是9万左右，到时他工资高些了，我家可以不用再负担我的生活费，但以后毕业了如果
要在美买房子的话，他希望我家能出1/3，他以后收入肯定会比我高，所以他说由他来
出2/3。父母觉得只要我和他能幸福，他们会不顾一切的在经济上支持我，读书的学费
和生活费他们全力支持我，以后买房他们只能是尽力支持，他们到时年纪也大... 阅读全帖 |
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O*******d
发帖数: 20343 | 11 加州把下水道的家庭污水净化后再注入自来水系统。 马桶下去的水，从厨房水龙头出
来。 这个是中国的地沟油的美国翻版。
From Sewage, Added Water for Drinking
But on Nov. 30, for millions of people here in Orange County, pulling the
lever will be the start of a long, intense process to purify the sewage into
drinking water — after a hard scrubbing with filters, screens, chemicals
and ultraviolet light and the passage of time underground.
On that Friday, the Orange County Water District will turn on what industry
experts say is the world’s largest plant devo... 阅读全帖 |
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n***9
发帖数: 3 | 12 It's a tightrope the Chinese government would rather not walk, with the
sensitive 10th anniversary of Tiananmen Square just gone by. Falun Gong,
however, is pressing its own issues with communist leaders. The sect, a
popular quasi-religious movement gaining followers worldwide, desires
official recognition and respect. This has precipitated a power struggle
that has tensions rising.
After being caught off guard by the emerging movement's 25 April 1999 ten-
thousand-plus-member sit-in — a silent ... 阅读全帖 |
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m*****d
发帖数: 13718 | 13 Respirator Recommendations
NIOSH
Up to 350 ppm:
(APF = 10) Any supplied-air respirator
Up to 875 ppm:
(APF = 25) Any supplied-air respirator operated in a continuous-flow mode
Up to 1200 ppm:
(APF = 50) Any air-purifying, full-facepiece respirator (gas mask) with a
chin-style, front- or back-mounted canister providing protection against the
compound of concern†
(APF = 50) Any self-contained breathing apparatus with a full facepiece
(APF = 50) Any supplied-air respirator with a full facepie... 阅读全帖 |
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i*****p
发帖数: 2149 | 14 我大概一个月半前又开始过敏，我鼻子本来就不好，一开始过敏就 post nasal drip,
弄得喉咙也坏了。
上周末我妈忽然要来，我全家地板吸了两次，加上用 mop 抹，然后开新买的 Blue Air
的 purifier。 居然鼻子好多了，喉咙已经不痛了。不过不太确定那个 air purifier
有没有用。这机器每次动了10分钟就宣称空气干净了，然后自己减弱到最小。我家灰
尘应该很多才对。。。
你家是地板还是地毯？ |
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s*******5
发帖数: 2503 | 15 多谢，有两个回帖都说到出问题之后好好吸地之后就缓解了，看来勤吸地，吸地彻底是
一个关键。我LD太懒，一个星期吸一次地都保证不了，而且vacuum也是比较弱小的那种。
是地毯，blue air还挺贵的，如果我要败一个好吸尘器，就不败这么好的purifier了。
。。
,
Air
purifier |
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b******a
发帖数: 12216 | 16 ☆─────────────────────────────────────☆
lken (归零) 于 (Thu Jan 13 22:40:02 2011, 美东) 提到:
有谁给猫屎盆子做了通风系统？
猫屎盆子放在厨房几年了，终于受不了满家漂得臭味了，打算
做通风系统fan ventilation system, 把臭味排到窗户外面去.
Here is the prototype idea:
1. Cut 纸箱子 to cover the litter box completely.
2. Install a air pipe from the top box to window.
3. Install a usb fan like this one at end of the pipe:
http://www.amazon.com/Thermaltake-Mobile-Fan-External-Cooling/d
data/B00080G0BK/ref=de_a_smtd
4. Leave the fan running all day long to vent bad ... 阅读全帖 |
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g****x
发帖数: 3862 | 17 原来如此，很困惑呢，如何这么有创意给purifier开发出这么个功能。
也被种草了~~
link 发错了！！！！！ 那个是air purifier ， 这个才是dehydrator:
★ Sent from iPhone App: iReader Mitbbs Lite 7.52 |
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Y****a
发帖数: 17170 | 19
每一种air purifier都有适用多大空间的说明。
放多少，就看你家的空间了。
便宜和贵的主要区别在于，做工，寿命，安静程度，和 filter的功效。
你可以去看看review就知道了。
但是对于那些说可以lifetime reuse filter的 purifier还是回避比较好。 |
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z*****e
发帖数: 14786 | 21 用 air purifier吧，好几个版上养猫但对猫过敏的都用 purifier并有良好的效果
result
of |
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j****n
发帖数: 107 | 22 谢谢seattle2009帮我转文。
我们在西雅图地区。
孩子房间有一个air purifier.
啊 我家已经三只了。。六只还是觉得没有准备好。。应该是在西雅图吧。原帖发在那
边的。祝福猫猫们。同问试过air purifier么？ |
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a**********e
发帖数: 418 | 23 Please do some research and find out what amino acids were made in the
experiment and comment again.
---are you considering these amino acids are the same as what are in our
body? Otherwise we would have a (L,S) body instead of L body
well I can assure you the lab symmetric and assymetric synthesis of amino
acid is well known. One of them is strecker synthesis I happened to work on
one of the project. I can purify the amino acid to any degree of purity
you want by different means. So please b... 阅读全帖 |
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R******d
发帖数: 5739 | 24 Please do some research and find out what amino acids were made in the
experiment and comment again.
---are you considering these amino acids are the same as what are in our
body? Otherwise we would have a (L,S) body instead of L body
--------------------------------------
please be specific on what you are refering to. if you are refering to the
stereogenicity, then you are correct. chirality is one of the unsolved
mystery. However, it is only unsolved at this time. It has nothing to do
wit... 阅读全帖 |
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E*****m
发帖数: 25615 | 25 【 以下文字转载自 TrustInJesus 讨论区 】
发信人: Eloihim (真神), 信区: TrustInJesus
标 题: 清教徒（加爾文信徒）主張宗教自由嗎？ 請所長研究一下。
发信站: BBS 未名空间站 (Thu Nov 24 08:30:16 2011, 美东)
清教徒確實主張自己有宗教自由，但是不主張別人可以有。
對於貴格教派，清教徒主張直接吊死， 而且也確實吊死了一些。
最後還要英國國王派人過來確保貴格派有宗教自由，防止清教徒
壓迫他們。
這種精神有什麼好繼承的？有什麼好說嘴的？
這板的加爾文信徒就是繼承這種精神的。
參考資料
http://en.wikipedia.org/wiki/Puritan#Opposition_to_Quakerism
The Puritans of the Massachusetts Bay Colony were the most active of the New
England persecutors of Quakers, and the persecuting spirit was shared by
the Pl... 阅读全帖 |
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E*****m
发帖数: 25615 | 26 清教徒確實主張自己有宗教自由，但是不主張別人可以有。
對於貴格教派，清教徒主張直接吊死， 而且也確實吊死了一些。
最後還要英國國王派人過來確保貴格派有宗教自由，防止清教徒
壓迫他們。
這種精神有什麼好繼承的？有什麼好說嘴的？
這板的加爾文信徒就是繼承這種精神的。
參考資料
http://en.wikipedia.org/wiki/Puritan#Opposition_to_Quakerism
The Puritans of the Massachusetts Bay Colony were the most active of the New
England persecutors of Quakers, and the persecuting spirit was shared by
the Plymouth Colony and the colonies along the Connecticut river.[32] In
1660, one of the most notable victims of the religious intolerance was
English... 阅读全帖 |
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m****s
发帖数: 18160 | 27 ☆─────────────────────────────────────☆
Dramaer (不要问我从那里来) 于 (Fri Oct 22 12:02:52 2010, 美东) 提到:
Act 1：1-11 Jesus Taken Up Into Heaven
1In my former book, Theophilus, I wrote about all that Jesus began to do and
to teach 2until the day he was taken up to heaven, after giving
instructions through the Holy Spirit to the apostles he had chosen. 3After
his suffering, he showed himself to these men and gave many convincing
proofs that he was alive. He appeared to them over a period of forty days
... 阅读全帖 |
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D*****r
发帖数: 6791 | 28 Letters from the Earth
When the Creator had finished thinking, He said, "I have thought. Behold!"
He lifted His hand, and from it burst a fountain-spray of fire, a million
stupendous suns, which clove the blackness and soared, away and away and
away, diminishing in magnitude and intensity as they pierced the far
frontiers of Space, until at last they were but as diamond nailheads
sparkling under the domed vast roof of the universe.
At the end of an hour the Grand Council was dismissed.
They left... 阅读全帖 |
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B*********e
发帖数: 254 | 29 A Sermon on the Pericope Adulterae
Bible Research > Textual Criticism > Story Of The Adulteress > Quo Vadis?
Quo Vadis?
A Sermon on the Pericope Adulterae
by Michael Marlowe, 2004
The Quo Vadis story is one of those Legends of the Saints that are well-know
n to Catholics but practically unknown to Protestants. It is an ancient lege
nd concerning Peter's martyrdom, believed to be from the second century, and
preserved in the collection of legends included in the apocryphal Acts of P
eter. George ... 阅读全帖 |
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n*******l
发帖数: 110 | 30 Jews do not believe in "Hell" as a place of eternal torment. Gehenna is a
place or condition of purgatory where Jews spend up to twelve months
purifying to get into heaven,[citation needed] depending on how sinful they
have been, although some suggest that certain types of sinners can never be
purified enough to go to heaven and rather than facing eternal torment,
simply cease to exist. Therefore, some violations like suicide would be
punished by separation from the community, such as not being ... 阅读全帖 |
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z********o
发帖数: 18304 | 31
us
年。
要点脸皮行不行？还是你那骠悍的逻辑又开始搅糨糊了？
逻辑很清楚：
1）你承认你有罪
2）你家主子“forgive”基们的“罪”并且“purify”你们基们。“purify”的意思懂
不懂？
所以：
3）“No one who is born of God will continue to sin”
清清楚楚！
这和你前面拿有些情况下法老自己心硬来否认有些情况下你家主子使法老心硬一样弱智。 |
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S**U
发帖数: 7025 | 32 Indeed. Very thoughtful.
Seeing ourselves honestly is not only helpful in romance, but also essential
to spiritual practice that purifies ourselves. You don't apply make-up w/o
looking in the mirror. Likewise, you don't purify mental defilements w/o
seeing them. |
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m*f
发帖数: 8162 | 34 purify很好用, 但是最新的purify不支持最新的visual studio |
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s****a
发帖数: 238 | 35 静态分析对动态语言可能有点用，对静态语言最多作为编译器的补充。
我们的代码在release之前都要跑coverity和purify，coverity报的错误就没几个实质
性的问题，有的还加些蛋疼代码make it happy，倒是purify能找出很多隐患，而且有
些确实隐藏的很深。 |
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M****e
发帖数: 70 | 36 come on, show time, folks. so should we all email assassin back and corrupt
his mail box? i won't discuss all of the questions listed by assasin. actually,
to read papers on Cell is always headache, particularly manuscripts by
biochemistry/cell biology people.
Fig2A shows that purified separase can utilize in vitro translated(IVT) hSCC1
as well as endogenous hSCC1 as substrate in an in vitro cleavage assay. I think
the IVT sample (in RRL?) should be purified first (avoid incomplete translation), |
|
c*******o
发帖数: 8869 | 37 digestion of PCR product can be very tricky, what i do is following:
1 purify your PCR product with qiagen kit
2 use T4 PNK phosphorylate your PCR product's blunt end
3 ligate your blunt PCR fragment into any vector cutted with blunt end enzyme
(like EcoRV)
4 transformation and mini prep plasmid
5 then use hindIII and EcoRI cut the fragment out of vector
6 purify your hindIII-EcoRI fragment with kit and insert it into the vector
you want to use
this will add one more subcloning step but i am sur |
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f***8
发帖数: 571 | 38 Last week's high-profile announcement about a bacterium that thrives on
arsenic is drawing criticism from scientists in multiple fields, including
chemists. The study claims that the microbe can swap phosphorus for arsenic
in its biomolecules, including DNA (C&EN, Dec. 6, page 36; Science, DOI: 10.
1126/science.1197258). But outside experts say the data presented don't back
this claim.
NASA astrobiologist Felisa Wolfe-Simon and her colleagues have been the
subject of worldwide media attention si... 阅读全帖 |
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z***x
发帖数: 80 | 39 I have no experience of isolating RNA from cartilage but need to do it soon.
Cartilage contains a lot of proteoglycans and glycosaminoglycans (GAGs). I
first homogenized cartilage into powders by freeze-milling. I then tried
guanidine and phenol to dissolve the cartilage powder but it was not quite
efficient. Most of the 100mg of cartilage powder was not dissolved in 2 ml
guanidine/phenol (Qiagen tissue lysis reagent).
The other question is that RNA and GAGs are both negatively charged polymers
... 阅读全帖 |
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j****g
发帖数: 406 | 40 Depending on which company you use, you might not need to do that.
I purified my proteins in denatured condition and the company it is fine for
injection. So I just sent them the protein in 8M urea.
And I got quite good antibodies.
The problem is if you are going to affinity purify your antibody.
If you are, then you will need to get protein in native condition.
In my case, I lowered the IPTG concentration and lowered the temperature for
induction and only got a tiny amount of protein.
But that ... 阅读全帖 |
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y*****h
发帖数: 166 | 41 Position level – Research Associate III or Senior Research Associate –
will be determined based on candidate’s background and experience.
Responsibilities and Duties
Responsible for the set up and performance of both in vitro and in vivo
experiments. The candidate will set up and perform in vitro experiments
using purified primary cells from human blood or mouse tissue. The candidate
will be involved in complex in vitro experiments requiring high technical
skills and precise planning. Addition... 阅读全帖 |
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z*******a
发帖数: 175 | 42 my comment on comments of Nemo and Biogene (see below). by the way, I like
these two names.
1. Once upon a time, I did not take any <0.03 % input as a real binding when
I used antisera not purified IgG.
But I saw some papers in top journals have data published like 0.00something
%input, really confusing. do
you two or 达人 have any comments?
2. I would say enrichment folds instead of fold enrichment. I should
calculate folds relative to normal serum
control when antisera are used or IgG control w... 阅读全帖 |
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B*****e
发帖数: 1005 | 43 Thank you for your kind reply.
IgG content of Antibody may not be equal to purified IgG,
Why don't calculate fold enrichment relative to KO cell line using same
antibody?
2. I would say enrichment folds instead of fold enrichment. I should
calculate folds relative to normal serum
control when antisera are used or IgG control when purified IgG antibodies
are used, which means every
single sample must have a normal serum or IgG control, which makes me sick.
So I often times use non
related DNA re... 阅读全帖 |
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c********b
发帖数: 363 | 44 I used Invitrogen GeneRACer kit，which suggested nested-pcr.
Invitrogen's oligo adapter is about 60 nt，first PCR used adapter 5‘-end ~
30 nt primer and gene specific（GS) primer. Then they use close to adapter 3
'-end nested primer and another nested GS primer.
After the nested PCR, I sometimes still see multiple bands. I have to gel-
purify the PCR products with correct size and sometimes to repeat 2nd PCR to
amplify it before cloning.
One suggestion is to purify mRNA for RACE, which gives you mu... 阅读全帖 |
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n***w
发帖数: 2405 | 45 需要用pGEM T-easy这个vector,
pcr是用phusion做的，得到的是blunt end,
问一下3'端加A，
我是直接在PCR reaction里加一点taq呢（看thermo网站说加A前纯化很重要。。。）？
还是说purify以后再用taq，72度20分钟（这一步还要纯化吗？还是直接可以往下做？
）。
我上次用了purified的和taq一起，然后纯化了，然后TA，结果没连上，都是载体自连
。。。
请有经验的说说。
谢谢～～ |
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y******8
发帖数: 1764 | 46 Doesn't matter. Use pure GST to deplete anti-GST antibody, or use protein
with another tag to purify specific antibody.
20kd protein could be GST only, typical with purified GST-fusion protein. |
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a*******u
发帖数: 19 | 47 Cell的一篇paper
http://www.cell.com/abstract/S0092-8674(09)01506-2
的supplementary
Estimation of absolute number of Jarid2 and Jarid1a molecules per ES cell
nucleus.
Purified recombinant Jarid2 fragment a (Figure 2B, details in Table S9)
containing the epitope
recognized by the Jarid2 antibody was used for protein estimation. The
Jarid1a recombinant
protein containing aa191-290 (cat# H00005927-Q01) and the antibody (cat#
H00005927-A01)
recognizing same fragment were purchased from Abnova. Two-fold s... 阅读全帖 |
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D****g
发帖数: 275 | 48 Design primers F/R to cover you gene, then design primers F1/R1 with the
single site mutation to cover the mutation area. Do PCR1 with F/R1, PCR2
with F1/R, then purify the two products, put them in the third PCR tube as
template, with F/R as the primer, do the third PCR, purify the product, and
then clone into your target vector. Done! |
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K*F
发帖数: 120 | 49 请您转发 z******[email protected]
万分感谢！祝您身体健康，财源广进！
Biochim Biophys Acta. 1988 Apr 28;954(1):1-13.
The inducible trimethylamine-N-oxide reductase of Escherichia coli K12:
biochemical and immunological studies.
Silvestro A, Pommier J, Giordano G.
Source
Laboratoire de Structure et Fonction des Biomembranes, CNRS, Marseille,
France.
Abstract
The inducible trimethylamine-N-oxide reductase which migrates on non-
denaturing polyacrylamide gels with an RF of 0.22, has been purified from
the soluble fraction o... 阅读全帖 |
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